Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3855 and GFF3856 are separated by 206 nucleotides GFF3856 and GFF3857 overlap by 1 nucleotides GFF3857 and GFF3858 are separated by 54 nucleotides
GFF3855 - 5,6,7,8-tetrahydromethanopterin hydro-lyase, at 222,902 to 223,408
GFF3855
GFF3856 - Hydrazine synthase subunit beta, at 223,615 to 224,595
GFF3856
GFF3857 - hypothetical protein, at 224,595 to 225,131
GFF3857
GFF3858 - hypothetical protein, at 225,186 to 225,737
GFF3858
Position (kb)
223
224
225 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 222.826 kb on - strand at 222.834 kb on + strand at 222.835 kb on - strand at 222.875 kb on + strand at 222.907 kb on + strand at 222.908 kb on - strand at 222.913 kb on + strand at 222.914 kb on - strand at 223.166 kb on + strand, within GFF3855 at 223.298 kb on + strand, within GFF3855 at 223.299 kb on - strand, within GFF3855 at 223.305 kb on - strand, within GFF3855 at 223.407 kb on - strand at 223.494 kb on - strand at 223.582 kb on + strand at 223.739 kb on + strand, within GFF3856 at 223.739 kb on + strand, within GFF3856 at 223.740 kb on - strand, within GFF3856 at 223.793 kb on + strand, within GFF3856 at 223.850 kb on + strand, within GFF3856 at 223.896 kb on - strand, within GFF3856 at 223.983 kb on + strand, within GFF3856 at 223.983 kb on + strand, within GFF3856 at 223.986 kb on - strand, within GFF3856 at 223.986 kb on - strand, within GFF3856 at 224.081 kb on + strand, within GFF3856 at 224.222 kb on + strand, within GFF3856 at 224.222 kb on + strand, within GFF3856 at 224.223 kb on - strand, within GFF3856 at 224.237 kb on + strand, within GFF3856 at 224.238 kb on - strand, within GFF3856 at 224.238 kb on - strand, within GFF3856 at 224.436 kb on + strand, within GFF3856 at 224.644 kb on + strand at 224.645 kb on - strand at 224.720 kb on - strand, within GFF3857 at 224.788 kb on + strand, within GFF3857 at 224.788 kb on + strand, within GFF3857 at 224.788 kb on + strand, within GFF3857 at 224.788 kb on + strand, within GFF3857 at 224.789 kb on - strand, within GFF3857 at 224.789 kb on - strand, within GFF3857 at 224.789 kb on - strand, within GFF3857 at 224.789 kb on - strand, within GFF3857 at 224.789 kb on - strand, within GFF3857 at 224.839 kb on + strand, within GFF3857 at 224.839 kb on + strand, within GFF3857 at 224.840 kb on - strand, within GFF3857 at 224.840 kb on - strand, within GFF3857 at 224.840 kb on - strand, within GFF3857 at 224.840 kb on - strand, within GFF3857 at 225.239 kb on - strand at 225.281 kb on + strand, within GFF3858 at 225.458 kb on - strand, within GFF3858
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 222,826 - -4.0 222,834 + -2.5 222,835 - -4.6 222,875 + -3.7 222,907 + -2.4 222,908 - -2.3 222,913 + -1.8 222,914 - -2.1 223,166 + GFF3855 0.52 -1.5 223,298 + GFF3855 0.78 -2.2 223,299 - GFF3855 0.78 -2.8 223,305 - GFF3855 0.79 -2.9 223,407 - -1.5 223,494 - -3.6 223,582 + +0.4 223,739 + GFF3856 0.13 +0.0 223,739 + GFF3856 0.13 +0.7 223,740 - GFF3856 0.13 +0.2 223,793 + GFF3856 0.18 +0.4 223,850 + GFF3856 0.24 +0.1 223,896 - GFF3856 0.29 -1.1 223,983 + GFF3856 0.38 -1.0 223,983 + GFF3856 0.38 +1.2 223,986 - GFF3856 0.38 +0.2 223,986 - GFF3856 0.38 +0.5 224,081 + GFF3856 0.48 +0.5 224,222 + GFF3856 0.62 +0.5 224,222 + GFF3856 0.62 -0.8 224,223 - GFF3856 0.62 -2.8 224,237 + GFF3856 0.63 +1.3 224,238 - GFF3856 0.64 -1.3 224,238 - GFF3856 0.64 +1.4 224,436 + GFF3856 0.84 -0.8 224,644 + +1.9 224,645 - -0.6 224,720 - GFF3857 0.23 -0.9 224,788 + GFF3857 0.36 +1.6 224,788 + GFF3857 0.36 +0.4 224,788 + GFF3857 0.36 -0.1 224,788 + GFF3857 0.36 +0.7 224,789 - GFF3857 0.36 +0.2 224,789 - GFF3857 0.36 +1.1 224,789 - GFF3857 0.36 +0.8 224,789 - GFF3857 0.36 +2.4 224,789 - GFF3857 0.36 +0.5 224,839 + GFF3857 0.45 -0.8 224,839 + GFF3857 0.45 +0.7 224,840 - GFF3857 0.46 -0.7 224,840 - GFF3857 0.46 -0.2 224,840 - GFF3857 0.46 +1.6 224,840 - GFF3857 0.46 -0.3 225,239 - +1.0 225,281 + GFF3858 0.17 -1.1 225,458 - GFF3858 0.49 +0.9
Or see this region's nucleotide sequence