Experiment: Mixed culture with Prochlorococcus marinus str. MED4; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00405 and MIT1002_00406 are separated by 210 nucleotides MIT1002_00406 and MIT1002_00407 are separated by 194 nucleotides
MIT1002_00405: MIT1002_00405 - 2'-5'-RNA ligase, at 434,796 to 435,449
_00405
MIT1002_00406: MIT1002_00406 - ATP-dependent RNA helicase HrpB, at 435,660 to 438,302
_00406
MIT1002_00407: MIT1002_00407 - Nickel uptake substrate-specific transmembrane region, at 438,497 to 439,321
_00407
Position (kb) 435
436
437
438
439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 434.664 kb on + strand at 434.690 kb on - strand at 434.690 kb on - strand at 434.691 kb on - strand at 434.691 kb on - strand at 434.719 kb on + strand at 434.725 kb on + strand at 434.733 kb on - strand at 434.771 kb on + strand at 434.781 kb on + strand at 434.789 kb on - strand at 434.822 kb on + strand at 434.836 kb on - strand at 434.854 kb on + strand at 434.854 kb on + strand at 434.862 kb on - strand, within MIT1002_00405 at 434.872 kb on + strand, within MIT1002_00405 at 434.872 kb on + strand, within MIT1002_00405 at 434.872 kb on + strand, within MIT1002_00405 at 434.872 kb on + strand, within MIT1002_00405 at 434.880 kb on - strand, within MIT1002_00405 at 434.884 kb on - strand, within MIT1002_00405 at 434.884 kb on - strand, within MIT1002_00405 at 434.885 kb on - strand, within MIT1002_00405 at 434.933 kb on + strand, within MIT1002_00405 at 434.939 kb on + strand, within MIT1002_00405 at 434.941 kb on - strand, within MIT1002_00405 at 434.954 kb on + strand, within MIT1002_00405 at 435.009 kb on - strand, within MIT1002_00405 at 435.023 kb on - strand, within MIT1002_00405 at 435.080 kb on + strand, within MIT1002_00405 at 435.140 kb on + strand, within MIT1002_00405 at 435.146 kb on + strand, within MIT1002_00405 at 435.165 kb on + strand, within MIT1002_00405 at 435.189 kb on + strand, within MIT1002_00405 at 435.191 kb on + strand, within MIT1002_00405 at 435.231 kb on + strand, within MIT1002_00405 at 435.265 kb on + strand, within MIT1002_00405 at 435.265 kb on + strand, within MIT1002_00405 at 435.267 kb on + strand, within MIT1002_00405 at 435.273 kb on - strand, within MIT1002_00405 at 435.273 kb on - strand, within MIT1002_00405 at 435.273 kb on - strand, within MIT1002_00405 at 435.273 kb on - strand, within MIT1002_00405 at 435.273 kb on - strand, within MIT1002_00405 at 435.367 kb on + strand, within MIT1002_00405 at 435.433 kb on - strand at 435.433 kb on - strand at 435.451 kb on + strand at 435.451 kb on + strand at 435.452 kb on - strand at 435.459 kb on - strand at 435.506 kb on + strand at 435.514 kb on - strand at 435.555 kb on + strand at 435.598 kb on - strand at 435.611 kb on - strand at 435.616 kb on - strand at 435.621 kb on + strand at 435.624 kb on - strand at 435.629 kb on - strand at 435.629 kb on - strand at 435.654 kb on + strand at 435.662 kb on - strand at 435.791 kb on + strand at 435.799 kb on - strand at 435.836 kb on - strand at 435.836 kb on - strand at 435.863 kb on + strand at 435.871 kb on - strand at 435.972 kb on + strand, within MIT1002_00406 at 435.980 kb on - strand, within MIT1002_00406 at 435.997 kb on + strand, within MIT1002_00406 at 436.005 kb on - strand, within MIT1002_00406 at 436.005 kb on - strand, within