Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00455 and MIT1002_00456 are separated by 311 nucleotides MIT1002_00456 and MIT1002_00457 are separated by 202 nucleotides
MIT1002_00455: MIT1002_00455 - NAD-dependent malic enzyme, at 497,720 to 499,444
_00455
MIT1002_00456: MIT1002_00456 - hypothetical protein, at 499,756 to 500,742
_00456
MIT1002_00457: MIT1002_00457 - Disulfide-bond oxidoreductase YghU, at 500,945 to 501,802
_00457
Position (kb) 499
500
501 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 498.785 kb on - strand, within MIT1002_00455 at 498.827 kb on + strand, within MIT1002_00455 at 498.936 kb on + strand, within MIT1002_00455 at 498.994 kb on + strand, within MIT1002_00455 at 499.000 kb on + strand, within MIT1002_00455 at 499.028 kb on + strand, within MIT1002_00455 at 499.081 kb on + strand, within MIT1002_00455 at 499.081 kb on + strand, within MIT1002_00455 at 499.086 kb on + strand, within MIT1002_00455 at 499.089 kb on - strand, within MIT1002_00455 at 499.161 kb on - strand, within MIT1002_00455 at 499.172 kb on - strand, within MIT1002_00455 at 499.246 kb on + strand, within MIT1002_00455 at 499.251 kb on + strand, within MIT1002_00455 at 499.254 kb on - strand, within MIT1002_00455 at 499.256 kb on + strand, within MIT1002_00455 at 499.264 kb on - strand, within MIT1002_00455 at 499.264 kb on - strand, within MIT1002_00455 at 499.264 kb on - strand, within MIT1002_00455 at 499.269 kb on - strand, within MIT1002_00455 at 499.324 kb on + strand at 499.324 kb on + strand at 499.324 kb on + strand at 499.324 kb on + strand at 499.332 kb on - strand at 499.332 kb on - strand at 499.391 kb on - strand at 499.412 kb on + strand at 499.455 kb on + strand at 499.470 kb on + strand at 499.478 kb on - strand at 499.483 kb on + strand at 499.503 kb on + strand at 499.534 kb on + strand at 499.552 kb on - strand at 499.578 kb on - strand at 499.749 kb on - strand at 499.755 kb on - strand at 499.887 kb on - strand, within MIT1002_00456 at 499.897 kb on - strand, within MIT1002_00456 at 499.899 kb on + strand, within MIT1002_00456 at 499.905 kb on - strand, within MIT1002_00456 at 499.934 kb on + strand, within MIT1002_00456 at 499.945 kb on - strand, within MIT1002_00456 at 500.076 kb on + strand, within MIT1002_00456 at 500.076 kb on - strand, within MIT1002_00456 at 500.248 kb on + strand, within MIT1002_00456 at 500.256 kb on - strand, within MIT1002_00456 at 500.364 kb on - strand, within MIT1002_00456 at 500.372 kb on - strand, within MIT1002_00456 at 500.374 kb on + strand, within MIT1002_00456 at 500.377 kb on - strand, within MIT1002_00456 at 500.382 kb on - strand, within MIT1002_00456 at 500.458 kb on + strand, within MIT1002_00456 at 500.458 kb on + strand, within MIT1002_00456 at 500.466 kb on - strand, within MIT1002_00456 at 500.508 kb on - strand, within MIT1002_00456 at 500.513 kb on - strand, within MIT1002_00456 at 500.525 kb on + strand, within MIT1002_00456 at 500.542 kb on + strand, within MIT1002_00456 at 500.586 kb on + strand, within MIT1002_00456 at 500.592 kb on - strand, within MIT1002_00456 at 500.663 kb on - strand at 500.687 kb on + strand at 500.732 kb on + strand at 500.740 kb on - strand at 500.772 kb on - strand at 500.779 kb on + strand at 500.867 kb on - strand at 500.881 kb on + strand at 500.906 kb on + strand at 500.906 kb on + strand at 500.909 kb on - strand at 500.936 kb on - strand at 501.026 kb on - strand at 501.032 kb on + strand, within MIT1002_00457 at 501.040 kb on - strand, within MIT1002_00457 at 501.085 kb on + strand, within MIT1002_00457 at 501.086 kb on - strand, within MIT1002_00457 at 501.131 kb on + strand, within MIT1002_00457 at 501.152 kb on + strand, within MIT1002_00457 at 501.152 kb on + strand, within