Experiment: monoculture; Experiment C, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00108 and MIT1002_00109 are separated by 24 nucleotides MIT1002_00109 and MIT1002_00110 are separated by 3 nucleotides MIT1002_00110 and MIT1002_00111 are separated by 194 nucleotides
MIT1002_00108: MIT1002_00108 - Bifunctional (p)ppGpp synthase/hydrolase SpoT, at 115,970 to 118,081
_00108
MIT1002_00109: MIT1002_00109 - Putative reactive intermediate deaminase TdcF, at 118,106 to 118,492
_00109
MIT1002_00110: MIT1002_00110 - tRNA (guanosine(18)-2'-O)-methyltransferase, at 118,496 to 119,203
_00110
MIT1002_00111: MIT1002_00111 - ATP-dependent DNA helicase RecG, at 119,398 to 121,470
_00111
Position (kb)
118
119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 117.421 kb on - strand, within MIT1002_00108 at 117.426 kb on - strand, within MIT1002_00108 at 117.446 kb on + strand, within MIT1002_00108 at 117.446 kb on + strand, within MIT1002_00108 at 117.456 kb on + strand, within MIT1002_00108 at 117.456 kb on + strand, within MIT1002_00108 at 117.464 kb on - strand, within MIT1002_00108 at 117.464 kb on - strand, within MIT1002_00108 at 117.502 kb on + strand, within MIT1002_00108 at 117.551 kb on - strand, within MIT1002_00108 at 117.572 kb on + strand, within MIT1002_00108 at 117.598 kb on + strand, within MIT1002_00108 at 117.713 kb on + strand, within MIT1002_00108 at 117.723 kb on - strand, within MIT1002_00108 at 117.728 kb on - strand, within MIT1002_00108 at 117.811 kb on - strand, within MIT1002_00108 at 117.842 kb on - strand, within MIT1002_00108 at 117.908 kb on - strand at 117.960 kb on - strand at 118.032 kb on - strand at 118.037 kb on - strand at 118.052 kb on - strand at 118.108 kb on + strand at 118.116 kb on + strand at 118.116 kb on - strand at 118.279 kb on + strand, within MIT1002_00109 at 118.287 kb on - strand, within MIT1002_00109 at 118.287 kb on - strand, within MIT1002_00109 at 118.287 kb on - strand, within MIT1002_00109 at 118.287 kb on - strand, within MIT1002_00109 at 118.289 kb on + strand, within MIT1002_00109 at 118.291 kb on + strand, within MIT1002_00109 at 118.321 kb on - strand, within MIT1002_00109 at 118.402 kb on + strand, within MIT1002_00109 at 118.402 kb on + strand, within MIT1002_00109 at 118.402 kb on + strand, within MIT1002_00109 at 118.402 kb on + strand, within MIT1002_00109 at 118.404 kb on - strand, within MIT1002_00109 at 118.404 kb on - strand, within MIT1002_00109 at 118.410 kb on - strand, within MIT1002_00109 at 118.410 kb on - strand, within MIT1002_00109 at 118.410 kb on - strand, within MIT1002_00109 at 118.410 kb on - strand, within MIT1002_00109 at 118.410 kb on - strand, within MIT1002_00109 at 118.410 kb on - strand, within MIT1002_00109 at 118.421 kb on + strand, within MIT1002_00109 at 118.432 kb on - strand, within MIT1002_00109 at 118.439 kb on - strand, within MIT1002_00109 at 