Experiment: monoculture; Experiment C, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02386 and MIT1002_02387 are separated by 30 nucleotides MIT1002_02387 and MIT1002_02388 are separated by 83 nucleotides MIT1002_02388 and MIT1002_02389 are separated by 12 nucleotides MIT1002_02389 and MIT1002_02390 overlap by 11 nucleotides MIT1002_02390 and MIT1002_02391 are separated by 0 nucleotides MIT1002_02391 and MIT1002_02392 are separated by 265 nucleotides
MIT1002_02386: MIT1002_02386 - Small integral membrane protein, at 2,662,510 to 2,662,686
_02386
MIT1002_02387: MIT1002_02387 - hypothetical protein, at 2,662,717 to 2,662,911
_02387
MIT1002_02388: MIT1002_02388 - hypothetical protein, at 2,662,995 to 2,663,213
_02388
MIT1002_02389: MIT1002_02389 - hypothetical protein, at 2,663,226 to 2,666,168
_02389
MIT1002_02390: MIT1002_02390 - hypothetical protein, at 2,666,158 to 2,666,451
_02390
MIT1002_02391: MIT1002_02391 - putative membrane protein, at 2,666,452 to 2,666,778
_02391
MIT1002_02392: MIT1002_02392 - Amino-acid carrier protein AlsT, at 2,667,044 to 2,668,702
_02392
Position (kb)
2663
2664
2665
2666
2667 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2662.254 kb on - strand at 2662.262 kb on + strand at 2662.305 kb on + strand at 2662.319 kb on + strand at 2662.319 kb on + strand at 2662.494 kb on + strand at 2662.598 kb on - strand, within MIT1002_02386 at 2662.778 kb on - strand, within MIT1002_02387 at 2662.778 kb on - strand, within MIT1002_02387 at 2662.778 kb on - strand, within MIT1002_02387 at 2662.780 kb on - strand, within MIT1002_02387 at 2662.824 kb on - strand, within MIT1002_02387 at 2662.897 kb on + strand at 2662.931 kb on + strand at 2662.966 kb on + strand at 2663.019 kb on - strand, within MIT1002_02388 at 2663.037 kb on - strand, within MIT1002_02388 at 2663.039 kb on - strand, within MIT1002_02388 at 2663.063 kb on + strand, within MIT1002_02388 at 2663.063 kb on + strand, within MIT1002_02388 at 2663.063 kb on + strand, within MIT1002_02388 at 2663.071 kb on - strand, within MIT1002_02388 at 2663.071 kb on - strand, within MIT1002_02388 at 2663.089 kb on - strand, within MIT1002_02388 at 2663.120 kb on + strand, within MIT1002_02388 at 2663.144 kb on + strand, within MIT1002_02388 at 2663.152 kb on - strand, within MIT1002_02388 at 2663.195 kb on + strand at 2663.203 kb on - strand at 2663.283 kb on + strand at 2663.370 kb on - strand at 2663.384 kb on + strand at 2663.385 kb on - strand at 2663.389 kb on + strand at 2663.472 kb on + strand at 2663.575 kb on - strand, within MIT1002_02389 at 2663.580 kb on - strand, within MIT1002_02389 at 2663.829 kb on + strand, within MIT1002_02389 at 2663.829 kb on + strand, within MIT1002_02389 at 2663.837 kb on - strand, within MIT1002_02389 at 2663.837 kb on - strand, within MIT1002_02389 at 2663.881 kb on + strand, within MIT1002_02389 at 2663.881 kb on + strand, within MIT1002_02389 at 2663.889 kb on - strand, within MIT1002_02389 at 2663.889 kb on - strand, within MIT1002_02389 at 2663.917 kb on - strand, within MIT1002_02389 at 2664.003 kb on + strand, within MIT1002_02389 at 2664.016 kb on - strand, within MIT1002_02389 at 2664.142 kb on + strand, within MIT1002_02389 at 2664.145 kb on - strand, within MIT1002_02389 at 2664.145 kb on - strand, within MIT1002_02389 at 2664.155 kb on + strand, within MIT1002_02389 at 2664.155 kb on + strand, within MIT1002_02389 at 2664.155 kb on + strand, within MIT1002_02389 at 2664.163 kb on - strand, within MIT1002_02389 at 2664.214 kb on + strand, within MIT1002_02389 at 2664.222 kb on - strand, within