Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS03220 and QEN71_RS03225 are separated by 190 nucleotides QEN71_RS03225 and QEN71_RS03230 are separated by 98 nucleotides QEN71_RS03230 and QEN71_RS03235 are separated by 134 nucleotides QEN71_RS03235 and QEN71_RS03240 are separated by 171 nucleotides
QEN71_RS03220: QEN71_RS03220 - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 717,110 to 717,889
_RS03220
QEN71_RS03225: QEN71_RS03225 - tRNA-Gly, at 718,080 to 718,153
_RS03225
QEN71_RS03230: QEN71_RS03230 - YggT family protein, at 718,252 to 718,815
_RS03230
QEN71_RS03235: QEN71_RS03235 - EthD family reductase, at 718,950 to 719,270
_RS03235
QEN71_RS03240: QEN71_RS03240 - DUF2520 domain-containing protein, at 719,442 to 720,380
_RS03240
Position (kb)
718
719 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 717.279 kb on + strand, within QEN71_RS03220 at 717.279 kb on + strand, within QEN71_RS03220 at 717.279 kb on + strand, within QEN71_RS03220 at 717.280 kb on - strand, within QEN71_RS03220 at 717.280 kb on - strand, within QEN71_RS03220 at 717.280 kb on - strand, within QEN71_RS03220 at 717.280 kb on - strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.309 kb on + strand, within QEN71_RS03220 at 717.310 kb on - strand, within QEN71_RS03220 at 717.310 kb on - strand, within QEN71_RS03220 at 717.310 kb on - strand, within QEN71_RS03220 at 717.412 kb on + strand, within QEN71_RS03220 at 717.412 kb on + strand, within QEN71_RS03220 at 717.412 kb on + strand, within QEN71_RS03220 at 717.412 kb on + strand, within QEN71_RS03220 at 717.412 kb on + strand, within QEN71_RS03220 at 717.412 kb on + strand, within QEN71_RS03220 at 717.412 kb on + strand, within QEN71_RS03220 at 717.413 kb on - strand, within QEN71_RS03220 at 717.413 kb on - strand, within QEN71_RS03220 at 717.413 kb on - strand, within QEN71_RS03220 at 717.413 kb on - strand, within QEN71_RS03220 at 717.455 kb on + strand, within QEN71_RS03220 at 717.456 kb on - strand, within QEN71_RS03220 at 717.684 kb on + strand, within QEN71_RS03220 at 717.685 kb on - strand, within QEN71_RS03220 at 717.696 kb on + strand, within QEN71_RS03220 at 717.696 kb on + strand, within QEN71_RS03220 at 717.697 kb on - strand, within QEN71_RS03220 at 717.697 kb on - strand, within QEN71_RS03220 at 717.974 kb on + strand at 717.974 kb on + strand at 717.974 kb on + strand at 717.975 kb on - strand at 717.975 kb on - strand at 717.975 kb on - strand at 717.976 kb on + strand at 717.977 kb on - strand at 717.977 kb on - strand at 717.977 kb on - strand at 717.988 kb on - strand at 717.988 kb on - strand at 718.046 kb on - strand at 718.046 kb on - strand at 718.113 kb on + strand, within QEN71_RS03225 