MIT1002_00406 at 436.051 kb on + strand, within MIT1002_00406 at 436.069 kb on + strand, within MIT1002_00406 at 436.093 kb on + strand, within MIT1002_00406 at 436.130 kb on - strand, within MIT1002_00406 at 436.137 kb on + strand, within MIT1002_00406 at 436.145 kb on - strand, within MIT1002_00406 at 436.145 kb on - strand, within MIT1002_00406 at 436.240 kb on + strand, within MIT1002_00406 at 436.288 kb on + strand, within MIT1002_00406 at 436.305 kb on + strand, within MIT1002_00406 at 436.360 kb on - strand, within MIT1002_00406 at 436.360 kb on - strand, within MIT1002_00406 at 436.415 kb on + strand, within MIT1002_00406 at 436.423 kb on - strand, within MIT1002_00406 at 436.423 kb on - strand, within MIT1002_00406 at 436.423 kb on - strand, within MIT1002_00406 at 436.430 kb on + strand, within MIT1002_00406 at 436.430 kb on + strand, within MIT1002_00406 at 436.438 kb on - strand, within MIT1002_00406 at 436.438 kb on - strand, within MIT1002_00406 at 436.438 kb on - strand, within MIT1002_00406 at 436.459 kb on + strand, within MIT1002_00406 at 436.523 kb on + strand, within MIT1002_00406 at 436.531 kb on + strand, within MIT1002_00406 at 436.567 kb on - strand, within MIT1002_00406 at 436.577 kb on - strand, within MIT1002_00406 at 436.683 kb on + strand, within MIT1002_00406 at 436.684 kb on - strand, within MIT1002_00406 at 436.773 kb on + strand, within MIT1002_00406 at 436.774 kb on + strand, within MIT1002_00406 at 436.781 kb on - strand, within MIT1002_00406 at 436.781 kb on - strand, within MIT1002_00406 at 436.781 kb on - strand, within MIT1002_00406 at 436.820 kb on + strand, within MIT1002_00406 at 436.828 kb on - strand, within MIT1002_00406 at 436.828 kb on - strand, within MIT1002_00406 at 436.828 kb on - strand, within MIT1002_00406 at 436.828 kb on - strand, within MIT1002_00406 at 436.865 kb on + strand, within MIT1002_00406 at 436.865 kb on + strand, within MIT1002_00406 at 436.865 kb on + strand, within MIT1002_00406 at 436.865 kb on + strand, within MIT1002_00406 at 436.865 kb on + strand, within MIT1002_00406 at 436.865 kb on + strand, within MIT1002_00406 at 436.873 kb on - strand, within MIT1002_00406 at 436.873 kb on - strand, within MIT1002_00406 at 436.873 kb on - strand, within MIT1002_00406 at 436.886 kb on + strand, within MIT1002_00406 at 436.896 kb on - strand, within MIT1002_00406 at 436.896 kb on - strand, within MIT1002_00406 at 436.896 kb on - strand, within MIT1002_00406 at 436.896 kb on - strand, within MIT1002_00406 at 436.898 kb on + strand, within MIT1002_00406 at 436.898 kb on + strand, within MIT1002_00406 at 436.906 kb on - strand, within MIT1002_00406 at 436.969 kb on + strand, within MIT1002_00406 at 436.979 kb on + strand, within MIT1002_00406 at 436.979 kb on + strand, within MIT1002_00406 at 437.087 kb on + strand, within MIT1002_00406 at 437.087 kb on + strand, within MIT1002_00406 at 437.087 kb on + strand, within MIT1002_00406 at 437.095 kb on - strand, within MIT1002_00406 at 437.124 kb on - strand, within MIT1002_00406 at 437.172 kb on + strand, within MIT1002_00406 at 437.180 kb on + strand, within MIT1002_00406 at 437.215 kb on + strand, within MIT1002_00406 at 437.215 kb on + strand, within MIT1002_00406 at 437.215 kb on + strand, within MIT1002_00406 at 437.215 kb on + strand, within MIT1002_00406 at 437.223 