Experiment: monoculture; Experiment C, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02167 and MIT1002_02168 are separated by 56 nucleotides MIT1002_02168 and MIT1002_02169 overlap by 1 nucleotides MIT1002_02169 and MIT1002_02170 overlap by 1 nucleotides MIT1002_02170 and MIT1002_02171 overlap by 4 nucleotides MIT1002_02171 and MIT1002_02172 are separated by 270 nucleotides
MIT1002_02167: MIT1002_02167 - Modulator of FtsH protease YccA, at 2,423,006 to 2,423,671
_02167
MIT1002_02168: MIT1002_02168 - Sulfurtransferase TusD, at 2,423,728 to 2,424,117
_02168
MIT1002_02169: MIT1002_02169 - tRNA 2-thiouridine synthesizing protein C, at 2,424,117 to 2,424,485
_02169
MIT1002_02170: MIT1002_02170 - hypothetical protein, at 2,424,485 to 2,424,805
_02170
MIT1002_02171: MIT1002_02171 - Sulfurtransferase TusE, at 2,424,802 to 2,425,149
_02171
MIT1002_02172: MIT1002_02172 - Protein required for attachment to host cells, at 2,425,420 to 2,425,857
_02172
Position (kb)
2424
2425 Strain fitness (log2 ratio)
-1
0
1
2 at 2423.126 kb on + strand, within MIT1002_02167 at 2423.318 kb on + strand, within MIT1002_02167 at 2423.334 kb on + strand, within MIT1002_02167 at 2423.342 kb on + strand, within MIT1002_02167 at 2423.435 kb on + strand, within MIT1002_02167 at 2423.490 kb on + strand, within MIT1002_02167 at 2423.497 kb on + strand, within MIT1002_02167 at 2425.174 kb on + strand at 2425.249 kb on - strand at 2425.270 kb on - strand at 2425.289 kb on - strand at 2425.384 kb on + strand at 2425.418 kb on + strand at 2425.424 kb on - strand at 2425.424 kb on - strand at 2425.424 kb on - strand at 2425.464 kb on + strand, within MIT1002_02172 at 2425.471 kb on + strand, within MIT1002_02172 at 2425.471 kb on + strand, within MIT1002_02172 at 2425.479 kb on - strand, within MIT1002_02172 at 2425.479 kb on - strand, within MIT1002_02172
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 3 remove 2,423,126 + MIT1002_02167 0.18 +0.6 2,423,318 + MIT1002_02167 0.47 +1.8 2,423,334 + MIT1002_02167 0.49 -0.4 2,423,342 + MIT1002_02167 0.50 -0.2 2,423,435 + MIT1002_02167 0.64 +0.3 2,423,490 + MIT1002_02167 0.73 +0.2 2,423,497 + MIT1002_02167 0.74 +0.8 2,425,174 + -0.7 2,425,249 - +0.8 2,425,270 - +1.3 2,425,289 - -0.8 2,425,384 + +0.5 2,425,418 + +0.1 2,425,424 - +0.5 2,425,424 - +0.1 2,425,424 - +0.3 2,425,464 + MIT1002_02172 0.10 -0.7 2,425,471 + MIT1002_02172 0.12 +0.2 2,425,471 + MIT1002_02172 0.12 +0.8 2,425,479 - MIT1002_02172 0.13 +0.5 2,425,479 - MIT1002_02172 0.13 +0.1
Or see this region's nucleotide sequence