Experiment: monoculture; Experiment C, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00846 and MIT1002_00847 are separated by 42 nucleotides MIT1002_00847 and MIT1002_00848 are separated by 239 nucleotides MIT1002_00848 and MIT1002_00849 are separated by 85 nucleotides MIT1002_00849 and MIT1002_00850 are separated by 161 nucleotides
MIT1002_00846: MIT1002_00846 - Major NAD(P)H-flavin oxidoreductase, at 927,328 to 927,984
_00846
MIT1002_00847: MIT1002_00847 - hypothetical protein, at 928,027 to 928,302
_00847
MIT1002_00848: MIT1002_00848 - hypothetical protein, at 928,542 to 928,868
_00848
MIT1002_00849: MIT1002_00849 - bifunctional preprotein translocase subunit SecD/SecF, at 928,954 to 931,521
_00849
MIT1002_00850: MIT1002_00850 - potassium/proton antiporter, at 931,683 to 933,146
_00850
Position (kb) 928
929
930
931
932 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 927.979 kb on + strand at 928.017 kb on - strand at 928.020 kb on + strand at 928.088 kb on + strand, within MIT1002_00847 at 928.184 kb on + strand, within MIT1002_00847 at 928.192 kb on - strand, within MIT1002_00847 at 928.192 kb on - strand, within MIT1002_00847 at 928.195 kb on - strand, within MIT1002_00847 at 928.312 kb on + strand at 928.326 kb on - strand at 928.419 kb on + strand at 928.443 kb on - strand at 928.451 kb on - strand at 928.451 kb on - strand at 928.453 kb on - strand at 928.569 kb on - strand at 928.626 kb on - strand, within MIT1002_00848 at 928.629 kb on + strand, within MIT1002_00848 at 928.637 kb on - strand, within MIT1002_00848 at 928.637 kb on - strand, within MIT1002_00848 at 928.645 kb on - strand, within MIT1002_00848 at 928.670 kb on + strand, within MIT1002_00848 at 928.729 kb on + strand, within MIT1002_00848 at 928.784 kb on + strand, within MIT1002_00848 at 928.784 kb on + strand, within MIT1002_00848 at 928.784 kb on + strand, within MIT1002_00848 at 928.792 kb on - strand, within MIT1002_00848 at 928.801 kb on + strand, within MIT1002_00848 at 928.803 kb on + strand, within MIT1002_00848 at 928.803 kb on + strand, within MIT1002_00848 at 928.804 kb on - strand, within MIT1002_00848 at 928.818 kb on + strand, within MIT1002_00848 at 928.846 kb on + strand at 928.858 kb on - strand at 928.859 kb on - strand at 928.860 kb on - strand at 929.060 kb on - strand at 929.072 kb on - strand at 929.083 kb on - strand at 929.093 kb on - strand at 929.111 kb on + strand at 929.201 kb on + strand at 929.209 kb on - strand at 929.209 kb on - strand at 929.270 kb on + strand, within MIT1002_00849 at 929.280 kb on + strand, within MIT1002_00849 at 929.293 kb on - strand, within MIT1002_00849 at 929.293 kb on - strand, within MIT1002_00849 at 929.305 kb on + strand, within MIT1002_00849 at 929.313 kb on - strand, within MIT1002_00849 at 929.345 kb on + strand, within MIT1002_00849 at 929.353 kb on - strand, within MIT1002_00849 at 929.366 kb on + strand, within MIT1002_00849 at 929.381 kb on + strand, within MIT1002_00849 