Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4319 and GFF4320 are separated by 147 nucleotides GFF4320 and GFF4321 are separated by 110 nucleotides GFF4321 and GFF4322 are separated by 85 nucleotides
GFF4319 - hypothetical protein, at 98,601 to 99,848
GFF4319
GFF4320 - 2-hydroxychromene-2-carboxylate isomerase, at 99,996 to 100,610
GFF4320
GFF4321 - hypothetical protein, at 100,721 to 101,470
GFF4321
GFF4322 - Argininosuccinate synthase, at 101,556 to 102,785
GFF4322
Position (kb) 99
100
101 Strain fitness (log2 ratio) -1
0
1 at 99.328 kb on + strand, within GFF4319 at 99.329 kb on - strand, within GFF4319 at 99.626 kb on - strand, within GFF4319 at 99.664 kb on + strand, within GFF4319 at 99.664 kb on + strand, within GFF4319 at 99.796 kb on + strand at 99.797 kb on - strand at 99.797 kb on - strand at 99.797 kb on - strand at 99.797 kb on - strand at 99.838 kb on - strand at 100.415 kb on + strand, within GFF4320 at 100.415 kb on + strand, within GFF4320 at 100.436 kb on - strand, within GFF4320 at 100.459 kb on + strand, within GFF4320 at 100.460 kb on - strand, within GFF4320 at 100.558 kb on + strand at 100.565 kb on - strand at 100.582 kb on + strand at 100.585 kb on + strand at 100.585 kb on + strand at 100.627 kb on + strand at 100.628 kb on - strand at 100.628 kb on - strand at 101.083 kb on + strand, within GFF4321 at 101.515 kb on + strand at 101.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 99,328 + GFF4319 0.58 -0.8 99,329 - GFF4319 0.58 +0.5 99,626 - GFF4319 0.82 -0.4 99,664 + GFF4319 0.85 +0.1 99,664 + GFF4319 0.85 -0.4 99,796 + -0.4 99,797 - +1.1 99,797 - -0.1 99,797 - +0.6 99,797 - +0.3 99,838 - +1.5 100,415 + GFF4320 0.68 -0.6 100,415 + GFF4320 0.68 -1.3 100,436 - GFF4320 0.72 +0.3 100,459 + GFF4320 0.75 -0.5 100,460 - GFF4320 0.75 -0.0 100,558 + -0.6 100,565 - -0.2 100,582 + -0.2 100,585 + +0.3 100,585 + -0.4 100,627 + -0.4 100,628 - +0.7 100,628 - +0.5 101,083 + GFF4321 0.48 +0.6 101,515 + +0.4 101,517 + -0.1
Or see this region's nucleotide sequence