Experiment: monoculture; Experiment C, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01021 and MIT1002_01022 are separated by 263 nucleotides MIT1002_01022 and MIT1002_01023 are separated by 75 nucleotides MIT1002_01023 and MIT1002_01024 are separated by 317 nucleotides
MIT1002_01021: MIT1002_01021 - putative diguanylate cyclase YedQ, at 1,131,481 to 1,132,380
_01021
MIT1002_01022: MIT1002_01022 - NEM-activable K(+)/H(+) antiporter, at 1,132,644 to 1,134,611
_01022
MIT1002_01023: MIT1002_01023 - hypothetical protein, at 1,134,687 to 1,134,920
_01023
MIT1002_01024: MIT1002_01024 - tRNA-specific adenosine deaminase, at 1,135,238 to 1,135,861
_01024
Position (kb)
1132
1133
1134
1135 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1131.660 kb on - strand, within MIT1002_01021 at 1131.662 kb on + strand, within MIT1002_01021 at 1131.662 kb on + strand, within MIT1002_01021 at 1131.665 kb on - strand, within MIT1002_01021 at 1131.670 kb on - strand, within MIT1002_01021 at 1131.692 kb on - strand, within MIT1002_01021 at 1131.709 kb on - strand, within MIT1002_01021 at 1131.855 kb on - strand, within MIT1002_01021 at 1131.928 kb on + strand, within MIT1002_01021 at 1131.928 kb on + strand, within MIT1002_01021 at 1131.971 kb on - strand, within MIT1002_01021 at 1132.139 kb on - strand, within MIT1002_01021 at 1132.139 kb on - strand, within MIT1002_01021 at 1132.153 kb on + strand, within MIT1002_01021 at 1132.153 kb on + strand, within MIT1002_01021 at 1132.161 kb on - strand, within MIT1002_01021 at 1132.161 kb on - strand, within MIT1002_01021 at 1132.192 kb on + strand, within MIT1002_01021 at 1132.318 kb on + strand at 1132.318 kb on + strand at 1132.326 kb on - strand at 1132.352 kb on - strand at 1132.354 kb on + strand at 1132.354 kb on + strand at 1132.390 kb on - strand at 1132.432 kb on + strand at 1132.818 kb on + strand at 1132.916 kb on - strand, within MIT1002_01022 at 1132.938 kb on + strand, within MIT1002_01022 at 1132.946 kb on - strand, within MIT1002_01022 at 1132.948 kb on - strand, within MIT1002_01022 at 1132.956 kb on - strand, within MIT1002_01022 at 1133.025 kb on + strand, within MIT1002_01022 at 1133.041 kb on + strand, within MIT1002_01022 at 1133.068 kb on + strand, within MIT1002_01022 at 1133.115 kb on - strand, within MIT1002_01022 at 1133.147 kb on + strand, within MIT1002_01022 at 1133.170 kb on - strand, within MIT1002_01022 at 1133.177 kb on + strand, within MIT1002_01022 at 1133.185 kb on - strand, within MIT1002_01022 at 1133.195 kb on + strand, within MIT1002_01022 at 1133.195 kb on + strand, within MIT1002_01022 at 1133.203 kb on - strand, within MIT1002_01022 at 1133.203 kb on - strand, within MIT1002_01022 at 1133.203 kb on - strand, within MIT1002_01022 at 1133.243 kb on - strand, within MIT1002_01022 at 1133.243 kb on - strand, within MIT1002_01022 at 1133.250 kb on + strand, within MIT1002_01022 