Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS30210 and QEN71_RS30215 are separated by 216 nucleotides QEN71_RS30215 and QEN71_RS30220 are separated by 83 nucleotides
QEN71_RS30210: QEN71_RS30210 - SDR family oxidoreductase, at 126,595 to 127,341
_RS30210
QEN71_RS30215: QEN71_RS30215 - LysR family transcriptional regulator, at 127,558 to 128,442
_RS30215
QEN71_RS30220: QEN71_RS30220 - hypothetical protein, at 128,526 to 129,053
_RS30220
Position (kb) 127
128
129 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 126.563 kb on + strand at 126.563 kb on + strand at 126.563 kb on + strand at 126.563 kb on + strand at 126.564 kb on - strand at 126.564 kb on - strand at 126.564 kb on - strand at 126.564 kb on - strand at 126.564 kb on - strand at 126.564 kb on - strand at 126.564 kb on - strand at 126.585 kb on + strand at 126.585 kb on + strand at 126.585 kb on + strand at 126.585 kb on + strand at 126.585 kb on + strand at 126.585 kb on + strand at 126.586 kb on - strand at 126.879 kb on - strand, within QEN71_RS30210 at 126.949 kb on + strand, within QEN71_RS30210 at 126.949 kb on + strand, within QEN71_RS30210 at 126.949 kb on + strand, within QEN71_RS30210 at 126.950 kb on - strand, within QEN71_RS30210 at 126.950 kb on - strand, within QEN71_RS30210 at 126.950 kb on - strand, within QEN71_RS30210 at 126.950 kb on - strand, within QEN71_RS30210 at 127.232 kb on + strand, within QEN71_RS30210 at 127.232 kb on + strand, within QEN71_RS30210 at 127.233 kb on - strand, within QEN71_RS30210 at 127.233 kb on - strand, within QEN71_RS30210 at 127.233 kb on - strand, within QEN71_RS30210 at 127.233 kb on - strand, within QEN71_RS30210 at 127.394 kb on - strand at 127.451 kb on - strand at 127.451 kb on - strand at 127.455 kb on + strand at 127.489 kb on - strand at 127.565 kb on + strand at 127.565 kb on + strand at 127.565 kb on + strand at 127.566 kb on - strand at 127.566 kb on - strand at 127.566 kb on - strand at 127.566 kb on - strand at 127.566 kb on - strand at 127.566 kb on - strand at 127.566 kb on - strand at 127.579 kb on + strand at 127.579 kb on + strand at 127.942 kb on + strand, within QEN71_RS30215 at 127.942 kb on + strand, within QEN71_RS30215 at 127.943 kb on - strand, within QEN71_RS30215 at 128.368 kb on + strand at 128.458 kb on + strand at 128.524 kb on + strand at 128.525 kb on - strand at 128.525 kb on - strand at 128.585 kb on + strand, within QEN71_RS30220 at 128.604 kb on + strand, within QEN71_RS30220 at 128.604 kb on + strand, within QEN71_RS30220 at 128.605 kb on - strand, within QEN71_RS30220 at 129.115 kb on - strand at 129.115 kb on - strand at 129.129 kb on + strand at 129.130 kb on - strand at 129.130 kb on - strand at 129.130 kb on - strand at 129.145 kb on - strand at 129.145 kb on - strand at 129.159 kb on + strand at 129.159 kb on + strand at 129.160 kb on - strand at 129.160 kb on - strand at 129.160 kb on - strand at 129.160 kb on - strand at 129.174 kb on + strand at 129.189 kb on + strand at 129.204 kb on + strand at 129.235 kb on - strand at 129.294 kb on + strand at 129.309 kb on + strand at 129.310 kb on - strand at 129.417 kb on + strand at 129.418 kb on - strand at 129.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 126,563 + +0.8 126,563 + +0.3 126,563 + +0.0 126,563 + -0.4 126,564 - +1.1 126,564 - +0.1 126,564 - +0.4 126,564 - +0.3 126,564 - +0.1 126,564 - +1.1 126,564 - +2.0 126,585 + -1.8 126,585 + -3.0 126,585 + -1.2 126,585 + +0.3 126,585 + -0.2 126,585 + -0.3 126,586 - +0.1 126,879 - QEN71_RS30210 0.38 -0.0 126,949 + QEN71_RS30210 0.47 -1.0 126,949 + QEN71_RS30210 0.47 +0.3 126,949 + QEN71_RS30210 0.47 +0.3 126,950 - QEN71_RS30210 0.48 +0.4 126,950 - QEN71_RS30210 0.48 -1.5 126,950 - QEN71_RS30210 0.48 +1.2 126,950 - QEN71_RS30210 0.48 +0.7 127,232 + QEN71_RS30210 0.85 -0.7 127,232 + QEN71_RS30210 0.85 -0.4 127,233 - QEN71_RS30210 0.85 +0.2 127,233 - QEN71_RS30210 0.85 -0.2 127,233 - QEN71_RS30210 0.85 -1.9 127,233 - QEN71_RS30210 0.85 +0.1 127,394 - +2.1 127,451 - -0.2 127,451 - +0.9 127,455 + +1.0 127,489 - +1.5 127,565 + -0.5 127,565 + -0.6 127,565 + -1.6 127,566 - -0.1 127,566 - -0.1 127,566 - -0.7 127,566 - -0.3 127,566 - +0.6 127,566 - +0.1 127,566 - +1.7 127,579 + -0.4 127,579 + -0.1 127,942 + QEN71_RS30215 0.43 -0.9 127,942 + QEN71_RS30215 0.43 -0.2 127,943 - QEN71_RS30215 0.44 +0.7 128,368 + -1.5 128,458 + +0.1 128,524 + -1.2 128,525 - -0.7 128,525 - -0.1 128,585 + QEN71_RS30220 0.11 -0.8 128,604 + QEN71_RS30220 0.15 -1.4 128,604 + QEN71_RS30220 0.15 -1.1 128,605 - QEN71_RS30220 0.15 +0.2 129,115 - -1.3 129,115 - -2.2 129,129 + -0.2 129,130 - -0.5 129,130 - -0.8 129,130 - -0.8 129,145 - +1.0 129,145 - -0.5 129,159 + +1.8 129,159 + -0.3 129,160 - +1.1 129,160 - +1.1 129,160 - -0.1 129,160 - +0.6 129,174 + -0.0 129,189 + -1.6 129,204 + +3.1 129,235 - +0.8 129,294 + +0.9 129,309 + -0.6 129,310 - +0.3 129,417 + -1.5 129,418 - -0.4 129,418 - -1.2
Or see this region's nucleotide sequence