Experiment: Mixed culture with Prochlorococcus marinus str. MED4; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00514 and MIT1002_00515 are separated by 33 nucleotides MIT1002_00515 and MIT1002_00516 are separated by 100 nucleotides MIT1002_00516 and MIT1002_00517 are separated by 271 nucleotides
MIT1002_00514: MIT1002_00514 - Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 557,598 to 559,109
_00514
MIT1002_00515: MIT1002_00515 - ATP-dependent RNA helicase RhlB, at 559,143 to 560,435
_00515
MIT1002_00516: MIT1002_00516 - Thioredoxin-1, at 560,536 to 560,862
_00516
MIT1002_00517: MIT1002_00517 - hypothetical protein, at 561,134 to 562,399
_00517
Position (kb) 559
560
561 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 558.143 kb on + strand, within MIT1002_00514 at 558.151 kb on - strand, within MIT1002_00514 at 558.151 kb on - strand, within MIT1002_00514 at 558.151 kb on - strand, within MIT1002_00514 at 558.151 kb on - strand, within MIT1002_00514 at 558.358 kb on - strand, within MIT1002_00514 at 558.403 kb on + strand, within MIT1002_00514 at 558.403 kb on + strand, within MIT1002_00514 at 558.403 kb on + strand, within MIT1002_00514 at 558.411 kb on - strand, within MIT1002_00514 at 558.411 kb on - strand, within MIT1002_00514 at 558.435 kb on + strand, within MIT1002_00514 at 558.435 kb on + strand, within MIT1002_00514 at 558.508 kb on - strand, within MIT1002_00514 at 558.605 kb on - strand, within MIT1002_00514 at 558.692 kb on - strand, within MIT1002_00514 at 558.705 kb on + strand, within MIT1002_00514 at 558.806 kb on - strand, within MIT1002_00514 at 558.868 kb on - strand, within MIT1002_00514 at 558.870 kb on - strand, within MIT1002_00514 at 558.910 kb on + strand, within MIT1002_00514 at 558.953 kb on - strand, within MIT1002_00514 at 559.002 kb on + strand at 559.002 kb on + strand at 559.010 kb on - strand at 559.077 kb on + strand at 559.096 kb on + strand at 559.097 kb on - strand at 559.097 kb on - strand at 559.106 kb on + strand at 559.114 kb on - strand at 559.142 kb on - strand at 559.142 kb on - strand at 559.146 kb on + strand at 559.149 kb on - strand at 559.156 kb on + strand at 559.156 kb on + strand at 559.161 kb on - strand at 559.276 kb on + strand, within MIT1002_00515 at 559.319 kb on + strand, within MIT1002_00515 at 559.327 kb on - strand, within MIT1002_00515 at 559.328 kb on - strand, within MIT1002_00515 at 559.385 kb on - strand, within MIT1002_00515 at 559.385 kb on - strand, within MIT1002_00515 at 559.385 kb on - strand, within MIT1002_00515 at 559.470 kb on + strand, within MIT1002_00515 at 559.535 kb on - strand, within MIT1002_00515 at 559.586 kb on - strand, within MIT1002_00515 at 559.678 kb on + strand, within MIT1002_00515 at 559.678 kb on + strand, within MIT1002_00515 at 559.686 kb on - strand, within MIT1002_00515 at 559.776 kb on - strand, within MIT1002_00515 at 559.782 kb on - strand, within MIT1002_00515 at 559.782 kb on - strand, within MIT1002_00515 at 559.782 kb on - strand, within MIT1002_00515 at 559.784 kb on + strand, within MIT1002_00515 at 559.792 kb on - strand, within MIT1002_00515 at 559.811 kb on - strand, within MIT1002_00515 at 559.829 kb on + strand, within MIT1002_00515 at 559.837 kb on - strand, within MIT1002_00515 at 559.843 kb on - strand, within MIT1002_00515 at 559.862 kb on - strand, within MIT1002_00515 at 559.955 kb on + strand, within