at 1133.284 kb on - strand, within MIT1002_01022 at 1133.298 kb on + strand, within MIT1002_01022 at 1133.324 kb on + strand, within MIT1002_01022 at 1133.393 kb on - strand, within MIT1002_01022 at 1133.428 kb on - strand, within MIT1002_01022 at 1133.481 kb on + strand, within MIT1002_01022 at 1133.496 kb on - strand, within MIT1002_01022 at 1133.505 kb on + strand, within MIT1002_01022 at 1133.505 kb on - strand, within MIT1002_01022 at 1133.513 kb on - strand, within MIT1002_01022 at 1133.626 kb on + strand, within MIT1002_01022 at 1133.627 kb on + strand, within MIT1002_01022 at 1133.634 kb on - strand, within MIT1002_01022 at 1133.698 kb on + strand, within MIT1002_01022 at 1133.730 kb on + strand, within MIT1002_01022 at 1133.730 kb on + strand, within MIT1002_01022 at 1133.730 kb on + strand, within MIT1002_01022 at 1133.744 kb on + strand, within MIT1002_01022 at 1133.748 kb on - strand, within MIT1002_01022 at 1133.752 kb on - strand, within MIT1002_01022 at 1133.752 kb on - strand, within MIT1002_01022 at 1133.754 kb on - strand, within MIT1002_01022 at 1133.846 kb on + strand, within MIT1002_01022 at 1133.954 kb on + strand, within MIT1002_01022 at 1133.961 kb on + strand, within MIT1002_01022 at 1133.971 kb on + strand, within MIT1002_01022 at 1133.996 kb on - strand, within MIT1002_01022 at 1134.095 kb on + strand, within MIT1002_01022 at 1134.133 kb on + strand, within MIT1002_01022 at 1134.135 kb on + strand, within MIT1002_01022 at 1134.143 kb on - strand, within MIT1002_01022 at 1134.171 kb on + strand, within MIT1002_01022 at 1134.179 kb on - strand, within MIT1002_01022 at 1134.179 kb on - strand, within MIT1002_01022 at 1134.256 kb on - strand, within MIT1002_01022 at 1134.313 kb on + strand, within MIT1002_01022 at 1134.336 kb on - strand, within MIT1002_01022 at 1134.351 kb on + strand, within MIT1002_01022 at 1134.386 kb on + strand, within MIT1002_01022 at 1134.404 kb on + strand, within MIT1002_01022 at 1134.535 kb on + strand at 1134.558 kb on + strand at 1134.616 kb on + strand at 1134.616 kb on + strand at 1134.618 kb on + strand at 1134.624 kb on - strand at 1134.626 kb on - strand at 1134.626 kb on - strand at 1134.684 kb on + strand at 1134.684 kb on + strand at 1134.687 kb on - strand at 1134.756 kb on - strand, within MIT1002_01023 at 1134.848 kb on + strand, within MIT1002_01023 at 1135.113 kb on - strand at 1135.290 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 4 remove 1,131,660 - MIT1002_01021 0.20 +1.3 1,131,662 + MIT1002_01021 0.20 -1.7 1,131,662 + MIT1002_01021 0.20 +0.5 1,131,665 - MIT1002_01021 0.20 -0.2 1,131,670 - MIT1002_01021 0.21 -1.0 1,131,692 - MIT1002_01021 0.23 -0.1 1,131,709 - MIT1002_01021 0.25 +1.1 1,131,855 - MIT1002_01021 0.42 +0.3 1,131,928 + MIT1002_01021 0.50 -0.5 1,131,928 + MIT1002_01021 0.50 +0.7 1,131,971 - MIT1002_01021 0.54 -0.6 1,132,139 - MIT1002_01021 0.73 -1.2 