118.441 kb on + strand, within MIT1002_00109 at 118.441 kb on + strand, within MIT1002_00109 at 118.441 kb on + strand, within MIT1002_00109 at 118.441 kb on + strand, within MIT1002_00109 at 118.441 kb on + strand, within MIT1002_00109 at 118.441 kb on + strand, within MIT1002_00109 at 118.441 kb on + strand, within MIT1002_00109 at 118.449 kb on - strand, within MIT1002_00109 at 118.449 kb on - strand, within MIT1002_00109 at 118.449 kb on - strand, within MIT1002_00109 at 118.449 kb on - strand, within MIT1002_00109 at 118.449 kb on - strand, within MIT1002_00109 at 118.604 kb on - strand, within MIT1002_00110 at 118.644 kb on - strand, within MIT1002_00110 at 118.680 kb on + strand, within MIT1002_00110 at 118.682 kb on + strand, within MIT1002_00110 at 118.685 kb on - strand, within MIT1002_00110 at 118.690 kb on - strand, within MIT1002_00110 at 118.692 kb on + strand, within MIT1002_00110 at 118.760 kb on + strand, within MIT1002_00110 at 118.780 kb on + strand, within MIT1002_00110 at 118.822 kb on + strand, within MIT1002_00110 at 118.822 kb on + strand, within MIT1002_00110 at 118.825 kb on - strand, within MIT1002_00110 at 118.876 kb on + strand, within MIT1002_00110 at 118.900 kb on - strand, within MIT1002_00110 at 118.918 kb on + strand, within MIT1002_00110 at 119.002 kb on + strand, within MIT1002_00110 at 119.002 kb on + strand, within MIT1002_00110 at 119.002 kb on + strand, within MIT1002_00110 at 119.002 kb on + strand, within MIT1002_00110 at 119.002 kb on + strand, within MIT1002_00110 at 119.002 kb on + strand, within MIT1002_00110 at 119.010 kb on - strand, within MIT1002_00110 at 119.010 kb on - strand, within MIT1002_00110 at 119.010 kb on - strand, within MIT1002_00110 at 119.032 kb on - strand, within MIT1002_00110 at 119.035 kb on - strand, within MIT1002_00110 at 119.039 kb on + strand, within MIT1002_00110 at 119.042 kb on - strand, within MIT1002_00110 at 119.078 kb on - strand, within MIT1002_00110 at 119.086 kb on + strand, within MIT1002_00110 at 119.093 kb on + strand, within MIT1002_00110 at 119.093 kb on + strand, within MIT1002_00110 at 119.093 kb on + strand, within MIT1002_00110 at 119.101 kb on - strand, within MIT1002_00110 at 119.204 kb on + strand at 119.212 kb on - strand at 119.251 kb on - strand at 119.253 kb on - strand at 119.257 kb on + strand at 119.284 kb on + strand at 119.292 kb on - strand at 119.326 kb on + strand at 119.426 kb on - strand at 119.444 kb on + strand at 119.444 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 5 remove 117,421 - MIT1002_00108 0.69 +0.9 117,426 - MIT1002_00108 0.69 -1.2 117,446 + MIT1002_00108 0.70 -1.3 117,446 + MIT1002_00108 0.70 +1.0 117,456 + MIT1002_00108 0.70 -0.0 117,456 + MIT1002_00108 0.70 +1.3 117,464 - MIT1002_00108 0.71 -1.1 117,464 - MIT1002_00108 0.71 +1.6 117,502 + MIT1002_00108 0.73 +0.6 117,551 - MIT1002_00108 0.75 -1.2 117,572 + MIT1002_00108 