Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_04167 and MIT1002_04168 are separated by 237 nucleotides MIT1002_04168 and MIT1002_04169 are separated by 166 nucleotides MIT1002_04169 and MIT1002_04170 are separated by 311 nucleotides MIT1002_04170 and MIT1002_04171 are separated by 19 nucleotides
MIT1002_04167: MIT1002_04167 - Antitoxin HipB, at 31,455 to 31,865
_04167
MIT1002_04168: MIT1002_04168 - hypothetical protein, at 32,103 to 32,294
_04168
MIT1002_04169: MIT1002_04169 - hypothetical protein, at 32,461 to 32,601
_04169
MIT1002_04170: MIT1002_04170 - hypothetical protein, at 32,913 to 33,218
_04170
MIT1002_04171: MIT1002_04171 - hypothetical protein, at 33,238 to 33,363
_04171
Position (kb)
32
33 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 31.465 kb on + strand at 31.974 kb on - strand at 32.072 kb on + strand at 32.080 kb on - strand at 32.091 kb on - strand at 32.110 kb on - strand at 32.139 kb on - strand, within MIT1002_04168 at 32.191 kb on - strand, within MIT1002_04168 at 32.199 kb on + strand, within MIT1002_04168 at 32.209 kb on + strand, within MIT1002_04168 at 32.245 kb on + strand, within MIT1002_04168 at 32.245 kb on + strand, within MIT1002_04168 at 32.253 kb on - strand, within MIT1002_04168 at 32.253 kb on - strand, within MIT1002_04168 at 32.253 kb on - strand, within MIT1002_04168 at 32.263 kb on - strand, within MIT1002_04168 at 32.472 kb on - strand at 32.524 kb on + strand, within MIT1002_04169 at 32.532 kb on - strand, within MIT1002_04169 at 32.532 kb on - strand, within MIT1002_04169 at 32.532 kb on - strand, within MIT1002_04169 at 32.532 kb on - strand, within MIT1002_04169 at 32.585 kb on + strand, within MIT1002_04169 at 32.604 kb on + strand at 32.604 kb on + strand at 32.604 kb on + strand at 32.636 kb on - strand at 32.641 kb on + strand at 32.641 kb on + strand at 32.641 kb on + strand at 32.712 kb on + strand at 32.720 kb on - strand at 32.720 kb on - strand at 32.761 kb on + strand at 32.769 kb on - strand at 32.769 kb on - strand at 32.771 kb on + strand at 32.771 kb on + strand at 32.779 kb on - strand at 32.779 kb on - strand at 32.779 kb on - strand at 32.784 kb on + strand at 32.792 kb on - strand at 32.794 kb on + strand at 32.863 kb on + strand at 32.873 kb on + strand at 32.971 kb on - strand, within MIT1002_04170 at 32.987 kb on + strand, within MIT1002_04170 at 33.033 kb on - strand, within MIT1002_04170 at 33.036 kb on - strand, within MIT1002_04170 at 33.050 kb on + strand, within MIT1002_04170 at 33.051 kb on - strand, within MIT1002_04170 at 33.058 kb on - strand, within MIT1002_04170 at 33.099 kb on + strand, within MIT1002_04170 at 33.127 kb on + strand, within MIT1002_04170 at 33.127 kb on + strand, within MIT1002_04170 at 33.127 kb on + strand, within MIT1002_04170 at 33.127 kb on + strand, within MIT1002_04170 at 33.135 kb on - strand, within MIT1002_04170 at 33.135 kb on - strand, within MIT1002_04170 at 33.135 kb on - strand, within MIT1002_04170 at 33.135 kb on - strand, within MIT1002_04170 at 33.135 kb on - strand, within MIT1002_04170 at 33.179 kb on + strand, within MIT1002_04170 at 33.179 kb on + strand, within MIT1002_04170 at 33.179 kb on + strand, within MIT1002_04170 at 33.179 kb on + strand, within