1,132,139 - MIT1002_01021 0.73 +0.8 1,132,153 + MIT1002_01021 0.75 -1.1 1,132,153 + MIT1002_01021 0.75 -0.8 1,132,161 - MIT1002_01021 0.76 -0.3 1,132,161 - MIT1002_01021 0.76 +0.4 1,132,192 + MIT1002_01021 0.79 -0.5 1,132,318 + +0.1 1,132,318 + -0.0 1,132,326 - -0.9 1,132,352 - +0.2 1,132,354 + -0.8 1,132,354 + +0.5 1,132,390 - +0.1 1,132,432 + -1.0 1,132,818 + +0.2 1,132,916 - MIT1002_01022 0.14 +0.7 1,132,938 + MIT1002_01022 0.15 -0.2 1,132,946 - MIT1002_01022 0.15 -0.6 1,132,948 - MIT1002_01022 0.15 +0.0 1,132,956 - MIT1002_01022 0.16 +0.7 1,133,025 + MIT1002_01022 0.19 +1.7 1,133,041 + MIT1002_01022 0.20 +0.6 1,133,068 + MIT1002_01022 0.22 +1.1 1,133,115 - MIT1002_01022 0.24 +1.2 1,133,147 + MIT1002_01022 0.26 +0.0 1,133,170 - MIT1002_01022 0.27 +0.3 1,133,177 + MIT1002_01022 0.27 +1.0 1,133,185 - MIT1002_01022 0.27 +0.4 1,133,195 + MIT1002_01022 0.28 +0.5 1,133,195 + MIT1002_01022 0.28 +0.5 1,133,203 - MIT1002_01022 0.28 +0.7 1,133,203 - MIT1002_01022 0.28 -0.6 1,133,203 - MIT1002_01022 0.28 +0.5 1,133,243 - MIT1002_01022 0.30 +2.9 1,133,243 - MIT1002_01022 0.30 -0.1 1,133,250 + MIT1002_01022 0.31 -0.3 1,133,284 - MIT1002_01022 0.33 -0.6 1,133,298 + MIT1002_01022 0.33 +3.0 1,133,324 + MIT1002_01022 0.35 -0.4 1,133,393 - MIT1002_01022 0.38 -1.3 1,133,428 - MIT1002_01022 0.40 -0.2 1,133,481 + MIT1002_01022 0.43 -0.2 1,133,496 - MIT1002_01022 0.43 +0.8 1,133,505 + MIT1002_01022 0.44 +0.5 1,133,505 - MIT1002_01022 0.44 -1.5 1,133,513 - MIT1002_01022 0.44 +1.5 1,133,626 + MIT1002_01022 0.50 +1.9 1,133,627 + MIT1002_01022 0.50 +1.2 1,133,634 - MIT1002_01022 0.50 +0.5 1,133,698 + MIT1002_01022 0.54 +0.7 1,133,730 + MIT1002_01022 0.55 -2.2 1,133,730 + MIT1002_01022 0.55 -2.9 1,133,730 + MIT1002_01022 0.55 +0.8 1,133,744 + MIT1002_01022 0.56 +0.4 1,133,748 - MIT1002_01022 0.56 +0.8 1,133,752 - MIT1002_01022 0.56 +0.4 1,133,752 - MIT1002_01022 0.56 +0.3 1,133,754 - MIT1002_01022 0.56 +0.8 1,133,846 + MIT1002_01022 0.61 -0.1 1,133,954 + MIT1002_01022 0.67 +1.7 1,133,961 + MIT1002_01022 0.67 -2.2 1,133,971 + MIT1002_01022 0.67 +0.2 1,133,996 - MIT1002_01022 0.69 +1.1 1,134,095 + MIT1002_01022 0.74 +0.8 1,134,133 + MIT1002_01022 0.76 +0.5 1,134,135 + MIT1002_01022 0.76 +0.4 1,134,143 - MIT1002_01022 0.76 +0.9 1,134,171 + MIT1002_01022 0.78 -0.7 1,134,179 - MIT1002_01022 0.78 -0.2 1,134,179 - MIT1002_01022 0.78 -0.8 1,134,256 - MIT1002_01022 0.82 +1.5 1,134,313 + MIT1002_01022 0.85 +0.0 1,134,336 - MIT1002_01022 0.86 +1.6 1,134,351 + MIT1002_01022 0.87 -0.4 1,134,386 + MIT1002_01022 0.89 -0.7 1,134,404 + MIT1002_01022 0.89 +0.2 1,134,535 + +1.9 1,134,558 + +0.7 1,134,616 + +0.3 1,134,616 + +2.2 1,134,618 + +1.1 1,134,624 - +0.3 1,134,626 - -1.2 1,134,626 - -1.0 1,134,684 + -0.3 1,134,684 + +0.2 1,134,687 - -0.3 1,134,756 - MIT1002_01023 0.29 +0.7 1,134,848 + MIT1002_01023 0.69 +2.5 1,135,113 - +1.3 1,135,290 + -1.7
Or see this region's nucleotide sequence