Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01685 and MIT1002_01686 are separated by 257 nucleotides MIT1002_01686 and MIT1002_01687 are separated by 284 nucleotides MIT1002_01687 and MIT1002_01688 are separated by 101 nucleotides
MIT1002_01685: MIT1002_01685 - ATP-dependent RNA helicase HrpB, at 1,871,317 to 1,875,312
_01685
MIT1002_01686: MIT1002_01686 - putative glycosyltransferase, at 1,875,570 to 1,875,872
_01686
MIT1002_01687: MIT1002_01687 - hypothetical protein, at 1,876,157 to 1,876,414
_01687
MIT1002_01688: MIT1002_01688 - Ribosomal RNA large subunit methyltransferase I, at 1,876,516 to 1,877,709
_01688
Position (kb)
1875
1876 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1874.614 kb on - strand, within MIT1002_01685 at 1874.828 kb on + strand, within MIT1002_01685 at 1874.850 kb on + strand, within MIT1002_01685 at 1874.863 kb on + strand, within MIT1002_01685 at 1874.871 kb on - strand, within MIT1002_01685 at 1874.871 kb on - strand, within MIT1002_01685 at 1874.893 kb on + strand, within MIT1002_01685 at 1874.894 kb on + strand, within MIT1002_01685 at 1874.894 kb on + strand, within MIT1002_01685 at 1874.894 kb on + strand, within MIT1002_01685 at 1874.895 kb on - strand, within MIT1002_01685 at 1874.902 kb on - strand, within MIT1002_01685 at 1874.933 kb on + strand at 1874.936 kb on + strand at 1874.960 kb on + strand at 1874.960 kb on + strand at 1874.960 kb on + strand at 1874.966 kb on - strand at 1874.970 kb on + strand at 1875.032 kb on + strand at 1875.032 kb on + strand at 1875.062 kb on - strand at 1875.074 kb on + strand at 1875.074 kb on + strand at 1875.128 kb on - strand at 1875.157 kb on - strand at 1875.186 kb on + strand at 1875.282 kb on + strand at 1875.282 kb on + strand at 1875.289 kb on + strand at 1875.289 kb on + strand at 1875.324 kb on + strand at 1875.326 kb on + strand at 1875.326 kb on + strand at 1875.326 kb on + strand at 1875.334 kb on - strand at 1875.344 kb on + strand at 1875.371 kb on - strand at 1875.478 kb on + strand at 1875.522 kb on - strand at 1875.530 kb on - strand at 1875.540 kb on + strand at 1875.658 kb on + strand, within MIT1002_01686 at 1875.674 kb on + strand, within MIT1002_01686 at 1875.696 kb on + strand, within MIT1002_01686 at 1875.731 kb on + strand, within MIT1002_01686 at 1875.732 kb on - strand, within MIT1002_01686 at 1875.732 kb on - strand, within MIT1002_01686 at 1875.800 kb on - strand, within MIT1002_01686 at 1875.804 kb on + strand, within MIT1002_01686 at 1875.804 kb on + strand, within MIT1002_01686 at 1875.804 kb on + strand, within MIT1002_01686 at 1875.812 kb on - strand, within MIT1002_01686 at 1875.838 kb on + strand, within MIT1002_01686 at 1875.838 kb on + strand, within MIT1002_01686 at 1875.879 kb on - strand at 1875.946 kb on - strand at 1875.990 kb on + strand at 1875.990 kb on + strand at 1875.998 kb on - strand at 1876.047 kb on + strand at 1876.096 kb on - strand at 1876.173 kb on + strand at 1876.173 kb on + strand at 1876.181 kb on - strand at 1876.245 kb on + strand, within MIT1002_01687 at 1876.270 kb on + strand, within MIT1002_01687 at 1876.342 kb on + strand, within MIT1002_01687 at 1876.394 kb on + strand at 1876.402 kb on - strand at 1876.409 kb on - strand at 