Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS42750 and QEN71_RS42755 are separated by 166 nucleotides QEN71_RS42755 and QEN71_RS42760 are separated by 35 nucleotides QEN71_RS42760 and QEN71_RS42765 are separated by 16 nucleotides QEN71_RS42765 and QEN71_RS42770 are separated by 203 nucleotides
QEN71_RS42750: QEN71_RS42750 - tyrosine-type recombinase/integrase, at 30,669 to 31,307
_RS42750
QEN71_RS42755: QEN71_RS42755 - IS3 family transposase, at 31,474 to 31,557
_RS42755
QEN71_RS42760: QEN71_RS42760 - transposase domain-containing protein, at 31,593 to 31,799
_RS42760
QEN71_RS42765: QEN71_RS42765 - IS5 family transposase, at 31,816 to 32,628
_RS42765
QEN71_RS42770: QEN71_RS42770 - site-specific integrase, at 32,832 to 33,710
_RS42770
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1 at 30.821 kb on - strand, within QEN71_RS42750 at 30.911 kb on + strand, within QEN71_RS42750 at 30.911 kb on + strand, within QEN71_RS42750 at 30.911 kb on + strand, within QEN71_RS42750 at 30.912 kb on - strand, within QEN71_RS42750 at 30.990 kb on + strand, within QEN71_RS42750 at 31.019 kb on + strand, within QEN71_RS42750 at 31.020 kb on - strand, within QEN71_RS42750 at 31.020 kb on - strand, within QEN71_RS42750 at 31.020 kb on - strand, within QEN71_RS42750 at 31.020 kb on - strand, within QEN71_RS42750 at 31.165 kb on + strand, within QEN71_RS42750 at 31.187 kb on + strand, within QEN71_RS42750 at 31.296 kb on + strand at 31.409 kb on + strand at 31.429 kb on + strand at 31.429 kb on + strand at 31.429 kb on + strand at 31.493 kb on + strand, within QEN71_RS42755 at 31.530 kb on + strand, within QEN71_RS42755 at 31.531 kb on - strand, within QEN71_RS42755 at 31.531 kb on - strand, within QEN71_RS42755 at 31.544 kb on + strand, within QEN71_RS42755 at 31.544 kb on + strand, within QEN71_RS42755 at 31.545 kb on - strand, within QEN71_RS42755 at 31.545 kb on - strand, within QEN71_RS42755 at 31.561 kb on - strand at 31.561 kb on - strand at 31.561 kb on - strand at 31.805 kb on - strand at 31.808 kb on - strand at 31.832 kb on - strand at 32.662 kb on + strand at 32.769 kb on + strand at 32.991 kb on + strand, within QEN71_RS42770 at 32.992 kb on - strand, within QEN71_RS42770 at 33.409 kb on + strand, within QEN71_RS42770
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 30,821 - QEN71_RS42750 0.24 +0.6 30,911 + QEN71_RS42750 0.38 +0.9 30,911 + QEN71_RS42750 0.38 +0.5 30,911 + QEN71_RS42750 0.38 -0.6 30,912 - QEN71_RS42750 0.38 -0.2 30,990 + QEN71_RS42750 0.50 -0.2 31,019 + QEN71_RS42750 0.55 -0.6 31,020 - QEN71_RS42750 0.55 -0.9 31,020 - QEN71_RS42750 0.55 -0.1 31,020 - QEN71_RS42750 0.55 -0.5 31,020 - QEN71_RS42750 0.55 -0.1 31,165 + QEN71_RS42750 0.78 -0.2 31,187 + QEN71_RS42750 0.81 -0.2 31,296 + +1.0 31,409 + +0.2 31,429 + -1.2 31,429 + -0.8 31,429 + +0.2 31,493 + QEN71_RS42755 0.23 -0.7 31,530 + QEN71_RS42755 0.67 -1.0 31,531 - QEN71_RS42755 0.68 +0.3 31,531 - QEN71_RS42755 0.68 +0.3 31,544 + QEN71_RS42755 0.83 -0.0 31,544 + QEN71_RS42755 0.83 +0.3 31,545 - QEN71_RS42755 0.85 -2.3 31,545 - QEN71_RS42755 0.85 -0.5 31,561 - -0.5 31,561 - +0.8 31,561 - -0.7 31,805 - +0.5 31,808 - -1.4 31,832 - -0.2 32,662 + -1.5 32,769 + -0.4 32,991 + QEN71_RS42770 0.18 -0.2 32,992 - QEN71_RS42770 0.18 -0.8 33,409 + QEN71_RS42770 0.66 -2.5
Or see this region's nucleotide sequence