Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS09960 and QEN71_RS09965 are separated by 140 nucleotides QEN71_RS09965 and QEN71_RS09970 are separated by 142 nucleotides
QEN71_RS09960: QEN71_RS09960 - DUF4410 domain-containing protein, at 2,217,472 to 2,218,239
_RS09960
QEN71_RS09965: QEN71_RS09965 - hypothetical protein, at 2,218,380 to 2,218,709
_RS09965
QEN71_RS09970: QEN71_RS09970 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,218,852 to 2,220,000
_RS09970
Position (kb)
2218
2219 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2217.469 kb on - strand at 2217.473 kb on + strand at 2217.474 kb on - strand at 2217.474 kb on - strand at 2217.474 kb on - strand at 2217.681 kb on + strand, within QEN71_RS09960 at 2217.681 kb on + strand, within QEN71_RS09960 at 2217.682 kb on - strand, within QEN71_RS09960 at 2217.682 kb on - strand, within QEN71_RS09960 at 2217.682 kb on - strand, within QEN71_RS09960 at 2217.682 kb on - strand, within QEN71_RS09960 at 2217.746 kb on + strand, within QEN71_RS09960 at 2217.747 kb on - strand, within QEN71_RS09960 at 2217.747 kb on - strand, within QEN71_RS09960 at 2217.747 kb on - strand, within QEN71_RS09960 at 2218.076 kb on + strand, within QEN71_RS09960 at 2218.077 kb on - strand, within QEN71_RS09960 at 2218.077 kb on - strand, within QEN71_RS09960 at 2218.083 kb on - strand, within QEN71_RS09960 at 2218.356 kb on - strand at 2218.356 kb on - strand at 2218.380 kb on - strand at 2218.549 kb on + strand, within QEN71_RS09965 at 2218.640 kb on + strand, within QEN71_RS09965 at 2218.640 kb on + strand, within QEN71_RS09965 at 2218.640 kb on + strand, within QEN71_RS09965 at 2218.640 kb on + strand, within QEN71_RS09965 at 2218.641 kb on - strand, within QEN71_RS09965 at 2218.641 kb on - strand, within QEN71_RS09965 at 2218.641 kb on - strand, within QEN71_RS09965 at 2218.641 kb on - strand, within QEN71_RS09965 at 2218.641 kb on - strand, within QEN71_RS09965 at 2218.643 kb on - strand, within QEN71_RS09965 at 2218.715 kb on + strand at 2218.715 kb on + strand at 2218.715 kb on + strand at 2218.715 kb on + strand at 2218.715 kb on + strand at 2218.716 kb on - strand at 2218.806 kb on + strand at 2218.807 kb on - strand at 2218.807 kb on - strand at 2218.808 kb on + strand at 2218.808 kb on + strand at 2218.809 kb on - strand at 2218.809 kb on - strand at 2218.809 kb on - strand at 2219.464 kb on + strand, within QEN71_RS09970 at 2219.465 kb on - strand, within QEN71_RS09970 at 2219.465 kb on - strand, within QEN71_RS09970 at 2219.608 kb on + strand, within QEN71_RS09970 at 2219.608 kb on + strand, within QEN71_RS09970
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 2,217,469 - +0.2 2,217,473 + -0.7 2,217,474 - -2.2 2,217,474 - -1.0 2,217,474 - -0.4 2,217,681 + QEN71_RS09960 0.27 -0.2 2,217,681 + QEN71_RS09960 0.27 +0.6 2,217,682 - QEN71_RS09960 0.27 +0.7 2,217,682 - QEN71_RS09960 0.27 -0.4 2,217,682 - QEN71_RS09960 0.27 -0.1 2,217,682 - QEN71_RS09960 0.27 -0.1 2,217,746 + QEN71_RS09960 0.36 -0.6 2,217,747 - QEN71_RS09960 0.36 +0.1 2,217,747 - QEN71_RS09960 0.36 -0.9 2,217,747 - QEN71_RS09960 0.36 -0.6 2,218,076 + QEN71_RS09960 0.79 -0.8 2,218,077 - QEN71_RS09960 0.79 -0.5 2,218,077 - QEN71_RS09960 0.79 -0.2 2,218,083 - QEN71_RS09960 0.80 +0.8 2,218,356 - -2.4 2,218,356 - -0.7 2,218,380 - +1.9 2,218,549 + QEN71_RS09965 0.51 -0.7 2,218,640 + QEN71_RS09965 0.79 -0.1 2,218,640 + QEN71_RS09965 0.79 -0.1 2,218,640 + QEN71_RS09965 0.79 +0.0 2,218,640 + QEN71_RS09965 0.79 +0.7 2,218,641 - QEN71_RS09965 0.79 +0.4 2,218,641 - QEN71_RS09965 0.79 -0.8 2,218,641 - QEN71_RS09965 0.79 +0.6 2,218,641 - QEN71_RS09965 0.79 +0.5 2,218,641 - QEN71_RS09965 0.79 +0.4 2,218,643 - QEN71_RS09965 0.80 +0.9 2,218,715 + -0.0 2,218,715 + +2.2 2,218,715 + -0.8 2,218,715 + -0.4 2,218,715 + -2.7 2,218,716 - +0.2 2,218,806 + +0.1 2,218,807 - -0.8 2,218,807 - -0.8 2,218,808 + -2.0 2,218,808 + -0.3 2,218,809 - +0.1 2,218,809 - -0.5 2,218,809 - -0.2 2,219,464 + QEN71_RS09970 0.53 -0.8 2,219,465 - QEN71_RS09970 0.53 -0.0 2,219,465 - QEN71_RS09970 0.53 -0.1 2,219,608 + QEN71_RS09970 0.66 -0.1 2,219,608 + QEN71_RS09970 0.66 +1.0
Or see this region's nucleotide sequence