MIT1002_00515 at 560.068 kb on + strand, within MIT1002_00515 at 560.069 kb on + strand, within MIT1002_00515 at 560.069 kb on - strand, within MIT1002_00515 at 560.101 kb on + strand, within MIT1002_00515 at 560.136 kb on + strand, within MIT1002_00515 at 560.157 kb on + strand, within MIT1002_00515 at 560.274 kb on + strand, within MIT1002_00515 at 560.295 kb on + strand, within MIT1002_00515 at 560.295 kb on + strand, within MIT1002_00515 at 560.303 kb on - strand, within MIT1002_00515 at 560.370 kb on + strand at 560.373 kb on + strand at 560.437 kb on + strand at 560.437 kb on + strand at 560.438 kb on - strand at 560.445 kb on - strand at 560.445 kb on - strand at 560.445 kb on - strand at 560.919 kb on + strand at 561.041 kb on + strand at 561.049 kb on - strand at 561.052 kb on + strand at 561.052 kb on + strand at 561.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture with Prochlorococcus marinus str. MED4; Experiment B, time point 5 remove 558,143 + MIT1002_00514 0.36 +0.9 558,151 - MIT1002_00514 0.37 +1.1 558,151 - MIT1002_00514 0.37 +0.1 558,151 - MIT1002_00514 0.37 -0.7 558,151 - MIT1002_00514 0.37 -0.3 558,358 - MIT1002_00514 0.50 -2.1 558,403 + MIT1002_00514 0.53 +0.7 558,403 + MIT1002_00514 0.53 +0.7 558,403 + MIT1002_00514 0.53 +0.1 558,411 - MIT1002_00514 0.54 -2.3 558,411 - MIT1002_00514 0.54 -0.1 558,435 + MIT1002_00514 0.55 -2.2 558,435 + MIT1002_00514 0.55 +3.5 558,508 - MIT1002_00514 0.60 -0.8 558,605 - MIT1002_00514 0.67 +0.8 558,692 - MIT1002_00514 0.72 +0.1 558,705 + MIT1002_00514 0.73 +0.6 558,806 - MIT1002_00514 0.80 +1.1 558,868 - MIT1002_00514 0.84 -0.5 558,870 - MIT1002_00514 0.84 -0.4 558,910 + MIT1002_00514 0.87 -0.5 558,953 - MIT1002_00514 0.90 -1.2 559,002 + +1.3 559,002 + +0.6 559,010 - -0.7 559,077 + -1.6 559,096 + +0.8 559,097 - -1.4 559,097 - -1.4 559,106 + +0.5 559,114 - -1.7 559,142 - -0.5 559,142 - +0.4 559,146 + +0.6 559,149 - +0.5 559,156 + +2.8 559,156 + -0.4 559,161 - +0.8 559,276 + MIT1002_00515 0.10 -1.7 559,319 + MIT1002_00515 0.14 -1.4 559,327 - MIT1002_00515 0.14 -2.2 559,328 - MIT1002_00515 0.14 -1.2 559,385 - MIT1002_00515 0.19 -0.1 559,385 - MIT1002_00515 0.19 +0.1 559,385 - MIT1002_00515 0.19 +0.0 559,470 + MIT1002_00515 0.25 -0.4 559,535 - MIT1002_00515 0.30 -0.6 559,586 - MIT1002_00515 0.34 -1.7 559,678 + MIT1002_00515 0.41 -2.4 559,678 + MIT1002_00515 0.41 +1.2 559,686 - MIT1002_00515 0.42 -0.1 559,776 - MIT1002_00515 0.49 -0.7 559,782 - MIT1002_00515 0.49 -1.3 559,782 - MIT1002_00515 0.49 -0.3 559,782 - MIT1002_00515 0.49 +0.5 559,784 + MIT1002_00515 0.50 -3.1 559,792 - MIT1002_00515 0.50 -1.0 559,811 - MIT1002_00515 0.52 +1.3 559,829 + MIT1002_00515 0.53 -0.2 559,837 - MIT1002_00515 0.54 +1.0 559,843 - MIT1002_00515 0.54 -0.7 559,862 - MIT1002_00515 0.56 -1.5 559,955 + MIT1002_00515 0.63 +1.5 560,068 + MIT1002_00515 0.72 -1.6 560,069 + MIT1002_00515 0.72 -0.8 560,069 - MIT1002_00515 0.72 +0.0 560,101 + MIT1002_00515 0.74 -0.1 560,136 + MIT1002_00515 0.77 -3.2 560,157 + MIT1002_00515 0.78 +2.3 560,274 + MIT1002_00515 0.87 -0.7 560,295 + MIT1002_00515 0.89 -1.0 560,295 + MIT1002_00515 0.89 -0.1 560,303 - MIT1002_00515 0.90 -2.0 560,370 + +0.8 560,373 + -1.0 560,437 + -0.6 560,437 + +0.5 560,438 - -1.6 560,445 - -1.3 560,445 - -0.3 560,445 - -2.0 560,919 + -0.6 561,041 + -0.6 561,049 - -0.1 561,052 + -0.8 561,052 + -2.1 561,052 + -1.7
Or see this region's nucleotide sequence