Experiment: LB_noSalt with Zinc 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS03005 and QEN71_RS03010 are separated by 108 nucleotides QEN71_RS03010 and QEN71_RS03015 are separated by 64 nucleotides QEN71_RS03015 and QEN71_RS03020 are separated by 73 nucleotides
QEN71_RS03005: QEN71_RS03005 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 669,782 to 672,082
_RS03005
QEN71_RS03010: QEN71_RS03010 - dUTP diphosphatase, at 672,191 to 672,637
_RS03010
QEN71_RS03015: QEN71_RS03015 - LLM class flavin-dependent oxidoreductase, at 672,702 to 673,706
_RS03015
QEN71_RS03020: QEN71_RS03020 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 673,780 to 675,003
_RS03020
Position (kb) 672
673
674 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 672.080 kb on + strand at 672.080 kb on + strand at 672.080 kb on + strand at 672.080 kb on + strand at 672.081 kb on - strand at 672.081 kb on - strand at 672.081 kb on - strand at 672.081 kb on - strand at 672.081 kb on - strand at 672.140 kb on + strand at 672.140 kb on + strand at 672.695 kb on - strand at 672.742 kb on + strand at 672.743 kb on - strand at 672.743 kb on - strand at 672.743 kb on - strand at 672.743 kb on - strand at 672.764 kb on - strand at 672.907 kb on + strand, within QEN71_RS03015 at 672.908 kb on - strand, within QEN71_RS03015 at 672.908 kb on - strand, within QEN71_RS03015 at 672.958 kb on + strand, within QEN71_RS03015 at 672.978 kb on + strand, within QEN71_RS03015 at 672.978 kb on + strand, within QEN71_RS03015 at 672.979 kb on - strand, within QEN71_RS03015 at 672.979 kb on - strand, within QEN71_RS03015 at 672.979 kb on - strand, within QEN71_RS03015 at 673.054 kb on + strand, within QEN71_RS03015 at 673.054 kb on + strand, within QEN71_RS03015 at 673.055 kb on - strand, within QEN71_RS03015 at 673.334 kb on - strand, within QEN71_RS03015 at 673.334 kb on - strand, within QEN71_RS03015 at 673.334 kb on - strand, within QEN71_RS03015 at 673.334 kb on - strand, within QEN71_RS03015 at 673.334 kb on - strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.462 kb on + strand, within QEN71_RS03015 at 673.463 kb on - strand, within QEN71_RS03015 at 673.463 kb on - strand, within QEN71_RS03015 at 673.463 kb on - strand, within QEN71_RS03015 at 673.463 kb on - strand, within QEN71_RS03015 at 673.463 kb on - strand, within QEN71_RS03015 at 673.463 kb on - strand, within QEN71_RS03015 at 673.465 kb on + strand, within QEN71_RS03015 at 673.465 kb on + strand, within QEN71_RS03015 at 673.466 kb on - strand, within QEN71_RS03015 at 673.561 kb on + strand, within QEN71_RS03015 at 673.562 kb on - strand, within QEN71_RS03015 at 673.562 kb on - strand, within QEN71_RS03015 at 673.562 kb on - strand, within QEN71_RS03015 at 673.562 kb on - strand, within QEN71_RS03015 at 673.588 kb on + strand, within QEN71_RS03015 at 673.588 kb on + strand, within QEN71_RS03015 at 673.658 kb on + strand at 673.658 kb on + strand at 673.658 kb on + strand at 673.658 kb on + strand at 673.658 kb on + strand at 673.658 kb on + strand at 673.658 kb on + strand at 673.659 kb on - strand at 673.659 kb on - strand at 673.659 kb on - strand at 673.659 kb on - strand at 673.723 kb on + strand at 673.723 kb on + strand at 673.724 kb on - strand at 673.724 kb on - strand at 673.724 kb on - strand at 673.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Zinc 0.5 mM remove 672,080 + -0.7 672,080 + -0.6 672,080 + -0.9 672,080 + -1.7 672,081 - +1.8 672,081 - +0.7 672,081 - +0.6 672,081 - -0.2 672,081 - -0.2 672,140 + +0.2 672,140 + +0.3 672,695 - -1.0 672,742 + -1.2 672,743 - -0.6 672,743 - -0.7 672,743 - -0.7 672,743 - -0.0 672,764 - +0.2 672,907 + QEN71_RS03015 0.20 -0.2 672,908 - QEN71_RS03015 0.20 -0.0 672,908 - QEN71_RS03015 0.20 +0.7 672,958 + QEN71_RS03015 0.25 +1.9 672,978 + QEN71_RS03015 0.27 -0.4 672,978 + QEN71_RS03015 0.27 -0.2 672,979 - QEN71_RS03015 0.28 +0.3 672,979 - QEN71_RS03015 0.28 -0.3 672,979 - QEN71_RS03015 0.28 -0.1 673,054 + QEN71_RS03015 0.35 +1.5 673,054 + QEN71_RS03015 0.35 -0.8 673,055 - QEN71_RS03015 0.35 -1.1 673,334 - QEN71_RS03015 0.63 -0.9 673,334 - QEN71_RS03015 0.63 -0.4 673,334 - QEN71_RS03015 0.63 -1.5 673,334 - QEN71_RS03015 0.63 -0.2 673,334 - QEN71_RS03015 0.63 -1.1 673,462 + QEN71_RS03015 0.76 -0.1 673,462 + QEN71_RS03015 0.76 +0.2 673,462 + QEN71_RS03015 0.76 -0.2 673,462 + QEN71_RS03015 0.76 -0.3 673,462 + QEN71_RS03015 0.76 -1.7 673,462 + QEN71_RS03015 0.76 -3.5 673,462 + QEN71_RS03015 0.76 -0.5 673,462 + QEN71_RS03015 0.76 +1.1 673,462 + QEN71_RS03015 0.76 +2.8 673,462 + QEN71_RS03015 0.76 -0.8 673,462 + QEN71_RS03015 0.76 -1.0 673,463 - QEN71_RS03015 0.76 +0.3 673,463 - QEN71_RS03015 0.76 -0.5 673,463 - QEN71_RS03015 0.76 +1.3 673,463 - QEN71_RS03015 0.76 -0.8 673,463 - QEN71_RS03015 0.76 +0.1 673,463 - QEN71_RS03015 0.76 +0.2 673,465 + QEN71_RS03015 0.76 -1.7 673,465 + QEN71_RS03015 0.76 -2.6 673,466 - QEN71_RS03015 0.76 -1.2 673,561 + QEN71_RS03015 0.85 +0.7 673,562 - QEN71_RS03015 0.86 -0.6 673,562 - QEN71_RS03015 0.86 -2.5 673,562 - QEN71_RS03015 0.86 -1.0 673,562 - QEN71_RS03015 0.86 +0.6 673,588 + QEN71_RS03015 0.88 -0.2 673,588 + QEN71_RS03015 0.88 +0.1 673,658 + +0.8 673,658 + -0.7 673,658 + +1.1 673,658 + -0.9 673,658 + +0.3 673,658 + -0.1 673,658 + +0.7 673,659 - -0.0 673,659 - +0.3 673,659 - +0.4 673,659 - -2.5 673,723 + +0.2 673,723 + -0.5 673,724 - -0.1 673,724 - -0.1 673,724 - -1.2 673,724 - -0.6
Or see this region's nucleotide sequence