Experiment: LB_noSalt with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS40735 and QEN71_RS40740 are separated by 30 nucleotides QEN71_RS40740 and QEN71_RS40745 overlap by 1 nucleotides
QEN71_RS40735: QEN71_RS40735 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 207,164 to 208,411
_RS40735
QEN71_RS40740: QEN71_RS40740 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 208,442 to 209,602
_RS40740
QEN71_RS40745: QEN71_RS40745 - polysaccharide biosynthesis tyrosine autokinase, at 209,602 to 211,857
_RS40745
Position (kb) 208
209
210 Strain fitness (log2 ratio) -2
-1
0
1
2 at 207.519 kb on + strand, within QEN71_RS40735 at 207.519 kb on + strand, within QEN71_RS40735 at 207.519 kb on + strand, within QEN71_RS40735 at 207.520 kb on - strand, within QEN71_RS40735 at 207.520 kb on - strand, within QEN71_RS40735 at 207.520 kb on - strand, within QEN71_RS40735 at 207.746 kb on - strand, within QEN71_RS40735 at 207.749 kb on + strand, within QEN71_RS40735 at 207.824 kb on + strand, within QEN71_RS40735 at 207.825 kb on - strand, within QEN71_RS40735 at 208.084 kb on + strand, within QEN71_RS40735 at 208.084 kb on + strand, within QEN71_RS40735 at 208.122 kb on + strand, within QEN71_RS40735 at 208.123 kb on - strand, within QEN71_RS40735 at 208.123 kb on - strand, within QEN71_RS40735 at 208.159 kb on + strand, within QEN71_RS40735 at 208.160 kb on - strand, within QEN71_RS40735 at 208.160 kb on - strand, within QEN71_RS40735 at 208.396 kb on + strand at 208.396 kb on + strand at 208.396 kb on + strand at 208.396 kb on + strand at 208.397 kb on - strand at 208.397 kb on - strand at 208.474 kb on + strand at 208.475 kb on - strand at 208.477 kb on + strand at 208.477 kb on + strand at 208.549 kb on + strand at 208.629 kb on - strand, within QEN71_RS40740 at 208.756 kb on + strand, within QEN71_RS40740 at 208.804 kb on + strand, within QEN71_RS40740 at 208.804 kb on + strand, within QEN71_RS40740 at 208.805 kb on - strand, within QEN71_RS40740 at 208.866 kb on + strand, within QEN71_RS40740 at 209.080 kb on + strand, within QEN71_RS40740 at 209.094 kb on + strand, within QEN71_RS40740 at 209.094 kb on + strand, within QEN71_RS40740 at 209.094 kb on + strand, within QEN71_RS40740 at 209.094 kb on + strand, within QEN71_RS40740 at 209.095 kb on - strand, within QEN71_RS40740 at 209.096 kb on + strand, within QEN71_RS40740 at 209.097 kb on - strand, within QEN71_RS40740 at 209.171 kb on + strand, within QEN71_RS40740 at 209.172 kb on - strand, within QEN71_RS40740 at 209.269 kb on + strand, within QEN71_RS40740 at 209.271 kb on + strand, within QEN71_RS40740 at 209.271 kb on + strand, within QEN71_RS40740 at 209.272 kb on - strand, within QEN71_RS40740 at 209.274 kb on + strand, within QEN71_RS40740 at 209.274 kb on + strand, within QEN71_RS40740 at 209.275 kb on - strand, within QEN71_RS40740 at 209.355 kb on + strand, within QEN71_RS40740 at 209.356 kb on - strand, within QEN71_RS40740 at 209.417 kb on - strand, within QEN71_RS40740 at 209.576 kb on + strand at 209.576 kb on + strand at 209.681 kb on + strand