Experiment: monoculture; Experiment C, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00013 and MIT1002_00014 are separated by 416 nucleotides MIT1002_00014 and MIT1002_00015 are separated by 421 nucleotides
MIT1002_00013: MIT1002_00013 - putative GTP-binding protein EngB, at 13,835 to 14,470
_00013
MIT1002_00014: MIT1002_00014 - DNA polymerase I, at 14,887 to 17,679
_00014
MIT1002_00015: MIT1002_00015 - Sigma cross-reacting protein 27A, at 18,101 to 18,754
_00015
Position (kb) 14
15
16
17
18 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 14.439 kb on + strand at 14.460 kb on + strand at 14.468 kb on - strand at 14.468 kb on - strand at 14.482 kb on - strand at 14.482 kb on - strand at 14.482 kb on - strand at 14.497 kb on + strand at 14.497 kb on + strand at 14.497 kb on + strand at 14.497 kb on + strand at 14.497 kb on + strand at 14.497 kb on + strand at 14.497 kb on + strand at 14.505 kb on - strand at 14.505 kb on - strand at 14.505 kb on - strand at 14.505 kb on - strand at 14.505 kb on - strand at 14.512 kb on + strand at 14.519 kb on + strand at 14.621 kb on - strand at 14.731 kb on + strand at 14.746 kb on - strand at 14.759 kb on + strand at 14.767 kb on + strand at 14.767 kb on - strand at 15.135 kb on - strand at 15.246 kb on - strand, within MIT1002_00014 at 15.247 kb on - strand, within MIT1002_00014 at 15.293 kb on - strand, within MIT1002_00014 at 15.369 kb on - strand, within MIT1002_00014 at 15.428 kb on - strand, within MIT1002_00014 at 15.541 kb on - strand, within MIT1002_00014 at 15.783 kb on - strand, within MIT1002_00014 at 15.848 kb on + strand, within MIT1002_00014 at 15.922 kb on - strand, within MIT1002_00014 at 15.969 kb on - strand, within MIT1002_00014 at 16.010 kb on - strand, within MIT1002_00014 at 16.031 kb on - strand, within MIT1002_00014 at 16.031 kb on - strand, within MIT1002_00014 at 16.083 kb on - strand, within MIT1002_00014 at 16.083 kb on - strand, within MIT1002_00014 at 16.083 kb on - strand, within MIT1002_00014 at 16.317 kb on + strand, within MIT1002_00014 at 16.317 kb on + strand, within MIT1002_00014 at 16.317 kb on + strand, within MIT1002_00014 at 16.320 kb on - strand, within MIT1002_00014 at 16.325 kb on - strand, within MIT1002_00014 at 16.325 kb on - strand, within MIT1002_00014 at 16.325 kb on - strand, within MIT1002_00014 at 16.325 kb on - strand, within MIT1002_00014 at 16.335 kb on - strand, within MIT1002_00014 at 16.335 kb on - strand, within MIT1002_00014 at 16.551 kb on - strand, within MIT1002_00014 at 16.731 kb on + strand, within MIT1002_00014 at 16.739 kb on - strand, within MIT1002_00014 at 17.728 kb on - strand at 17.728 kb on - strand at 17.733 kb on - strand at 17.733 kb on - strand at 17.733 kb on - strand at 17.733 kb on - strand at 17.733 kb on - strand at 17.755 kb on + strand at 17.756 kb on + strand at 17.764 kb on - strand at 17.990 kb on + strand at 18.003 kb on - strand at 18.007 kb on + strand at 18.129 kb on + strand at 18.129 kb on + strand at 18.152 kb on + strand at 18.160 kb on - strand at 18.160 kb on - strand at 18.160 kb on - strand at 18.160 kb on - strand at 18.219 kb on - strand, within MIT1002_00015 at 18.273 kb on - strand, within MIT1002_00015 at 18.309 kb on + strand, within MIT1002_00015 at 18.314 kb on + strand, within MIT1002_00015 at 18.322 kb on - strand, within MIT1002_00015 at 18.351 kb on - strand, within MIT1002_00015 at 18.403 kb on - strand, within MIT1002_00015 at 18.451 kb on + strand, within MIT1002_00015 at 18.471 kb on - strand, within MIT1002_00015 at 18.595 kb on - strand, within MIT1002_00015 at 18.606 kb on - strand, within MIT1002_00015 at 18.609 kb on + strand, within MIT1002_00015 at 18.611 kb on + strand, within MIT1002_00015 at 18.611 kb on + strand, within MIT1002_00015 at 18.616 kb on + strand, within MIT1002_00015 at 18.619 kb on - strand, within MIT1002_00015 at 18.619 kb on - strand, within MIT1002_00015 at 18.626 kb on + strand, within MIT1002_00015 at 18.666 kb on - strand, within MIT1002_00015
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 4 remove 14,439 + -0.6 14,460 + +0.6 14,468 - -0.9 14,468 - +0.9 14,482 - +0.9 14,482 - +0.9 14,482 - -0.3 14,497 + -0.1 14,497 + -0.2 14,497 + +0.5 14,497 + -0.7 14,497 + -0.4 14,497 + -1.9 14,497 + +0.4 14,505 - -2.8 14,505 - -1.3 14,505 - +1.7 14,505 - -1.6 14,505 - -1.8 14,512 + -1.3 14,519 + -1.9 14,621 - +0.8 14,731 + +1.3 14,746 - +0.8 14,759 + +0.8 14,767 + +0.5 14,767 - +1.8 15,135 - +0.3 15,246 - MIT1002_00014 0.13 +0.0 15,247 - MIT1002_00014 0.13 -0.4 15,293 - MIT1002_00014 0.15 -1.0 15,369 - MIT1002_00014 0.17 -0.4 15,428 - MIT1002_00014 0.19 -1.6 15,541 - MIT1002_00014 0.23 -0.9 15,783 - MIT1002_00014 0.32 -1.0 15,848 + MIT1002_00014 0.34 -0.2 15,922 - MIT1002_00014 0.37 -0.9 15,969 - MIT1002_00014 0.39 -0.9 16,010 - MIT1002_00014 0.40 -1.9 16,031 - MIT1002_00014 0.41 -1.7 16,031 - MIT1002_00014 0.41 +0.6 16,083 - MIT1002_00014 0.43 -1.9 16,083 - MIT1002_00014 0.43 -2.7 16,083 - MIT1002_00014 0.43 -1.2 16,317 + MIT1002_00014 0.51 +0.6 16,317 + MIT1002_00014 0.51 -1.0 16,317 + MIT1002_00014 0.51 -0.4 16,320 - MIT1002_00014 0.51 -2.8 16,325 - MIT1002_00014 0.51 -0.2 16,325 - MIT1002_00014 0.51 -2.0 16,325 - MIT1002_00014 0.51 -1.1 16,325 - MIT1002_00014 0.51 -2.4 16,335 - MIT1002_00014 0.52 -0.2 16,335 - MIT1002_00014 0.52 -0.4 16,551 - MIT1002_00014 0.60 -2.3 16,731 + MIT1002_00014 0.66 -1.7 16,739 - MIT1002_00014 0.66 -1.9 17,728 - -0.7 17,728 - +1.8 17,733 - -0.1 17,733 - -1.1 17,733 - +0.1 17,733 - -0.7 17,733 - +0.9 17,755 + -1.4 17,756 + -2.1 17,764 - -0.1 17,990 + +0.8 18,003 - -0.3 18,007 + +0.5 18,129 + -0.2 18,129 + -0.2 18,152 + -0.2 18,160 - +1.0 18,160 - +0.9 18,160 - -2.4 18,160 - -0.8 18,219 - MIT1002_00015 0.18 -0.9 18,273 - MIT1002_00015 0.26 +0.3 18,309 + MIT1002_00015 0.32 +0.6 18,314 + MIT1002_00015 0.33 +0.3 18,322 - MIT1002_00015 0.34 -2.2 18,351 - MIT1002_00015 0.38 -1.4 18,403 - MIT1002_00015 0.46 -0.4 18,451 + MIT1002_00015 0.54 -1.0 18,471 - MIT1002_00015 0.57 -1.3 18,595 - MIT1002_00015 0.76 +0.0 18,606 - MIT1002_00015 0.77 +0.4 18,609 + MIT1002_00015 0.78 -0.2 18,611 + MIT1002_00015 0.78 -2.0 18,611 + MIT1002_00015 0.78 +1.1 18,616 + MIT1002_00015 0.79 -1.1 18,619 - MIT1002_00015 0.79 -0.4 18,619 - MIT1002_00015 0.79 -0.9 18,626 + MIT1002_00015 0.80 -0.1 18,666 - MIT1002_00015 0.86 -0.5
Or see this region's nucleotide sequence