Experiment: monoculture; Experiment C, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02214 and MIT1002_02215 are separated by 76 nucleotides MIT1002_02215 and MIT1002_02216 are separated by 43 nucleotides MIT1002_02216 and MIT1002_02217 are separated by 77 nucleotides MIT1002_02217 and MIT1002_02218 are separated by 6 nucleotides
MIT1002_02214: MIT1002_02214 - NAD(P)H dehydrogenase (quinone), at 2,474,012 to 2,474,602
_02214
MIT1002_02215: MIT1002_02215 - putative membrane protein, at 2,474,679 to 2,475,101
_02215
MIT1002_02216: MIT1002_02216 - hypothetical protein, at 2,475,145 to 2,477,112
_02216
MIT1002_02217: MIT1002_02217 - hypothetical protein, at 2,477,190 to 2,477,927
_02217
MIT1002_02218: MIT1002_02218 - hypothetical protein, at 2,477,934 to 2,479,385
_02218
Position (kb)
2475
2476
2477
2478 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11
12 at 2474.170 kb on + strand, within MIT1002_02214 at 2474.190 kb on + strand, within MIT1002_02214 at 2474.192 kb on + strand, within MIT1002_02214 at 2474.198 kb on - strand, within MIT1002_02214 at 2474.200 kb on + strand, within MIT1002_02214 at 2474.200 kb on + strand, within MIT1002_02214 at 2474.208 kb on - strand, within MIT1002_02214 at 2474.422 kb on + strand, within MIT1002_02214 at 2474.430 kb on - strand, within MIT1002_02214 at 2474.430 kb on - strand, within MIT1002_02214 at 2474.438 kb on + strand, within MIT1002_02214 at 2474.438 kb on + strand, within MIT1002_02214 at 2474.438 kb on + strand, within MIT1002_02214 at 2474.443 kb on + strand, within MIT1002_02214 at 2474.446 kb on - strand, within MIT1002_02214 at 2474.448 kb on + strand, within MIT1002_02214 at 2474.449 kb on - strand, within MIT1002_02214 at 2474.475 kb on + strand, within MIT1002_02214 at 2474.483 kb on - strand, within MIT1002_02214 at 2474.483 kb on - strand, within MIT1002_02214 at 2474.483 kb on - strand, within MIT1002_02214 at 2474.505 kb on + strand, within MIT1002_02214 at 2474.508 kb on + strand, within MIT1002_02214 at 2474.516 kb on - strand, within MIT1002_02214 at 2474.542 kb on + strand, within MIT1002_02214 at 2474.544 kb on + strand at 2474.554 kb on + strand at 2474.554 kb on + strand at 2474.554 kb on + strand at 2474.554 kb on + strand at 2474.562 kb on - strand at 2474.562 kb on - strand at 2474.603 kb on - strand at 2474.605 kb on - strand at 2474.715 kb on + strand at 2474.715 kb on - strand at 2474.723 kb on - strand, within MIT1002_02215 at 2474.730 kb on + strand, within MIT1002_02215 at 2474.743 kb on + strand, within MIT1002_02215 at 2474.743 kb on + strand, within MIT1002_02215 at 2474.751 kb on - strand, within MIT1002_02215 at 2474.834 kb on + strand, within MIT1002_02215 at 2474.858 kb on + strand, within MIT1002_02215 at 2474.866 kb on - strand, within MIT1002_02215 at 2474.921 kb on + strand, within MIT1002_02215 at 2474.922 kb on - strand, within MIT1002_02215 at 2474.929 kb on - strand, within MIT1002_02215 at 2474.961 kb on - strand, within MIT1002_02215 at 2474.977 kb on + strand, within MIT1002_02215 at 2474.996 kb on - strand, within MIT1002_02215 at 2475.112 kb on - strand at 2475.235 kb on + strand at 2475.238 kb on - strand at 2475.351 kb on - strand, within MIT1002_02216 at 2475.351 kb on - strand, within MIT1002_02216 at 2475.364 kb on + strand, within MIT1002_02216 at 