Experiment: LB_noSalt with Zinc 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS30180 and QEN71_RS30185 are separated by 28 nucleotides QEN71_RS30185 and QEN71_RS30190 are separated by 76 nucleotides QEN71_RS30190 and QEN71_RS30195 are separated by 224 nucleotides QEN71_RS30195 and QEN71_RS30200 are separated by 40 nucleotides QEN71_RS30200 and QEN71_RS30205 are separated by 9 nucleotides
QEN71_RS30180: QEN71_RS30180 - hypothetical protein, at 123,653 to 123,895
_RS30180
QEN71_RS30185: QEN71_RS30185 - pyrroloquinoline quinone biosynthesis peptide chaperone PqqD, at 123,924 to 124,205
_RS30185
QEN71_RS30190: QEN71_RS30190 - pyrroloquinoline quinone precursor peptide PqqA, at 124,282 to 124,356
_RS30190
QEN71_RS30195: QEN71_RS30195 - SDR family oxidoreductase, at 124,581 to 125,327
_RS30195
QEN71_RS30200: QEN71_RS30200 - SDR family oxidoreductase, at 125,368 to 126,111
_RS30200
QEN71_RS30205: QEN71_RS30205 - DUF427 domain-containing protein, at 126,121 to 126,519
_RS30205
Position (kb) 124
125
126 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 123.694 kb on + strand, within QEN71_RS30180 at 123.908 kb on + strand at 123.909 kb on - strand at 123.968 kb on - strand, within QEN71_RS30185 at 124.110 kb on + strand, within QEN71_RS30185 at 124.110 kb on + strand, within QEN71_RS30185 at 124.110 kb on + strand, within QEN71_RS30185 at 124.111 kb on - strand, within QEN71_RS30185 at 124.111 kb on - strand, within QEN71_RS30185 at 124.111 kb on - strand, within QEN71_RS30185 at 124.114 kb on + strand, within QEN71_RS30185 at 124.114 kb on + strand, within QEN71_RS30185 at 124.115 kb on - strand, within QEN71_RS30185 at 124.115 kb on - strand, within QEN71_RS30185 at 124.297 kb on - strand, within QEN71_RS30190 at 124.297 kb on - strand, within QEN71_RS30190 at 124.298 kb on + strand, within QEN71_RS30190 at 124.298 kb on + strand, within QEN71_RS30190 at 124.298 kb on + strand, within QEN71_RS30190 at 124.299 kb on - strand, within QEN71_RS30190 at 124.299 kb on - strand, within QEN71_RS30190 at 124.299 kb on - strand, within QEN71_RS30190 at 124.299 kb on - strand, within QEN71_RS30190 at 124.299 kb on - strand, within QEN71_RS30190 at 124.299 kb on - strand, within QEN71_RS30190 at 124.334 kb on + strand, within QEN71_RS30190 at 124.334 kb on + strand, within QEN71_RS30190 at 124.504 kb on - strand at 124.505 kb on - strand at 124.506 kb on + strand at 124.507 kb on - strand at 124.507 kb on - strand at 124.507 kb on - strand at 124.507 kb on - strand at 124.755 kb on - strand, within QEN71_RS30195 at 124.765 kb on - strand, within QEN71_RS30195 at 124.839 kb on - strand, within QEN71_RS30195 at 124.864 kb on + strand, within QEN71_RS30195 at 124.865 kb on - strand, within QEN71_RS30195 at 125.218 kb on + strand, within QEN71_RS30195 at 125.369 kb on + strand at 125.435 kb on + strand at 125.435 kb on + strand at 125.651 kb on + strand, within QEN71_RS30200 at 125.652 kb on - strand, within QEN71_RS30200 at 125.652 kb on - strand, within QEN71_RS30200 at 126.123 kb on - strand at 126.123 kb on - strand at 126.158 kb on + strand at 126.159 kb on - strand at 126.159 kb on - strand at 126.209 kb on + strand, within QEN71_RS30205 at 126.209 kb on + strand, within QEN71_RS30205 at 126.209 kb on + strand, within QEN71_RS30205 at 126.261 kb on - strand, within QEN71_RS30205 at 126.279 kb on - strand, within QEN71_RS30205 at 126.287 kb on + strand, within QEN71_RS30205 at 126.287 kb on + strand, within QEN71_RS30205 at 126.288 kb on - strand, within QEN71_RS30205
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Zinc 0.5 mM remove 123,694 + QEN71_RS30180 0.17 -1.6 123,908 + +0.0 123,909 - +0.1 123,968 - QEN71_RS30185 0.16 -1.5 124,110 + QEN71_RS30185 0.66 +1.8 124,110 + QEN71_RS30185 0.66 -0.3 124,110 + QEN71_RS30185 0.66 -0.5 124,111 - QEN71_RS30185 0.66 +0.6 124,111 - QEN71_RS30185 0.66 -0.5 124,111 - QEN71_RS30185 0.66 +0.5 124,114 + QEN71_RS30185 0.67 -1.0 124,114 + QEN71_RS30185 0.67 -0.5 124,115 - QEN71_RS30185 0.68 -0.7 124,115 - QEN71_RS30185 0.68 -1.4 124,297 - QEN71_RS30190 0.20 -0.3 124,297 - QEN71_RS30190 0.20 +2.1 124,298 + QEN71_RS30190 0.21 +0.8 124,298 + QEN71_RS30190 0.21 +0.1 124,298 + QEN71_RS30190 0.21 -0.9 124,299 - QEN71_RS30190 0.23 -0.0 124,299 - QEN71_RS30190 0.23 +1.3 124,299 - QEN71_RS30190 0.23 +0.5 124,299 - QEN71_RS30190 0.23 -0.3 124,299 - QEN71_RS30190 0.23 +0.2 124,299 - QEN71_RS30190 0.23 -0.3 124,334 + QEN71_RS30190 0.69 -1.1 124,334 + QEN71_RS30190 0.69 -0.4 124,504 - +1.1 124,505 - +1.1 124,506 + +0.8 124,507 - +2.1 124,507 - -1.0 124,507 - -1.3 124,507 - +0.2 124,755 - QEN71_RS30195 0.23 -0.0 124,765 - QEN71_RS30195 0.25 +0.7 124,839 - QEN71_RS30195 0.35 -3.3 124,864 + QEN71_RS30195 0.38 +0.6 124,865 - QEN71_RS30195 0.38 -1.8 125,218 + QEN71_RS30195 0.85 +0.0 125,369 + +0.8 125,435 + -0.6 125,435 + +0.1 125,651 + QEN71_RS30200 0.38 -0.2 125,652 - QEN71_RS30200 0.38 +0.4 125,652 - QEN71_RS30200 0.38 -1.4 126,123 - -1.5 126,123 - +0.5 126,158 + +0.1 126,159 - -0.8 126,159 - +0.0 126,209 + QEN71_RS30205 0.22 -0.2 126,209 + QEN71_RS30205 0.22 -0.8 126,209 + QEN71_RS30205 0.22 +0.2 126,261 - QEN71_RS30205 0.35 -0.6 126,279 - QEN71_RS30205 0.40 +0.8 126,287 + QEN71_RS30205 0.42 -0.8 126,287 + QEN71_RS30205 0.42 +0.8 126,288 - QEN71_RS30205 0.42 +0.9
Or see this region's nucleotide sequence