Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00756 and MIT1002_00757 are separated by 176 nucleotides MIT1002_00757 and MIT1002_00758 are separated by 13 nucleotides MIT1002_00758 and MIT1002_00759 are separated by 2 nucleotides MIT1002_00759 and MIT1002_00760 are separated by 32 nucleotides MIT1002_00760 and MIT1002_00761 are separated by 3 nucleotides
MIT1002_00756: MIT1002_00756 - TonB-dependent receptor, at 830,599 to 833,247
_00756
MIT1002_00757: MIT1002_00757 - hypothetical protein, at 833,424 to 834,182
_00757
MIT1002_00758: MIT1002_00758 - Biopolymer transport protein ExbB, at 834,196 to 835,560
_00758
MIT1002_00759: MIT1002_00759 - protein TolQ, at 835,563 to 836,081
_00759
MIT1002_00760: MIT1002_00760 - Biopolymer transport protein ExbD, at 836,114 to 836,521
_00760
MIT1002_00761: MIT1002_00761 - transport protein TonB, at 836,525 to 837,133
_00761
Position (kb) 834
835
836 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 833.208 kb on + strand at 833.208 kb on + strand at 833.215 kb on + strand at 833.215 kb on - strand at 833.216 kb on + strand at 833.223 kb on - strand at 833.223 kb on - strand at 833.223 kb on - strand at 833.224 kb on - strand at 833.241 kb on + strand at 833.249 kb on + strand at 833.268 kb on + strand at 833.413 kb on + strand at 833.422 kb on + strand at 833.439 kb on + strand at 833.534 kb on + strand, within MIT1002_00757 at 833.561 kb on + strand, within MIT1002_00757 at 833.569 kb on + strand, within MIT1002_00757 at 833.601 kb on - strand, within MIT1002_00757 at 833.603 kb on - strand, within MIT1002_00757 at 833.611 kb on - strand, within MIT1002_00757 at 833.611 kb on - strand, within MIT1002_00757 at 833.632 kb on + strand, within MIT1002_00757 at 833.632 kb on + strand, within MIT1002_00757 at 833.632 kb on + strand, within MIT1002_00757 at 833.640 kb on - strand, within MIT1002_00757 at 833.640 kb on - strand, within MIT1002_00757 at 833.640 kb on - strand, within MIT1002_00757 at 833.647 kb on - strand, within MIT1002_00757 at 833.722 kb on + strand, within MIT1002_00757 at 833.727 kb on - strand, within MIT1002_00757 at 833.746 kb on + strand, within MIT1002_00757 at 833.756 kb on - strand, within MIT1002_00757 at 833.770 kb on + strand, within MIT1002_00757 at 833.770 kb on + strand, within MIT1002_00757 at 833.770 kb on + strand, within MIT1002_00757 at 833.778 kb on - strand, within MIT1002_00757 at 833.780 kb on - strand, within MIT1002_00757 at 833.783 kb on - strand, within MIT1002_00757 at 833.845 kb on + strand, within MIT1002_00757 at 833.847 kb on + strand, within MIT1002_00757 at 833.847 kb on + strand, within MIT1002_00757 at 833.847 kb on + strand, within MIT1002_00757 at 833.847 kb on + strand, within MIT1002_00757 at 833.847 kb on + strand, within MIT1002_00757 at 833.855 kb on - strand, within MIT1002_00757 at 833.857 kb on + strand, within MIT1002_00757 at 833.898 kb on - strand, within MIT1002_00757 at 833.934 kb on + strand, within MIT1002_00757 at 833.940 kb on - strand, within MIT1002_00757 at 833.942 kb on - strand, within MIT1002_00757 at 834.017 kb on - strand, within MIT1002_00757 at 834.038 kb on + strand, within MIT1002_00757 at 834.078 kb on + strand, within MIT1002_00757 at 834.177 kb on + strand at 834.206 kb on - strand at 834.226 kb on + strand at 834.283 kb on + strand at 834.374 kb on + strand, within MIT1002_00758 at 834.387 kb on - strand, within MIT1002_00758 at 834.444 kb on + strand, within MIT1002_00758 at 834.444 kb on + strand, within MIT1002_00758 at 834.444 kb on + strand, within MIT1002_00758 at 834.484 kb on + strand, within MIT1002_00758 at 834.486 kb on + strand, within MIT1002_00758 at 834.496 kb on + strand, within