Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00153 and MIT1002_00154 are separated by 463 nucleotides MIT1002_00154 and MIT1002_00155 overlap by 8 nucleotides
MIT1002_00153: MIT1002_00153 - DNA helicase II, at 168,162 to 170,333
_00153
MIT1002_00154: MIT1002_00154 - Serine acetyltransferase, at 170,797 to 171,609
_00154
MIT1002_00155: MIT1002_00155 - Diguanylate cyclase DosC, at 171,602 to 173,110
_00155
Position (kb)
170
171
172 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 169.875 kb on - strand, within MIT1002_00153 at 169.877 kb on - strand, within MIT1002_00153 at 169.929 kb on - strand, within MIT1002_00153 at 169.948 kb on - strand, within MIT1002_00153 at 170.087 kb on - strand, within MIT1002_00153 at 170.116 kb on - strand at 170.154 kb on - strand at 170.209 kb on - strand at 170.226 kb on + strand at 170.284 kb on + strand at 170.326 kb on - strand at 170.335 kb on - strand at 170.343 kb on - strand at 170.357 kb on + strand at 170.365 kb on - strand at 170.367 kb on - strand at 170.400 kb on + strand at 170.400 kb on + strand at 170.493 kb on + strand at 170.502 kb on + strand at 170.510 kb on - strand at 170.536 kb on + strand at 170.544 kb on - strand at 170.574 kb on + strand at 170.600 kb on - strand at 170.717 kb on - strand at 170.793 kb on + strand at 170.894 kb on + strand, within MIT1002_00154 at 170.919 kb on - strand, within MIT1002_00154 at 170.936 kb on + strand, within MIT1002_00154 at 170.944 kb on - strand, within MIT1002_00154 at 171.033 kb on - strand, within MIT1002_00154 at 171.133 kb on + strand, within MIT1002_00154 at 171.138 kb on - strand, within MIT1002_00154 at 171.139 kb on - strand, within MIT1002_00154 at 171.281 kb on + strand, within MIT1002_00154 at 171.299 kb on + strand, within MIT1002_00154 at 171.315 kb on - strand, within MIT1002_00154 at 171.335 kb on + strand, within MIT1002_00154 at 171.343 kb on - strand, within MIT1002_00154 at 171.371 kb on + strand, within MIT1002_00154 at 171.449 kb on + strand, within MIT1002_00154 at 171.470 kb on + strand, within MIT1002_00154 at 171.475 kb on + strand, within MIT1002_00154 at 171.618 kb on - strand at 171.667 kb on + strand at 171.667 kb on + strand at 171.675 kb on - strand at 171.684 kb on + strand at 171.697 kb on + strand at 171.697 kb on + strand at 171.697 kb on + strand at 171.697 kb on + strand at 171.705 kb on - strand at 171.705 kb on - strand at 171.705 kb on - strand at 171.705 kb on - strand at 171.818 kb on - strand, within MIT1002_00155 at 171.876 kb on - strand, within MIT1002_00155 at 171.911 kb on + strand, within MIT1002_00155 at 172.006 kb on - strand, within MIT1002_00155 at 172.035 kb on - strand, within MIT1002_00155 at 172.035 kb on - strand, within MIT1002_00155 at 172.035 kb on - strand, within MIT1002_00155 at 172.051 kb on - strand, within MIT1002_00155 at 172.054 kb on + strand, within MIT1002_00155 at 172.054 kb on + strand, within MIT1002_00155 at 172.062 kb on - strand, within MIT1002_00155 at 172.092 kb on + strand, within MIT1002_00155 at 172.100 kb on - strand, within MIT1002_00155 at 172.101 kb on + strand, within MIT1002_00155 at 172.148 kb on + strand, within MIT1002_00155 at 172.156 kb on + strand, within MIT1002_00155 at 172.169 kb on - strand, within MIT1002_00155 at 172.171 kb on - strand, within MIT1002_00155 at 172.196 kb on + strand, within MIT1002_00155 at 172.238 kb on + strand, within MIT1002_00155 at 172.238 kb on + strand, within MIT1002_00155 at 172.238 kb on + strand, within MIT1002_00155 at 172.238 kb on + strand, within MIT1002_00155 at 172.238 kb on + strand, within MIT1002_00155 at 172.238 kb on + strand, within MIT1002_00155 at 172.240 kb on + strand, within MIT1002_00155 at 172.246 kb on - strand, within MIT1002_00155 at 172.246 kb on - strand, within MIT1002_00155 at 172.246 kb on - strand, within MIT1002_00155 at 172.246 kb on - strand, within MIT1002_00155 at 172.246 kb on - strand, within MIT1002_00155 at 172.275 kb on + strand, within MIT1002_00155 at 172.275 