1876.457 kb on - strand at 1876.537 kb on + strand at 1876.537 kb on + strand at 1876.538 kb on - strand at 1876.545 kb on - strand at 1876.686 kb on + strand, within MIT1002_01688 at 1876.694 kb on - strand, within MIT1002_01688 at 1876.696 kb on + strand, within MIT1002_01688 at 1876.699 kb on - strand, within MIT1002_01688 at 1876.704 kb on - strand, within MIT1002_01688 at 1876.713 kb on - strand, within MIT1002_01688 at 1876.731 kb on + strand, within MIT1002_01688 at 1876.776 kb on + strand, within MIT1002_01688 at 1876.777 kb on + strand, within MIT1002_01688 at 1876.777 kb on + strand, within MIT1002_01688 at 1876.785 kb on - strand, within MIT1002_01688 at 1876.843 kb on - strand, within MIT1002_01688 at 1876.863 kb on - strand, within MIT1002_01688 at 1876.865 kb on + strand, within MIT1002_01688
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 1,874,614 - MIT1002_01685 0.83 -0.3 1,874,828 + MIT1002_01685 0.88 +0.9 1,874,850 + MIT1002_01685 0.88 -0.9 1,874,863 + MIT1002_01685 0.89 +1.4 1,874,871 - MIT1002_01685 0.89 +0.3 1,874,871 - MIT1002_01685 0.89 -0.1 1,874,893 + MIT1002_01685 0.89 +3.2 1,874,894 + MIT1002_01685 0.90 +0.9 1,874,894 + MIT1002_01685 0.90 -2.5 1,874,894 + MIT1002_01685 0.90 -0.5 1,874,895 - MIT1002_01685 0.90 -0.5 1,874,902 - MIT1002_01685 0.90 -1.4 1,874,933 + -2.9 1,874,936 + -1.0 1,874,960 + -1.3 1,874,960 + +0.2 1,874,960 + -2.4 1,874,966 - -1.2 1,874,970 + -1.0 1,875,032 + +1.2 1,875,032 + +0.6 1,875,062 - -0.3 1,875,074 + -0.8 1,875,074 + -1.1 1,875,128 - -0.5 1,875,157 - -1.1 1,875,186 + +1.5 1,875,282 + +0.3 1,875,282 + +1.2 1,875,289 + +0.8 1,875,289 + -0.6 1,875,324 + -1.8 1,875,326 + +1.1 1,875,326 + -0.5 1,875,326 + +0.8 1,875,334 - -0.1 1,875,344 + -2.1 1,875,371 - -0.0 1,875,478 + +0.5 1,875,522 - -0.4 1,875,530 - +0.3 1,875,540 + -0.3 1,875,658 + MIT1002_01686 0.29 +2.2 1,875,674 + MIT1002_01686 0.34 -0.4 1,875,696 + MIT1002_01686 0.42 +0.0 1,875,731 + MIT1002_01686 0.53 -0.1 1,875,732 - MIT1002_01686 0.53 +0.6 1,875,732 - MIT1002_01686 0.53 +3.5 1,875,800 - MIT1002_01686 0.76 +0.6 1,875,804 + MIT1002_01686 0.77 +0.6 1,875,804 + MIT1002_01686 0.77 +2.9 1,875,804 + MIT1002_01686 0.77 -0.4 1,875,812 - MIT1002_01686 0.80 -0.2 1,875,838 + MIT1002_01686 0.88 +0.4 1,875,838 + MIT1002_01686 0.88 +0.8 1,875,879 - +0.6 1,875,946 - -0.1 1,875,990 + -0.2 1,875,990 + -2.0 1,875,998 - -0.3 1,876,047 + -1.0 1,876,096 - +0.9 1,876,173 + -0.1 1,876,173 + -1.2 1,876,181 - -0.6 1,876,245 + MIT1002_01687 0.34 -0.9 1,876,270 + MIT1002_01687 0.44 -2.2 1,876,342 + MIT1002_01687 0.72 +3.4 1,876,394 + +1.2 1,876,402 - +0.8 1,876,409 - -0.4 1,876,457 - +0.4 1,876,537 + +2.2 1,876,537 + +3.0 1,876,538 - +0.2 1,876,545 - -0.6 1,876,686 + MIT1002_01688 0.14 +0.6 1,876,694 - MIT1002_01688 0.15 +0.9 1,876,696 + MIT1002_01688 0.15 -0.6 1,876,699 - MIT1002_01688 0.15 +0.9 1,876,704 - MIT1002_01688 0.16 +0.8 1,876,713 - MIT1002_01688 0.16 -0.5 1,876,731 + MIT1002_01688 0.18 +2.2 1,876,776 + MIT1002_01688 0.22 +0.9 1,876,777 + MIT1002_01688 0.22 -0.7 1,876,777 + MIT1002_01688 0.22 -0.2 1,876,785 - MIT1002_01688 0.23 -0.2 1,876,843 - MIT1002_01688 0.27 +1.4 1,876,863 - MIT1002_01688 0.29 +0.1 1,876,865 + MIT1002_01688 0.29 +0.2
Or see this region's nucleotide sequence