2475.413 kb on + strand, within MIT1002_02216 at 2475.413 kb on + strand, within MIT1002_02216 at 2475.413 kb on + strand, within MIT1002_02216 at 2475.427 kb on - strand, within MIT1002_02216 at 2475.449 kb on + strand, within MIT1002_02216 at 2475.545 kb on + strand, within MIT1002_02216 at 2475.638 kb on + strand, within MIT1002_02216 at 2475.638 kb on + strand, within MIT1002_02216 at 2475.644 kb on + strand, within MIT1002_02216 at 2475.646 kb on - strand, within MIT1002_02216 at 2475.646 kb on - strand, within MIT1002_02216 at 2475.646 kb on - strand, within MIT1002_02216 at 2475.658 kb on + strand, within MIT1002_02216 at 2475.765 kb on - strand, within MIT1002_02216 at 2475.772 kb on + strand, within MIT1002_02216 at 2475.882 kb on + strand, within MIT1002_02216 at 2475.882 kb on + strand, within MIT1002_02216 at 2475.882 kb on + strand, within MIT1002_02216 at 2475.882 kb on + strand, within MIT1002_02216 at 2475.890 kb on - strand, within MIT1002_02216 at 2475.890 kb on - strand, within MIT1002_02216 at 2475.890 kb on - strand, within MIT1002_02216 at 2475.890 kb on - strand, within MIT1002_02216 at 2476.004 kb on - strand, within MIT1002_02216 at 2476.015 kb on + strand, within MIT1002_02216 at 2476.015 kb on + strand, within MIT1002_02216 at 2476.127 kb on + strand, within MIT1002_02216 at 2476.214 kb on + strand, within MIT1002_02216 at 2476.214 kb on + strand, within MIT1002_02216 at 2476.216 kb on + strand, within MIT1002_02216 at 2476.216 kb on + strand, within MIT1002_02216 at 2476.222 kb on - strand, within MIT1002_02216 at 2476.222 kb on - strand, within MIT1002_02216 at 2476.224 kb on - strand, within MIT1002_02216 at 2476.230 kb on - strand, within MIT1002_02216 at 2476.584 kb on - strand, within MIT1002_02216 at 2476.603 kb on - strand, within MIT1002_02216 at 2476.619 kb on - strand, within MIT1002_02216 at 2476.678 kb on - strand, within MIT1002_02216 at 2476.917 kb on - strand at 2476.924 kb on - strand at 2477.114 kb on + strand at 2477.134 kb on + strand at 2477.137 kb on - strand at 2477.144 kb on + strand at 2477.144 kb on + strand at 2477.152 kb on - strand at 2477.152 kb on - strand at 2477.198 kb on + strand at 2477.206 kb on - strand at 2477.268 kb on + strand, within MIT1002_02217 at 2477.330 kb on - strand, within MIT1002_02217 at 2477.342 kb on + strand, within MIT1002_02217 at 2477.362 kb on + strand, within MIT1002_02217 at 2477.378 kb on - strand, within MIT1002_02217 at 2477.475 kb on - strand, within MIT1002_02217 at 2477.523 kb on - strand, within MIT1002_02217 at 2477.715 kb on - strand, within MIT1002_02217 at 2477.811 kb on + strand, within MIT1002_02217 at 2477.819 kb on - strand, within MIT1002_02217 at 2478.019 kb on - strand at 2478.094 kb on - strand, within MIT1002_02218
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 6 remove 2,474,170 + MIT1002_02214 0.27 +0.5 2,474,190 + MIT1002_02214 0.30 -1.1 2,474,192 + MIT1002_02214 0.30 -1.0 2,474,198 - MIT1002_02214 0.31 -0.6 2,474,200 + MIT1002_02214 0.32 -0.1 2,474,200 + MIT1002_02214 0.32 +1.8 2,474,208 - MIT1002_02214 0.33 -0.1 2,474,422 + MIT1002_02214 0.69 +0.3 2,474,430 - MIT1002_02214 0.71 -0.7 2,474,430 - MIT1002_02214 0.71 -1.8 2,474,438 + MIT1002_02214 0.72 +0.9 2,474,438 + MIT1002_02214 0.72 -1.6 2,474,438 + MIT1002_02214 0.72 +0.1 2,474,443 + MIT1002_02214 0.73 +0.8 2,474,446 - MIT1002_02214 0.73 +0.2 2,474,448 + MIT1002_02214 0.74 -1.7 2,474,449 - MIT1002_02214 0.74 -1.6 2,474,475 + MIT1002_02214 0.78 +1.4 2,474,483 - MIT1002_02214 0.80 -0.6 2,474,483 - MIT1002_02214 0.80 +0.1 2,474,483 - MIT1002_02214 0.80 +1.0 2,474,505 + MIT1002_02214 0.83 -0.8 2,474,508 + MIT1002_02214 0.84 -0.7 2,474,516 - MIT1002_02214 0.85 -0.3 2,474,542 + MIT1002_02214 0.90 -0.8 2,474,544 + -0.5 2,474,554 + -0.3 2,474,554 + +0.7 2,474,554 + -0.8 2,474,554 + -0.2 2,474,562 - -2.0 2,474,562 - +1.2 2,474,603 - +0.2 2,474,605 - +2.6 2,474,715 + -1.5 2,474,715 - -0.1 2,474,723 - MIT1002_02215 0.10 -0.2 2,474,730 + MIT1002_02215 0.12 +0.4 2,474,743 + MIT1002_02215 0.15 +1.1 2,474,743 + MIT1002_02215 0.15 +0.9 2,474,751 - MIT1002_02215 0.17 +0.6 2,474,834 + MIT1002_02215 0.37 -1.1 2,474,858 + MIT1002_02215 0.42 -0.1 2,474,866 - MIT1002_02215 0.44 -0.5 2,474,921 + MIT1002_02215 0.57 -0.6 2,474,922 - MIT1002_02215 0.57 -0.3 2,474,929 - MIT1002_02215 0.59 -2.1 2,474,961 - MIT1002_02215 0.67 -1.6 2,474,977 + MIT1002_02215 0.70 +1.2 2,474,996 - MIT1002_02215 0.75 -0.1 2,475,112 - +0.3 2,475,235 + +0.6 2,475,238 - +0.5 2,475,351 - MIT1002_02216 0.10 -2.0 2,475,351 - MIT1002_02216 0.10 -2.4 2,475,364 + MIT1002_02216 0.11 +0.6 2,475,413 + MIT1002_02216 0.14 -2.3 2,475,413 + MIT1002_02216 0.14 -0.1 2,475,413 + MIT1002_02216 0.14 +0.7 2,475,427 - MIT1002_02216 0.14 +1.5 2,475,449 + MIT1002_02216 0.15 -0.7 2,475,545 + MIT1002_02216 0.20 +0.8 2,475,638 + MIT1002_02216 0.25 -0.1 2,475,638 + MIT1002_02216 0.25 -2.5 2,475,644 + MIT1002_02216 0.25 -0.7 2,475,646 - MIT1002_02216 0.25 +1.6 2,475,646 - MIT1002_02216 0.25 +1.0 2,475,646 - MIT1002_02216 0.25 +0.4 2,475,658 + MIT1002_02216 0.26 -0.7 2,475,765 - MIT1002_02216 0.32 +0.8 2,475,772 + MIT1002_02216 0.32 -0.1 2,475,882 + MIT1002_02216 0.37 +0.4 2,475,882 + MIT1002_02216 0.37 -0.9 2,475,882 + MIT1002_02216 0.37 -0.4 2,475,882 + MIT1002_02216 0.37 -2.5 2,475,890 - MIT1002_02216 0.38 -0.2 2,475,890 - MIT1002_02216 0.38 +0.9 2,475,890 - MIT1002_02216 0.38 +0.7 2,475,890 - MIT1002_02216 0.38 -0.3 2,476,004 - MIT1002_02216 0.44 +2.1 2,476,015 + MIT1002_02216 0.44 +0.6 2,476,015 + MIT1002_02216 0.44 +0.4 2,476,127 + MIT1002_02216 0.50 +0.6 2,476,214 + MIT1002_02216 0.54 +0.6 2,476,214 + MIT1002_02216 0.54 +0.0 2,476,216 + MIT1002_02216 0.54 +12.3 2,476,216 + MIT1002_02216 0.54 +0.3 2,476,222 - MIT1002_02216 0.55 -0.6 2,476,222 - MIT1002_02216 0.55 -1.5 2,476,224 - MIT1002_02216 0.55 -0.7 2,476,230 - MIT1002_02216 0.55 -1.1 2,476,584 - MIT1002_02216 0.73 +0.1 2,476,603 - MIT1002_02216 0.74 -2.1 2,476,619 - MIT1002_02216 0.75 +1.7 2,476,678 - MIT1002_02216 0.78 +0.3 2,476,917 - -2.5 2,476,924 - -0.4 2,477,114 + +1.3 2,477,134 + +0.7 2,477,137 - -1.0 2,477,144 + +1.2 2,477,144 + +1.7 2,477,152 - -0.1 2,477,152 - -1.2 2,477,198 + -0.4 2,477,206 - -0.6 2,477,268 + MIT1002_02217 0.11 -1.7 2,477,330 - MIT1002_02217 0.19 -1.7 2,477,342 + MIT1002_02217 0.21 -0.0 2,477,362 + MIT1002_02217 0.23 +0.0 2,477,378 - MIT1002_02217 0.25 -1.2 2,477,475 - MIT1002_02217 0.39 -0.4 2,477,523 - MIT1002_02217 0.45 -1.1 2,477,715 - MIT1002_02217 0.71 -1.8 2,477,811 + MIT1002_02217 0.84 -2.0 2,477,819 - MIT1002_02217 0.85 -2.1 2,478,019 - +0.5 2,478,094 - MIT1002_02218 0.11 +1.7
Or see this region's nucleotide sequence