Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03167 and MIT1002_03168 are separated by 153 nucleotides MIT1002_03168 and MIT1002_03169 are separated by 190 nucleotides MIT1002_03169 and MIT1002_03170 are separated by 76 nucleotides
MIT1002_03167: MIT1002_03167 - Fumarate hydratase class II, at 3,529,935 to 3,531,308
_03167
MIT1002_03168: MIT1002_03168 - hypothetical protein, at 3,531,462 to 3,531,800
_03168
MIT1002_03169: MIT1002_03169 - Azurin, at 3,531,991 to 3,532,440
_03169
MIT1002_03170: MIT1002_03170 - hypothetical protein, at 3,532,517 to 3,533,752
_03170
Position (kb)
3531
3532
3533 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8 at 3531.027 kb on - strand, within MIT1002_03167 at 3531.119 kb on - strand, within MIT1002_03167 at 3531.124 kb on + strand, within MIT1002_03167 at 3531.174 kb on + strand at 3531.174 kb on + strand at 3531.180 kb on + strand at 3531.180 kb on + strand at 3531.182 kb on - strand at 3531.188 kb on - strand at 3531.216 kb on - strand at 3531.230 kb on + strand at 3531.263 kb on + strand at 3531.263 kb on + strand at 3531.280 kb on - strand at 3531.282 kb on + strand at 3531.439 kb on + strand at 3531.455 kb on - strand at 3531.457 kb on - strand at 3531.550 kb on + strand, within MIT1002_03168 at 3531.550 kb on + strand, within MIT1002_03168 at 3531.551 kb on - strand, within MIT1002_03168 at 3531.558 kb on - strand, within MIT1002_03168 at 3531.558 kb on - strand, within MIT1002_03168 at 3531.580 kb on + strand, within MIT1002_03168 at 3531.671 kb on + strand, within MIT1002_03168 at 3531.779 kb on - strand at 3531.809 kb on - strand at 3532.018 kb on + strand at 3532.018 kb on + strand at 3532.073 kb on + strand, within MIT1002_03169 at 3532.149 kb on + strand, within MIT1002_03169 at 3532.183 kb on + strand, within MIT1002_03169 at 3532.183 kb on + strand, within MIT1002_03169 at 3532.191 kb on - strand, within MIT1002_03169 at 3532.191 kb on - strand, within MIT1002_03169 at 3532.326 kb on - strand, within MIT1002_03169 at 3532.345 kb on + strand, within MIT1002_03169 at 3532.352 kb on + strand, within MIT1002_03169 at 3532.401 kb on + strand at 3532.404 kb on - strand at 3532.443 kb on - strand at 3532.485 kb on - strand at 3532.496 kb on - strand at 3532.506 kb on - strand at 3532.514 kb on + strand at 3532.534 kb on - strand at 3532.570 kb on + strand at 3532.619 kb on + strand at 3532.651 kb on + strand, within MIT1002_03170 at 3532.651 kb on + strand, within MIT1002_03170 at 3532.651 kb on + strand, within MIT1002_03170 at 3532.688 kb on + strand, within MIT1002_03170 at 3532.695 kb on + strand, within MIT1002_03170 at 3532.735 kb on - strand, within MIT1002_03170 at 3532.775 kb on - strand, within MIT1002_03170 at 3532.821 kb on + strand, within MIT1002_03170 at 3532.823 kb on + strand, within MIT1002_03170 at 3532.829 kb on - strand, within MIT1002_03170 at 3532.829 kb on - strand, within MIT1002_03170 at 3532.829 kb on - strand, within MIT1002_03170 at 3532.842 kb on + strand, within MIT1002_03170 at 3532.852 kb on - strand, within MIT1002_03170 at 3532.852 kb on - strand, within MIT1002_03170 at 3532.859 kb on + strand, within MIT1002_03170 at 3532.859 kb on + strand, within MIT1002_03170 at 3532.867 kb on - strand, within MIT1002_03170 at 3532.867 kb on - strand, within MIT1002_03170 at 3532.867 kb on - strand, within MIT1002_03170 at 3532.867 kb on - strand, within MIT1002_03170 at 3532.885 kb on - strand, within MIT1002_03170 at 3532.937 kb on + strand, within MIT1002_03170 at 3532.942 kb on + strand, within MIT1002_03170 at 3532.942 kb on + strand, within MIT1002_03170 at 3532.950 