at 209.688 kb on - strand at 209.721 kb on + strand at 209.869 kb on + strand, within QEN71_RS40745 at 209.870 kb on - strand, within QEN71_RS40745 at 209.900 kb on + strand, within QEN71_RS40745 at 209.900 kb on + strand, within QEN71_RS40745 at 209.901 kb on - strand, within QEN71_RS40745 at 209.916 kb on + strand, within QEN71_RS40745 at 209.916 kb on + strand, within QEN71_RS40745 at 209.916 kb on + strand, within QEN71_RS40745 at 209.916 kb on + strand, within QEN71_RS40745 at 209.993 kb on - strand, within QEN71_RS40745 at 210.164 kb on - strand, within QEN71_RS40745 at 210.264 kb on + strand, within QEN71_RS40745 at 210.265 kb on - strand, within QEN71_RS40745 at 210.360 kb on + strand, within QEN71_RS40745
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Cobalt chloride 0.25 mM remove 207,519 + QEN71_RS40735 0.28 +2.0 207,519 + QEN71_RS40735 0.28 -0.6 207,519 + QEN71_RS40735 0.28 -0.4 207,520 - QEN71_RS40735 0.29 -1.3 207,520 - QEN71_RS40735 0.29 -0.4 207,520 - QEN71_RS40735 0.29 +1.0 207,746 - QEN71_RS40735 0.47 -0.5 207,749 + QEN71_RS40735 0.47 -0.7 207,824 + QEN71_RS40735 0.53 -0.3 207,825 - QEN71_RS40735 0.53 -1.6 208,084 + QEN71_RS40735 0.74 -0.0 208,084 + QEN71_RS40735 0.74 +2.1 208,122 + QEN71_RS40735 0.77 -1.8 208,123 - QEN71_RS40735 0.77 -1.5 208,123 - QEN71_RS40735 0.77 -0.6 208,159 + QEN71_RS40735 0.80 -2.1 208,160 - QEN71_RS40735 0.80 -0.3 208,160 - QEN71_RS40735 0.80 -1.1 208,396 + -0.9 208,396 + -1.0 208,396 + +0.1 208,396 + -0.5 208,397 - +0.1 208,397 - -0.7 208,474 + -1.6 208,475 - -0.7 208,477 + +0.7 208,477 + -0.3 208,549 + +0.5 208,629 - QEN71_RS40740 0.16 +1.0 208,756 + QEN71_RS40740 0.27 -0.6 208,804 + QEN71_RS40740 0.31 +0.0 208,804 + QEN71_RS40740 0.31 -1.0 208,805 - QEN71_RS40740 0.31 +0.5 208,866 + QEN71_RS40740 0.37 -1.3 209,080 + QEN71_RS40740 0.55 -1.2 209,094 + QEN71_RS40740 0.56 +0.4 209,094 + QEN71_RS40740 0.56 +0.5 209,094 + QEN71_RS40740 0.56 -2.2 209,094 + QEN71_RS40740 0.56 +2.4 209,095 - QEN71_RS40740 0.56 +1.6 209,096 + QEN71_RS40740 0.56 +0.9 209,097 - QEN71_RS40740 0.56 -1.3 209,171 + QEN71_RS40740 0.63 +0.7 209,172 - QEN71_RS40740 0.63 -0.6 209,269 + QEN71_RS40740 0.71 +0.3 209,271 + QEN71_RS40740 0.71 +0.0 209,271 + QEN71_RS40740 0.71 -2.2 209,272 - QEN71_RS40740 0.71 -1.2 209,274 + QEN71_RS40740 0.72 -0.2 209,274 + QEN71_RS40740 0.72 +0.6 209,275 - QEN71_RS40740 0.72 -0.5 209,355 + QEN71_RS40740 0.79 -0.7 209,356 - QEN71_RS40740 0.79 +0.2 209,417 - QEN71_RS40740 0.84 +0.1 209,576 + -0.4 209,576 + +0.1 209,681 + +0.2 209,688 - +0.3 209,721 + -0.0 209,869 + QEN71_RS40745 0.12 -1.3 209,870 - QEN71_RS40745 0.12 +0.5 209,900 + QEN71_RS40745 0.13 +0.8 209,900 + QEN71_RS40745 0.13 -0.1 209,901 - QEN71_RS40745 0.13 -0.3 209,916 + QEN71_RS40745 0.14 -0.8 209,916 + QEN71_RS40745 0.14 -0.7 209,916 + QEN71_RS40745 0.14 -0.2 209,916 + QEN71_RS40745 0.14 -0.8 209,993 - QEN71_RS40745 0.17 -0.9 210,164 - QEN71_RS40745 0.25 -0.8 210,264 + QEN71_RS40745 0.29 +1.2 210,265 - QEN71_RS40745 0.29 -0.5 210,360 + QEN71_RS40745 0.34 -1.9
Or see this region's nucleotide sequence