kb on - strand, within MIT1002_00406 at 437.263 kb on - strand, within MIT1002_00406 at 437.315 kb on + strand, within MIT1002_00406 at 437.315 kb on + strand, within MIT1002_00406 at 437.315 kb on + strand, within MIT1002_00406 at 437.315 kb on + strand, within MIT1002_00406 at 437.323 kb on - strand, within MIT1002_00406 at 437.323 kb on - strand, within MIT1002_00406 at 437.323 kb on - strand, within MIT1002_00406 at 437.323 kb on - strand, within MIT1002_00406 at 437.323 kb on - strand, within MIT1002_00406 at 437.323 kb on - strand, within MIT1002_00406 at 437.323 kb on - strand, within MIT1002_00406 at 437.359 kb on + strand, within MIT1002_00406 at 437.367 kb on - strand, within MIT1002_00406 at 437.367 kb on - strand, within MIT1002_00406 at 437.369 kb on - strand, within MIT1002_00406 at 437.373 kb on + strand, within MIT1002_00406 at 437.374 kb on - strand, within MIT1002_00406 at 437.374 kb on - strand, within MIT1002_00406 at 437.419 kb on + strand, within MIT1002_00406 at 437.514 kb on - strand, within MIT1002_00406 at 437.515 kb on + strand, within MIT1002_00406 at 437.547 kb on + strand, within MIT1002_00406 at 437.550 kb on - strand, within MIT1002_00406 at 437.557 kb on + strand, within MIT1002_00406 at 437.563 kb on - strand, within MIT1002_00406 at 437.565 kb on - strand, within MIT1002_00406 at 437.566 kb on + strand, within MIT1002_00406 at 437.703 kb on - strand, within MIT1002_00406 at 437.731 kb on - strand, within MIT1002_00406 at 437.739 kb on + strand, within MIT1002_00406 at 437.792 kb on + strand, within MIT1002_00406 at 437.849 kb on - strand, within MIT1002_00406 at 437.861 kb on - strand, within MIT1002_00406 at 437.988 kb on + strand, within MIT1002_00406 at 437.997 kb on + strand, within MIT1002_00406 at 437.997 kb on + strand, within MIT1002_00406 at 438.031 kb on - strand, within MIT1002_00406 at 438.079 kb on - strand at 438.090 kb on + strand at 438.090 kb on + strand at 438.137 kb on + strand at 438.146 kb on + strand at 438.154 kb on - strand at 438.196 kb on + strand at 438.235 kb on - strand at 438.299 kb on + strand at 438.321 kb on - strand at 438.428 kb on + strand at 438.466 kb on - strand at 438.483 kb on + strand at 438.622 kb on + strand, within MIT1002_00407 at 438.640 kb on + strand, within MIT1002_00407 at 438.653 kb on + strand, within MIT1002_00407 at 438.661 kb on - strand, within MIT1002_00407 at 438.661 kb on - strand, within MIT1002_00407 at 438.661 kb on - strand, within MIT1002_00407 at 438.670 kb on + strand, within MIT1002_00407 at 438.670 kb on + strand, within MIT1002_00407 at 438.678 kb on - strand, within MIT1002_00407 at 438.678 kb on - strand, within MIT1002_00407 at 438.678 kb on - strand, within MIT1002_00407 at 438.678 kb on - strand, within MIT1002_00407 at 438.678 kb on - strand, within MIT1002_00407 at 438.678 kb on - strand, within MIT1002_00407 at 438.699 kb on + strand, within MIT1002_00407 at 438.736 kb on + strand, within MIT1002_00407 at 438.737 kb on + strand, within MIT1002_00407 at 438.903 kb on - strand, within MIT1002_00407 at 438.932 kb on + strand, within MIT1002_00407 at 438.940 kb on + strand, within MIT1002_00407 at 438.965 kb on - strand, within MIT1002_00407 at 439.047 kb on - strand, within MIT1002_00407 at 439.102 kb on - strand, within MIT1002_00407 at 439.174 kb on + strand, within MIT1002_00407
