Experiment: monoculture; Experiment C, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00558 and MIT1002_00559 are separated by 66 nucleotides MIT1002_00559 and MIT1002_00560 are separated by 161 nucleotides MIT1002_00560 and MIT1002_00561 are separated by 40 nucleotides MIT1002_00561 and MIT1002_00562 are separated by 66 nucleotides
MIT1002_00558: MIT1002_00558 - hypothetical protein, at 605,532 to 605,864
_00558
MIT1002_00559: MIT1002_00559 - Gram-negative bacterial tonB protein, at 605,931 to 606,365
_00559
MIT1002_00560: MIT1002_00560 - DNA-binding transcriptional activator PspC, at 606,527 to 606,784
_00560
MIT1002_00561: MIT1002_00561 - thiol-disulfide oxidoreductase, at 606,825 to 607,364
_00561
MIT1002_00562: MIT1002_00562 - hypothetical protein, at 607,431 to 608,219
_00562
Position (kb)
606
607 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 605.704 kb on + strand, within MIT1002_00558 at 605.718 kb on - strand, within MIT1002_00558 at 605.772 kb on - strand, within MIT1002_00558 at 605.794 kb on - strand, within MIT1002_00558 at 605.833 kb on - strand at 605.866 kb on + strand at 605.874 kb on - strand at 605.981 kb on + strand, within MIT1002_00559 at 606.028 kb on - strand, within MIT1002_00559 at 606.157 kb on - strand, within MIT1002_00559 at 606.276 kb on - strand, within MIT1002_00559 at 606.284 kb on + strand, within MIT1002_00559 at 606.292 kb on - strand, within MIT1002_00559 at 606.302 kb on - strand, within MIT1002_00559 at 606.439 kb on + strand at 606.439 kb on + strand at 606.451 kb on + strand at 606.592 kb on + strand, within MIT1002_00560 at 606.592 kb on + strand, within MIT1002_00560 at 606.702 kb on - strand, within MIT1002_00560 at 606.710 kb on - strand, within MIT1002_00560 at 606.781 kb on + strand at 606.907 kb on + strand, within MIT1002_00561 at 606.950 kb on + strand, within MIT1002_00561 at 607.066 kb on - strand, within MIT1002_00561 at 607.066 kb on - strand, within MIT1002_00561 at 607.122 kb on - strand, within MIT1002_00561 at 607.122 kb on - strand, within MIT1002_00561 at 607.198 kb on - strand, within MIT1002_00561 at 607.216 kb on + strand, within MIT1002_00561 at 607.314 kb on - strand at 607.424 kb on - strand at 607.425 kb on - strand at 607.448 kb on - strand at 607.448 kb on - strand at 607.448 kb on - strand at 607.467 kb on - strand at 607.480 kb on + strand at 607.588 kb on + strand, within MIT1002_00562 at 607.596 kb on - strand, within MIT1002_00562 at 607.622 kb on + strand, within MIT1002_00562 at 607.658 kb on - strand, within MIT1002_00562 at 607.673 kb on - strand, within MIT1002_00562 at 607.706 kb on + strand, within MIT1002_00562 at 607.724 kb on + strand, within MIT1002_00562 at 607.724 kb on + strand, within MIT1002_00562 at 607.724 kb on + strand, within MIT1002_00562 at 607.724 kb on + strand, within MIT1002_00562 at 607.729 kb on + strand, within MIT1002_00562 at 607.729 kb on + strand, within MIT1002_00562 at 607.729 kb on + strand, within MIT1002_00562 at 607.732 kb on - strand, within MIT1002_00562 at 607.732 kb on - strand, within MIT1002_00562 at 607.732 kb on - strand, within MIT1002_00562 at 607.732 kb on - strand, within MIT1002_00562 at 607.766 kb on - strand, within MIT1002_00562
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 6 remove 605,704 + MIT1002_00558 0.52 +0.5 605,718 - MIT1002_00558 0.56 +1.2 605,772 - MIT1002_00558 0.72 +1.4 605,794 - MIT1002_00558 0.79 -0.5 605,833 - -0.9 605,866 + -0.4 605,874 - -0.6 605,981 + MIT1002_00559 0.11 +1.4 606,028 - MIT1002_00559 0.22 +1.0 606,157 - MIT1002_00559 0.52 +0.3 606,276 - MIT1002_00559 0.79 -2.5 606,284 + MIT1002_00559 0.81 +0.8 606,292 - MIT1002_00559 0.83 -0.5 606,302 - MIT1002_00559 0.85 -0.8 606,439 + +0.5 606,439 + +0.2 606,451 + +1.4 606,592 + MIT1002_00560 0.25 -1.3 606,592 + MIT1002_00560 0.25 -0.8 606,702 - MIT1002_00560 0.68 -0.3 606,710 - MIT1002_00560 0.71 -1.6 606,781 + -1.8 606,907 + MIT1002_00561 0.15 +1.0 606,950 + MIT1002_00561 0.23 +0.6 607,066 - MIT1002_00561 0.45 -0.2 607,066 - MIT1002_00561 0.45 -0.9 607,122 - MIT1002_00561 0.55 +2.9 607,122 - MIT1002_00561 0.55 +0.9 607,198 - MIT1002_00561 0.69 -0.2 607,216 + MIT1002_00561 0.72 -1.7 607,314 - +0.9 607,424 - +0.1 607,425 - +1.4 607,448 - -0.5 607,448 - -1.0 607,448 - +0.4 607,467 - -0.4 607,480 + +0.1 607,588 + MIT1002_00562 0.20 +1.4 607,596 - MIT1002_00562 0.21 -1.2 607,622 + MIT1002_00562 0.24 +0.2 607,658 - MIT1002_00562 0.29 -0.6 607,673 - MIT1002_00562 0.31 -1.3 607,706 + MIT1002_00562 0.35 -0.1 607,724 + MIT1002_00562 0.37 -0.3 607,724 + MIT1002_00562 0.37 -0.3 607,724 + MIT1002_00562 0.37 -0.7 607,724 + MIT1002_00562 0.37 +0.5 607,729 + MIT1002_00562 0.38 -0.4 607,729 + MIT1002_00562 0.38 -1.3 607,729 + MIT1002_00562 0.38 +1.5 607,732 - MIT1002_00562 0.38 -0.6 607,732 - MIT1002_00562 0.38 +0.4 607,732 - MIT1002_00562 0.38 +0.2 607,732 - MIT1002_00562 0.38 +1.2 607,766 - MIT1002_00562 0.42 +1.8
Or see this region's nucleotide sequence