Experiment: LB_noSalt with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS39885 and QEN71_RS39890 are separated by 158 nucleotides QEN71_RS39890 and QEN71_RS39895 are separated by 60 nucleotides QEN71_RS39895 and QEN71_RS39900 are separated by 64 nucleotides
QEN71_RS39885: QEN71_RS39885 - IS110 family transposase, at 22,242 to 23,380
_RS39885
QEN71_RS39890: QEN71_RS39890 - transposase, at 23,539 to 23,754
_RS39890
QEN71_RS39895: QEN71_RS39895 - transposase DNA-binding-containing protein, at 23,815 to 24,657
_RS39895
QEN71_RS39900: QEN71_RS39900 - transposase, at 24,722 to 25,231
_RS39900
Position (kb) 23
24 Strain fitness (log2 ratio) -1
0
1 at 23.485 kb on + strand at 23.501 kb on + strand at 23.519 kb on + strand at 23.519 kb on + strand at 23.520 kb on - strand at 23.520 kb on - strand at 23.673 kb on + strand, within QEN71_RS39890 at 23.674 kb on - strand, within QEN71_RS39890 at 23.674 kb on - strand, within QEN71_RS39890 at 23.674 kb on - strand, within QEN71_RS39890 at 23.677 kb on + strand, within QEN71_RS39890 at 23.678 kb on - strand, within QEN71_RS39890 at 23.678 kb on - strand, within QEN71_RS39890 at 23.678 kb on - strand, within QEN71_RS39890 at 23.678 kb on - strand, within QEN71_RS39890 at 23.767 kb on + strand at 23.767 kb on + strand at 23.768 kb on - strand at 24.719 kb on + strand at 24.719 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Cobalt chloride 0.25 mM remove 23,485 + -0.2 23,501 + +0.4 23,519 + +0.3 23,519 + -0.4 23,520 - -0.0 23,520 - -0.7 23,673 + QEN71_RS39890 0.62 -1.0 23,674 - QEN71_RS39890 0.62 -0.3 23,674 - QEN71_RS39890 0.62 +0.2 23,674 - QEN71_RS39890 0.62 -0.6 23,677 + QEN71_RS39890 0.64 -0.8 23,678 - QEN71_RS39890 0.64 -0.1 23,678 - QEN71_RS39890 0.64 -0.9 23,678 - QEN71_RS39890 0.64 -0.2 23,678 - QEN71_RS39890 0.64 -1.3 23,767 + -1.4 23,767 + +0.6 23,768 - -0.1 24,719 + -0.5 24,719 + -0.9
Or see this region's nucleotide sequence