Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00809 and MIT1002_00810 are separated by 1 nucleotides MIT1002_00810 and MIT1002_00811 are separated by 75 nucleotides MIT1002_00811 and MIT1002_00812 are separated by 298 nucleotides MIT1002_00812 and MIT1002_00813 are separated by 12 nucleotides
MIT1002_00809: MIT1002_00809 - Sensor protein QseC, at 894,991 to 896,280
_00809
MIT1002_00810: MIT1002_00810 - Transcriptional regulatory protein BasR, at 896,282 to 896,947
_00810
MIT1002_00811: MIT1002_00811 - hypothetical protein, at 897,023 to 897,895
_00811
MIT1002_00812: MIT1002_00812 - 50S ribosomal protein L13, at 898,194 to 898,622
_00812
MIT1002_00813: MIT1002_00813 - 30S ribosomal protein S9, at 898,635 to 899,027
_00813
Position (kb)
897
898 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 896.028 kb on - strand, within MIT1002_00809 at 896.028 kb on - strand, within MIT1002_00809 at 896.028 kb on - strand, within MIT1002_00809 at 896.028 kb on - strand, within MIT1002_00809 at 896.051 kb on - strand, within MIT1002_00809 at 896.143 kb on - strand, within MIT1002_00809 at 896.143 kb on - strand, within MIT1002_00809 at 896.143 kb on - strand, within MIT1002_00809 at 896.171 kb on + strand at 896.190 kb on - strand at 896.202 kb on - strand at 896.208 kb on - strand at 896.245 kb on - strand at 896.323 kb on - strand at 896.374 kb on + strand, within MIT1002_00810 at 896.374 kb on + strand, within MIT1002_00810 at 896.441 kb on - strand, within MIT1002_00810 at 896.531 kb on + strand, within MIT1002_00810 at 896.596 kb on - strand, within MIT1002_00810 at 896.596 kb on - strand, within MIT1002_00810 at 896.608 kb on + strand, within MIT1002_00810 at 896.616 kb on - strand, within MIT1002_00810 at 896.644 kb on + strand, within MIT1002_00810 at 896.664 kb on - strand, within MIT1002_00810 at 896.697 kb on + strand, within MIT1002_00810 at 896.720 kb on - strand, within MIT1002_00810 at 896.786 kb on - strand, within MIT1002_00810 at 896.868 kb on + strand, within MIT1002_00810 at 896.896 kb on + strand at 896.904 kb on - strand at 896.912 kb on - strand at 896.936 kb on + strand at 896.936 kb on + strand at 897.013 kb on + strand at 897.013 kb on - strand at 897.020 kb on + strand at 897.070 kb on + strand at 897.076 kb on - strand at 897.105 kb on + strand at 897.175 kb on + strand, within MIT1002_00811 at 897.183 kb on - strand, within MIT1002_00811 at 897.220 kb on - strand, within MIT1002_00811 at 897.229 kb on + strand, within MIT1002_00811 at 897.261 kb on - strand, within MIT1002_00811 at 897.283 kb on + strand, within MIT1002_00811 at 897.288 kb on + strand, within MIT1002_00811 at 897.308 kb on + strand, within MIT1002_00811 at 897.316 kb on + strand, within MIT1002_00811 at 897.316 kb on + strand, within MIT1002_00811 at 897.321 kb on + strand, within MIT1002_00811 at 897.417 kb on - strand, within MIT1002_00811 at 897.423 kb on + strand, within MIT1002_00811 at 897.446 kb on - strand, within MIT1002_00811 at 897.456 kb on + strand, within MIT1002_00811 at 897.458 kb on - strand, within MIT1002_00811 at 897.459 kb on - strand, within MIT1002_00811 at 897.503 kb on + strand, within MIT1002_00811 at 897.531 kb on - strand, within MIT1002_00811 at 897.706 kb on - strand, within MIT1002_00811 at 897.706 kb on - strand, within MIT1002_00811 