MIT1002_02389 at 2664.307 kb on - strand, within MIT1002_02389 at 2664.411 kb on + strand, within MIT1002_02389 at 2664.419 kb on - strand, within MIT1002_02389 at 2664.419 kb on - strand, within MIT1002_02389 at 2664.441 kb on + strand, within MIT1002_02389 at 2664.454 kb on + strand, within MIT1002_02389 at 2664.586 kb on + strand, within MIT1002_02389 at 2664.677 kb on + strand, within MIT1002_02389 at 2664.753 kb on + strand, within MIT1002_02389 at 2664.786 kb on - strand, within MIT1002_02389 at 2664.794 kb on - strand, within MIT1002_02389 at 2664.927 kb on + strand, within MIT1002_02389 at 2664.939 kb on + strand, within MIT1002_02389 at 2664.947 kb on - strand, within MIT1002_02389 at 2664.957 kb on - strand, within MIT1002_02389 at 2665.085 kb on + strand, within MIT1002_02389 at 2665.093 kb on - strand, within MIT1002_02389 at 2665.104 kb on - strand, within MIT1002_02389 at 2665.154 kb on + strand, within MIT1002_02389 at 2665.154 kb on + strand, within MIT1002_02389 at 2665.162 kb on - strand, within MIT1002_02389 at 2665.174 kb on - strand, within MIT1002_02389 at 2665.226 kb on + strand, within MIT1002_02389 at 2665.233 kb on - strand, within MIT1002_02389 at 2665.256 kb on + strand, within MIT1002_02389 at 2665.277 kb on + strand, within MIT1002_02389 at 2665.330 kb on - strand, within MIT1002_02389 at 2665.407 kb on + strand, within MIT1002_02389 at 2665.455 kb on + strand, within MIT1002_02389 at 2665.529 kb on + strand, within MIT1002_02389 at 2665.529 kb on + strand, within MIT1002_02389 at 2665.529 kb on + strand, within MIT1002_02389 at 2665.529 kb on + strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.537 kb on - strand, within MIT1002_02389 at 2665.585 kb on + strand, within MIT1002_02389 at 2665.631 kb on - strand, within MIT1002_02389 at 2665.661 kb on + strand, within MIT1002_02389 at 2665.662 kb on + strand, within MIT1002_02389 at 2665.740 kb on - strand, within MIT1002_02389 at 2665.835 kb on + strand, within MIT1002_02389 at 2665.879 kb on - strand at 2665.894 kb on - strand at 2665.909 kb on - strand at 2665.985 kb on + strand at 2665.993 kb on - strand at 2666.022 kb on - strand at 2666.077 kb on + strand at 2666.186 kb on + strand at 2666.194 kb on - strand, within MIT1002_02390 at 2666.242 kb on - strand, within MIT1002_02390 at 2666.245 kb on + strand, within MIT1002_02390 at 2666.281 kb on - strand, within MIT1002_02390 at 2666.330 kb on + strand, within MIT1002_02390 at 2666.358 kb on - strand, within MIT1002_02390 at 2666.406 kb on + strand, within MIT1002_02390 at 2666.428 kb on + strand at 2666.436 kb on - strand at 2666.436 kb on - strand at 2666.487 kb on + strand, within MIT1002_02391 at 2666.774 kb on - strand at 2666.779 kb on + strand at 2666.908 kb on - strand at 2666.936 kb on + strand at 2666.999 kb on - strand at 2667.009 kb on + strand at 2667.019 kb on + strand at 2667.019 kb on + strand at 2667.068 kb on + strand at 2667.111 kb on - strand at 2667.164 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 3 remove 2,662,254 - -1.4 2,662,262 + +1.5 2,662,305 + -2.1 2,662,319 + -0.5 2,662,319 + -0.5 2,662,494 + -0.1 2,662,598 - MIT1002_02386 0.50 -0.7 2,662,778 - MIT1002_02387 0.31 +0.7 2,662,778 - MIT1002_02387 0.31 -0.6 2,662,778 - MIT1002_02387 0.31 +0.1 2,662,780 - MIT1002_02387 0.32 -1.5 2,662,824 - MIT1002_02387 0.55 +0.1 2,662,897 + -0.4 2,662,931 + +0.2 2,662,966 + +0.4 2,663,019 - MIT1002_02388 0.11 +0.9 2,663,037 - MIT1002_02388 0.19 -0.2 2,663,039 - MIT1002_02388 0.20 -0.5 2,663,063 + MIT1002_02388 0.31 -1.6 2,663,063 + MIT1002_02388 0.31 +1.9 2,663,063 + MIT1002_02388 0.31 -0.2 2,663,071 - MIT1002_02388 0.35 -0.1 2,663,071 - MIT1002_02388 0.35 -2.0 2,663,089 - MIT1002_02388 0.43 +0.7 2,663,120 + MIT1002_02388 0.57 -0.4 2,663,144 + MIT1002_02388 0.68 +0.5 2,663,152 - MIT1002_02388 0.72 -1.3 2,663,195 + -0.4 2,663,203 - -1.4 2,663,283 + -0.5 2,663,370 - -1.3 2,663,384 + +1.0 2,663,385 - -0.1 2,663,389 + -1.9 2,663,472 + +0.2 2,663,575 - MIT1002_02389 0.12 +0.2 2,663,580 - MIT1002_02389 0.12 -1.0 2,663,829 + MIT1002_02389 0.20 -0.3 2,663,829 + MIT1002_02389 0.20 +0.5 2,663,837 - MIT1002_02389 0.21 +0.1 2,663,837 - MIT1002_02389 0.21 +0.4 2,663,881 + MIT1002_02389 0.22 -0.7 2,663,881 + MIT1002_02389 0.22 -1.8 2,663,889 - MIT1002_02389 0.23 -2.7 2,663,889 - MIT1002_02389 0.23 -0.8 2,663,917 - MIT1002_02389 0.23 -0.5 2,664,003 + MIT1002_02389 0.26 -1.0 2,664,016 - MIT1002_02389 0.27 -0.3 2,664,142 + MIT1002_02389 0.31 +0.3 2,664,145 - MIT1002_02389 0.31 +1.9 2,664,145 - MIT1002_02389 0.31 -0.3 2,664,155 + MIT1002_02389 0.32 +0.4 2,664,155 + MIT1002_02389 0.32 -0.3 2,664,155 + MIT1002_02389 0.32 +1.4 2,664,163 - MIT1002_02389 0.32 -0.9 2,664,214 + MIT1002_02389 0.34 +1.3 2,664,222 - MIT1002_02389 0.34 -1.3 2,664,307 - MIT1002_02389 0.37 -0.5 2,664,411 + MIT1002_02389 0.40 +1.5 2,664,419 - MIT1002_02389 0.41 -0.8 2,664,419 - MIT1002_02389 0.41 -0.1 2,664,441 + MIT1002_02389 0.41 -1.4 2,664,454 + MIT1002_02389 0.42 -0.1 2,664,586 + MIT1002_02389 0.46 -0.9 2,664,677 + MIT1002_02389 0.49 -1.2 2,664,753 + MIT1002_02389 0.52 -0.5 2,664,786 - MIT1002_02389 0.53 -1.3 2,664,794 - MIT1002_02389 0.53 -0.7 2,664,927 + MIT1002_02389 0.58 +0.9 2,664,939 + MIT1002_02389 0.58 -0.0 2,664,947 - MIT1002_02389 0.58 +0.6 2,664,957 - MIT1002_02389 0.59 -2.3 2,665,085 + MIT1002_02389 0.63 +0.1 2,665,093 - MIT1002_02389 0.63 +1.1 2,665,104 - MIT1002_02389 0.64 -0.2 2,665,154 + MIT1002_02389 0.66 -0.9 2,665,154 + MIT1002_02389 0.66 -0.8 2,665,162 - MIT1002_02389 0.66 -1.1 2,665,174 - MIT1002_02389 0.66 -0.2 2,665,226 + MIT1002_02389 0.68 -0.8 2,665,233 - MIT1002_02389 0.68 +1.0 2,665,256 + MIT1002_02389 0.69 -0.0 2,665,277 + MIT1002_02389 0.70 +0.2 2,665,330 - MIT1002_02389 0.71 -0.9 2,665,407 + MIT1002_02389 0.74 -0.0 2,665,455 + MIT1002_02389 0.76 -0.6 2,665,529 + MIT1002_02389 0.78 +3.5 2,665,529 + MIT1002_02389 0.78 -1.1 2,665,529 + MIT1002_02389 0.78 -2.8 2,665,529 + MIT1002_02389 0.78 +0.1 2,665,537 - MIT1002_02389 0.79 -1.5 2,665,537 - MIT1002_02389 0.79 -0.0 2,665,537 - MIT1002_02389 0.79 -1.0 2,665,537 - MIT1002_02389 0.79 -0.6 2,665,537 - MIT1002_02389 0.79 -0.0 2,665,537 - MIT1002_02389 0.79 +1.5 2,665,537 - MIT1002_02389 0.79 +0.4 2,665,537 - MIT1002_02389 0.79 -2.0 2,665,585 + MIT1002_02389 0.80 -0.9 2,665,631 - MIT1002_02389 0.82 -1.2 2,665,661 + MIT1002_02389 0.83 +0.4 2,665,662 + MIT1002_02389 0.83 -0.1 2,665,740 - MIT1002_02389 0.85 -0.9 2,665,835 + MIT1002_02389 0.89 -2.4 2,665,879 - +0.5 2,665,894 - +1.9 2,665,909 - -0.2 2,665,985 + +0.2 2,665,993 - -1.0 2,666,022 - +1.4 2,666,077 + -1.1 2,666,186 + -2.8 2,666,194 - MIT1002_02390 0.12 +1.5 2,666,242 - MIT1002_02390 0.29 -1.4 2,666,245 + MIT1002_02390 0.30 -0.1 2,666,281 - MIT1002_02390 0.42 -0.5 2,666,330 + MIT1002_02390 0.59 -0.6 2,666,358 - MIT1002_02390 0.68 -1.6 2,666,406 + MIT1002_02390 0.84 -3.4 2,666,428 + -0.7 2,666,436 - -1.5 2,666,436 - -2.1 2,666,487 + MIT1002_02391 0.11 -0.8 2,666,774 - -0.8 2,666,779 + +1.0 2,666,908 - +0.6 2,666,936 + -0.3 2,666,999 - -0.3 2,667,009 + +1.1 2,667,019 + -1.0 2,667,019 + -0.3 2,667,068 + -2.4 2,667,111 - +0.9 2,667,164 + -2.5
Or see this region's nucleotide sequence