Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3076 and GFF3077 are separated by 97 nucleotides GFF3077 and GFF3078 overlap by 4 nucleotides GFF3078 and GFF3079 are separated by 435 nucleotides
GFF3076 - hypothetical protein, at 808,119 to 808,658
GFF3076
GFF3077 - Anti-sigma-E factor ChrR, at 808,756 to 809,451
GFF3077
GFF3078 - ECF RNA polymerase sigma factor RpoE, at 809,448 to 810,029
GFF3078
GFF3079 - 5,6,7,8-tetrahydromethanopterin hydro-lyase, at 810,465 to 811,004
GFF3079
Position (kb)
809
810
811 Strain fitness (log2 ratio)
-1
0
1 at 808.885 kb on + strand, within GFF3077 at 808.885 kb on + strand, within GFF3077 at 808.885 kb on + strand, within GFF3077 at 808.886 kb on - strand, within GFF3077 at 808.904 kb on + strand, within GFF3077 at 809.408 kb on + strand at 809.707 kb on + strand, within GFF3078 at 809.708 kb on - strand, within GFF3078 at 809.711 kb on + strand, within GFF3078 at 809.711 kb on + strand, within GFF3078 at 809.712 kb on - strand, within GFF3078 at 809.807 kb on - strand, within GFF3078 at 809.891 kb on - strand, within GFF3078 at 809.891 kb on - strand, within GFF3078 at 810.494 kb on - strand at 810.583 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 808,885 + GFF3077 0.19 +0.5 808,885 + GFF3077 0.19 -1.0 808,885 + GFF3077 0.19 +0.1 808,886 - GFF3077 0.19 +0.6 808,904 + GFF3077 0.21 +0.7 809,408 + +1.0 809,707 + GFF3078 0.45 -0.3 809,708 - GFF3078 0.45 -0.7 809,711 + GFF3078 0.45 +0.9 809,711 + GFF3078 0.45 +0.1 809,712 - GFF3078 0.45 +0.5 809,807 - GFF3078 0.62 +0.2 809,891 - GFF3078 0.76 +0.7 809,891 - GFF3078 0.76 -1.6 810,494 - -0.3 810,583 + GFF3079 0.22 +0.9 810,766 + GFF3079 0.56 -0.4 810,766 + GFF3079 0.56 -1.1 810,766 + GFF3079 0.56 +0.6 810,766 + GFF3079 0.56 -0.4
Or see this region's nucleotide sequence