at 897.739 kb on + strand, within MIT1002_00811 at 897.739 kb on + strand, within MIT1002_00811 at 897.739 kb on + strand, within MIT1002_00811 at 897.739 kb on + strand, within MIT1002_00811 at 897.756 kb on + strand, within MIT1002_00811 at 897.783 kb on + strand, within MIT1002_00811 at 897.788 kb on + strand, within MIT1002_00811 at 897.788 kb on + strand, within MIT1002_00811 at 897.796 kb on - strand, within MIT1002_00811 at 897.834 kb on + strand at 897.836 kb on + strand at 897.840 kb on - strand at 897.842 kb on - strand at 897.850 kb on - strand at 897.855 kb on + strand at 897.856 kb on + strand at 897.871 kb on + strand at 897.950 kb on - strand at 897.951 kb on + strand at 897.951 kb on + strand at 897.971 kb on - strand at 897.971 kb on - strand at 897.971 kb on - strand at 897.971 kb on - strand at 897.971 kb on - strand at 898.020 kb on + strand at 898.028 kb on - strand at 898.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 896,028 - MIT1002_00809 0.80 -0.1 896,028 - MIT1002_00809 0.80 +1.6 896,028 - MIT1002_00809 0.80 -1.1 896,028 - MIT1002_00809 0.80 +0.3 896,051 - MIT1002_00809 0.82 +1.1 896,143 - MIT1002_00809 0.89 -0.8 896,143 - MIT1002_00809 0.89 -0.5 896,143 - MIT1002_00809 0.89 -0.6 896,171 + -0.1 896,190 - -0.3 896,202 - +0.8 896,208 - -0.9 896,245 - -0.5 896,323 - +0.2 896,374 + MIT1002_00810 0.14 -0.5 896,374 + MIT1002_00810 0.14 -1.1 896,441 - MIT1002_00810 0.24 -0.3 896,531 + MIT1002_00810 0.37 -0.3 896,596 - MIT1002_00810 0.47 -0.2 896,596 - MIT1002_00810 0.47 +0.8 896,608 + MIT1002_00810 0.49 -0.6 896,616 - MIT1002_00810 0.50 +0.5 896,644 + MIT1002_00810 0.54 -1.4 896,664 - MIT1002_00810 0.57 -0.4 896,697 + MIT1002_00810 0.62 -0.5 896,720 - MIT1002_00810 0.66 +0.1 896,786 - MIT1002_00810 0.76 +0.6 896,868 + MIT1002_00810 0.88 +7.7 896,896 + -0.2 896,904 - -0.8 896,912 - +2.2 896,936 + -0.2 896,936 + -0.1 897,013 + +0.6 897,013 - +0.7 897,020 + -0.0 897,070 + -2.5 897,076 - -0.1 897,105 + +0.4 897,175 + MIT1002_00811 0.17 +0.9 897,183 - MIT1002_00811 0.18 -1.6 897,220 - MIT1002_00811 0.23 +0.1 897,229 + MIT1002_00811 0.24 -0.0 897,261 - MIT1002_00811 0.27 +0.0 897,283 + MIT1002_00811 0.30 +1.0 897,288 + MIT1002_00811 0.30 -0.1 897,308 + MIT1002_00811 0.33 +1.3 897,316 + MIT1002_00811 0.34 +0.0 897,316 + MIT1002_00811 0.34 -0.6 897,321 + MIT1002_00811 0.34 +0.1 897,417 - MIT1002_00811 0.45 +0.6 897,423 + MIT1002_00811 0.46 +0.4 897,446 - MIT1002_00811 0.48 +1.1 897,456 + MIT1002_00811 0.50 +0.3 897,458 - MIT1002_00811 0.50 -0.5 897,459 - MIT1002_00811 0.50 +1.2 897,503 + MIT1002_00811 0.55 +1.0 897,531 - MIT1002_00811 0.58 -1.0 897,706 - MIT1002_00811 0.78 -0.6 897,706 - MIT1002_00811 0.78 +0.4 897,739 + MIT1002_00811 0.82 -1.3 897,739 + MIT1002_00811 0.82 +0.4 897,739 + MIT1002_00811 0.82 -2.0 897,739 + MIT1002_00811 0.82 +0.3 897,756 + MIT1002_00811 0.84 -0.5 897,783 + MIT1002_00811 0.87 +0.6 897,788 + MIT1002_00811 0.88 +0.4 897,788 + MIT1002_00811 0.88 +0.1 897,796 - MIT1002_00811 0.89 -0.1 897,834 + +0.6 897,836 + +1.0 897,840 - -0.7 897,842 - +0.2 897,850 - +0.6 897,855 + -1.5 897,856 + -0.8 897,871 + -0.4 897,950 - +0.2 897,951 + -1.6 897,951 + -2.1 897,971 - -0.5 897,971 - -0.2 897,971 - +0.6 897,971 - +0.1 897,971 - -1.2 898,020 + -0.3 898,028 - -0.3 898,081 + +0.2
Or see this region's nucleotide sequence