Experiment: monoculture; Experiment C, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_04158 and MIT1002_04159 are separated by 152 nucleotides MIT1002_04159 and MIT1002_04160 are separated by 198 nucleotides MIT1002_04160 and MIT1002_04161 are separated by 261 nucleotides MIT1002_04161 and MIT1002_04162 overlap by 14 nucleotides MIT1002_04162 and MIT1002_04163 are separated by 129 nucleotides
MIT1002_04158: MIT1002_04158 - putative secretion ATPase, PEP-CTERM locus subfamily, at 25,799 to 26,770
_04158
MIT1002_04159: MIT1002_04159 - hypothetical protein, at 26,923 to 27,270
_04159
MIT1002_04160: MIT1002_04160 - hypothetical protein, at 27,469 to 28,086
_04160
MIT1002_04161: MIT1002_04161 - hypothetical protein, at 28,348 to 28,524
_04161
MIT1002_04162: MIT1002_04162 - hypothetical protein, at 28,511 to 28,813
_04162
MIT1002_04163: MIT1002_04163 - hypothetical protein, at 28,943 to 29,203
_04163
Position (kb) 27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 26.488 kb on - strand, within MIT1002_04158 at 26.498 kb on - strand, within MIT1002_04158 at 26.505 kb on - strand, within MIT1002_04158 at 26.698 kb on + strand at 26.706 kb on + strand at 26.714 kb on - strand at 26.714 kb on - strand at 26.714 kb on - strand at 26.714 kb on - strand at 26.756 kb on + strand at 26.756 kb on + strand at 26.785 kb on + strand at 26.811 kb on + strand at 26.811 kb on + strand at 26.811 kb on + strand at 26.811 kb on + strand at 26.823 kb on - strand at 26.896 kb on - strand at 26.966 kb on - strand, within MIT1002_04159 at 27.005 kb on + strand, within MIT1002_04159 at 27.040 kb on + strand, within MIT1002_04159 at 27.050 kb on + strand, within MIT1002_04159 at 27.058 kb on + strand, within MIT1002_04159 at 27.058 kb on - strand, within MIT1002_04159 at 27.121 kb on + strand, within MIT1002_04159 at 27.187 kb on + strand, within MIT1002_04159 at 27.221 kb on + strand, within MIT1002_04159 at 27.229 kb on - strand, within MIT1002_04159 at 27.277 kb on + strand at 27.387 kb on + strand at 27.395 kb on + strand at 27.395 kb on - strand at 27.424 kb on + strand at 27.427 kb on + strand at 27.432 kb on - strand at 27.466 kb on + strand at 27.523 kb on + strand at 27.538 kb on - strand, within MIT1002_04160 at 27.543 kb on - strand, within MIT1002_04160 at 27.557 kb on - strand, within MIT1002_04160 at 27.651 kb on + strand, within MIT1002_04160 at 27.651 kb on + strand, within MIT1002_04160 at 27.664 kb on + strand, within MIT1002_04160 at 27.693 kb on - strand, within MIT1002_04160 at 27.714 kb on - strand, within MIT1002_04160 at 27.718 kb on + strand, within MIT1002_04160 at 27.726 kb on - strand, within MIT1002_04160 at 27.746 kb on + strand, within MIT1002_04160 at 27.752 kb on + strand, within MIT1002_04160 at 27.754 kb on - strand, within MIT1002_04160 at 27.759 kb on - strand, within MIT1002_04160 at 27.774 kb on - strand, within MIT1002_04160 at 27.847 kb on - strand, within MIT1002_04160 at 27.958 kb on + strand, within MIT1002_04160 at 28.007 kb on - strand, within MIT1002_04160 at 28.051 kb on - strand at 28.114 kb on + strand at 28.140 kb on - strand at 28.158 kb on - strand at 28.194 kb on - strand at 28.196 kb on + strand at 28.209 kb on - strand at 28.284 kb on - strand at 28.324 kb on + strand at 28.328 kb on - strand at 28.354 kb on - strand at 28.424 kb on + strand, within MIT1002_04161 at 28.429 kb on - strand, within MIT1002_04161 at 28.459 kb on + strand, within MIT1002_04161 at 28.460 kb on + strand, within MIT1002_04161 at 28.460 kb on + strand, within MIT1002_04161 at 28.625 kb on + strand, within MIT1002_04162 at 28.684 kb on + strand, within MIT1002_04162 at 28.699 kb on + strand, within MIT1002_04162 at 28.699 kb on - strand, within MIT1002_04162 at 28.751 kb on + strand, within MIT1002_04162 at 28.785 kb on + strand at 28.794 kb on - strand at 28.794 kb on - strand at 28.852 kb on + strand at 28.852 kb on - strand at 28.889 kb on + strand at 28.889 kb on - strand at 28.949 kb on - strand at 28.960 kb on + strand at 29.011 kb on - strand, within MIT1002_04163
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 4 remove 26,488 - MIT1002_04158 0.71 -1.5 26,498 - MIT1002_04158 0.72 -1.4 26,505 - MIT1002_04158 0.73 +1.0 26,698 + +0.7 26,706 + -0.1 26,714 - +0.1 26,714 - -0.2 26,714 - -0.1 26,714 - +1.0 26,756 + +1.1 26,756 + -1.7 26,785 + -0.3 26,811 + +0.9 26,811 + -0.7 26,811 + -0.0 26,811 + +0.5 26,823 - -0.6 26,896 - -1.4 26,966 - MIT1002_04159 0.12 +0.9 27,005 + MIT1002_04159 0.24 +0.9 27,040 + MIT1002_04159 0.34 +0.4 27,050 + MIT1002_04159 0.36 -2.7 27,058 + MIT1002_04159 0.39 +2.2 27,058 - MIT1002_04159 0.39 -0.0 27,121 + MIT1002_04159 0.57 -1.2 27,187 + MIT1002_04159 0.76 +0.1 27,221 + MIT1002_04159 0.86 +0.5 27,229 - MIT1002_04159 0.88 +1.1 27,277 + +0.2 27,387 + +0.1 27,395 + +0.3 27,395 - -0.9 27,424 + +0.2 27,427 + -1.4 27,432 - -0.2 27,466 + -1.8 27,523 + +0.3 27,538 - MIT1002_04160 0.11 -0.7 27,543 - MIT1002_04160 0.12 -1.1 27,557 - MIT1002_04160 0.14 +0.1 27,651 + MIT1002_04160 0.29 -0.5 27,651 + MIT1002_04160 0.29 -1.2 27,664 + MIT1002_04160 0.32 -1.7 27,693 - MIT1002_04160 0.36 +0.6 27,714 - MIT1002_04160 0.40 +0.4 27,718 + MIT1002_04160 0.40 +0.2 27,726 - MIT1002_04160 0.42 +0.1 27,746 + MIT1002_04160 0.45 -1.1 27,752 + MIT1002_04160 0.46 -1.3 27,754 - MIT1002_04160 0.46 +0.8 27,759 - MIT1002_04160 0.47 -0.5 27,774 - MIT1002_04160 0.49 -0.4 27,847 - MIT1002_04160 0.61 +0.3 27,958 + MIT1002_04160 0.79 +0.4 28,007 - MIT1002_04160 0.87 -0.1 28,051 - +0.3 28,114 + -0.4 28,140 - -1.1 28,158 - -0.1 28,194 - +0.1 28,196 + -1.9 28,209 - -0.5 28,284 - -1.1 28,324 + -0.2 28,328 - -0.1 28,354 - -0.3 28,424 + MIT1002_04161 0.43 +0.7 28,429 - MIT1002_04161 0.46 -0.1 28,459 + MIT1002_04161 0.63 +1.1 28,460 + MIT1002_04161 0.63 +0.3 28,460 + MIT1002_04161 0.63 +0.6 28,625 + MIT1002_04162 0.38 +1.2 28,684 + MIT1002_04162 0.57 -1.3 28,699 + MIT1002_04162 0.62 +0.9 28,699 - MIT1002_04162 0.62 -0.0 28,751 + MIT1002_04162 0.79 -1.1 28,785 + +0.7 28,794 - -0.3 28,794 - -0.3 28,852 + -0.8 28,852 - -0.8 28,889 + -0.1 28,889 - +0.7 28,949 - -3.2 28,960 + +0.6 29,011 - MIT1002_04163 0.26 -0.7
Or see this region's nucleotide sequence