0.76 -1.5 117,598 + MIT1002_00108 0.77 -1.7 117,713 + MIT1002_00108 0.83 -1.1 117,723 - MIT1002_00108 0.83 -1.3 117,728 - MIT1002_00108 0.83 +0.1 117,811 - MIT1002_00108 0.87 -1.9 117,842 - MIT1002_00108 0.89 +1.3 117,908 - -0.5 117,960 - -1.9 118,032 - -1.5 118,037 - -1.4 118,052 - -2.2 118,108 + -2.2 118,116 + +0.4 118,116 - -0.7 118,279 + MIT1002_00109 0.45 -2.4 118,287 - MIT1002_00109 0.47 -1.0 118,287 - MIT1002_00109 0.47 -0.9 118,287 - MIT1002_00109 0.47 -3.2 118,287 - MIT1002_00109 0.47 -1.8 118,289 + MIT1002_00109 0.47 -3.0 118,291 + MIT1002_00109 0.48 -1.4 118,321 - MIT1002_00109 0.56 -1.3 118,402 + MIT1002_00109 0.76 -0.3 118,402 + MIT1002_00109 0.76 -1.8 118,402 + MIT1002_00109 0.76 -1.9 118,402 + MIT1002_00109 0.76 -0.5 118,404 - MIT1002_00109 0.77 +0.1 118,404 - MIT1002_00109 0.77 +1.1 118,410 - MIT1002_00109 0.79 -2.6 118,410 - MIT1002_00109 0.79 -2.7 118,410 - MIT1002_00109 0.79 -2.2 118,410 - MIT1002_00109 0.79 -3.4 118,410 - MIT1002_00109 0.79 -1.7 118,410 - MIT1002_00109 0.79 -0.2 118,421 + MIT1002_00109 0.81 -0.4 118,432 - MIT1002_00109 0.84 -1.8 118,439 - MIT1002_00109 0.86 -2.1 118,441 + MIT1002_00109 0.87 -1.2 118,441 + MIT1002_00109 0.87 -0.1 118,441 + MIT1002_00109 0.87 -1.9 118,441 + MIT1002_00109 0.87 -2.8 118,441 + MIT1002_00109 0.87 -3.1 118,441 + MIT1002_00109 0.87 -2.1 118,441 + MIT1002_00109 0.87 -0.2 118,449 - MIT1002_00109 0.89 -3.0 118,449 - MIT1002_00109 0.89 -1.7 118,449 - MIT1002_00109 0.89 -0.5 118,449 - MIT1002_00109 0.89 +0.2 118,449 - MIT1002_00109 0.89 -3.5 118,604 - MIT1002_00110 0.15 -0.7 118,644 - MIT1002_00110 0.21 +0.1 118,680 + MIT1002_00110 0.26 -0.3 118,682 + MIT1002_00110 0.26 -1.9 118,685 - MIT1002_00110 0.27 -0.5 118,690 - MIT1002_00110 0.27 +0.6 118,692 + MIT1002_00110 0.28 -0.3 118,760 + MIT1002_00110 0.37 -0.2 118,780 + MIT1002_00110 0.40 +1.6 118,822 + MIT1002_00110 0.46 -0.4 118,822 + MIT1002_00110 0.46 -0.5 118,825 - MIT1002_00110 0.46 -1.0 118,876 + MIT1002_00110 0.54 -0.8 118,900 - MIT1002_00110 0.57 -0.7 118,918 + MIT1002_00110 0.60 +0.3 119,002 + MIT1002_00110 0.71 +1.3 119,002 + MIT1002_00110 0.71 +0.3 119,002 + MIT1002_00110 0.71 +0.4 119,002 + MIT1002_00110 0.71 -0.3 119,002 + MIT1002_00110 0.71 +0.5 119,002 + MIT1002_00110 0.71 +1.1 119,010 - MIT1002_00110 0.73 -0.6 119,010 - MIT1002_00110 0.73 -0.3 119,010 - MIT1002_00110 0.73 +2.6 119,032 - MIT1002_00110 0.76 -1.2 119,035 - MIT1002_00110 0.76 -1.5 119,039 + MIT1002_00110 0.77 -0.1 119,042 - MIT1002_00110 0.77 -0.7 119,078 - MIT1002_00110 0.82 +0.1 119,086 + MIT1002_00110 0.83 -1.2 119,093 + MIT1002_00110 0.84 -1.3 119,093 + MIT1002_00110 0.84 +1.0 119,093 + MIT1002_00110 0.84 +0.7 119,101 - MIT1002_00110 0.85 +0.4 119,204 + -2.3 119,212 - -0.9 119,251 - -0.1 119,253 - +0.9 119,257 + +1.4 119,284 + -1.8 119,292 - -1.2 119,326 + -1.7 119,426 - -2.3 119,444 + +0.3 119,444 + +0.1
Or see this region's nucleotide sequence