at 718.113 kb on + strand, within QEN71_RS03225 at 718.113 kb on + strand, within QEN71_RS03225 at 718.114 kb on - strand, within QEN71_RS03225 at 718.114 kb on - strand, within QEN71_RS03225 at 718.114 kb on - strand, within QEN71_RS03225 at 718.114 kb on - strand, within QEN71_RS03225 at 718.126 kb on + strand, within QEN71_RS03225 at 718.126 kb on + strand, within QEN71_RS03225 at 718.126 kb on + strand, within QEN71_RS03225 at 718.127 kb on - strand, within QEN71_RS03225 at 718.127 kb on - strand, within QEN71_RS03225 at 718.127 kb on - strand, within QEN71_RS03225 at 718.131 kb on + strand, within QEN71_RS03225 at 718.131 kb on + strand, within QEN71_RS03225 at 718.131 kb on + strand, within QEN71_RS03225 at 718.131 kb on + strand, within QEN71_RS03225 at 718.131 kb on + strand, within QEN71_RS03225 at 718.132 kb on - strand, within QEN71_RS03225 at 718.132 kb on - strand, within QEN71_RS03225 at 718.132 kb on - strand, within QEN71_RS03225 at 718.132 kb on - strand, within QEN71_RS03225 at 718.132 kb on - strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.134 kb on + strand, within QEN71_RS03225 at 718.135 kb on - strand, within QEN71_RS03225 at 718.135 kb on - strand, within QEN71_RS03225 at 718.135 kb on - strand, within QEN71_RS03225 at 718.135 kb on - strand, within QEN71_RS03225 at 718.135 kb on - strand, within QEN71_RS03225 at 718.135 kb on - strand, within QEN71_RS03225 at 718.135 kb on - strand, within QEN71_RS03225 at 718.140 kb on + strand, within QEN71_RS03225 at 718.141 kb on - strand, within QEN71_RS03225 at 718.141 kb on - strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.142 kb on + strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.143 kb on - strand, within QEN71_RS03225 at 718.250 kb on + strand at 718.250 kb on + strand at 718.250 kb on + strand at 718.250 kb on + strand at 718.251 kb on - strand at 718.406 kb on + strand, within QEN71_RS03230 at 718.406 kb on + strand, within QEN71_RS03230 at 718.583 kb on + strand, within QEN71_RS03230 at 718.583 kb on + strand, within QEN71_RS03230 at 718.583 kb on + strand, within QEN71_RS03230 at 718.583 kb on + strand, within QEN71_RS03230 at 718.583 kb on + strand, within QEN71_RS03230 at 718.583 kb on + strand, within QEN71_RS03230 at 718.583 kb on + strand, within QEN71_RS03230 at 718.584 kb on - strand, within QEN71_RS03230 at 718.584 kb on - strand, within QEN71_RS03230 at 718.584 kb on - strand, within QEN71_RS03230 at 718.584 kb on - strand, within QEN71_RS03230 at 718.584 kb on - strand, within QEN71_RS03230 at 718.639 kb on - strand, within QEN71_RS03230 at 718.712 kb on + strand, within QEN71_RS03230 at 718.712 kb on + strand, within QEN71_RS03230 at 