Experiment: Mixed culture with Prochlorococcus marinus str. MED4; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01329 and MIT1002_01330 are separated by 3 nucleotides MIT1002_01330 and MIT1002_01331 overlap by 1 nucleotides MIT1002_01331 and MIT1002_01332 are separated by 36 nucleotides MIT1002_01332 and MIT1002_01333 are separated by 68 nucleotides
MIT1002_01329: MIT1002_01329 - Aspartate--tRNA ligase, at 1,475,726 to 1,477,486
_01329
MIT1002_01330: MIT1002_01330 - Dihydroneopterin triphosphate pyrophosphatase, at 1,477,490 to 1,477,930
_01330
MIT1002_01331: MIT1002_01331 - Crossover junction endodeoxyribonuclease RuvC, at 1,477,930 to 1,478,448
_01331
MIT1002_01332: MIT1002_01332 - Holliday junction ATP-dependent DNA helicase RuvA, at 1,478,485 to 1,479,105
_01332
MIT1002_01333: MIT1002_01333 - Holliday junction ATP-dependent DNA helicase RuvB, at 1,479,174 to 1,480,178
_01333
Position (kb)
1477
1478
1479 Strain fitness (log2 ratio)
-2
-1
0
1 at 1477.547 kb on + strand, within MIT1002_01330 at 1477.683 kb on + strand, within MIT1002_01330 at 1477.947 kb on + strand at 1478.443 kb on + strand at 1478.451 kb on - strand at 1478.453 kb on + strand at 1478.609 kb on + strand, within MIT1002_01332 at 1478.822 kb on - strand, within MIT1002_01332 at 1478.909 kb on - strand, within MIT1002_01332 at 1479.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture with Prochlorococcus marinus str. MED4; Experiment B, time point 2 remove 1,477,547 + MIT1002_01330 0.13 +1.0 1,477,683 + MIT1002_01330 0.44 -2.3 1,477,947 + -1.1 1,478,443 + +0.5 1,478,451 - -0.8 1,478,453 + +0.5 1,478,609 + MIT1002_01332 0.20 -1.1 1,478,822 - MIT1002_01332 0.54 -1.4 1,478,909 - MIT1002_01332 0.68 -1.4 1,479,164 - -0.8
Or see this region's nucleotide sequence