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture with Prochlorococcus marinus str. MED4; Experiment C, time point 2 remove 434,664 + -1.5 434,690 - -0.8 434,690 - +1.0 434,691 - -1.1 434,691 - +0.8 434,719 + +0.2 434,725 + +0.9 434,733 - -0.7 434,771 + -0.7 434,781 + +0.9 434,789 - -0.3 434,822 + +0.3 434,836 - +0.9 434,854 + +0.8 434,854 + +1.5 434,862 - MIT1002_00405 0.10 -2.3 434,872 + MIT1002_00405 0.12 +1.3 434,872 + MIT1002_00405 0.12 -0.4 434,872 + MIT1002_00405 0.12 -1.6 434,872 + MIT1002_00405 0.12 -0.0 434,880 - MIT1002_00405 0.13 +0.6 434,884 - MIT1002_00405 0.13 -0.1 434,884 - MIT1002_00405 0.13 +0.5 434,885 - MIT1002_00405 0.14 -0.2 434,933 + MIT1002_00405 0.21 -0.6 434,939 + MIT1002_00405 0.22 +0.2 434,941 - MIT1002_00405 0.22 +0.1 434,954 + MIT1002_00405 0.24 -1.6 435,009 - MIT1002_00405 0.33 +0.1 435,023 - MIT1002_00405 0.35 +0.4 435,080 + MIT1002_00405 0.43 +0.3 435,140 + MIT1002_00405 0.53 -0.5 435,146 + MIT1002_00405 0.54 +0.0 435,165 + MIT1002_00405 0.56 +2.8 435,189 + MIT1002_00405 0.60 +0.0 435,191 + MIT1002_00405 0.60 +0.1 435,231 + MIT1002_00405 0.67 -0.9 435,265 + MIT1002_00405 0.72 +0.3 435,265 + MIT1002_00405 0.72 +0.3 435,267 + MIT1002_00405 0.72 -0.9 435,273 - MIT1002_00405 0.73 -0.4 435,273 - MIT1002_00405 0.73 -1.9 435,273 - MIT1002_00405 0.73 +0.2 435,273 - MIT1002_00405 0.73 +0.9 435,273 - MIT1002_00405 0.73 -0.3 435,367 + MIT1002_00405 0.87 -1.0 435,433 - -1.1 435,433 - +0.5 435,451 + -0.9 435,451 + +1.5 435,452 - +0.8 435,459 - +0.2 435,506 + +0.1 435,514 - +0.3 435,555 + +1.2 435,598 - +2.6 435,611 - -0.3 435,616 - +2.3 435,621 + +0.5 435,624 - -0.5 435,629 - +0.1 435,629 - +0.5 435,654 + +0.1 435,662 - -0.0 435,791 + +0.9 435,799 - -0.7 435,836 - -0.4 435,836 - -1.4 435,863 + -0.3 435,871 - -0.3 435,972 + MIT1002_00406 0.12 -0.8 435,980 - MIT1002_00406 0.12 +0.8 435,997 + MIT1002_00406 0.13 -2.4 436,005 - MIT1002_00406 0.13 +0.1 436,005 - MIT1002_00406 0.13 -0.8 436,051 + MIT1002_00406 0.15 -1.3 436,069 + MIT1002_00406 0.15 +0.6 436,093 + MIT1002_00406 0.16 +0.6 436,130 - MIT1002_00406 0.18 -1.6 436,137 + MIT1002_00406 0.18 +0.1 436,145 - MIT1002_00406 0.18 -1.0 436,145 - MIT1002_00406 0.18 -0.3 436,240 + MIT1002_00406 0.22 +1.0 436,288 + MIT1002_00406 0.24 +0.0 436,305 + MIT1002_00406 0.24 +0.9 436,360 - MIT1002_00406 0.26 +1.3 436,360 - MIT1002_00406 0.26 -0.3 436,415 + MIT1002_00406 0.29 +0.2 436,423 - MIT1002_00406 0.29 -0.4 436,423 - MIT1002_00406 0.29 -0.4 436,423 - MIT1002_00406 0.29 +1.0 436,430 + MIT1002_00406 0.29 +2.6 436,430 + MIT1002_00406 0.29 -0.3 436,438 - MIT1002_00406 0.29 +0.3 436,438 - MIT1002_00406 0.29 +0.5 436,438 - MIT1002_00406 0.29 +0.1 436,459 + MIT1002_00406 0.30 -0.7 436,523 + MIT1002_00406 0.33 +0.7 436,531 + MIT1002_00406 0.33 -2.6 436,567 - MIT1002_00406 0.34 +2.6 436,577 - MIT1002_00406 0.35 +0.2 436,683 + MIT1002_00406 0.39 +0.4 436,684 - MIT1002_00406 0.39 +0.4 436,773 + MIT1002_00406 0.42 -0.1 436,774 + MIT1002_00406 0.42 +0.7 436,781 - MIT1002_00406 0.42 -1.9 436,781 - MIT1002_00406 0.42 +0.6 436,781 - MIT1002_00406 0.42 -0.7 436,820 + MIT1002_00406 0.44 -0.4 436,828 - MIT1002_00406 0.44 +0.1 436,828 - MIT1002_00406 0.44 -1.9 436,828 - MIT1002_00406 0.44 -0.7 436,828 - MIT1002_00406 0.44 +0.8 436,865 + MIT1002_00406 0.46 +1.0 436,865 + MIT1002_00406 0.46 +0.0 436,865 + MIT1002_00406 