Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02507 and MIT1002_02508 are separated by 94 nucleotides MIT1002_02508 and MIT1002_02509 are separated by 197 nucleotides MIT1002_02509 and MIT1002_02510 are separated by 247 nucleotides MIT1002_02510 and MIT1002_02511 are separated by 449 nucleotides
MIT1002_02507: MIT1002_02507 - Sucrose phosphorylase, at 2,795,928 to 2,797,424
_02507
MIT1002_02508: MIT1002_02508 - putative HTH-type transcriptional regulator YtcD, at 2,797,519 to 2,797,941
_02508
MIT1002_02509: MIT1002_02509 - putative acrylyl-CoA reductase AcuI, at 2,798,139 to 2,799,128
_02509
MIT1002_02510: MIT1002_02510 - AhpC/TSA family protein, at 2,799,376 to 2,799,675
_02510
MIT1002_02511: MIT1002_02511 - hypothetical protein, at 2,800,125 to 2,800,553
_02511
Position (kb)
2798
2799
2800 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2797.205 kb on + strand, within MIT1002_02507 at 2797.212 kb on + strand, within MIT1002_02507 at 2797.213 kb on - strand, within MIT1002_02507 at 2797.213 kb on - strand, within MIT1002_02507 at 2797.260 kb on + strand, within MIT1002_02507 at 2797.332 kb on - strand at 2797.364 kb on - strand at 2797.370 kb on - strand at 2797.375 kb on - strand at 2797.393 kb on + strand at 2797.424 kb on + strand at 2797.469 kb on - strand at 2797.521 kb on + strand at 2797.541 kb on + strand at 2797.543 kb on - strand at 2797.571 kb on - strand, within MIT1002_02508 at 2797.571 kb on - strand, within MIT1002_02508 at 2797.575 kb on + strand, within MIT1002_02508 at 2797.583 kb on - strand, within MIT1002_02508 at 2797.583 kb on - strand, within MIT1002_02508 at 2797.605 kb on - strand, within MIT1002_02508 at 2797.626 kb on - strand, within MIT1002_02508 at 2797.646 kb on - strand, within MIT1002_02508 at 2797.648 kb on + strand, within MIT1002_02508 at 2797.714 kb on - strand, within MIT1002_02508 at 2797.715 kb on + strand, within MIT1002_02508 at 2797.814 kb on - strand, within MIT1002_02508 at 2797.822 kb on + strand, within MIT1002_02508 at 2797.843 kb on + strand, within MIT1002_02508 at 2797.860 kb on + strand, within MIT1002_02508 at 2797.913 kb on + strand at 2797.916 kb on - strand at 2797.949 kb on + strand at 2798.102 kb on + strand at 2798.136 kb on + strand at 2798.147 kb on + strand at 2798.154 kb on + strand at 2798.155 kb on - strand at 2798.155 kb on - strand at 2798.219 kb on + strand at 2798.250 kb on + strand, within MIT1002_02509 at 2798.251 kb on - strand, within MIT1002_02509 at 2798.294 kb on + strand, within MIT1002_02509 at 2798.294 kb on + strand, within MIT1002_02509 at 2798.353 kb on + strand, within MIT1002_02509 at 2798.361 kb on - strand, within MIT1002_02509 at 2798.361 kb on - strand, within MIT1002_02509 at 2798.361 kb on - strand, within MIT1002_02509 at 2798.363 kb on - strand, within MIT1002_02509 at 2798.418 kb on - strand, within MIT1002_02509 at 2798.439 kb on + strand, within MIT1002_02509 at 2798.439 kb on - strand, within MIT1002_02509 at 2798.509 kb on + strand, within MIT1002_02509 at 2798.527 kb on - strand, within MIT1002_02509 at 2798.552 kb on - strand, within MIT1002_02509 at 2798.553 kb on + strand, within MIT1002_02509 at 2798.622 kb on + strand, within MIT1002_02509 at 2798.678 kb on - strand, within MIT1002_02509 at 2798.704 kb on - strand, within MIT1002_02509 at 2798.741 kb