Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1578 and GFF1579 are separated by 20 nucleotides GFF1579 and GFF1580 are separated by 13 nucleotides GFF1580 and GFF1581 overlap by 20 nucleotides GFF1581 and GFF1582 overlap by 4 nucleotides
GFF1578 - Apulose-4-phosphate transketolase subunit A, at 225,241 to 226,077
GFF1578
GFF1579 - 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase, at 226,098 to 226,841
GFF1579
GFF1580 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 226,855 to 227,571
GFF1580
GFF1581 - Lipopolysaccharide export system ATP-binding protein LptB, at 227,552 to 228,289
GFF1581
GFF1582 - hypothetical protein, at 228,286 to 229,332
GFF1582
Position (kb) 226
227
228 Strain fitness (log2 ratio) -2
-1
0
1 at 225.869 kb on + strand, within GFF1578 at 225.869 kb on + strand, within GFF1578 at 225.869 kb on + strand, within GFF1578 at 225.975 kb on - strand, within GFF1578 at 226.008 kb on + strand at 226.008 kb on + strand at 226.008 kb on + strand at 226.008 kb on + strand at 226.008 kb on + strand at 226.009 kb on - strand at 226.111 kb on + strand at 226.112 kb on - strand at 226.147 kb on + strand at 226.147 kb on + strand at 226.148 kb on - strand at 226.148 kb on - strand at 226.148 kb on - strand at 226.387 kb on + strand, within GFF1579 at 226.387 kb on + strand, within GFF1579 at 226.388 kb on - strand, within GFF1579 at 226.388 kb on - strand, within GFF1579 at 226.544 kb on - strand, within GFF1579 at 226.832 kb on + strand at 226.832 kb on + strand at 226.832 kb on + strand at 226.833 kb on - strand at 226.833 kb on - strand at 226.833 kb on - strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.836 kb on - strand at 226.868 kb on + strand at 226.868 kb on + strand at 226.868 kb on + strand at 226.868 kb on + strand at 226.868 kb on + strand at 226.868 kb on + strand at 226.869 kb on - strand at 226.869 kb on - strand at 226.869 kb on - strand at 226.947 kb on - strand, within GFF1580 at 227.095 kb on + strand, within GFF1580 at 227.095 kb on + strand, within GFF1580 at 227.241 kb on - strand, within GFF1580 at 227.241 kb on - strand, within GFF1580 at 227.528 kb on + strand at 227.529 kb on - strand at 227.529 kb on - strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.572 kb on - strand at 227.572 kb on - strand at 227.586 kb on + strand at 228.370 kb on - strand at 228.428 kb on + strand, within GFF1582 at 228.428 kb on + strand, within GFF1582 at 228.429 kb on - strand, within GFF1582 at 228.569 kb on + strand, within GFF1582 at 228.569 kb on + strand, within GFF1582 at 228.569 kb on + strand, within GFF1582
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 225,869 + GFF1578 0.75 +0.1 225,869 + GFF1578 0.75 -0.0 225,869 + GFF1578 0.75 -0.7 225,975 - GFF1578 0.88 +0.6 226,008 + -2.5 226,008 + -0.6 226,008 + -0.2 226,008 + -0.6 226,008 + +0.0 226,009 - -1.3 226,111 + -1.6 226,112 - -0.4 226,147 + -0.6 226,147 + +0.4 226,148 - +0.7 226,148 - +0.1 226,148 - -0.6 226,387 + GFF1579 0.39 +0.4 226,387 + GFF1579 0.39 +0.0 226,388 - GFF1579 0.39 +0.5 226,388 - GFF1579 0.39 +0.6 226,544 - GFF1579 0.60 -0.1 226,832 + -1.0 226,832 + -1.1 226,832 + +1.4 226,833 - +0.2 226,833 - -0.7 226,833 - -0.2 226,835 + -0.2 226,835 + -0.4 226,835 + -0.1 226,836 - +0.5 226,868 + -0.7 226,868 + -0.5 226,868 + +0.1 226,868 + -0.5 226,868 + +0.2 226,868 + +0.3 226,869 - +1.0 226,869 - -1.0 226,869 - +0.5 226,947 - GFF1580 0.13 +0.1 227,095 + GFF1580 0.33 -0.4 227,095 + GFF1580 0.33 +0.5 227,241 - GFF1580 0.54 +0.1 227,241 - GFF1580 0.54 -0.9 227,528 + +0.0 227,529 - -0.0 227,529 - -0.3 227,571 + -0.3 227,571 + -0.8 227,571 + +0.2 227,571 + +0.1 227,571 + -0.3 227,571 + -0.3 227,571 + +0.9 227,571 + -0.7 227,571 + -0.4 227,572 - +0.1 227,572 - -0.4 227,586 + +0.3 228,370 - -0.1 228,428 + GFF1582 0.14 -0.3 228,428 + GFF1582 0.14 -0.0 228,429 - GFF1582 0.14 -0.1 228,569 + GFF1582 0.27 +0.3 228,569 + GFF1582 0.27 -0.6 228,569 + GFF1582 0.27 +0.9
Or see this region's nucleotide sequence