Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF32 and GFF33 are separated by 136 nucleotides GFF33 and GFF34 are separated by 62 nucleotides
GFF32 - Acetolactate synthase large subunit IlvG, at 38,520 to 40,232
GFF32
GFF33 - Beta-ketothiolase BktB, at 40,369 to 41,553
GFF33
GFF34 - (2R)-3-sulfolactate dehydrogenase (NADP(+)), at 41,616 to 42,647
GFF34
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1 at 39.482 kb on + strand, within GFF32 at 40.280 kb on + strand at 40.301 kb on + strand at 40.301 kb on + strand at 40.325 kb on - strand at 40.407 kb on + strand at 40.567 kb on + strand, within GFF33 at 40.567 kb on + strand, within GFF33 at 40.567 kb on + strand, within GFF33 at 40.567 kb on + strand, within GFF33 at 40.567 kb on + strand, within GFF33 at 40.568 kb on - strand, within GFF33 at 40.568 kb on - strand, within GFF33 at 40.568 kb on - strand, within GFF33 at 40.568 kb on - strand, within GFF33 at 40.568 kb on - strand, within GFF33 at 40.568 kb on - strand, within GFF33 at 40.568 kb on - strand, within GFF33 at 40.744 kb on + strand, within GFF33 at 40.745 kb on - strand, within GFF33 at 40.955 kb on - strand, within GFF33 at 40.955 kb on - strand, within GFF33 at 41.489 kb on - strand at 41.565 kb on + strand at 41.590 kb on - strand at 42.278 kb on - strand, within GFF34 at 42.466 kb on + strand, within GFF34 at 42.467 kb on - strand, within GFF34
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 39,482 + GFF32 0.56 +0.3 40,280 + +0.1 40,301 + -0.0 40,301 + +1.1 40,325 - +0.2 40,407 + +0.6 40,567 + GFF33 0.17 -0.5 40,567 + GFF33 0.17 -0.3 40,567 + GFF33 0.17 +1.0 40,567 + GFF33 0.17 -0.6 40,567 + GFF33 0.17 -0.6 40,568 - GFF33 0.17 +0.6 40,568 - GFF33 0.17 -0.4 40,568 - GFF33 0.17 +0.6 40,568 - GFF33 0.17 +0.4 40,568 - GFF33 0.17 -0.3 40,568 - GFF33 0.17 +0.2 40,568 - GFF33 0.17 -0.9 40,744 + GFF33 0.32 +0.7 40,745 - GFF33 0.32 -0.1 40,955 - GFF33 0.49 -1.8 40,955 - GFF33 0.49 +1.3 41,489 - -0.3 41,565 + +0.4 41,590 - -0.5 42,278 - GFF34 0.64 -0.3 42,466 + GFF34 0.82 -0.5 42,467 - GFF34 0.82 -0.8
Or see this region's nucleotide sequence