Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02153 and MIT1002_02154 are separated by 31 nucleotides MIT1002_02154 and MIT1002_02155 are separated by 180 nucleotides MIT1002_02155 and MIT1002_02156 are separated by 210 nucleotides
MIT1002_02153: MIT1002_02153 - Excinuclease ABC subunit C, at 2,409,293 to 2,411,110
_02153
MIT1002_02154: MIT1002_02154 - Response regulator UvrY, at 2,411,142 to 2,411,786
_02154
MIT1002_02155: MIT1002_02155 - Quercetin 2,3-dioxygenase, at 2,411,967 to 2,412,692
_02155
MIT1002_02156: MIT1002_02156 - Cystathionine beta-lyase PatB, at 2,412,903 to 2,414,366
_02156
Position (kb)
2411
2412
2413 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8 at 2411.051 kb on + strand at 2411.059 kb on - strand at 2411.114 kb on - strand at 2411.193 kb on - strand at 2411.236 kb on + strand, within MIT1002_02154 at 2411.242 kb on + strand, within MIT1002_02154 at 2411.400 kb on - strand, within MIT1002_02154 at 2411.746 kb on - strand at 2411.809 kb on - strand at 2411.858 kb on + strand at 2411.866 kb on + strand at 2411.866 kb on + strand at 2411.866 kb on + strand at 2411.874 kb on - strand at 2411.874 kb on - strand at 2411.874 kb on - strand at 2412.087 kb on + strand, within MIT1002_02155 at 2412.087 kb on + strand, within MIT1002_02155 at 2412.095 kb on - strand, within MIT1002_02155 at 2412.095 kb on - strand, within MIT1002_02155 at 2412.095 kb on - strand, within MIT1002_02155 at 2412.097 kb on + strand, within MIT1002_02155 at 2412.099 kb on + strand, within MIT1002_02155 at 2412.109 kb on - strand, within MIT1002_02155 at 2412.140 kb on + strand, within MIT1002_02155 at 2412.147 kb on + strand, within MIT1002_02155 at 2412.223 kb on + strand, within MIT1002_02155 at 2412.223 kb on + strand, within MIT1002_02155 at 2412.227 kb on - strand, within MIT1002_02155 at 2412.307 kb on + strand, within MIT1002_02155 at 2412.310 kb on - strand, within MIT1002_02155 at 2412.379 kb on - strand, within MIT1002_02155 at 2412.387 kb on - strand, within MIT1002_02155 at 2412.396 kb on + strand, within MIT1002_02155 at 2412.402 kb on + strand, within MIT1002_02155 at 2412.403 kb on - strand, within MIT1002_02155 at 2412.403 kb on - strand, within MIT1002_02155 at 2412.423 kb on - strand, within MIT1002_02155 at 2412.525 kb on - strand, within MIT1002_02155 at 2412.538 kb on + strand, within MIT1002_02155 at 2412.606 kb on + strand, within MIT1002_02155 at 2412.729 kb on + strand at 2412.731 kb on + strand at 2412.739 kb on - strand at 2412.739 kb on - strand at 2412.745 kb on + strand at 2412.753 kb on - strand at 2412.753 kb on - strand at 2412.976 kb on - strand at 2412.995 kb on - strand at 2413.044 kb on + strand at 2413.083 kb on + strand, within MIT1002_02156 at 2413.083 kb on + strand, within MIT1002_02156 at 2413.086 kb on - strand, within MIT1002_02156 at 2413.091 kb on - strand, within MIT1002_02156 at 2413.091 kb on - strand, within MIT1002_02156 at 2413.114 kb on + strand, within MIT1002_02156 at 2413.114 kb on + strand, within MIT1002_02156 at 2413.151 kb on + strand, within MIT1002_02156 at 2413.159 kb on - strand, within MIT1002_02156 at 2413.174 kb on + strand, within MIT1002_02156 at 2413.211 kb on + strand, within MIT1002_02156 at 2413.266 kb on + strand, within MIT1002_02156 at 