at 929.381 kb on + strand, within MIT1002_00849 at 929.389 kb on - strand, within MIT1002_00849 at 929.389 kb on - strand, within MIT1002_00849 at 929.389 kb on - strand, within MIT1002_00849 at 929.411 kb on - strand, within MIT1002_00849 at 929.436 kb on + strand, within MIT1002_00849 at 929.444 kb on + strand, within MIT1002_00849 at 929.472 kb on - strand, within MIT1002_00849 at 929.482 kb on - strand, within MIT1002_00849 at 929.543 kb on + strand, within MIT1002_00849 at 929.582 kb on - strand, within MIT1002_00849 at 929.628 kb on + strand, within MIT1002_00849 at 929.636 kb on - strand, within MIT1002_00849 at 929.652 kb on + strand, within MIT1002_00849 at 929.660 kb on - strand, within MIT1002_00849 at 929.661 kb on - strand, within MIT1002_00849 at 929.719 kb on + strand, within MIT1002_00849 at 929.727 kb on - strand, within MIT1002_00849 at 929.776 kb on + strand, within MIT1002_00849 at 929.784 kb on + strand, within MIT1002_00849 at 929.833 kb on - strand, within MIT1002_00849 at 929.860 kb on + strand, within MIT1002_00849 at 929.925 kb on + strand, within MIT1002_00849 at 929.984 kb on - strand, within MIT1002_00849 at 930.004 kb on + strand, within MIT1002_00849 at 930.022 kb on + strand, within MIT1002_00849 at 930.136 kb on + strand, within MIT1002_00849 at 930.136 kb on + strand, within MIT1002_00849 at 930.160 kb on - strand, within MIT1002_00849 at 930.170 kb on - strand, within MIT1002_00849 at 930.172 kb on + strand, within MIT1002_00849 at 930.180 kb on - strand, within MIT1002_00849 at 930.180 kb on - strand, within MIT1002_00849 at 930.180 kb on - strand, within MIT1002_00849 at 930.204 kb on - strand, within MIT1002_00849 at 930.204 kb on - strand, within MIT1002_00849 at 930.290 kb on + strand, within MIT1002_00849 at 930.298 kb on - strand, within MIT1002_00849 at 930.298 kb on - strand, within MIT1002_00849 at 930.392 kb on + strand, within MIT1002_00849 at 930.394 kb on + strand, within MIT1002_00849 at 930.394 kb on + strand, within MIT1002_00849 at 930.394 kb on + strand, within MIT1002_00849 at 930.402 kb on - strand, within MIT1002_00849 at 930.402 kb on - strand, within MIT1002_00849 at 930.402 kb on - strand, within MIT1002_00849 at 930.402 kb on - strand, within MIT1002_00849 at 930.424 kb on + strand, within MIT1002_00849 at 930.432 kb on - strand, within MIT1002_00849 at 930.441 kb on + strand, within MIT1002_00849 at 930.456 kb on - strand, within MIT1002_00849 at 930.473 kb on + strand, within MIT1002_00849 at 930.503 kb on - strand, within MIT1002_00849 at 930.503 kb on - strand, within MIT1002_00849 at 930.525 kb on + strand, within MIT1002_00849 at 930.532 kb on + strand, within MIT1002_00849 at 930.533 kb on - strand, within MIT1002_00849 at 930.566 kb on + strand, within MIT1002_00849 at 930.581 kb on - strand, within MIT1002_00849 at 930.637 kb on - strand, within MIT1002_00849 at 930.637 kb on - strand, within MIT1002_00849 at 930.667 kb on + strand, within