MIT1002_04170 at 33.179 kb on + strand, within MIT1002_04170 at 33.179 kb on + strand, within MIT1002_04170 at 33.179 kb on + strand, within MIT1002_04170 at 33.187 kb on - strand, within MIT1002_04170 at 33.187 kb on - strand, within MIT1002_04170 at 33.187 kb on - strand, within MIT1002_04170 at 33.187 kb on - strand, within MIT1002_04170 at 33.187 kb on - strand, within MIT1002_04170 at 33.187 kb on - strand, within MIT1002_04170 at 33.188 kb on - strand at 33.208 kb on + strand at 33.208 kb on + strand at 33.208 kb on - strand at 33.208 kb on - strand at 33.216 kb on - strand at 33.265 kb on - strand, within MIT1002_04171 at 33.265 kb on - strand, within MIT1002_04171 at 33.329 kb on - strand, within MIT1002_04171 at 33.386 kb on + strand at 33.431 kb on - strand at 33.461 kb on + strand at 33.469 kb on - strand at 33.478 kb on + strand at 33.478 kb on - strand at 33.546 kb on - strand at 33.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 31,465 + +0.3 31,974 - -1.3 32,072 + -0.5 32,080 - +1.0 32,091 - -2.1 32,110 - +0.6 32,139 - MIT1002_04168 0.19 +0.0 32,191 - MIT1002_04168 0.46 -0.5 32,199 + MIT1002_04168 0.50 -1.7 32,209 + MIT1002_04168 0.55 -0.3 32,245 + MIT1002_04168 0.74 +0.9 32,245 + MIT1002_04168 0.74 +0.6 32,253 - MIT1002_04168 0.78 -0.0 32,253 - MIT1002_04168 0.78 -0.5 32,253 - MIT1002_04168 0.78 +1.1 32,263 - MIT1002_04168 0.83 +0.5 32,472 - -0.1 32,524 + MIT1002_04169 0.45 +1.2 32,532 - MIT1002_04169 0.50 +0.6 32,532 - MIT1002_04169 0.50 +1.8 32,532 - MIT1002_04169 0.50 +0.2 32,532 - MIT1002_04169 0.50 -1.1 32,585 + MIT1002_04169 0.88 +0.1 32,604 + -0.4 32,604 + -0.0 32,604 + -1.2 32,636 - +0.4 32,641 + -1.6 32,641 + -0.3 32,641 + -0.2 32,712 + -0.9 32,720 - +1.4 32,720 - -0.2 32,761 + -0.9 32,769 - -0.7 32,769 - -2.2 32,771 + -0.7 32,771 + -0.9 32,779 - -1.6 32,779 - -0.7 32,779 - -0.4 32,784 + -0.9 32,792 - +1.7 32,794 + -1.0 32,863 + -0.7 32,873 + -0.8 32,971 - MIT1002_04170 0.19 +0.9 32,987 + MIT1002_04170 0.24 +1.4 33,033 - MIT1002_04170 0.39 +0.0 33,036 - MIT1002_04170 0.40 +1.3 33,050 + MIT1002_04170 0.45 -1.1 33,051 - MIT1002_04170 0.45 -3.2 33,058 - MIT1002_04170 0.47 -0.3 33,099 + MIT1002_04170 0.61 -2.0 33,127 + MIT1002_04170 0.70 -3.4 33,127 + MIT1002_04170 0.70 +0.3 33,127 + MIT1002_04170 0.70 -1.0 33,127 + MIT1002_04170 0.70 -1.2 33,135 - MIT1002_04170 0.73 -0.2 33,135 - MIT1002_04170 0.73 -0.7 33,135 - MIT1002_04170 0.73 +1.1 33,135 - MIT1002_04170 0.73 +0.3 33,135 - MIT1002_04170 0.73 +1.7 33,179 + MIT1002_04170 0.87 -1.8 33,179 + MIT1002_04170 0.87 -1.6 33,179 + MIT1002_04170 0.87 +1.4 33,179 + MIT1002_04170 0.87 -0.4 33,179 + MIT1002_04170 0.87 +0.0 33,179 + MIT1002_04170 0.87 +0.5 33,179 + MIT1002_04170 0.87 +0.2 33,187 - MIT1002_04170 0.90 -0.6 33,187 - MIT1002_04170 0.90 -1.4 33,187 - MIT1002_04170 0.90 +0.1 33,187 - MIT1002_04170 0.90 -0.2 33,187 - MIT1002_04170 0.90 -0.6 33,187 - MIT1002_04170 0.90 -2.0 33,188 - +0.8 33,208 + +0.9 33,208 + -1.0 33,208 - -0.4 33,208 - +1.2 33,216 - -0.6 33,265 - MIT1002_04171 0.21 +0.3 33,265 - MIT1002_04171 0.21 -0.4 33,329 - MIT1002_04171 0.72 +0.3 33,386 + +0.9 33,431 - -0.3 33,461 + -1.0 33,469 - -0.2 33,478 + -0.5 33,478 - -0.0 33,546 - -0.1 33,572 + +2.0
Or see this region's nucleotide sequence