718.712 kb on + strand, within QEN71_RS03230 at 718.712 kb on + strand, within QEN71_RS03230 at 718.712 kb on + strand, within QEN71_RS03230 at 718.713 kb on - strand, within QEN71_RS03230 at 718.713 kb on - strand, within QEN71_RS03230 at 718.713 kb on - strand, within QEN71_RS03230 at 718.713 kb on - strand, within QEN71_RS03230 at 718.782 kb on + strand at 718.782 kb on + strand at 718.782 kb on + strand at 718.782 kb on + strand at 718.783 kb on - strand at 719.005 kb on + strand, within QEN71_RS03235 at 719.005 kb on + strand, within QEN71_RS03235 at 719.005 kb on + strand, within QEN71_RS03235 at 719.005 kb on + strand, within QEN71_RS03235 at 719.006 kb on - strand, within QEN71_RS03235 at 719.006 kb on - strand, within QEN71_RS03235 at 719.006 kb on - strand, within QEN71_RS03235 at 719.006 kb on - strand, within QEN71_RS03235 at 719.056 kb on + strand, within QEN71_RS03235 at 719.056 kb on + strand, within QEN71_RS03235 at 719.056 kb on + strand, within QEN71_RS03235 at 719.057 kb on - strand, within QEN71_RS03235 at 719.209 kb on + strand, within QEN71_RS03235 at 719.209 kb on + strand, within QEN71_RS03235 at 719.209 kb on + strand, within QEN71_RS03235 at 719.210 kb on - strand, within QEN71_RS03235 at 719.210 kb on - strand, within QEN71_RS03235 at 719.210 kb on - strand, within QEN71_RS03235 at 719.210 kb on - strand, within QEN71_RS03235 at 719.210 kb on - strand, within QEN71_RS03235 at 719.210 kb on - strand, within QEN71_RS03235 at 719.212 kb on + strand, within QEN71_RS03235 at 719.212 kb on + strand, within QEN71_RS03235 at 719.212 kb on + strand, within QEN71_RS03235 at 719.212 kb on + strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.213 kb on - strand, within QEN71_RS03235 at 719.397 kb on + strand at 719.398 kb on - strand at 719.433 kb on + strand at 719.433 kb on + strand at 719.433 kb on + strand at 719.433 kb on + strand at 719.434 kb on - strand at 719.434 kb on - strand at 719.434 kb on - strand at 719.434 kb on - strand at 719.434 kb on - strand at 719.434 kb on - strand at 719.443 kb on + strand at 719.444 kb on - strand at 719.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 717,279 + QEN71_RS03220 0.22 -1.3 717,279 + QEN71_RS03220 0.22 -0.3 717,279 + QEN71_RS03220 0.22 +1.0 717,280 - QEN71_RS03220 0.22 +0.1 717,280 - QEN71_RS03220 0.22 -1.0 717,280 - QEN71_RS03220 0.22 +1.1 717,280 - QEN71_RS03220 0.22 -1.0 717,309 + QEN71_RS03220 0.26 -0.2 717,309 + QEN71_RS03220 0.26 +0.5 717,309 + QEN71_RS03220 0.26 -0.3 717,309 + QEN71_RS03220 0.26 +0.2 717,309 + QEN71_RS03220 0.26 -1.0 717,309 + QEN71_RS03220 0.26 -0.1 717,309 + QEN71_RS03220 0.26 -1.0 717,309 + QEN71_RS03220 0.26 -1.6 717,310 - QEN71_RS03220 0.26 -0.3 717,310 - QEN71_RS03220 0.26 +0.8 717,310 - QEN71_RS03220 0.26 -0.3 717,412 + QEN71_RS03220 0.39 -0.3 717,412 + QEN71_RS03220 0.39 -0.3 717,412 + QEN71_RS03220 0.39 -0.9 717,412 + QEN71_RS03220 0.39 -0.9 717,412 + QEN71_RS03220 0.39 -0.7 717,412 + QEN71_RS03220 0.39 +0.3 717,412 + QEN71_RS03220 0.39 +0.8 717,413 - QEN71_RS03220 0.39 -0.0 717,413 - QEN71_RS03220 0.39 +0.3 717,413 - QEN71_RS03220 0.39 -0.7 717,413 - QEN71_RS03220 0.39 -1.6 717,455 + QEN71_RS03220 0.44 +1.2 717,456 - QEN71_RS03220 0.44 +1.1 717,684 + QEN71_RS03220 0.74 -0.3 717,685 - QEN71_RS03220 0.74 -1.1 717,696 + QEN71_RS03220 0.75 -0.7 717,696 + QEN71_RS03220 0.75 +0.5 717,697 - QEN71_RS03220 0.75 -0.2 717,697 - QEN71_RS03220 0.75 -0.3 717,974 + +1.5 717,974 + -0.0 717,974 + -0.6 717,975 - -0.5 717,975 - -0.9 717,975 - -0.1 717,976 + -1.0 717,977 - -2.0 717,977 - -0.6 717,977 - -0.8 717,988 - -1.2 717,988 - -0.6 718,046 - +0.1 718,046 - +0.1 718,113 + QEN71_RS03225 0.45 +0.4 718,113 + QEN71_RS03225 0.45 +0.5 718,113 + QEN71_RS03225 0.45 -0.0 718,114 - QEN71_RS03225 0.46 -0.3 718,114 - QEN71_RS03225 0.46 -0.9 718,114 - QEN71_RS03225 0.46 +2.0 718,114 - QEN71_RS03225 0.46 -1.7 718,126 + QEN71_RS03225 0.62 -0.3 718,126 + QEN71_RS03225 0.62 -0.4 718,126 + QEN71_RS03225 0.62 -2.3 718,127 - QEN71_RS03225 0.64 +0.2 718,127 - QEN71_RS03225 0.64 +0.6 718,127 - QEN71_RS03225 0.64 -0.4 718,131 + QEN71_RS03225 0.69 +0.1 718,131 + QEN71_RS03225 0.69 +0.3 718,131 + QEN71_RS03225 0.69 -0.4 718,131 + QEN71_RS03225 0.69 +0.4 718,131 + QEN71_RS03225 0.69 +0.0 718,132 - QEN71_RS03225 0.70 -1.2 718,132 - QEN71_RS03225 0.70 -0.4 718,132 - QEN71_RS03225 0.70 +0.1 718,132 - QEN71_RS03225 0.70 +2.7 718,132 - QEN71_RS03225 0.70 -0.7 718,134 + QEN71_RS03225 0.73 +0.2 718,134 + QEN71_RS03225 0.73 +0.7 718,134 + QEN71_RS03225 0.73 +1.0 718,134 + QEN71_RS03225 0.73 -1.2 718,134 + QEN71_RS03225 0.73 -1.7 718,134 + QEN71_RS03225 0.73 -0.0 718,134 + QEN71_RS03225 0.73 -1.8 718,134 + QEN71_RS03225 0.73 -0.2 718,135 - QEN71_RS03225 0.74 +1.6 718,135 - QEN71_RS03225 0.74 -2.5 718,135 - QEN71_RS03225 0.74 +1.0 718,135 - QEN71_RS03225 0.74 -0.0 718,135 - QEN71_RS03225 0.74 -0.9 718,135 - QEN71_RS03225 0.74 +0.1 718,135 - QEN71_RS03225 0.74 +1.2 718,140 + QEN71_RS03225 0.81 -0.6 718,141 - QEN71_RS03225 0.82 +1.2 718,141 - QEN71_RS03225 0.82 +0.5 718,142 + QEN71_RS03225 0.84 -0.6 718,142 + QEN71_RS03225 0.84 -0.6 718,142 + QEN71_RS03225 0.84 -0.3 718,142 + QEN71_RS03225 0.84 -0.1 718,142 + QEN71_RS03225 0.84 -1.0 718,142 + QEN71_RS03225 0.84 +0.5 718,142 + QEN71_RS03225 0.84 +1.0 718,142 + QEN71_RS03225 0.84 +0.6 718,142 + QEN71_RS03225 0.84 -1.0 718,142 + QEN71_RS03225 0.84 +0.2 718,142 + QEN71_RS03225 0.84 -1.8 718,143 - QEN71_RS03225 0.85 +0.3 718,143 - QEN71_RS03225 0.85 -0.1 718,143 - QEN71_RS03225 0.85 -1.0 718,143 - QEN71_RS03225 0.85 -0.2 718,143 - QEN71_RS03225 0.85 +0.6 718,143 - QEN71_RS03225 0.85 +0.8 718,143 - QEN71_RS03225 0.85 +0.1 718,143 - QEN71_RS03225 0.85 +0.1 718,143 - QEN71_RS03225 0.85 +0.4 718,250 + -1.0 718,250 + -0.5 718,250 + -0.5 718,250 + -0.5 718,251 - -0.0 718,406 + QEN71_RS03230 0.27 -1.0 718,406 + QEN71_RS03230 0.27 -0.1 718,583 + QEN71_RS03230 0.59 +0.2 718,583 + QEN71_RS03230 0.59 -0.0 718,583 + QEN71_RS03230 0.59 -1.3 718,583 + QEN71_RS03230 0.59 -0.6 718,583 + QEN71_RS03230 0.59 -2.2 718,583 + QEN71_RS03230 0.59 -0.0 718,583 + QEN71_RS03230 0.59 -0.6 718,584 - QEN71_RS03230 0.59 +0.8 718,584 - QEN71_RS03230 0.59 +0.5 718,584 - QEN71_RS03230 0.59 +1.6 718,584 - QEN71_RS03230 0.59 -2.3 718,584 - QEN71_RS03230 0.59 -0.0 718,639 - QEN71_RS03230 0.69 -0.3 718,712 + QEN71_RS03230 0.82 -1.7 718,712 + QEN71_RS03230 0.82 -3.0 718,712 + QEN71_RS03230 0.82 -1.3 718,712 + QEN71_RS03230 0.82 -0.1 718,712 + QEN71_RS03230 0.82 -0.8 718,713 - QEN71_RS03230 0.82 -0.3 718,713 - QEN71_RS03230 0.82 -0.3 718,713 - QEN71_RS03230 0.82 -1.5 718,713 - QEN71_RS03230 0.82 -1.6 718,782 + -2.1 718,782 + -1.2 718,782 + -0.3 718,782 + -0.7 718,783 - -1.1 719,005 + QEN71_RS03235 0.17 -0.6 719,005 + QEN71_RS03235 0.17 +1.1 719,005 + QEN71_RS03235 0.17 -0.8 719,005 + QEN71_RS03235 0.17 -0.1 719,006 - QEN71_RS03235 0.17 -0.3 719,006 - QEN71_RS03235 0.17 -1.0 719,006 - QEN71_RS03235 0.17 +0.4 719,006 - QEN71_RS03235 0.17 +0.5 719,056 + QEN71_RS03235 0.33 -1.5 719,056 + QEN71_RS03235 0.33 +0.2 719,056 + QEN71_RS03235 0.33 -0.8 719,057 - QEN71_RS03235 0.33 -0.0 719,209 + QEN71_RS03235 0.81 -0.3 719,209 + QEN71_RS03235 0.81 -0.6 719,209 + QEN71_RS03235 0.81 -0.1 719,210 - QEN71_RS03235 0.81 -1.1 719,210 - QEN71_RS03235 0.81 -0.5 719,210 - QEN71_RS03235 0.81 +0.1 719,210 - QEN71_RS03235 0.81 -0.0 719,210 - QEN71_RS03235 0.81 +1.2 719,210 - QEN71_RS03235 0.81 +1.3 719,212 + QEN71_RS03235 0.82 -0.3 719,212 + QEN71_RS03235 0.82 +0.7 719,212 + QEN71_RS03235 0.82 -1.3 719,212 + QEN71_RS03235 0.82 -2.7 719,213 - QEN71_RS03235 0.82 -0.2 719,213 - QEN71_RS03235 0.82 -0.6 719,213 - QEN71_RS03235 0.82 -1.0 719,213 - QEN71_RS03235 0.82 -0.1 719,213 - QEN71_RS03235 0.82 -0.4 719,213 - QEN71_RS03235 0.82 +0.4 719,213 - QEN71_RS03235 0.82 -0.9 719,213 - QEN71_RS03235 0.82 +0.8 719,213 - QEN71_RS03235 0.82 -0.8 719,397 + +0.2 719,398 - -0.6 719,433 + -0.8 719,433 + -0.8 719,433 + -0.3 719,433 + +0.3 719,434 - +0.8 719,434 - +0.6 719,434 - -0.5 719,434 - -0.0 719,434 - +1.0 719,434 - +0.0 719,443 + +0.4 719,444 - -0.4 719,444 - -0.1
Or see this region's nucleotide sequence