Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1653 and GFF1654 are separated by 2 nucleotides GFF1654 and GFF1655 are separated by 15 nucleotides GFF1655 and GFF1656 overlap by 8 nucleotides
GFF1653 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 305,820 to 306,605
GFF1653
GFF1654 - Bifunctional chorismate mutase/prephenate dehydratase, at 306,608 to 307,474
GFF1654
GFF1655 - hypothetical protein, at 307,490 to 309,295
GFF1655
GFF1656 - NADH pyrophosphatase, at 309,288 to 310,226
GFF1656
Position (kb) 307
308
309
310 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 306.848 kb on + strand, within GFF1654 at 306.849 kb on - strand, within GFF1654 at 306.849 kb on - strand, within GFF1654 at 306.995 kb on - strand, within GFF1654 at 306.995 kb on - strand, within GFF1654 at 306.995 kb on - strand, within GFF1654 at 306.995 kb on - strand, within GFF1654 at 306.995 kb on - strand, within GFF1654 at 307.076 kb on + strand, within GFF1654 at 307.076 kb on + strand, within GFF1654 at 307.076 kb on + strand, within GFF1654 at 307.076 kb on + strand, within GFF1654 at 307.189 kb on + strand, within GFF1654 at 307.189 kb on + strand, within GFF1654 at 307.190 kb on - strand, within GFF1654 at 307.190 kb on - strand, within GFF1654 at 307.233 kb on - strand, within GFF1654 at 307.289 kb on + strand, within GFF1654 at 307.289 kb on + strand, within GFF1654 at 307.325 kb on + strand, within GFF1654 at 307.325 kb on + strand, within GFF1654 at 307.325 kb on + strand, within GFF1654 at 307.325 kb on + strand, within GFF1654 at 307.326 kb on - strand, within GFF1654 at 307.326 kb on - strand, within GFF1654 at 307.326 kb on - strand, within GFF1654 at 307.326 kb on - strand, within GFF1654 at 307.422 kb on - strand at 307.570 kb on + strand at 307.570 kb on + strand at 307.570 kb on + strand at 307.570 kb on + strand at 307.572 kb on + strand at 307.572 kb on + strand at 307.572 kb on + strand at 307.573 kb on - strand at 307.573 kb on - strand at 307.573 kb on - strand at 307.573 kb on - strand at 307.573 kb on - strand at 307.573 kb on - strand at 307.573 kb on - strand at 307.663 kb on - strand at 307.663 kb on - strand at 307.810 kb on + strand, within GFF1655 at 307.878 kb on - strand, within GFF1655 at 308.106 kb on + strand, within GFF1655 at 308.107 kb on - strand, within GFF1655 at 308.118 kb on + strand, within GFF1655 at 308.118 kb on + strand, within GFF1655 at 308.118 kb on + strand, within GFF1655 at 308.119 kb on - strand, within GFF1655 at 308.119 kb on - strand, within GFF1655 at 308.119 kb on - strand, within GFF1655 at 308.119 kb on - strand, within GFF1655 at 308.180 kb on + strand, within GFF1655 at 308.181 kb on - strand, within GFF1655 at 308.181 kb on - strand, within GFF1655 at 308.216 kb on - strand, within GFF1655 at 308.950 kb on - strand, within GFF1655 at 309.162 kb on + strand at 309.524 kb on - strand, within GFF1656 at 309.573 kb on + strand, within GFF1656 at 309.809 kb on + strand, within GFF1656 at 309.809 kb on + strand, within GFF1656 at 309.810 kb on - strand, within GFF1656 at 309.810 kb on - strand, within GFF1656 at 309.810 kb on - strand, within GFF1656 at 310.047 kb on + strand, within GFF1656 at 310.177 kb on + strand at 310.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 306,848 + GFF1654 0.28 -1.7 306,849 - GFF1654 0.28 -2.4 306,849 - GFF1654 0.28 -1.8 306,995 - GFF1654 0.45 -4.1 306,995 - GFF1654 0.45 -2.2 306,995 - GFF1654 0.45 -2.1 306,995 - GFF1654 0.45 -1.5 306,995 - GFF1654 0.45 -3.2 307,076 + GFF1654 0.54 -1.5 307,076 + GFF1654 0.54 -2.5 307,076 + GFF1654 0.54 -2.0 307,076 + GFF1654 0.54 -1.6 307,189 + GFF1654 0.67 -2.7 307,189 + GFF1654 0.67 -2.9 307,190 - GFF1654 0.67 -2.7 307,190 - GFF1654 0.67 -2.6 307,233 - GFF1654 0.72 -0.7 307,289 + GFF1654 0.79 -0.3 307,289 + GFF1654 0.79 -1.7 307,325 + GFF1654 0.83 -2.3 307,325 + GFF1654 0.83 -3.9 307,325 + GFF1654 0.83 -0.2 307,325 + GFF1654 0.83 -1.4 307,326 - GFF1654 0.83 -1.6 307,326 - GFF1654 0.83 -0.9 307,326 - GFF1654 0.83 +0.1 307,326 - GFF1654 0.83 -0.2 307,422 - -2.9 307,570 + -1.0 307,570 + -0.1 307,570 + +0.2 307,570 + -0.6 307,572 + -0.2 307,572 + +0.5 307,572 + +1.3 307,573 - -3.1 307,573 - +1.3 307,573 - +1.2 307,573 - +1.0 307,573 - -0.0 307,573 - +0.3 307,573 - +0.8 307,663 - -0.2 307,663 - +1.2 307,810 + GFF1655 0.18 -0.3 307,878 - GFF1655 0.21 -0.7 308,106 + GFF1655 0.34 +0.1 308,107 - GFF1655 0.34 +0.3 308,118 + GFF1655 0.35 +0.3 308,118 + GFF1655 0.35 -0.4 308,118 + GFF1655 0.35 -0.2 308,119 - GFF1655 0.35 -1.1 308,119 - GFF1655 0.35 +0.3 308,119 - GFF1655 0.35 +0.8 308,119 - GFF1655 0.35 -0.6 308,180 + GFF1655 0.38 -1.2 308,181 - GFF1655 0.38 -0.4 308,181 - GFF1655 0.38 -0.1 308,216 - GFF1655 0.40 +0.8 308,950 - GFF1655 0.81 +0.5 309,162 + -0.1 309,524 - GFF1656 0.25 +1.1 309,573 + GFF1656 0.30 +0.8 309,809 + GFF1656 0.55 -0.5 309,809 + GFF1656 0.55 +0.2 309,810 - GFF1656 0.56 +0.4 309,810 - GFF1656 0.56 -0.2 309,810 - GFF1656 0.56 -0.2 310,047 + GFF1656 0.81 -0.2 310,177 + -0.1 310,178 - +0.3
Or see this region's nucleotide sequence