MIT1002_00849 at 930.680 kb on + strand, within MIT1002_00849 at 930.719 kb on + strand, within MIT1002_00849 at 930.719 kb on + strand, within MIT1002_00849 at 930.727 kb on - strand, within MIT1002_00849 at 930.727 kb on - strand, within MIT1002_00849 at 930.727 kb on - strand, within MIT1002_00849 at 930.775 kb on - strand, within MIT1002_00849 at 930.829 kb on + strand, within MIT1002_00849 at 930.829 kb on + strand, within MIT1002_00849 at 930.837 kb on - strand, within MIT1002_00849 at 930.837 kb on - strand, within MIT1002_00849 at 930.837 kb on - strand, within MIT1002_00849 at 930.912 kb on - strand, within MIT1002_00849 at 930.951 kb on + strand, within MIT1002_00849 at 930.969 kb on - strand, within MIT1002_00849 at 930.978 kb on - strand, within MIT1002_00849 at 931.181 kb on + strand, within MIT1002_00849 at 931.255 kb on - strand, within MIT1002_00849 at 931.258 kb on + strand, within MIT1002_00849 at 931.258 kb on + strand, within MIT1002_00849 at 931.258 kb on + strand, within MIT1002_00849 at 931.266 kb on - strand at 931.266 kb on - strand at 931.271 kb on + strand at 931.285 kb on + strand at 931.285 kb on + strand at 931.285 kb on + strand at 931.285 kb on - strand at 931.285 kb on - strand at 931.287 kb on + strand at 931.287 kb on + strand at 931.293 kb on - strand at 931.293 kb on - strand at 931.295 kb on - strand at 931.389 kb on - strand at 931.407 kb on + strand at 931.415 kb on + strand at 931.415 kb on + strand at 931.415 kb on + strand at 931.423 kb on - strand at 931.423 kb on - strand at 931.423 kb on - strand at 931.491 kb on + strand at 931.499 kb on - strand at 931.499 kb on - strand at 931.499 kb on - strand at 931.548 kb on - strand at 931.603 kb on + strand at 931.624 kb on - strand at 931.685 kb on - strand at 931.685 kb on - strand at 931.685 kb on - strand at 931.693 kb on - strand at 931.704 kb on + strand at 931.712 kb on - strand at 931.712 kb on - strand at 931.714 kb on + strand at 931.714 kb on + strand at 931.722 kb on - strand at 931.722 kb on - strand at 931.722 kb on - strand at 931.722 kb on - strand at 931.767 kb on + strand at 931.776 kb on + strand at 932.023 kb on + strand, within MIT1002_00850 at 932.053 kb on + strand, within MIT1002_00850 at 932.089 kb on + strand, within MIT1002_00850 at 932.097 kb on - strand, within MIT1002_00850 at 932.221 kb on + strand, within MIT1002_00850 at 932.226 kb on - strand, within MIT1002_00850 at 932.230 kb on - strand, within MIT1002_00850 at 932.277 kb on + strand, within MIT1002_00850 at 932.329 kb on + strand, within MIT1002_00850 at 932.336 kb on - strand, within MIT1002_00850 at 932.343 kb on + strand, within MIT1002_00850 at 932.351 kb on - strand, within MIT1002_00850 at 932.366 kb on + strand, within MIT1002_00850 at 932.366 kb on - strand, within MIT1002_00850 at 932.383 kb on + strand, within MIT1002_00850 at 932.451 kb on + strand, within MIT1002_00850 at 932.469 kb on - strand, within MIT1002_00850 at 932.474 kb on + strand, within MIT1002_00850 at 932.474 kb on + strand, within MIT1002_00850 at 932.474 kb on + strand, within MIT1002_00850 at 932.482 kb on - strand, within MIT1002_00850 at 932.504 kb on + strand, within MIT1002_00850
