Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4143 and GFF4144 are separated by 92 nucleotides GFF4144 and GFF4145 overlap by 4 nucleotides GFF4145 and GFF4146 are separated by 15 nucleotides
GFF4143 - Chaperone SurA, at 151,510 to 152,418
GFF4143
GFF4144 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 152,511 to 153,575
GFF4144
GFF4145 - Ribosomal RNA small subunit methyltransferase A, at 153,572 to 154,450
GFF4145
GFF4146 - Methionine synthase, at 154,466 to 158,221
GFF4146
Position (kb)
152
153
154 Strain fitness (log2 ratio)
-1
0
1
2 at 151.520 kb on + strand at 151.520 kb on + strand at 152.455 kb on + strand at 152.456 kb on - strand at 152.456 kb on - strand at 152.694 kb on - strand, within GFF4144 at 152.910 kb on + strand, within GFF4144 at 152.910 kb on + strand, within GFF4144 at 152.911 kb on - strand, within GFF4144 at 152.911 kb on - strand, within GFF4144 at 152.911 kb on - strand, within GFF4144 at 152.911 kb on - strand, within GFF4144 at 153.051 kb on + strand, within GFF4144 at 153.287 kb on + strand, within GFF4144 at 153.319 kb on - strand, within GFF4144 at 153.339 kb on + strand, within GFF4144 at 153.339 kb on + strand, within GFF4144 at 153.339 kb on + strand, within GFF4144 at 153.339 kb on + strand, within GFF4144 at 153.340 kb on - strand, within GFF4144 at 153.340 kb on - strand, within GFF4144 at 153.340 kb on - strand, within GFF4144 at 153.340 kb on - strand, within GFF4144 at 153.848 kb on + strand, within GFF4145 at 153.848 kb on + strand, within GFF4145 at 153.848 kb on + strand, within GFF4145 at 153.947 kb on + strand, within GFF4145 at 153.947 kb on + strand, within GFF4145 at 154.080 kb on - strand, within GFF4145 at 154.448 kb on + strand at 154.449 kb on - strand at 154.449 kb on - strand at 154.467 kb on + strand at 154.468 kb on - strand at 154.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 151,520 + -1.6 151,520 + +0.7 152,455 + +0.8 152,456 - +0.6 152,456 - +1.1 152,694 - GFF4144 0.17 -0.1 152,910 + GFF4144 0.37 -1.2 152,910 + GFF4144 0.37 +0.5 152,911 - GFF4144 0.38 -0.7 152,911 - GFF4144 0.38 -0.5 152,911 - GFF4144 0.38 -0.1 152,911 - GFF4144 0.38 +1.3 153,051 + GFF4144 0.51 +1.8 153,287 + GFF4144 0.73 +0.1 153,319 - GFF4144 0.76 +0.9 153,339 + GFF4144 0.78 +0.1 153,339 + GFF4144 0.78 -0.4 153,339 + GFF4144 0.78 -0.8 153,339 + GFF4144 0.78 -1.2 153,340 - GFF4144 0.78 +0.8 153,340 - GFF4144 0.78 -0.5 153,340 - GFF4144 0.78 +0.5 153,340 - GFF4144 0.78 +1.2 153,848 + GFF4145 0.31 +0.4 153,848 + GFF4145 0.31 -0.4 153,848 + GFF4145 0.31 +0.9 153,947 + GFF4145 0.43 +1.4 153,947 + GFF4145 0.43 -0.1 154,080 - GFF4145 0.58 -0.4 154,448 + +1.8 154,449 - -0.7 154,449 - +0.1 154,467 + -0.1 154,468 - -0.6 154,498 - +0.8
Or see this region's nucleotide sequence