MIT1002_00758 at 834.508 kb on + strand, within MIT1002_00758 at 834.508 kb on + strand, within MIT1002_00758 at 834.528 kb on - strand, within MIT1002_00758 at 834.544 kb on - strand, within MIT1002_00758 at 834.582 kb on - strand, within MIT1002_00758 at 834.642 kb on - strand, within MIT1002_00758 at 834.648 kb on - strand, within MIT1002_00758 at 834.738 kb on + strand, within MIT1002_00758 at 834.746 kb on - strand, within MIT1002_00758 at 834.746 kb on - strand, within MIT1002_00758 at 834.785 kb on - strand, within MIT1002_00758 at 834.835 kb on + strand, within MIT1002_00758 at 834.835 kb on + strand, within MIT1002_00758 at 834.843 kb on - strand, within MIT1002_00758 at 834.869 kb on - strand, within MIT1002_00758 at 834.942 kb on + strand, within MIT1002_00758 at 834.950 kb on - strand, within MIT1002_00758 at 834.961 kb on - strand, within MIT1002_00758 at 834.977 kb on + strand, within MIT1002_00758 at 834.985 kb on - strand, within MIT1002_00758 at 834.998 kb on + strand, within MIT1002_00758 at 834.998 kb on + strand, within MIT1002_00758 at 835.000 kb on + strand, within MIT1002_00758 at 835.000 kb on + strand, within MIT1002_00758 at 835.000 kb on + strand, within MIT1002_00758 at 835.000 kb on + strand, within MIT1002_00758 at 835.010 kb on + strand, within MIT1002_00758 at 835.010 kb on + strand, within MIT1002_00758 at 835.010 kb on + strand, within MIT1002_00758 at 835.015 kb on + strand, within MIT1002_00758 at 835.015 kb on + strand, within MIT1002_00758 at 835.018 kb on - strand, within MIT1002_00758 at 835.018 kb on - strand, within MIT1002_00758 at 835.028 kb on - strand, within MIT1002_00758 at 835.076 kb on + strand, within MIT1002_00758 at 835.117 kb on - strand, within MIT1002_00758 at 835.134 kb on + strand, within MIT1002_00758 at 835.316 kb on - strand, within MIT1002_00758 at 835.341 kb on + strand, within MIT1002_00758 at 835.341 kb on + strand, within MIT1002_00758 at 835.349 kb on - strand, within MIT1002_00758 at 835.349 kb on - strand, within MIT1002_00758 at 835.376 kb on - strand, within MIT1002_00758 at 835.376 kb on - strand, within MIT1002_00758 at 835.437 kb on - strand at 835.464 kb on + strand at 835.472 kb on - strand at 835.472 kb on - strand at 835.472 kb on - strand at 835.472 kb on - strand at 835.476 kb on + strand at 835.477 kb on - strand at 835.477 kb on - strand at 835.485 kb on - strand at 835.509 kb on - strand at 835.555 kb on + strand at 835.562 kb on - strand at 835.614 kb on + strand at 835.630 kb on - strand, within MIT1002_00759 at 835.659 kb on + strand, within MIT1002_00759 at 835.734 kb on - strand, within MIT1002_00759 at 835.865 kb on - strand, within MIT1002_00759 at 835.922 kb on - strand, within MIT1002_00759 at 835.928 kb on - strand, within MIT1002_00759 at 835.937 kb on + strand, within MIT1002_00759 at 836.057 kb on - strand at 836.057 kb on - strand at 836.084 kb on - strand at 836.092 kb on + strand at 836.100 kb on - strand at 836.249 kb on - strand, within MIT1002_00760 at 836.256 kb on + strand, within MIT1002_00760 at 836.363 kb on + strand, within MIT1002_00760 at 836.382 kb on - strand, within MIT1002_00760 at 836.404 kb on + strand, within MIT1002_00760 at 836.