kb on + strand, within MIT1002_03170 at 3532.988 kb on + strand, within MIT1002_03170 at 3533.029 kb on + strand, within MIT1002_03170 at 3533.051 kb on - strand, within MIT1002_03170 at 3533.180 kb on + strand, within MIT1002_03170 at 3533.182 kb on - strand, within MIT1002_03170 at 3533.233 kb on + strand, within MIT1002_03170 at 3533.233 kb on + strand, within MIT1002_03170 at 3533.233 kb on + strand, within MIT1002_03170 at 3533.233 kb on - strand, within MIT1002_03170 at 3533.241 kb on - strand, within MIT1002_03170 at 3533.287 kb on - strand, within MIT1002_03170 at 3533.302 kb on + strand, within MIT1002_03170 at 3533.303 kb on - strand, within MIT1002_03170 at 3533.339 kb on - strand, within MIT1002_03170 at 3533.416 kb on + strand, within MIT1002_03170 at 3533.416 kb on + strand, within MIT1002_03170
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 3,531,027 - MIT1002_03167 0.79 -0.6 3,531,119 - MIT1002_03167 0.86 -0.8 3,531,124 + MIT1002_03167 0.87 -0.2 3,531,174 + +0.4 3,531,174 + -0.5 3,531,180 + +0.3 3,531,180 + -1.0 3,531,182 - +0.6 3,531,188 - +0.7 3,531,216 - -0.1 3,531,230 + -0.1 3,531,263 + +1.1 3,531,263 + +0.5 3,531,280 - +1.9 3,531,282 + -0.8 3,531,439 + -1.1 3,531,455 - +0.6 3,531,457 - +3.4 3,531,550 + MIT1002_03168 0.26 +0.7 3,531,550 + MIT1002_03168 0.26 -0.9 3,531,551 - MIT1002_03168 0.26 +0.6 3,531,558 - MIT1002_03168 0.28 -0.3 3,531,558 - MIT1002_03168 0.28 -0.6 3,531,580 + MIT1002_03168 0.35 +1.1 3,531,671 + MIT1002_03168 0.62 -0.7 3,531,779 - -2.4 3,531,809 - +0.3 3,532,018 + +0.4 3,532,018 + +0.3 3,532,073 + MIT1002_03169 0.18 +0.2 3,532,149 + MIT1002_03169 0.35 +8.3 3,532,183 + MIT1002_03169 0.43 +1.0 3,532,183 + MIT1002_03169 0.43 -0.0 3,532,191 - MIT1002_03169 0.44 +0.4 3,532,191 - MIT1002_03169 0.44 -0.6 3,532,326 - MIT1002_03169 0.74 +0.7 3,532,345 + MIT1002_03169 0.79 +2.4 3,532,352 + MIT1002_03169 0.80 +0.7 3,532,401 + -0.8 3,532,404 - +0.1 3,532,443 - +1.9 3,532,485 - +0.6 3,532,496 - +0.6 3,532,506 - -0.4 3,532,514 + +0.5 3,532,534 - -0.8 3,532,570 + -0.2 3,532,619 + +1.4 3,532,651 + MIT1002_03170 0.11 -0.3 3,532,651 + MIT1002_03170 0.11 +2.2 3,532,651 + MIT1002_03170 0.11 +0.7 3,532,688 + MIT1002_03170 0.14 +0.8 3,532,695 + MIT1002_03170 0.14 +0.7 3,532,735 - MIT1002_03170 0.18 -0.5 3,532,775 - MIT1002_03170 0.21 -1.1 3,532,821 + MIT1002_03170 0.25 -0.3 3,532,823 + MIT1002_03170 0.25 +0.5 3,532,829 - MIT1002_03170 0.25 +1.0 3,532,829 - MIT1002_03170 0.25 +0.1 3,532,829 - MIT1002_03170 0.25 +0.6 3,532,842 + MIT1002_03170 0.26 -0.2 3,532,852 - MIT1002_03170 0.27 -0.8 3,532,852 - MIT1002_03170 0.27 +1.4 3,532,859 + MIT1002_03170 0.28 -0.6 3,532,859 + MIT1002_03170 0.28 +0.2 3,532,867 - MIT1002_03170 0.28 -0.1 3,532,867 - MIT1002_03170 0.28 -0.5 3,532,867 - MIT1002_03170 0.28 +0.4 3,532,867 - MIT1002_03170 0.28 +0.9 3,532,885 - MIT1002_03170 0.30 -0.8 3,532,937 + MIT1002_03170 0.34 +0.5 3,532,942 + MIT1002_03170 0.34 -0.6 3,532,942 + MIT1002_03170 0.34 -0.1 3,532,950 + MIT1002_03170 0.35 -0.3 3,532,988 + MIT1002_03170 0.38 +0.9 3,533,029 + MIT1002_03170 0.41 -0.8 3,533,051 - MIT1002_03170 0.43 +1.6 3,533,180 + MIT1002_03170 0.54 +0.7 3,533,182 - MIT1002_03170 0.54 +0.9 3,533,233 + MIT1002_03170 0.58 +0.5 3,533,233 + MIT1002_03170 0.58 -0.1 3,533,233 + MIT1002_03170 0.58 -1.7 3,533,233 - MIT1002_03170 0.58 +1.2 3,533,241 - MIT1002_03170 0.59 +0.4 3,533,287 - MIT1002_03170 0.62 +0.7 3,533,302 + MIT1002_03170 0.64 +0.2 3,533,303 - MIT1002_03170 0.64 -0.1 3,533,339 - MIT1002_03170 0.67 -1.9 3,533,416 + MIT1002_03170 0.73 -0.8 3,533,416 + MIT1002_03170 0.73 -0.5
Or see this region's nucleotide sequence