MIT1002_00457 at 501.189 kb on + strand, within MIT1002_00457 at 501.298 kb on - strand, within MIT1002_00457 at 501.434 kb on - strand, within MIT1002_00457 at 501.436 kb on + strand, within MIT1002_00457 at 501.444 kb on + strand, within MIT1002_00457 at 501.444 kb on - strand, within MIT1002_00457 at 501.452 kb on - strand, within MIT1002_00457 at 501.520 kb on - strand, within MIT1002_00457 at 501.541 kb on + strand, within MIT1002_00457 at 501.570 kb on - strand, within MIT1002_00457 at 501.638 kb on - strand, within MIT1002_00457 at 501.645 kb on - strand, within MIT1002_00457 at 501.652 kb on - strand, within MIT1002_00457 at 501.689 kb on - strand, within MIT1002_00457 at 501.707 kb on - strand, within MIT1002_00457 at 501.727 kb on + strand at 501.727 kb on + strand at 501.727 kb on + strand at 501.727 kb on + strand at 501.735 kb on - strand at 501.735 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 498,785 - MIT1002_00455 0.62 -1.8 498,827 + MIT1002_00455 0.64 +1.7 498,936 + MIT1002_00455 0.70 -1.4 498,994 + MIT1002_00455 0.74 -0.0 499,000 + MIT1002_00455 0.74 -0.9 499,028 + MIT1002_00455 0.76 -3.4 499,081 + MIT1002_00455 0.79 +1.0 499,081 + MIT1002_00455 0.79 +0.6 499,086 + MIT1002_00455 0.79 -0.1 499,089 - MIT1002_00455 0.79 +0.7 499,161 - MIT1002_00455 0.84 -0.8 499,172 - MIT1002_00455 0.84 -0.8 499,246 + MIT1002_00455 0.88 +1.8 499,251 + MIT1002_00455 0.89 +3.8 499,254 - MIT1002_00455 0.89 -2.5 499,256 + MIT1002_00455 0.89 +1.0 499,264 - MIT1002_00455 0.90 -0.9 499,264 - MIT1002_00455 0.90 +0.6 499,264 - MIT1002_00455 0.90 +0.0 499,269 - MIT1002_00455 0.90 -0.1 499,324 + -1.7 499,324 + -0.1 499,324 + -1.6 499,324 + +0.0 499,332 - -0.4 499,332 - +0.7 499,391 - -1.9 499,412 + -0.7 499,455 + +2.8 499,470 + -0.5 499,478 - +0.9 499,483 + -0.5 499,503 + +1.0 499,534 + -0.4 499,552 - +1.3 499,578 - -0.8 499,749 - -0.3 499,755 - +0.0 499,887 - MIT1002_00456 0.13 -0.1 499,897 - MIT1002_00456 0.14 +1.7 499,899 + MIT1002_00456 0.14 -1.1 499,905 - MIT1002_00456 0.15 -0.8 499,934 + MIT1002_00456 0.18 +1.4 499,945 - MIT1002_00456 0.19 -2.2 500,076 + MIT1002_00456 0.32 +1.8 500,076 - MIT1002_00456 0.32 -0.0 500,248 + MIT1002_00456 0.50 +0.2 500,256 - MIT1002_00456 0.51 -0.2 500,364 - MIT1002_00456 0.62 -1.1 500,372 - MIT1002_00456 0.62 -0.8 500,374 + MIT1002_00456 0.63 -0.7 500,377 - MIT1002_00456 0.63 +0.2 500,382 - MIT1002_00456 0.63 +1.6 500,458 + MIT1002_00456 0.71 +0.6 500,458 + MIT1002_00456 0.71 -0.5 500,466 - MIT1002_00456 0.72 -0.6 500,508 - MIT1002_00456 0.76 +0.2 500,513 - MIT1002_00456 0.77 -0.9 500,525 + MIT1002_00456 0.78 +1.7 500,542 + MIT1002_00456 0.80 -0.5 500,586 + MIT1002_00456 0.84 -0.0 500,592 - MIT1002_00456 0.85 -1.0 500,663 - -1.4 500,687 + +1.8 500,732 + +0.7 500,740 - -2.7 500,772 - +0.1 500,779 + -1.8 500,867 - +1.4 500,881 + +2.0 500,906 + -0.0 500,906 + +0.2 500,909 - +1.6 500,936 - +0.8 501,026 - +2.6 501,032 + MIT1002_00457 0.10 +0.6 501,040 - MIT1002_00457 0.11 +1.6 501,085 + MIT1002_00457 0.16 +0.2 501,086 - MIT1002_00457 0.16 -0.6 501,131 + MIT1002_00457 0.22 -0.0 501,152 + MIT1002_00457 0.24 -2.9 501,152 + MIT1002_00457 0.24 -0.9 501,189 + MIT1002_00457 0.28 +2.0 501,298 - MIT1002_00457 0.41 -0.9 501,434 - MIT1002_00457 0.57 +0.4 501,436 + MIT1002_00457 0.57 -0.3 501,444 + MIT1002_00457 0.58 +0.2 501,444 - MIT1002_00457 0.58 +1.2 501,452 - MIT1002_00457 0.59 -0.2 501,520 - MIT1002_00457 0.67 -1.2 501,541 + MIT1002_00457 0.69 +0.1 501,570 - MIT1002_00457 0.73 +1.4 501,638 - MIT1002_00457 0.81 -0.1 501,645 - MIT1002_00457 0.82 +1.1 501,652 - MIT1002_00457 0.82 -0.5 501,689 - MIT1002_00457 0.87 +0.5 501,707 - MIT1002_00457 0.89 -1.1 501,727 + +1.2 501,727 + +1.1 501,727 + +0.9 501,727 + -0.9 501,735 - -0.1 501,735 - -0.1
Or see this region's nucleotide sequence