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 833,208 + -1.5 833,208 + -3.2 833,215 + -1.6 833,215 - -2.9 833,216 + -5.6 833,223 - -0.6 833,223 - -0.8 833,223 - -4.5 833,224 - -6.4 833,241 + +0.0 833,249 + +2.0 833,268 + +0.8 833,413 + -0.2 833,422 + -2.5 833,439 + -4.7 833,534 + MIT1002_00757 0.14 -6.0 833,561 + MIT1002_00757 0.18 -1.3 833,569 + MIT1002_00757 0.19 -0.8 833,601 - MIT1002_00757 0.23 -3.6 833,603 - MIT1002_00757 0.24 -0.8 833,611 - MIT1002_00757 0.25 -1.3 833,611 - MIT1002_00757 0.25 +1.2 833,632 + MIT1002_00757 0.27 -3.5 833,632 + MIT1002_00757 0.27 -0.5 833,632 + MIT1002_00757 0.27 -0.7 833,640 - MIT1002_00757 0.28 -1.5 833,640 - MIT1002_00757 0.28 -0.2 833,640 - MIT1002_00757 0.28 -1.4 833,647 - MIT1002_00757 0.29 -0.6 833,722 + MIT1002_00757 0.39 -0.3 833,727 - MIT1002_00757 0.40 -4.3 833,746 + MIT1002_00757 0.42 -3.8 833,756 - MIT1002_00757 0.44 -1.1 833,770 + MIT1002_00757 0.46 -3.7 833,770 + MIT1002_00757 0.46 -2.0 833,770 + MIT1002_00757 0.46 -2.2 833,778 - MIT1002_00757 0.47 -1.8 833,780 - MIT1002_00757 0.47 -2.1 833,783 - MIT1002_00757 0.47 -1.6 833,845 + MIT1002_00757 0.55 +0.2 833,847 + MIT1002_00757 0.56 -1.6 833,847 + MIT1002_00757 0.56 -3.5 833,847 + MIT1002_00757 0.56 -2.0 833,847 + MIT1002_00757 0.56 +0.2 833,847 + MIT1002_00757 0.56 -1.7 833,855 - MIT1002_00757 0.57 -1.7 833,857 + MIT1002_00757 0.57 -2.5 833,898 - MIT1002_00757 0.62 -0.8 833,934 + MIT1002_00757 0.67 -3.2 833,940 - MIT1002_00757 0.68 -2.8 833,942 - MIT1002_00757 0.68 -2.0 834,017 - MIT1002_00757 0.78 -0.3 834,038 + MIT1002_00757 0.81 -5.8 834,078 + MIT1002_00757 0.86 -1.3 834,177 + -1.8 834,206 - -0.6 834,226 + -2.2 834,283 + -1.0 834,374 + MIT1002_00758 0.13 -2.8 834,387 - MIT1002_00758 0.14 -1.3 834,444 + MIT1002_00758 0.18 -2.1 834,444 + MIT1002_00758 0.18 -3.0 834,444 + MIT1002_00758 0.18 -2.6 834,484 + MIT1002_00758 0.21 -4.0 834,486 + MIT1002_00758 0.21 -2.0 834,496 + MIT1002_00758 0.22 -1.6 834,508 + MIT1002_00758 0.23 -0.7 834,508 + MIT1002_00758 0.23 -2.1 834,528 - MIT1002_00758 0.24 -2.6 834,544 - MIT1002_00758 0.25 -2.0 834,582 - MIT1002_00758 0.28 -1.4 834,642 - MIT1002_00758 0.33 -2.0 834,648 - MIT1002_00758 0.33 -1.6 834,738 + MIT1002_00758 0.40 -1.9 834,746 - MIT1002_00758 0.40 -0.3 834,746 - MIT1002_00758 0.40 -2.3 834,785 - MIT1002_00758 0.43 -2.7 834,835 + MIT1002_00758 0.47 -1.2 834,835 + MIT1002_00758 0.47 -1.1 834,843 - MIT1002_00758 0.47 -1.4 834,869 - MIT1002_00758 0.49 -3.4 834,942 + MIT1002_00758 0.55 -0.3 834,950 - MIT1002_00758 0.55 -2.0 834,961 - MIT1002_00758 0.56 -1.5 834,977 + MIT1002_00758 0.57 -1.6 834,985 - MIT1002_00758 0.58 -1.9 834,998 + MIT1002_00758 0.59 +1.2 834,998 + MIT1002_00758 0.59 -0.9 835,000 + MIT1002_00758 0.59 -1.8 835,000 + MIT1002_00758 0.59 +0.0 835,000 + MIT1002_00758 0.59 -1.7 835,000 + MIT1002_00758 0.59 -4.6 835,010 + MIT1002_00758 0.60 -4.0 835,010 + MIT1002_00758 0.60 -0.6 835,010 + MIT1002_00758 0.60 -2.2 835,015 + MIT1002_00758 0.60 -2.2 835,015 + MIT1002_00758 0.60 -3.8 835,018 - MIT1002_00758 0.60 +1.5 835,018 - MIT1002_00758 0.60 -2.2 835,028 - MIT1002_00758 0.61 -1.0 835,076 + MIT1002_00758 0.64 -2.3 835,117 - MIT1002_00758 0.67 -3.1 835,134 + MIT1002_00758 0.69 -2.2 835,316 - MIT1002_00758 0.82 -1.4 835,341 + MIT1002_00758 0.84 -4.2 835,341 + MIT1002_00758 0.84 -5.1 835,349 - MIT1002_00758 0.84 -1.7 835,349 - MIT1002_00758 0.84 -1.2 835,376 - MIT1002_00758 0.86 -1.9 835,376 - MIT1002_00758 0.86 -2.0 835,437 - -3.6 835,464 + -1.2 835,472 - -5.1 835,472 - +0.3 835,472 - -2.9 835,472 - +1.5 835,476 + -3.9 835,477 - -7.1 835,477 - -0.8 835,485 - -1.9 835,509 - -1.8 835,555 + -2.7 835,562 - -2.7 835,614 + -6.6 835,630 - MIT1002_00759 0.13 -2.5 835,659 + MIT1002_00759 0.18 -0.9 835,734 - MIT1002_00759 0.33 -1.9 835,865 - MIT1002_00759 0.58 -5.8 835,922 - MIT1002_00759 0.69 -1.5 835,928 - MIT1002_00759 0.70 -0.9 835,937 + MIT1002_00759 0.72 -1.2 836,057 - -2.1 836,057 - -2.3 836,084 - -0.9 836,092 + -0.7 836,100 - -2.3 836,249 - MIT1002_00760 0.33 -2.2 836,256 + MIT1002_00760 0.35 -3.3 836,363 + MIT1002_00760 0.61 -2.3 836,382 - MIT1002_00760 0.66 -2.1 836,404 + MIT1002_00760 0.71 -0.9 836,515 - -3.0
Or see this region's nucleotide sequence