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 4 remove 927,979 + +2.6 928,017 - -1.9 928,020 + -0.7 928,088 + MIT1002_00847 0.22 +1.0 928,184 + MIT1002_00847 0.57 +1.6 928,192 - MIT1002_00847 0.60 -0.8 928,192 - MIT1002_00847 0.60 -0.5 928,195 - MIT1002_00847 0.61 +0.5 928,312 + -0.8 928,326 - +0.3 928,419 + +0.9 928,443 - -0.0 928,451 - -0.8 928,451 - -2.1 928,453 - +0.1 928,569 - -0.7 928,626 - MIT1002_00848 0.26 +0.8 928,629 + MIT1002_00848 0.27 -1.2 928,637 - MIT1002_00848 0.29 +0.3 928,637 - MIT1002_00848 0.29 +0.1 928,645 - MIT1002_00848 0.31 -0.5 928,670 + MIT1002_00848 0.39 -1.3 928,729 + MIT1002_00848 0.57 -1.5 928,784 + MIT1002_00848 0.74 -0.5 928,784 + MIT1002_00848 0.74 -3.0 928,784 + MIT1002_00848 0.74 +0.9 928,792 - MIT1002_00848 0.76 -0.5 928,801 + MIT1002_00848 0.79 +0.2 928,803 + MIT1002_00848 0.80 -0.2 928,803 + MIT1002_00848 0.80 +1.5 928,804 - MIT1002_00848 0.80 -0.3 928,818 + MIT1002_00848 0.84 -3.2 928,846 + +0.1 928,858 - +0.9 928,859 - -2.0 928,860 - -1.1 929,060 - +0.1 929,072 - -0.7 929,083 - +0.7 929,093 - +0.2 929,111 + -1.1 929,201 + -0.0 929,209 - +0.5 929,209 - -0.9 929,270 + MIT1002_00849 0.12 -1.0 929,280 + MIT1002_00849 0.13 -0.8 929,293 - MIT1002_00849 0.13 +0.3 929,293 - MIT1002_00849 0.13 -0.3 929,305 + MIT1002_00849 0.14 +0.5 929,313 - MIT1002_00849 0.14 -0.5 929,345 + MIT1002_00849 0.15 +1.0 929,353 - MIT1002_00849 0.16 -0.2 929,366 + MIT1002_00849 0.16 +0.5 929,381 + MIT1002_00849 0.17 +0.9 929,381 + MIT1002_00849 0.17 -0.3 929,389 - MIT1002_00849 0.17 +0.4 929,389 - MIT1002_00849 0.17 +0.2 929,389 - MIT1002_00849 0.17 +0.6 929,411 - MIT1002_00849 0.18 +0.5 929,436 + MIT1002_00849 0.19 -0.2 929,444 + MIT1002_00849 0.19 -0.0 929,472 - MIT1002_00849 0.20 -0.4 929,482 - MIT1002_00849 0.21 -0.2 929,543 + MIT1002_00849 0.23 +1.4 929,582 - MIT1002_00849 0.24 -0.4 929,628 + MIT1002_00849 0.26 -0.6 929,636 - MIT1002_00849 0.27 +1.1 929,652 + MIT1002_00849 0.27 -0.1 929,660 - MIT1002_00849 0.27 -0.5 929,661 - MIT1002_00849 0.28 +0.2 929,719 + MIT1002_00849 0.30 -0.0 929,727 - MIT1002_00849 0.30 +0.3 929,776 + MIT1002_00849 0.32 -2.2 929,784 + MIT1002_00849 0.32 -0.6 929,833 - MIT1002_00849 0.34 -1.5 929,860 + MIT1002_00849 0.35 -1.1 929,925 + MIT1002_00849 0.38 -0.6 929,984 - MIT1002_00849 0.40 -2.2 930,004 + MIT1002_00849 0.41 -0.1 930,022 + MIT1002_00849 0.42 -2.0 930,136 + MIT1002_00849 0.46 -0.4 930,136 + MIT1002_00849 0.46 +0.9 930,160 - MIT1002_00849 0.47 +1.8 930,170 - MIT1002_00849 0.47 +0.3 930,172 + MIT1002_00849 0.47 +0.9 930,180 - MIT1002_00849 0.48 -0.6 930,180 - MIT1002_00849 0.48 -0.6 930,180 - MIT1002_00849 0.48 +1.7 930,204 - MIT1002_00849 0.49 -0.2 930,204 - MIT1002_00849 0.49 +1.1 930,290 + MIT1002_00849 0.52 +0.5 930,298 - MIT1002_00849 0.52 -0.3 930,298 - MIT1002_00849 0.52 +0.6 930,392 + MIT1002_00849 0.56 -0.6 930,394 + MIT1002_00849 0.56 +0.5 930,394 + MIT1002_00849 0.56 -1.3 930,394 + MIT1002_00849 0.56 +0.3 930,402 - MIT1002_00849 0.56 +0.0 930,402 - MIT1002_00849 0.56 -0.2 930,402 - MIT1002_00849 0.56 +1.2 930,402 - MIT1002_00849 0.56 +0.1 930,424 + MIT1002_00849 0.57 +0.1 930,432 - MIT1002_00849 0.58 +1.0 930,441 + MIT1002_00849 0.58 -1.5 930,456 - MIT1002_00849 0.58 +0.9 930,473 + MIT1002_00849 0.59 -0.9 930,503 - MIT1002_00849 0.60 +1.0 930,503 - MIT1002_00849 0.60 +0.8 930,525 + MIT1002_00849 0.61 -2.5 930,532 + MIT1002_00849 0.61 +1.1 930,533 - MIT1002_00849 0.61 +0.2 930,566 + MIT1002_00849 0.63 -0.1 930,581 - MIT1002_00849 0.63 -1.1 930,637 - MIT1002_00849 0.66 -1.0 930,637 - MIT1002_00849 0.66 -0.2 930,667 + MIT1002_00849 0.67 +0.9 930,680 + MIT1002_00849 0.67 -0.6 930,719 + MIT1002_00849 0.69 -0.6 930,719 + MIT1002_00849 0.69 +0.5 930,727 - MIT1002_00849 0.69 +0.7 930,727 - MIT1002_00849 0.69 +0.3 930,727 - MIT1002_00849 0.69 +1.3 930,775 - MIT1002_00849 0.71 -0.1 930,829 + MIT1002_00849 0.73 +0.8 930,829 + MIT1002_00849 0.73 -2.9 930,837 - MIT1002_00849 0.73 +0.8 930,837 - MIT1002_00849 0.73 -0.6 930,837 - MIT1002_00849 0.73 -1.4 930,912 - MIT1002_00849 0.76 -0.8 930,951 + MIT1002_00849 0.78 -0.9 930,969 - MIT1002_00849 0.78 -1.3 930,978 - MIT1002_00849 0.79 +0.3 931,181 + MIT1002_00849 0.87 +0.2 931,255 - MIT1002_00849 0.90 +0.2 931,258 + MIT1002_00849 0.90 -0.5 931,258 + MIT1002_00849 0.90 -0.5 931,258 + MIT1002_00849 0.90 -0.7 931,266 - +0.4 931,266 - -1.5 931,271 + +0.1 931,285 + -0.1 931,285 + -0.3 931,285 + -0.6 931,285 - +1.4 931,285 - -0.2 931,287 + +0.2 931,287 + +1.3 931,293 - +0.2 931,293 - -0.7 931,295 - -0.8 931,389 - -1.7 931,407 + -0.3 931,415 + -1.0 931,415 + -1.0 931,415 + +1.4 931,423 - +1.4 931,423 - -2.0 931,423 - +1.0 931,491 + +0.8 931,499 - +0.1 931,499 - +0.8 931,499 - +0.1 931,548 - +1.8 931,603 + -0.1 931,624 - -0.5 931,685 - +0.2 931,685 - -0.2 931,685 - +0.0 931,693 - -3.3 931,704 + -1.4 931,712 - -2.1 931,712 - -1.3 931,714 + +0.4 931,714 + -0.7 931,722 - -0.0 931,722 - -0.2 931,722 - +0.5 931,722 - +0.3 931,767 + -0.5 931,776 + -0.7 932,023 + MIT1002_00850 0.23 -1.7 932,053 + MIT1002_00850 0.25 -0.0 932,089 + MIT1002_00850 0.28 -2.8 932,097 - MIT1002_00850 0.28 +1.1 932,221 + MIT1002_00850 0.37 -0.1 932,226 - MIT1002_00850 0.37 +0.3 932,230 - MIT1002_00850 0.37 -1.5 932,277 + MIT1002_00850 0.41 -0.1 932,329 + MIT1002_00850 0.44 +3.6 932,336 - MIT1002_00850 0.45 -1.5 932,343 + MIT1002_00850 0.45 +1.3 932,351 - MIT1002_00850 0.46 +0.1 932,366 + MIT1002_00850 0.47 +1.3 932,366 - MIT1002_00850 0.47 -0.0 932,383 + MIT1002_00850 0.48 +2.5 932,451 + MIT1002_00850 0.52 +1.0 932,469 - MIT1002_00850 0.54 -0.7 932,474 + MIT1002_00850 0.54 -0.8 932,474 + MIT1002_00850 0.54 +0.6 932,474 + MIT1002_00850 0.54 -0.5 932,482 - MIT1002_00850 0.55 -1.3 932,504 + MIT1002_00850 0.56 -0.7
Or see this region's nucleotide sequence