on - strand, within MIT1002_02509 at 2798.842 kb on + strand, within MIT1002_02509 at 2798.860 kb on - strand, within MIT1002_02509 at 2798.917 kb on + strand, within MIT1002_02509 at 2798.965 kb on + strand, within MIT1002_02509 at 2798.965 kb on + strand, within MIT1002_02509 at 2799.107 kb on - strand at 2799.122 kb on + strand at 2799.130 kb on - strand at 2799.130 kb on - strand at 2799.222 kb on + strand at 2799.260 kb on - strand at 2799.528 kb on - strand, within MIT1002_02510 at 2799.537 kb on + strand, within MIT1002_02510 at 2799.704 kb on + strand at 2799.712 kb on - strand at 2799.716 kb on + strand at 2799.726 kb on + strand at 2799.772 kb on - strand at 2799.899 kb on - strand at 2800.001 kb on - strand at 2800.020 kb on + strand at 2800.028 kb on - strand at 2800.115 kb on + strand at 2800.118 kb on - strand at 2800.120 kb on + strand at 2800.128 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 2,797,205 + MIT1002_02507 0.85 +0.6 2,797,212 + MIT1002_02507 0.86 -0.4 2,797,213 - MIT1002_02507 0.86 -0.3 2,797,213 - MIT1002_02507 0.86 +0.8 2,797,260 + MIT1002_02507 0.89 +1.8 2,797,332 - -0.5 2,797,364 - +0.5 2,797,370 - -2.2 2,797,375 - +0.7 2,797,393 + +0.4 2,797,424 + +1.0 2,797,469 - -0.1 2,797,521 + +0.7 2,797,541 + -0.0 2,797,543 - -1.9 2,797,571 - MIT1002_02508 0.12 -0.5 2,797,571 - MIT1002_02508 0.12 -0.0 2,797,575 + MIT1002_02508 0.13 +1.1 2,797,583 - MIT1002_02508 0.15 +1.1 2,797,583 - MIT1002_02508 0.15 +0.6 2,797,605 - MIT1002_02508 0.20 -0.9 2,797,626 - MIT1002_02508 0.25 -1.6 2,797,646 - MIT1002_02508 0.30 +1.1 2,797,648 + MIT1002_02508 0.30 +0.6 2,797,714 - MIT1002_02508 0.46 -0.8 2,797,715 + MIT1002_02508 0.46 -0.3 2,797,814 - MIT1002_02508 0.70 -0.4 2,797,822 + MIT1002_02508 0.72 -1.1 2,797,843 + MIT1002_02508 0.77 +0.1 2,797,860 + MIT1002_02508 0.81 +0.7 2,797,913 + -0.3 2,797,916 - -1.0 2,797,949 + -0.4 2,798,102 + +0.4 2,798,136 + +0.2 2,798,147 + -0.8 2,798,154 + +0.4 2,798,155 - -0.4 2,798,155 - +0.7 2,798,219 + +0.3 2,798,250 + MIT1002_02509 0.11 +0.8 2,798,251 - MIT1002_02509 0.11 -0.3 2,798,294 + MIT1002_02509 0.16 -2.1 2,798,294 + MIT1002_02509 0.16 +0.5 2,798,353 + MIT1002_02509 0.22 -0.8 2,798,361 - MIT1002_02509 0.22 -0.0 2,798,361 - MIT1002_02509 0.22 +1.2 2,798,361 - MIT1002_02509 0.22 +1.4 2,798,363 - MIT1002_02509 0.23 -0.1 2,798,418 - MIT1002_02509 0.28 +0.0 2,798,439 + MIT1002_02509 0.30 +1.8 2,798,439 - MIT1002_02509 0.30 +0.4 2,798,509 + MIT1002_02509 0.37 -1.1 2,798,527 - MIT1002_02509 0.39 -0.1 2,798,552 - MIT1002_02509 0.42 -2.6 2,798,553 + MIT1002_02509 0.42 +2.3 2,798,622 + MIT1002_02509 0.49 -0.3 2,798,678 - MIT1002_02509 0.54 +0.7 2,798,704 - MIT1002_02509 0.57 -0.1 2,798,741 - MIT1002_02509 0.61 +0.7 2,798,842 + MIT1002_02509 0.71 -1.7 2,798,860 - MIT1002_02509 0.73 +0.5 2,798,917 + MIT1002_02509 0.79 +0.0 2,798,965 + MIT1002_02509 0.83 +0.4 2,798,965 + MIT1002_02509 0.83 +0.6 2,799,107 - -0.3 2,799,122 + +0.2 2,799,130 - -0.4 2,799,130 - -0.2 2,799,222 + -0.2 2,799,260 - +1.1 2,799,528 - MIT1002_02510 0.51 +0.6 2,799,537 + MIT1002_02510 0.54 +1.1 2,799,704 + -0.2 2,799,712 - +0.3 2,799,716 + +0.5 2,799,726 + +0.1 2,799,772 - -0.4 2,799,899 - +0.8 2,800,001 - -0.4 2,800,020 + +0.7 2,800,028 - +0.6 2,800,115 + -0.5 2,800,118 - -1.2 2,800,120 + +0.1 2,800,128 - -1.1
Or see this region's nucleotide sequence