Experiment: monoculture; Experiment C, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01193 and MIT1002_01194 are separated by 288 nucleotides MIT1002_01194 and MIT1002_01195 are separated by 219 nucleotides
MIT1002_01193: MIT1002_01193 - Arginine--tRNA ligase, at 1,307,737 to 1,309,467
_01193
MIT1002_01194: MIT1002_01194 - FMN-dependent NADPH-azoreductase, at 1,309,756 to 1,310,322
_01194
MIT1002_01195: MIT1002_01195 - type IV pilus biogenesis/stability protein PilW, at 1,310,542 to 1,312,500
_01195
Position (kb)
1309
1310
1311 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9 at 1309.476 kb on + strand at 1309.477 kb on - strand at 1309.503 kb on + strand at 1309.590 kb on - strand at 1309.605 kb on + strand at 1309.661 kb on - strand at 1309.665 kb on - strand at 1309.692 kb on + strand at 1309.701 kb on - strand at 1309.701 kb on - strand at 1309.728 kb on - strand at 1309.825 kb on + strand, within MIT1002_01194 at 1309.850 kb on + strand, within MIT1002_01194 at 1309.857 kb on + strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.858 kb on - strand, within MIT1002_01194 at 1309.873 kb on - strand, within MIT1002_01194 at 1309.937 kb on + strand, within MIT1002_01194 at 1309.947 kb on - strand, within MIT1002_01194 at 1310.004 kb on - strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.012 kb on + strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.020 kb on - strand, within MIT1002_01194 at 1310.162 kb on - strand, within MIT1002_01194 at 1310.183 kb on + strand, within MIT1002_01194 at 1310.191 kb on - strand, within MIT1002_01194 at 1310.191 kb on - strand, within MIT1002_01194 at 1310.217 kb on + strand, within MIT1002_01194 at 1310.269 kb on - strand at 1310.269 kb on - strand at 1310.386 kb on + strand at 1310.386 kb on + strand at 1310.394 kb on - strand at 1310.394 kb on - strand at 1310.395 kb on + strand at 1310.401 kb on + strand at 1310.446 kb on - strand at 1310.477 kb on + strand at 1310.575 kb on + strand at 1310.596 kb on + strand at 1310.630 kb on + strand at 1310.666 kb on - strand at 1310.801 kb on - strand, within MIT1002_01195 at 1310.801 kb on - strand, within MIT1002_01195 at 1310.838 kb on - strand, within MIT1002_01195 at 1310.968 kb on - strand, within MIT1002_01195 at 1310.978 kb on + strand, within MIT1002_01195 at 1311.044 kb on + strand, within MIT1002_01195 at 1311.117 kb on + strand, within MIT1002_01195 at 1311.125 kb on - strand, within MIT1002_01195 at 1311.189 kb on - strand, within MIT1002_01195 at 1311.189 kb on - strand, within MIT1002_01195 at 1311.274 kb on + strand, within MIT1002_01195 at 1311.275 kb on + strand, within MIT1002_01195 at 1311.283 kb on - strand, within MIT1002_01195 at 1311.317 kb on + strand, within MIT1002_01195
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 3 remove 1,309,476 + +1.0 1,309,477 - +1.4 1,309,503 + +0.0 1,309,590 - -1.0 1,309,605 + -0.2 1,309,661 - -0.1 1,309,665 - -0.2 1,309,692 + -0.9 1,309,701 - +1.1 1,309,701 - -1.0 1,309,728 - +0.8 1,309,825 + MIT1002_01194 0.12 +0.4 1,309,850 + MIT1002_01194 0.17 -1.2 1,309,857 + MIT1002_01194 0.18 -0.1 1,309,858 - MIT1002_01194 0.18 +0.8 1,309,858 - MIT1002_01194 0.18 -0.2 1,309,858 - MIT1002_01194 0.18 -0.1 1,309,858 - MIT1002_01194 0.18 -2.6 1,309,873 - MIT1002_01194 0.21 -0.6 1,309,937 + MIT1002_01194 0.32 +0.5 1,309,947 - MIT1002_01194 0.34 -0.6 1,310,004 - MIT1002_01194 0.44 +0.4 1,310,012 + MIT1002_01194 0.45 +0.1 1,310,012 + MIT1002_01194 0.45 +2.5 1,310,012 + MIT1002_01194 0.45 -2.4 1,310,012 + MIT1002_01194 0.45 +0.3 1,310,012 + MIT1002_01194 0.45 +0.6 1,310,020 - MIT1002_01194 0.47 +0.1 1,310,020 - MIT1002_01194 0.47 -1.2 1,310,020 - MIT1002_01194 0.47 +9.4 1,310,020 - MIT1002_01194 0.47 +0.1 1,310,162 - MIT1002_01194 0.72 +1.5 1,310,183 + MIT1002_01194 0.75 -2.1 1,310,191 - MIT1002_01194 0.77 -1.5 1,310,191 - MIT1002_01194 0.77 +0.7 1,310,217 + MIT1002_01194 0.81 +2.5 1,310,269 - -2.3 1,310,269 - -2.1 1,310,386 + +0.1 1,310,386 + -0.2 1,310,394 - +0.8 1,310,394 - +0.6 1,310,395 + +0.6 1,310,401 + +0.8 1,310,446 - +0.3 1,310,477 + +0.5 1,310,575 + +0.5 1,310,596 + -0.1 1,310,630 + +0.2 1,310,666 - +0.3 1,310,801 - MIT1002_01195 0.13 +0.4 1,310,801 - MIT1002_01195 0.13 -1.3 1,310,838 - MIT1002_01195 0.15 -0.6 1,310,968 - MIT1002_01195 0.22 -0.2 1,310,978 + MIT1002_01195 0.22 +0.3 1,311,044 + MIT1002_01195 0.26 -1.5 1,311,117 + MIT1002_01195 0.29 -0.2 1,311,125 - MIT1002_01195 0.30 +0.6 1,311,189 - MIT1002_01195 0.33 +0.3 1,311,189 - MIT1002_01195 0.33 -0.4 1,311,274 + MIT1002_01195 0.37 +5.0 1,311,275 + MIT1002_01195 0.37 -2.0 1,311,283 - MIT1002_01195 0.38 +0.7 1,311,317 + MIT1002_01195 0.40 -0.6
Or see this region's nucleotide sequence