0.46 -0.1 436,865 + MIT1002_00406 0.46 +0.0 436,865 + MIT1002_00406 0.46 -0.3 436,865 + MIT1002_00406 0.46 +0.3 436,873 - MIT1002_00406 0.46 -0.4 436,873 - MIT1002_00406 0.46 -0.5 436,873 - MIT1002_00406 0.46 +0.4 436,886 + MIT1002_00406 0.46 -1.2 436,896 - MIT1002_00406 0.47 +1.2 436,896 - MIT1002_00406 0.47 +0.9 436,896 - MIT1002_00406 0.47 -0.3 436,896 - MIT1002_00406 0.47 -0.8 436,898 + MIT1002_00406 0.47 +0.5 436,898 + MIT1002_00406 0.47 -0.7 436,906 - MIT1002_00406 0.47 +0.0 436,969 + MIT1002_00406 0.50 +1.2 436,979 + MIT1002_00406 0.50 -2.0 436,979 + MIT1002_00406 0.50 +0.2 437,087 + MIT1002_00406 0.54 -0.2 437,087 + MIT1002_00406 0.54 -0.1 437,087 + MIT1002_00406 0.54 +0.1 437,095 - MIT1002_00406 0.54 +0.3 437,124 - MIT1002_00406 0.55 -0.7 437,172 + MIT1002_00406 0.57 -0.3 437,180 + MIT1002_00406 0.58 +0.0 437,215 + MIT1002_00406 0.59 -0.7 437,215 + MIT1002_00406 0.59 -0.2 437,215 + MIT1002_00406 0.59 +1.0 437,215 + MIT1002_00406 0.59 -0.7 437,223 - MIT1002_00406 0.59 -0.3 437,263 - MIT1002_00406 0.61 -0.4 437,315 + MIT1002_00406 0.63 +0.6 437,315 + MIT1002_00406 0.63 +0.2 437,315 + MIT1002_00406 0.63 +1.1 437,315 + MIT1002_00406 0.63 -1.2 437,323 - MIT1002_00406 0.63 +0.6 437,323 - MIT1002_00406 0.63 +0.8 437,323 - MIT1002_00406 0.63 -1.9 437,323 - MIT1002_00406 0.63 -0.6 437,323 - MIT1002_00406 0.63 +0.2 437,323 - MIT1002_00406 0.63 +0.5 437,323 - MIT1002_00406 0.63 +1.1 437,359 + MIT1002_00406 0.64 -1.2 437,367 - MIT1002_00406 0.65 +0.3 437,367 - MIT1002_00406 0.65 -0.3 437,369 - MIT1002_00406 0.65 -0.1 437,373 + MIT1002_00406 0.65 -1.5 437,374 - MIT1002_00406 0.65 -0.7 437,374 - MIT1002_00406 0.65 -1.1 437,419 + MIT1002_00406 0.67 -0.0 437,514 - MIT1002_00406 0.70 +0.1 437,515 + MIT1002_00406 0.70 +0.7 437,547 + MIT1002_00406 0.71 +0.7 437,550 - MIT1002_00406 0.72 +0.3 437,557 + MIT1002_00406 0.72 +0.7 437,563 - MIT1002_00406 0.72 -0.0 437,565 - MIT1002_00406 0.72 -3.2 437,566 + MIT1002_00406 0.72 -1.4 437,703 - MIT1002_00406 0.77 +0.5 437,731 - MIT1002_00406 0.78 -2.2 437,739 + MIT1002_00406 0.79 +0.6 437,792 + MIT1002_00406 0.81 +0.8 437,849 - MIT1002_00406 0.83 -0.5 437,861 - MIT1002_00406 0.83 -1.6 437,988 + MIT1002_00406 0.88 -1.4 437,997 + MIT1002_00406 0.88 -1.0 437,997 + MIT1002_00406 0.88 -2.0 438,031 - MIT1002_00406 0.90 -2.0 438,079 - -1.0 438,090 + -2.9 438,090 + +1.1 438,137 + +0.3 438,146 + +0.5 438,154 - -0.2 438,196 + -0.6 438,235 - +0.2 438,299 + +0.2 438,321 - +0.8 438,428 + +0.6 438,466 - +0.4 438,483 + +0.3 438,622 + MIT1002_00407 0.15 +2.3 438,640 + MIT1002_00407 0.17 +0.4 438,653 + MIT1002_00407 0.19 -0.3 438,661 - MIT1002_00407 0.20 -0.3 438,661 - MIT1002_00407 0.20 -0.1 438,661 - MIT1002_00407 0.20 -0.1 438,670 + MIT1002_00407 0.21 +0.2 438,670 + MIT1002_00407 0.21 +0.2 438,678 - MIT1002_00407 0.22 +1.8 438,678 - MIT1002_00407 0.22 -0.3 438,678 - MIT1002_00407 0.22 +0.7 438,678 - MIT1002_00407 0.22 +0.9 438,678 - MIT1002_00407 0.22 +0.1 438,678 - MIT1002_00407 0.22 +0.0 438,699 + MIT1002_00407 0.24 +0.4 438,736 + MIT1002_00407 0.29 -0.7 438,737 + MIT1002_00407 0.29 +0.0 438,903 - MIT1002_00407 0.49 -0.0 438,932 + MIT1002_00407 0.53 +2.2 438,940 + MIT1002_00407 0.54 -0.3 438,965 - MIT1002_00407 0.57 +0.8 439,047 - MIT1002_00407 0.67 -0.0 439,102 - MIT1002_00407 0.73 +0.9 439,174 + MIT1002_00407 0.82 -0.6
Or see this region's nucleotide sequence