Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4020 and GFF4021 are separated by 91 nucleotides GFF4021 and GFF4022 are separated by 32 nucleotides
GFF4020 - Aliphatic amidase expression-regulating protein, at 3,443 to 4,669
GFF4020
GFF4021 - 2-keto-4-carboxy-3-hexenedioate hydratase, at 4,761 to 5,744
GFF4021
GFF4022 - Betaine aldehyde dehydrogenase, at 5,777 to 7,318
GFF4022
Position (kb)
4
5
6 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3.779 kb on + strand, within GFF4020 at 3.911 kb on + strand, within GFF4020 at 3.933 kb on - strand, within GFF4020 at 3.933 kb on - strand, within GFF4020 at 4.064 kb on + strand, within GFF4020 at 4.073 kb on + strand, within GFF4020 at 4.073 kb on + strand, within GFF4020 at 4.073 kb on + strand, within GFF4020 at 4.074 kb on - strand, within GFF4020 at 4.074 kb on - strand, within GFF4020 at 4.074 kb on - strand, within GFF4020 at 4.160 kb on + strand, within GFF4020 at 4.268 kb on + strand, within GFF4020 at 4.349 kb on + strand, within GFF4020 at 4.349 kb on + strand, within GFF4020 at 4.349 kb on + strand, within GFF4020 at 4.349 kb on + strand, within GFF4020 at 4.364 kb on + strand, within GFF4020 at 4.500 kb on - strand, within GFF4020 at 4.523 kb on + strand, within GFF4020 at 4.524 kb on - strand, within GFF4020 at 4.564 kb on + strand at 4.574 kb on + strand at 4.581 kb on - strand at 4.607 kb on + strand at 4.607 kb on + strand at 4.607 kb on + strand at 4.608 kb on - strand at 4.640 kb on + strand at 4.802 kb on + strand at 4.803 kb on - strand at 5.001 kb on + strand, within GFF4021 at 5.002 kb on - strand, within GFF4021 at 5.283 kb on + strand, within GFF4021 at 5.283 kb on + strand, within GFF4021 at 5.316 kb on + strand, within GFF4021 at 5.317 kb on - strand, within GFF4021 at 5.319 kb on + strand, within GFF4021 at 5.449 kb on - strand, within GFF4021 at 5.449 kb on - strand, within GFF4021 at 5.539 kb on - strand, within GFF4021 at 5.577 kb on + strand, within GFF4021 at 5.594 kb on - strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.609 kb on - strand, within GFF4021 at 5.609 kb on - strand, within GFF4021 at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.753 kb on + strand at 6.039 kb on - strand, within GFF4022 at 6.159 kb on - strand, within GFF4022 at 6.159 kb on - strand, within GFF4022 at 6.159 kb on - strand, within GFF4022 at 6.215 kb on + strand, within GFF4022 at 6.224 kb on + strand, within GFF4022 at 6.224 kb on + strand, within GFF4022 at 6.224 kb on + strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.353 kb on + strand, within GFF4022 at 6.629 kb on + strand, within GFF4022 at 6.630 kb on - strand, within GFF4022 at 6.630 kb on - strand, within GFF4022 at 6.630 kb on - strand, within GFF4022 at 6.707 kb on + strand, within GFF4022 at 6.708 kb on - strand, within GFF4022 at 6.708 kb on - strand, within GFF4022 at 6.708 kb on - strand, within GFF4022 at 6.708 kb on - strand, within GFF4022
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 3,779 + GFF4020 0.27 +0.4 3,911 + GFF4020 0.38 -0.5 3,933 - GFF4020 0.40 +0.2 3,933 - GFF4020 0.40 -0.7 4,064 + GFF4020 0.51 -0.0 4,073 + GFF4020 0.51 +0.0 4,073 + GFF4020 0.51 +2.6 4,073 + GFF4020 0.51 +0.3 4,074 - GFF4020 0.51 +0.8 4,074 - GFF4020 0.51 -0.6 4,074 - GFF4020 0.51 +1.0 4,160 + GFF4020 0.58 -1.7 4,268 + GFF4020 0.67 -0.6 4,349 + GFF4020 0.74 +0.2 4,349 + GFF4020 0.74 -1.9 4,349 + GFF4020 0.74 -0.7 4,349 + GFF4020 0.74 +0.3 4,364 + GFF4020 0.75 +0.4 4,500 - GFF4020 0.86 -0.6 4,523 + GFF4020 0.88 +0.1 4,524 - GFF4020 0.88 -1.1 4,564 + +0.3 4,574 + +0.3 4,581 - -0.4 4,607 + +0.2 4,607 + -0.2 4,607 + +0.1 4,608 - -0.6 4,640 + +0.2 4,802 + -0.8 4,803 - +2.4 5,001 + GFF4021 0.24 +0.1 5,002 - GFF4021 0.24 -1.2 5,283 + GFF4021 0.53 -0.9 5,283 + GFF4021 0.53 -0.4 5,316 + GFF4021 0.56 +0.6 5,317 - GFF4021 0.57 +0.7 5,319 + GFF4021 0.57 -0.2 5,449 - GFF4021 0.70 +0.9 5,449 - GFF4021 0.70 +0.5 5,539 - GFF4021 0.79 +0.0 5,577 + GFF4021 0.83 +0.5 5,594 - GFF4021 0.85 -0.2 5,608 + GFF4021 0.86 -1.1 5,608 + GFF4021 0.86 +1.1 5,608 + GFF4021 0.86 -0.1 5,608 + GFF4021 0.86 +1.1 5,608 + GFF4021 0.86 +0.4 5,609 - GFF4021 0.86 +1.1 5,609 - GFF4021 0.86 +0.9 5,665 + +0.3 5,665 + +2.1 5,665 + -0.5 5,665 + -0.4 5,665 + -0.1 5,665 + +1.2 5,665 + +0.4 5,666 - +0.5 5,666 - +0.3 5,666 - -0.2 5,666 - +0.6 5,666 - -0.3 5,666 - +0.2 5,666 - -0.2 5,666 - -2.1 5,753 + -0.7 6,039 - GFF4022 0.17 +1.0 6,159 - GFF4022 0.25 +0.3 6,159 - GFF4022 0.25 +1.4 6,159 - GFF4022 0.25 -0.1 6,215 + GFF4022 0.28 -0.6 6,224 + GFF4022 0.29 +0.5 6,224 + GFF4022 0.29 +0.5 6,224 + GFF4022 0.29 +0.4 6,225 - GFF4022 0.29 -0.1 6,225 - GFF4022 0.29 +0.2 6,225 - GFF4022 0.29 -0.4 6,225 - GFF4022 0.29 +0.7 6,353 + GFF4022 0.37 +0.2 6,629 + GFF4022 0.55 +0.5 6,630 - GFF4022 0.55 +0.4 6,630 - GFF4022 0.55 -0.1 6,630 - GFF4022 0.55 -0.6 6,707 + GFF4022 0.60 +0.1 6,708 - GFF4022 0.60 -0.5 6,708 - GFF4022 0.60 +1.0 6,708 - GFF4022 0.60 -0.9 6,708 - GFF4022 0.60 -0.9
Or see this region's nucleotide sequence