Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF265 and GFF266 overlap by 4 nucleotides GFF266 and GFF267 overlap by 4 nucleotides GFF267 and GFF268 are separated by 159 nucleotides GFF268 and GFF269 are separated by 59 nucleotides
GFF265 - Cysteine--tRNA ligase, at 279,768 to 281,156
GFF265
GFF266 - putative N-acetyltransferase YafP, at 281,153 to 281,641
GFF266
GFF267 - (R)-citramalate synthase, at 281,638 to 283,251
GFF267
GFF268 - hypothetical protein, at 283,411 to 283,857
GFF268
GFF269 - Acetyl-CoA:oxalate CoA-transferase, at 283,917 to 285,092
GFF269
Position (kb)
281
282
283
284 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 281.177 kb on + strand at 281.178 kb on - strand at 281.178 kb on - strand at 281.405 kb on + strand, within GFF266 at 281.405 kb on + strand, within GFF266 at 281.405 kb on + strand, within GFF266 at 281.405 kb on + strand, within GFF266 at 281.405 kb on + strand, within GFF266 at 281.406 kb on - strand, within GFF266 at 281.406 kb on - strand, within GFF266 at 281.406 kb on - strand, within GFF266 at 281.406 kb on - strand, within GFF266 at 281.406 kb on - strand, within GFF266 at 281.406 kb on - strand, within GFF266 at 281.406 kb on - strand, within GFF266 at 281.674 kb on + strand at 281.674 kb on + strand at 281.674 kb on + strand at 281.686 kb on - strand at 281.713 kb on + strand at 281.782 kb on + strand at 281.782 kb on + strand at 281.782 kb on + strand at 281.782 kb on + strand at 281.782 kb on + strand at 281.782 kb on + strand at 281.782 kb on + strand at 281.782 kb on + strand at 281.783 kb on - strand at 281.783 kb on - strand at 281.783 kb on - strand at 281.783 kb on - strand at 281.783 kb on - strand at 281.978 kb on - strand, within GFF267 at 281.978 kb on - strand, within GFF267 at 282.149 kb on - strand, within GFF267 at 282.424 kb on + strand, within GFF267 at 282.425 kb on - strand, within GFF267 at 282.442 kb on - strand, within GFF267 at 282.535 kb on + strand, within GFF267 at 282.535 kb on + strand, within GFF267 at 282.536 kb on - strand, within GFF267 at 282.536 kb on - strand, within GFF267 at 282.607 kb on + strand, within GFF267 at 282.607 kb on + strand, within GFF267 at 282.782 kb on - strand, within GFF267 at 282.787 kb on + strand, within GFF267 at 282.787 kb on + strand, within GFF267 at 282.788 kb on - strand, within GFF267 at 282.788 kb on - strand, within GFF267 at 282.893 kb on - strand, within GFF267 at 283.033 kb on + strand, within GFF267 at 283.034 kb on - strand, within GFF267 at 283.069 kb on + strand, within GFF267 at 283.069 kb on + strand, within GFF267 at 283.070 kb on - strand, within GFF267 at 283.070 kb on - strand, within GFF267 at 283.383 kb on - strand at 283.414 kb on + strand at 283.639 kb on + strand, within GFF268 at 283.639 kb on + strand, within GFF268 at 283.639 kb on + strand, within GFF268 at 283.639 kb on + strand, within GFF268 at 283.640 kb on - strand, within GFF268 at 283.640 kb on - strand, within GFF268 at 283.640 kb on - strand, within GFF268 at 283.640 kb on - strand, within GFF268 at 283.640 kb on - strand, within GFF268 at 283.681 kb on + strand, within GFF268 at 283.699 kb on + strand, within GFF268 at 283.700 kb on - strand, within GFF268 at 283.738 kb on + strand, within GFF268 at 283.738 kb on + strand, within GFF268 at 283.739 kb on - strand, within GFF268
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 281,177 + -0.0 281,178 - -1.6 281,178 - -2.2 281,405 + GFF266 0.52 +0.3 281,405 + GFF266 0.52 +0.2 281,405 + GFF266 0.52 -0.2 281,405 + GFF266 0.52 -1.9 281,405 + GFF266 0.52 +0.6 281,406 - GFF266 0.52 -1.1 281,406 - GFF266 0.52 -2.9 281,406 - GFF266 0.52 -1.5 281,406 - GFF266 0.52 -1.6 281,406 - GFF266 0.52 -0.8 281,406 - GFF266 0.52 -2.6 281,406 - GFF266 0.52 -1.2 281,674 + -3.1 281,674 + -1.9 281,674 + -1.5 281,686 - -1.6 281,713 + -3.4 281,782 + -2.2 281,782 + -0.4 281,782 + -1.6 281,782 + -1.2 281,782 + -1.6 281,782 + -1.4 281,782 + -0.9 281,782 + -2.2 281,783 - -1.7 281,783 - -1.2 281,783 - -1.6 281,783 - -2.2 281,783 - -2.9 281,978 - GFF267 0.21 -1.5 281,978 - GFF267 0.21 -1.3 282,149 - GFF267 0.32 -1.5 282,424 + GFF267 0.49 -2.1 282,425 - GFF267 0.49 -2.4 282,442 - GFF267 0.50 -1.4 282,535 + GFF267 0.56 -3.7 282,535 + GFF267 0.56 -2.9 282,536 - GFF267 0.56 -2.5 282,536 - GFF267 0.56 -2.0 282,607 + GFF267 0.60 -2.4 282,607 + GFF267 0.60 -2.0 282,782 - GFF267 0.71 -1.7 282,787 + GFF267 0.71 -2.9 282,787 + GFF267 0.71 -2.4 282,788 - GFF267 0.71 -2.1 282,788 - GFF267 0.71 -1.8 282,893 - GFF267 0.78 -2.7 283,033 + GFF267 0.86 -0.8 283,034 - GFF267 0.86 -3.4 283,069 + GFF267 0.89 -4.2 283,069 + GFF267 0.89 -1.4 283,070 - GFF267 0.89 -1.3 283,070 - GFF267 0.89 -1.0 283,383 - -0.5 283,414 + +0.1 283,639 + GFF268 0.51 -0.4 283,639 + GFF268 0.51 -0.5 283,639 + GFF268 0.51 +0.2 283,639 + GFF268 0.51 -1.4 283,640 - GFF268 0.51 +0.2 283,640 - GFF268 0.51 +0.7 283,640 - GFF268 0.51 +0.6 283,640 - GFF268 0.51 +0.5 283,640 - GFF268 0.51 +0.6 283,681 + GFF268 0.60 +1.0 283,699 + GFF268 0.64 +1.0 283,700 - GFF268 0.65 +0.2 283,738 + GFF268 0.73 -0.2 283,738 + GFF268 0.73 +0.9 283,739 - GFF268 0.73 +0.8
Or see this region's nucleotide sequence