2413.266 kb on + strand, within MIT1002_02156 at 2413.266 kb on + strand, within MIT1002_02156 at 2413.274 kb on - strand, within MIT1002_02156 at 2413.282 kb on - strand, within MIT1002_02156 at 2413.327 kb on + strand, within MIT1002_02156 at 2413.422 kb on + strand, within MIT1002_02156 at 2413.446 kb on + strand, within MIT1002_02156 at 2413.463 kb on + strand, within MIT1002_02156 at 2413.474 kb on - strand, within MIT1002_02156 at 2413.482 kb on - strand, within MIT1002_02156 at 2413.482 kb on - strand, within MIT1002_02156 at 2413.482 kb on - strand, within MIT1002_02156 at 2413.529 kb on + strand, within MIT1002_02156 at 2413.529 kb on + strand, within MIT1002_02156 at 2413.598 kb on + strand, within MIT1002_02156 at 2413.658 kb on - strand, within MIT1002_02156
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 2,411,051 + +0.4 2,411,059 - -1.9 2,411,114 - +0.8 2,411,193 - +8.1 2,411,236 + MIT1002_02154 0.15 +8.6 2,411,242 + MIT1002_02154 0.16 +7.5 2,411,400 - MIT1002_02154 0.40 +8.2 2,411,746 - +7.9 2,411,809 - +2.0 2,411,858 + +7.2 2,411,866 + +4.9 2,411,866 + +4.9 2,411,866 + +5.4 2,411,874 - +2.9 2,411,874 - -0.3 2,411,874 - +0.6 2,412,087 + MIT1002_02155 0.17 +0.4 2,412,087 + MIT1002_02155 0.17 -0.6 2,412,095 - MIT1002_02155 0.18 -0.5 2,412,095 - MIT1002_02155 0.18 -1.2 2,412,095 - MIT1002_02155 0.18 +0.8 2,412,097 + MIT1002_02155 0.18 +0.4 2,412,099 + MIT1002_02155 0.18 -0.1 2,412,109 - MIT1002_02155 0.20 +0.6 2,412,140 + MIT1002_02155 0.24 +0.3 2,412,147 + MIT1002_02155 0.25 +0.5 2,412,223 + MIT1002_02155 0.35 -1.0 2,412,223 + MIT1002_02155 0.35 -0.4 2,412,227 - MIT1002_02155 0.36 -0.7 2,412,307 + MIT1002_02155 0.47 +0.6 2,412,310 - MIT1002_02155 0.47 -0.8 2,412,379 - MIT1002_02155 0.57 -1.0 2,412,387 - MIT1002_02155 0.58 +0.4 2,412,396 + MIT1002_02155 0.59 +0.3 2,412,402 + MIT1002_02155 0.60 +0.1 2,412,403 - MIT1002_02155 0.60 +0.5 2,412,403 - MIT1002_02155 0.60 -0.3 2,412,423 - MIT1002_02155 0.63 +0.6 2,412,525 - MIT1002_02155 0.77 -0.5 2,412,538 + MIT1002_02155 0.79 -0.5 2,412,606 + MIT1002_02155 0.88 +0.9 2,412,729 + -2.7 2,412,731 + -1.9 2,412,739 - +0.3 2,412,739 - -1.7 2,412,745 + +0.0 2,412,753 - +0.5 2,412,753 - +0.1 2,412,976 - +0.6 2,412,995 - -0.4 2,413,044 + -1.7 2,413,083 + MIT1002_02156 0.12 -1.9 2,413,083 + MIT1002_02156 0.12 -0.5 2,413,086 - MIT1002_02156 0.12 +0.8 2,413,091 - MIT1002_02156 0.13 -0.5 2,413,091 - MIT1002_02156 0.13 +0.2 2,413,114 + MIT1002_02156 0.14 -0.5 2,413,114 + MIT1002_02156 0.14 -0.9 2,413,151 + MIT1002_02156 0.17 -0.4 2,413,159 - MIT1002_02156 0.17 +0.1 2,413,174 + MIT1002_02156 0.19 -0.5 2,413,211 + MIT1002_02156 0.21 -0.1 2,413,266 + MIT1002_02156 0.25 -0.7 2,413,266 + MIT1002_02156 0.25 +0.1 2,413,266 + MIT1002_02156 0.25 -0.8 2,413,274 - MIT1002_02156 0.25 -0.2 2,413,282 - MIT1002_02156 0.26 +0.9 2,413,327 + MIT1002_02156 0.29 -0.1 2,413,422 + MIT1002_02156 0.35 -0.7 2,413,446 + MIT1002_02156 0.37 +0.7 2,413,463 + MIT1002_02156 0.38 -0.4 2,413,474 - MIT1002_02156 0.39 +1.9 2,413,482 - MIT1002_02156 0.40 +0.5 2,413,482 - MIT1002_02156 0.40 +1.0 2,413,482 - MIT1002_02156 0.40 +0.4 2,413,529 + MIT1002_02156 0.43 -0.2 2,413,529 + MIT1002_02156 0.43 -0.6 2,413,598 + MIT1002_02156 0.47 -0.2 2,413,658 - MIT1002_02156 0.52 -0.7
Or see this region's nucleotide sequence