Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS41975 and QEN71_RS41980 are separated by 275 nucleotides QEN71_RS41980 and QEN71_RS41985 are separated by 152 nucleotides QEN71_RS41985 and QEN71_RS41990 overlap by 2 nucleotides QEN71_RS41990 and QEN71_RS41995 are separated by 510 nucleotides
QEN71_RS41975: QEN71_RS41975 - IS5 family transposase, at 476,736 to 477,547
_RS41975
QEN71_RS41980: QEN71_RS41980 - MFS transporter, at 477,823 to 478,341
_RS41980
QEN71_RS41985: QEN71_RS41985 - IS5/IS1182 family transposase, at 478,494 to 478,616
_RS41985
QEN71_RS41990: QEN71_RS41990 - IS6 family transposase, at 478,615 to 478,716
_RS41990
QEN71_RS41995: QEN71_RS41995 - hemerythrin domain-containing protein, at 479,227 to 479,787
_RS41995
Position (kb) 477
478
479 Strain fitness (log2 ratio) -2
-1
0
1
2 at 476.845 kb on - strand, within QEN71_RS41975 at 476.845 kb on - strand, within QEN71_RS41975 at 477.545 kb on + strand at 477.545 kb on + strand at 477.555 kb on + strand at 477.655 kb on + strand at 477.695 kb on - strand at 477.695 kb on - strand at 477.697 kb on + strand at 477.949 kb on - strand, within QEN71_RS41980 at 477.967 kb on + strand, within QEN71_RS41980 at 477.967 kb on + strand, within QEN71_RS41980 at 477.968 kb on - strand, within QEN71_RS41980 at 478.009 kb on + strand, within QEN71_RS41980 at 478.085 kb on + strand, within QEN71_RS41980 at 478.085 kb on + strand, within QEN71_RS41980 at 478.086 kb on - strand, within QEN71_RS41980 at 478.086 kb on - strand, within QEN71_RS41980 at 478.148 kb on - strand, within QEN71_RS41980 at 478.262 kb on - strand, within QEN71_RS41980 at 478.315 kb on + strand at 478.315 kb on + strand at 478.349 kb on + strand at 478.437 kb on + strand at 478.438 kb on - strand at 478.447 kb on - strand at 478.582 kb on + strand, within QEN71_RS41985 at 478.582 kb on + strand, within QEN71_RS41985 at 478.582 kb on + strand, within QEN71_RS41985 at 478.582 kb on + strand, within QEN71_RS41985 at 478.583 kb on - strand, within QEN71_RS41985 at 478.583 kb on - strand, within QEN71_RS41985 at 478.610 kb on + strand at 478.611 kb on - strand at 478.614 kb on + strand at 478.615 kb on - strand at 478.657 kb on - strand, within QEN71_RS41990 at 478.690 kb on - strand, within QEN71_RS41990 at 478.691 kb on + strand, within QEN71_RS41990 at 478.730 kb on - strand at 478.730 kb on - strand at 478.747 kb on - strand at 479.003 kb on + strand at 479.003 kb on + strand at 479.003 kb on + strand at 479.129 kb on + strand at 479.235 kb on - strand at 479.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 476,845 - QEN71_RS41975 0.13 +1.9 476,845 - QEN71_RS41975 0.13 -0.1 477,545 + -0.2 477,545 + -0.6 477,555 + +0.1 477,655 + +1.0 477,695 - +0.1 477,695 - -0.1 477,697 + -0.2 477,949 - QEN71_RS41980 0.24 +0.1 477,967 + QEN71_RS41980 0.28 +0.2 477,967 + QEN71_RS41980 0.28 +0.8 477,968 - QEN71_RS41980 0.28 +0.1 478,009 + QEN71_RS41980 0.36 -1.8 478,085 + QEN71_RS41980 0.50 -1.3 478,085 + QEN71_RS41980 0.50 +0.3 478,086 - QEN71_RS41980 0.51 +1.5 478,086 - QEN71_RS41980 0.51 -0.4 478,148 - QEN71_RS41980 0.63 +1.9 478,262 - QEN71_RS41980 0.85 -0.7 478,315 + +0.4 478,315 + +0.1 478,349 + -0.6 478,437 + -0.2 478,438 - -1.1 478,447 - +0.6 478,582 + QEN71_RS41985 0.72 +0.3 478,582 + QEN71_RS41985 0.72 -1.5 478,582 + QEN71_RS41985 0.72 -0.1 478,582 + QEN71_RS41985 0.72 -0.2 478,583 - QEN71_RS41985 0.72 +0.5 478,583 - QEN71_RS41985 0.72 +0.3 478,610 + -0.7 478,611 - +0.1 478,614 + -0.9 478,615 - -0.2 478,657 - QEN71_RS41990 0.41 -0.9 478,690 - QEN71_RS41990 0.74 +1.1 478,691 + QEN71_RS41990 0.75 -0.6 478,730 - -0.2 478,730 - +0.5 478,747 - +0.3 479,003 + +0.4 479,003 + -0.4 479,003 + -0.3 479,129 + +0.1 479,235 - -1.0 479,235 - -0.3
Or see this region's nucleotide sequence