kb on + strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.283 kb on - strand, within MIT1002_00155 at 172.285 kb on + strand, within MIT1002_00155 at 172.285 kb on + strand, within MIT1002_00155 at 172.293 kb on - strand, within MIT1002_00155 at 172.335 kb on + strand, within MIT1002_00155 at 172.338 kb on - strand, within MIT1002_00155 at 172.364 kb on - strand, within MIT1002_00155 at 172.366 kb on + strand, within MIT1002_00155 at 172.486 kb on + strand, within MIT1002_00155 at 172.496 kb on + strand, within MIT1002_00155 at 172.498 kb on + strand, within MIT1002_00155 at 172.506 kb on - strand, within MIT1002_00155 at 172.506 kb on - strand, within MIT1002_00155 at 172.507 kb on - strand, within MIT1002_00155
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 169,875 - MIT1002_00153 0.79 +0.4 169,877 - MIT1002_00153 0.79 -0.1 169,929 - MIT1002_00153 0.81 +1.7 169,948 - MIT1002_00153 0.82 +0.2 170,087 - MIT1002_00153 0.89 +1.3 170,116 - -0.5 170,154 - +0.1 170,209 - +0.1 170,226 + +1.3 170,284 + -0.2 170,326 - -0.7 170,335 - +1.4 170,343 - -0.5 170,357 + -0.2 170,365 - +0.4 170,367 - -0.0 170,400 + -1.4 170,400 + -0.2 170,493 + -1.2 170,502 + -1.4 170,510 - -3.5 170,536 + +0.4 170,544 - +1.0 170,574 + -1.5 170,600 - +0.4 170,717 - -3.1 170,793 + -0.7 170,894 + MIT1002_00154 0.12 -2.7 170,919 - MIT1002_00154 0.15 -1.1 170,936 + MIT1002_00154 0.17 -0.8 170,944 - MIT1002_00154 0.18 -1.7 171,033 - MIT1002_00154 0.29 -3.0 171,133 + MIT1002_00154 0.41 -5.5 171,138 - MIT1002_00154 0.42 +0.0 171,139 - MIT1002_00154 0.42 -3.2 171,281 + MIT1002_00154 0.60 -2.3 171,299 + MIT1002_00154 0.62 -1.7 171,315 - MIT1002_00154 0.64 -0.5 171,335 + MIT1002_00154 0.66 -2.9 171,343 - MIT1002_00154 0.67 +1.6 171,371 + MIT1002_00154 0.71 -0.7 171,449 + MIT1002_00154 0.80 -2.0 171,470 + MIT1002_00154 0.83 -2.1 171,475 + MIT1002_00154 0.83 -3.2 171,618 - -1.1 171,667 + +0.8 171,667 + -0.5 171,675 - -0.5 171,684 + +0.3 171,697 + +0.9 171,697 + -0.3 171,697 + -0.4 171,697 + +2.2 171,705 - +0.4 171,705 - -0.7 171,705 - +1.1 171,705 - +1.5 171,818 - MIT1002_00155 0.14 -0.3 171,876 - MIT1002_00155 0.18 +1.3 171,911 + MIT1002_00155 0.20 +0.9 172,006 - MIT1002_00155 0.27 +0.0 172,035 - MIT1002_00155 0.29 -0.5 172,035 - MIT1002_00155 0.29 +0.0 172,035 - MIT1002_00155 0.29 +1.6 172,051 - MIT1002_00155 0.30 +2.3 172,054 + MIT1002_00155 0.30 +0.5 172,054 + MIT1002_00155 0.30 -0.9 172,062 - MIT1002_00155 0.30 -2.3 172,092 + MIT1002_00155 0.32 +0.8 172,100 - MIT1002_00155 0.33 +1.1 172,101 + MIT1002_00155 0.33 -0.6 172,148 + MIT1002_00155 0.36 +0.2 172,156 + MIT1002_00155 0.37 +0.5 172,169 - MIT1002_00155 0.38 -0.3 172,171 - MIT1002_00155 0.38 -2.4 172,196 + MIT1002_00155 0.39 +0.5 172,238 + MIT1002_00155 0.42 +0.7 172,238 + MIT1002_00155 0.42 -1.2 172,238 + MIT1002_00155 0.42 +0.1 172,238 + MIT1002_00155 0.42 -0.5 172,238 + MIT1002_00155 0.42 +0.6 172,238 + MIT1002_00155 0.42 -0.3 172,240 + MIT1002_00155 0.42 -2.2 172,246 - MIT1002_00155 0.43 -0.3 172,246 - MIT1002_00155 0.43 +0.3 172,246 - MIT1002_00155 0.43 -1.3 172,246 - MIT1002_00155 0.43 +0.6 172,246 - MIT1002_00155 0.43 +0.3 172,275 + MIT1002_00155 0.45 +1.4 172,275 + MIT1002_00155 0.45 -0.9 172,283 - MIT1002_00155 0.45 +0.0 172,283 - MIT1002_00155 0.45 -1.4 172,283 - MIT1002_00155 0.45 +1.9 172,283 - MIT1002_00155 0.45 -0.6 172,283 - MIT1002_00155 0.45 -2.1 172,283 - MIT1002_00155 0.45 +0.5 172,283 - MIT1002_00155 0.45 -0.5 172,283 - MIT1002_00155 0.45 +0.8 172,283 - MIT1002_00155 0.45 +0.1 172,283 - MIT1002_00155 0.45 -0.2 172,285 + MIT1002_00155 0.45 +1.6 172,285 + MIT1002_00155 0.45 +1.8 172,293 - MIT1002_00155 0.46 -0.3 172,335 + MIT1002_00155 0.49 -1.4 172,338 - MIT1002_00155 0.49 +1.4 172,364 - MIT1002_00155 0.50 -0.6 172,366 + MIT1002_00155 0.51 +1.0 172,486 + MIT1002_00155 0.59 +1.3 172,496 + MIT1002_00155 0.59 -0.3 172,498 + MIT1002_00155 0.59 +1.3 172,506 - MIT1002_00155 0.60 -2.7 172,506 - MIT1002_00155 0.60 -1.2 172,507 - MIT1002_00155 0.60 -2.1
Or see this region's nucleotide sequence