Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3814 and GFF3815 are separated by 78 nucleotides GFF3815 and GFF3816 overlap by 4 nucleotides GFF3816 and GFF3817 are separated by 33 nucleotides GFF3817 and GFF3818 are separated by 31 nucleotides
GFF3814 - Arabinose 5-phosphate isomerase KdsD, at 171,703 to 172,755
GFF3814
GFF3815 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase, at 172,834 to 173,448
GFF3815
GFF3816 - hypothetical protein, at 173,445 to 174,152
GFF3816
GFF3817 - Hydroxyacylglutathione hydrolase GloC, at 174,186 to 174,824
GFF3817
GFF3818 - putative N-octanoylanthranilate hydrolase AqdA1, at 174,856 to 175,686
GFF3818
Position (kb)
173
174
175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 172.767 kb on - strand at 172.845 kb on - strand at 172.845 kb on - strand at 172.845 kb on - strand at 172.953 kb on + strand, within GFF3815 at 173.009 kb on + strand, within GFF3815 at 173.010 kb on - strand, within GFF3815 at 173.184 kb on - strand, within GFF3815 at 173.198 kb on + strand, within GFF3815 at 173.321 kb on + strand, within GFF3815 at 173.327 kb on + strand, within GFF3815 at 173.327 kb on + strand, within GFF3815 at 173.379 kb on - strand, within GFF3815 at 173.512 kb on + strand at 173.512 kb on + strand at 173.513 kb on - strand at 173.657 kb on - strand, within GFF3816 at 173.657 kb on - strand, within GFF3816 at 173.662 kb on + strand, within GFF3816 at 174.067 kb on + strand, within GFF3816 at 174.068 kb on - strand, within GFF3816 at 174.068 kb on - strand, within GFF3816 at 174.322 kb on + strand, within GFF3817 at 174.323 kb on - strand, within GFF3817 at 174.363 kb on - strand, within GFF3817 at 174.488 kb on - strand, within GFF3817 at 174.488 kb on - strand, within GFF3817 at 174.527 kb on - strand, within GFF3817 at 174.826 kb on + strand at 175.065 kb on + strand, within GFF3818 at 175.065 kb on + strand, within GFF3818 at 175.066 kb on - strand, within GFF3818
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 172,767 - +0.5 172,845 - +0.0 172,845 - +1.1 172,845 - +0.4 172,953 + GFF3815 0.19 +0.8 173,009 + GFF3815 0.28 -0.1 173,010 - GFF3815 0.29 +0.7 173,184 - GFF3815 0.57 -1.5 173,198 + GFF3815 0.59 +2.0 173,321 + GFF3815 0.79 +0.4 173,327 + GFF3815 0.80 +0.8 173,327 + GFF3815 0.80 +2.2 173,379 - GFF3815 0.89 +0.3 173,512 + -2.2 173,512 + -3.3 173,513 - -1.9 173,657 - GFF3816 0.30 -0.7 173,657 - GFF3816 0.30 -1.7 173,662 + GFF3816 0.31 -2.1 174,067 + GFF3816 0.88 -2.9 174,068 - GFF3816 0.88 -2.0 174,068 - GFF3816 0.88 -1.9 174,322 + GFF3817 0.21 +0.7 174,323 - GFF3817 0.21 -0.4 174,363 - GFF3817 0.28 -0.3 174,488 - GFF3817 0.47 -0.3 174,488 - GFF3817 0.47 +0.0 174,527 - GFF3817 0.53 +0.3 174,826 + -0.2 175,065 + GFF3818 0.25 -0.1 175,065 + GFF3818 0.25 +1.3 175,066 - GFF3818 0.25 -1.0
Or see this region's nucleotide sequence