Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01194 and MIT1002_01195 are separated by 219 nucleotides MIT1002_01195 and MIT1002_01196 are separated by 86 nucleotides MIT1002_01196 and MIT1002_01197 are separated by 65 nucleotides
MIT1002_01194: MIT1002_01194 - FMN-dependent NADPH-azoreductase, at 1,309,756 to 1,310,322
_01194
MIT1002_01195: MIT1002_01195 - type IV pilus biogenesis/stability protein PilW, at 1,310,542 to 1,312,500
_01195
MIT1002_01196: MIT1002_01196 - Type III effector pipB2, at 1,312,587 to 1,313,255
_01196
MIT1002_01197: MIT1002_01197 - Quinoprotein ethanol dehydrogenase precursor, at 1,313,321 to 1,315,171
_01197
Position (kb)
1310
1311
1312
1313 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11 at 1309.590 kb on - strand at 1309.605 kb on + strand at 1309.661 kb on - strand at 1309.665 kb on - strand at 1309.692 kb on + strand at 1309.701 kb on - strand at 1309.701 kb on - strand at 1309.728 kb on - strand at 1309.825 kb on + strand, within MIT1002_01194 at 1309.850 kb on + strand, within MIT1002_01194 at 1309.857 kb on + strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.873 kb on - strand, within MIT1002_01194 at 1309.937 kb on + strand, within MIT1002_01194 at 1309.947 kb on - strand, within MIT1002_01194 at 1310.004 kb on - strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.162 kb on - strand, within MIT1002_01194 at 1310.183 kb on + strand, within MIT1002_01194 at 1310.191 kb on - strand, within MIT1002_01194 at 1310.191 kb on - strand, within MIT1002_01194 at 1310.217 kb on + strand, within MIT1002_01194 at 1310.269 kb on - strand at 1310.269 kb on - strand at 1310.386 kb on + strand at 1310.386 kb on + strand at 1310.394 kb on - strand at 1310.394 kb on - strand at 1310.395 kb on + strand at 1310.401 kb on + strand at 1310.446 kb on - strand at 1310.477 kb on + strand at 1310.575 kb on + strand at 1310.596 kb on + strand at 1310.630 kb on + strand at 1310.666 kb on - strand at 1310.801 kb on - strand, within MIT1002_01195 at 1310.801 kb on - strand, within MIT1002_01195 at 1310.838 kb on - strand, within MIT1002_01195 at 1310.968 kb on - strand, within MIT1002_01195 at 1310.978 kb on + strand, within MIT1002_01195 at 1311.044 kb on + strand, within MIT1002_01195 at 1311.117 kb on + strand, within MIT1002_01195 at 1311.125 kb on - strand, within MIT1002_01195 at 1311.189 kb on - strand, within MIT1002_01195 at 1311.189 kb on - strand, within MIT1002_01195 at 1311.274 kb on + strand, within MIT1002_01195 at 1311.275 kb on + strand, within MIT1002_01195 at 1311.283 kb on - strand, within MIT1002_01195 at 1311.317 kb on + strand, within MIT1002_01195 at 1311.325 kb on - strand, within MIT1002_01195 at 1311.325 kb on - strand, within MIT1002_01195 at 1311.353 kb on + strand, within MIT1002_01195 at 1311.416 kb on - strand, within MIT1002_01195 at 1311.476 kb on + strand, within MIT1002_01195 at 1311.498 kb on - strand, within MIT1002_01195 at 1311.505 kb on + strand, within MIT1002_01195 at 1311.505 kb on + strand, within MIT1002_01195 at 1311.559 kb on + strand, within MIT1002_01195 at 1311.598 kb on + strand, within MIT1002_01195 at 1311.604 kb on - strand, within MIT1002_01195 at 1311.708 kb on + strand, within MIT1002_01195 at 1311.716 kb on + strand, within MIT1002_01195 at 1311.935 kb on + strand, within MIT1002_01195 at 1311.949 kb on + strand, within MIT1002_01195 at 1311.981 kb on + strand, within MIT1002_01195 at 1311.982 kb on - strand, within MIT1002_01195 at 1311.998 kb on - strand, within MIT1002_01195 at 1312.066 kb on + strand, within MIT1002_01195 at 1312.121 kb on - strand, within MIT1002_01195 at 1312.164 kb on - strand, within MIT1002_01195 at 1312.166 kb on + strand, within MIT1002_01195 at 1312.166 kb on + strand, within MIT1002_01195 at 1312.174 kb on - strand, within MIT1002_01195 at 1312.244 kb on - strand, within MIT1002_01195 at 1312.373 kb on + strand at 1312.373 kb on - strand at 1312.382 kb on + strand at 1312.402 kb on + strand at 1312.402 kb on + strand at 1312.410 kb on - strand at 1312.410 kb on - strand at 1312.410 kb on - strand at 1312.410 kb on - strand at 1312.412 kb on - strand at 1312.442 kb on - strand at 1312.565 kb on - strand at 1312.670 kb on - strand, within MIT1002_01196 at 1312.677 kb on + strand, within MIT1002_01196 at 1312.743 kb on - strand, within MIT1002_01196 at 1312.764 kb on - strand, within MIT1002_01196 at 1312.788 kb on - strand, within MIT1002_01196 at 1312.795 kb on - strand, within MIT1002_01196 at 1312.802 kb on - strand, within MIT1002_01196 at 1312.862 kb on + strand, within MIT1002_01196 at 1312.901 kb on - strand, within MIT1002_01196 at 1312.922 kb on + strand, within MIT1002_01196 at 1312.930 kb on - strand, within MIT1002_01196 at 1312.932 kb on + strand, within MIT1002_01196 at 1312.932 kb on + strand, within MIT1002_01196 at 1312.940 kb on - strand, within MIT1002_01196 at 1313.005 kb on - strand, within MIT1002_01196 at 1313.022 kb on + strand, within MIT1002_01196 at 1313.030 kb on - strand, within MIT1002_01196 at 1313.051 kb on - strand, within MIT1002_01196 at 1313.057 kb on + strand, within MIT1002_01196 at 1313.057 kb on + strand, within MIT1002_01196 at 1313.057 kb on + strand, within MIT1002_01196 at 1313.057 kb on + strand, within MIT1002_01196 at 1313.060 kb on - strand, within MIT1002_01196 at 1313.065 kb on - strand, within MIT1002_01196 at 1313.065 kb on - strand, within MIT1002_01196 at 1313.082 kb on + strand, within MIT1002_01196 at 1313.082 kb on + strand, within MIT1002_01196 at 1313.082 kb on + strand, within MIT1002_01196 at 1313.082 kb on + strand, within MIT1002_01196 at 1313.090 kb on - strand, within MIT1002_01196 at 1313.090 kb on - strand, within MIT1002_01196 at 1313.095 kb on - strand, within MIT1002_01196 at 1313.095 kb on - strand, within MIT1002_01196 at 1313.097 kb on + strand, within MIT1002_01196 at 1313.105 kb on - strand, within MIT1002_01196 at 1313.105 kb on - strand, within MIT1002_01196 at 1313.105 kb on - strand, within MIT1002_01196 at 1313.120 kb on - strand, within MIT1002_01196 at 1313.194 kb on + strand at 1313.199 kb on + strand at 1313.202 kb on - strand at 1313.228 kb on + strand at 1313.253 kb on + strand at 1313.263 kb on + strand at 1313.263 kb on + strand at 1313.271 kb on - strand at 1313.271 kb on - strand at 1313.271 kb on - strand at 1313.334 kb on - strand at 1313.383 kb on - strand at 1313.405 kb on - strand at 1313.405 kb on - strand at 1313.431 kb on - strand at 1313.434 kb on - strand at 1313.444 kb on + strand at 1313.444 kb on + strand at 1313.444 kb on + strand at 1313.450 kb on - strand at 1313.450 kb on - strand at 1313.452 kb on - strand at 1313.452 kb on - strand at 1313.452 kb on - strand at 1313.452 kb on - strand at 1313.456 kb on + strand at 1313.456 kb on + strand at 1313.492 kb on + strand at 1313.500 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 1,309,590 - +0.4 1,309,605 + +0.1 1,309,661 - +0.6 1,309,665 - -0.4 1,309,692 + -1.0 1,309,701 - +0.5 1,309,701 - -2.4 1,309,728 - +1.4 1,309,825 + MIT1002_01194 0.12 +0.1 1,309,850 + MIT1002_01194 0.17 +0.4 1,309,857 + MIT1002_01194 0.18 +0.2 1,309,858 - MIT1002_01194 0.18 +0.6 1,309,858 - MIT1002_01194 0.18 -0.8 1,309,858 - MIT1002_01194 0.18 -0.4 1,309,858 - MIT1002_01194 0.18 -0.9 1,309,873 - MIT1002_01194 0.21 +0.1 1,309,937 + MIT1002_01194 0.32 +0.4 1,309,947 - MIT1002_01194 0.34 -1.0 1,310,004 - MIT1002_01194 0.44 -0.3 1,310,012 + MIT1002_01194 0.45 +0.3 1,310,012 + MIT1002_01194 0.45 +0.6 1,310,012 + MIT1002_01194 0.45 -0.8 1,310,012 + MIT1002_01194 0.45 -0.2 1,310,012 + MIT1002_01194 0.45 +0.3 1,310,020 - MIT1002_01194 0.47 -0.1 1,310,020 - MIT1002_01194 0.47 -0.6 1,310,020 - MIT1002_01194 0.47 +11.3 1,310,020 - MIT1002_01194 0.47 -0.6 1,310,162 - MIT1002_01194 0.72 -0.7 1,310,183 + MIT1002_01194 0.75 -1.4 1,310,191 - MIT1002_01194 0.77 +0.2 1,310,191 - MIT1002_01194 0.77 -1.3 1,310,217 + MIT1002_01194 0.81 +0.3 1,310,269 - +1.4 1,310,269 - -1.5 1,310,386 + +1.2 1,310,386 + -0.8 1,310,394 - -1.5 1,310,394 - -1.4 1,310,395 + +0.5 1,310,401 + +0.4 1,310,446 - +0.1 1,310,477 + +0.4 1,310,575 + -1.2 1,310,596 + -0.4 1,310,630 + +0.8 1,310,666 - +0.6 1,310,801 - MIT1002_01195 0.13 +0.4 1,310,801 - MIT1002_01195 0.13 -0.7 1,310,838 - MIT1002_01195 0.15 +0.1 1,310,968 - MIT1002_01195 0.22 +0.5 1,310,978 + MIT1002_01195 0.22 -1.1 1,311,044 + MIT1002_01195 0.26 -1.0 1,311,117 + MIT1002_01195 0.29 +1.4 1,311,125 - MIT1002_01195 0.30 +1.3 1,311,189 - MIT1002_01195 0.33 -1.8 1,311,189 - MIT1002_01195 0.33 -0.2 1,311,274 + MIT1002_01195 0.37 +4.5 1,311,275 + MIT1002_01195 0.37 +0.2 1,311,283 - MIT1002_01195 0.38 +0.8 1,311,317 + MIT1002_01195 0.40 -0.4 1,311,325 - MIT1002_01195 0.40 -1.4 1,311,325 - MIT1002_01195 0.40 -0.4 1,311,353 + MIT1002_01195 0.41 -0.4 1,311,416 - MIT1002_01195 0.45 +0.3 1,311,476 + MIT1002_01195 0.48 +0.3 1,311,498 - MIT1002_01195 0.49 +0.6 1,311,505 + MIT1002_01195 0.49 +1.8 1,311,505 + MIT1002_01195 0.49 +0.9 1,311,559 + MIT1002_01195 0.52 -0.1 1,311,598 + MIT1002_01195 0.54 -0.1 1,311,604 - MIT1002_01195 0.54 +0.9 1,311,708 + MIT1002_01195 0.60 -1.7 1,311,716 + MIT1002_01195 0.60 +0.1 1,311,935 + MIT1002_01195 0.71 +0.0 1,311,949 + MIT1002_01195 0.72 +0.2 1,311,981 + MIT1002_01195 0.73 +1.1 1,311,982 - MIT1002_01195 0.74 -1.1 1,311,998 - MIT1002_01195 0.74 -0.9 1,312,066 + MIT1002_01195 0.78 -0.4 1,312,121 - MIT1002_01195 0.81 -1.0 1,312,164 - MIT1002_01195 0.83 -0.1 1,312,166 + MIT1002_01195 0.83 -0.3 1,312,166 + MIT1002_01195 0.83 -0.3 1,312,174 - MIT1002_01195 0.83 -1.1 1,312,244 - MIT1002_01195 0.87 -0.1 1,312,373 + -1.7 1,312,373 - -1.1 1,312,382 + +0.4 1,312,402 + -2.4 1,312,402 + -2.5 1,312,410 - +0.5 1,312,410 - +0.4 1,312,410 - -2.4 1,312,410 - -3.4 1,312,412 - +0.6 1,312,442 - +0.7 1,312,565 - -0.6 1,312,670 - MIT1002_01196 0.12 -0.9 1,312,677 + MIT1002_01196 0.13 +0.8 1,312,743 - MIT1002_01196 0.23 -1.1 1,312,764 - MIT1002_01196 0.26 -3.0 1,312,788 - MIT1002_01196 0.30 -0.0 1,312,795 - MIT1002_01196 0.31 -0.1 1,312,802 - MIT1002_01196 0.32 -1.9 1,312,862 + MIT1002_01196 0.41 -0.6 1,312,901 - MIT1002_01196 0.47 -0.9 1,312,922 + MIT1002_01196 0.50 +0.7 1,312,930 - MIT1002_01196 0.51 +0.4 1,312,932 + MIT1002_01196 0.52 -1.0 1,312,932 + MIT1002_01196 0.52 -0.5 1,312,940 - MIT1002_01196 0.53 -1.6 1,313,005 - MIT1002_01196 0.62 +2.9 1,313,022 + MIT1002_01196 0.65 -1.4 1,313,030 - MIT1002_01196 0.66 -0.8 1,313,051 - MIT1002_01196 0.69 +0.2 1,313,057 + MIT1002_01196 0.70 +0.1 1,313,057 + MIT1002_01196 0.70 -1.5 1,313,057 + MIT1002_01196 0.70 +0.0 1,313,057 + MIT1002_01196 0.70 +0.7 1,313,060 - MIT1002_01196 0.71 +1.5 1,313,065 - MIT1002_01196 0.71 -1.6 1,313,065 - MIT1002_01196 0.71 +0.7 1,313,082 + MIT1002_01196 0.74 +0.1 1,313,082 + MIT1002_01196 0.74 +1.1 1,313,082 + MIT1002_01196 0.74 +0.3 1,313,082 + MIT1002_01196 0.74 -1.0 1,313,090 - MIT1002_01196 0.75 -1.2 1,313,090 - MIT1002_01196 0.75 -1.9 1,313,095 - MIT1002_01196 0.76 -1.3 1,313,095 - MIT1002_01196 0.76 -2.5 1,313,097 + MIT1002_01196 0.76 -1.6 1,313,105 - MIT1002_01196 0.77 -2.1 1,313,105 - MIT1002_01196 0.77 -1.1 1,313,105 - MIT1002_01196 0.77 +1.1 1,313,120 - MIT1002_01196 0.80 -1.8 1,313,194 + -1.1 1,313,199 + +1.3 1,313,202 - -1.8 1,313,228 + -0.7 1,313,253 + +0.8 1,313,263 + -0.7 1,313,263 + +1.6 1,313,271 - +0.2 1,313,271 - -0.0 1,313,271 - -1.1 1,313,334 - +0.5 1,313,383 - +1.9 1,313,405 - -0.2 1,313,405 - -0.7 1,313,431 - +1.1 1,313,434 - +0.8 1,313,444 + +1.8 1,313,444 + -0.7 1,313,444 + +0.2 1,313,450 - -0.3 1,313,450 - -1.9 1,313,452 - -2.7 1,313,452 - +1.6 1,313,452 - +0.3 1,313,452 - -0.9 1,313,456 + -0.2 1,313,456 + -0.0 1,313,492 + +0.2 1,313,500 - -0.0
Or see this region's nucleotide sequence