Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00556 and MIT1002_00557 are separated by 52 nucleotides MIT1002_00557 and MIT1002_00558 are separated by 5 nucleotides MIT1002_00558 and MIT1002_00559 are separated by 66 nucleotides MIT1002_00559 and MIT1002_00560 are separated by 161 nucleotides MIT1002_00560 and MIT1002_00561 are separated by 40 nucleotides
MIT1002_00556: MIT1002_00556 - putative N-acetyltransferase YsnE, at 604,560 to 605,015
_00556
MIT1002_00557: MIT1002_00557 - ribosomal-protein-alanine acetyltransferase, at 605,068 to 605,526
_00557
MIT1002_00558: MIT1002_00558 - hypothetical protein, at 605,532 to 605,864
_00558
MIT1002_00559: MIT1002_00559 - Gram-negative bacterial tonB protein, at 605,931 to 606,365
_00559
MIT1002_00560: MIT1002_00560 - DNA-binding transcriptional activator PspC, at 606,527 to 606,784
_00560
MIT1002_00561: MIT1002_00561 - thiol-disulfide oxidoreductase, at 606,825 to 607,364
_00561
Position (kb) 605
606
607 Strain fitness (log2 ratio) -2
-1
0
1
2 at 605.018 kb on + strand at 605.018 kb on + strand at 605.025 kb on + strand at 605.036 kb on + strand at 605.036 kb on + strand at 605.039 kb on - strand at 605.072 kb on - strand at 605.082 kb on - strand at 605.082 kb on - strand at 605.082 kb on - strand at 605.088 kb on - strand at 605.180 kb on - strand, within MIT1002_00557 at 605.192 kb on + strand, within MIT1002_00557 at 605.192 kb on + strand, within MIT1002_00557 at 605.205 kb on + strand, within MIT1002_00557 at 605.239 kb on + strand, within MIT1002_00557 at 605.239 kb on + strand, within MIT1002_00557 at 605.239 kb on + strand, within MIT1002_00557 at 605.242 kb on - strand, within MIT1002_00557 at 605.246 kb on + strand, within MIT1002_00557 at 605.246 kb on + strand, within MIT1002_00557 at 605.246 kb on + strand, within MIT1002_00557 at 605.246 kb on + strand, within MIT1002_00557 at 605.246 kb on + strand, within MIT1002_00557 at 605.247 kb on - strand, within MIT1002_00557 at 605.247 kb on - strand, within MIT1002_00557 at 605.254 kb on - strand, within MIT1002_00557 at 605.254 kb on - strand, within MIT1002_00557 at 605.261 kb on - strand, within MIT1002_00557 at 605.263 kb on + strand, within MIT1002_00557 at 605.271 kb on + strand, within MIT1002_00557 at 605.271 kb on - strand, within MIT1002_00557 at 605.279 kb on - strand, within MIT1002_00557 at 605.286 kb on + strand, within MIT1002_00557 at 605.288 kb on + strand, within MIT1002_00557 at 605.294 kb on - strand, within MIT1002_00557 at 605.296 kb on - strand, within MIT1002_00557 at 605.346 kb on - strand, within MIT1002_00557 at 605.364 kb on + strand, within MIT1002_00557 at 605.413 kb on - strand, within MIT1002_00557 at 605.428 kb on - strand, within MIT1002_00557 at 605.704 kb on + strand, within MIT1002_00558 at 605.718 kb on - strand, within MIT1002_00558 at 605.772 kb on - strand, within MIT1002_00558 at 605.794 kb on - strand, within MIT1002_00558 at 605.833 kb on - strand at 605.866 kb on + strand at 605.874 kb on - strand at 605.981 kb on + strand, within MIT1002_00559 at 606.028 kb on - strand, within MIT1002_00559 at 606.157 kb on - strand, within MIT1002_00559 at 606.276 kb on - strand, within MIT1002_00559 at 606.284 kb on + strand, within MIT1002_00559 at 606.292 kb on - strand, within MIT1002_00559 at 606.302 kb on - strand, within MIT1002_00559 at 606.439 kb on + strand at 606.439 kb on + strand at 606.451 kb on + strand at 606.592 kb on + strand, within MIT1002_00560 at 606.592 kb on + strand, within MIT1002_00560 at 606.702 kb on - strand, within MIT1002_00560 at 606.710 kb on - strand, within MIT1002_00560 at 606.781 kb on + strand at 606.907 kb on + strand, within MIT1002_00561 at 606.950 kb on + strand, within MIT1002_00561 at 607.066 kb on - strand, within MIT1002_00561 at 607.066 kb on - strand, within MIT1002_00561 at 607.122 kb on - strand, within MIT1002_00561 at 607.122 kb on - strand, within MIT1002_00561 at 607.198 kb on - strand, within MIT1002_00561 at 607.216 kb on + strand, within MIT1002_00561 at 607.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 605,018 + +0.4 605,018 + +0.7 605,025 + -0.9 605,036 + +0.5 605,036 + +0.8 605,039 - +0.5 605,072 - +0.8 605,082 - -1.1 605,082 - -0.6 605,082 - -0.3 605,088 - -0.2 605,180 - MIT1002_00557 0.24 +0.6 605,192 + MIT1002_00557 0.27 +0.7 605,192 + MIT1002_00557 0.27 +0.1 605,205 + MIT1002_00557 0.30 +0.1 605,239 + MIT1002_00557 0.37 +1.3 605,239 + MIT1002_00557 0.37 +0.4 605,239 + MIT1002_00557 0.37 +0.2 605,242 - MIT1002_00557 0.38 +0.3 605,246 + MIT1002_00557 0.39 -1.3 605,246 + MIT1002_00557 0.39 +0.6 605,246 + MIT1002_00557 0.39 -0.4 605,246 + MIT1002_00557 0.39 -0.8 605,246 + MIT1002_00557 0.39 +0.5 605,247 - MIT1002_00557 0.39 +0.1 605,247 - MIT1002_00557 0.39 +1.3 605,254 - MIT1002_00557 0.41 +0.7 605,254 - MIT1002_00557 0.41 -0.2 605,261 - MIT1002_00557 0.42 +0.1 605,263 + MIT1002_00557 0.42 +1.1 605,271 + MIT1002_00557 0.44 -1.9 605,271 - MIT1002_00557 0.44 +0.0 605,279 - MIT1002_00557 0.46 +0.3 605,286 + MIT1002_00557 0.47 +1.2 605,288 + MIT1002_00557 0.48 +2.0 605,294 - MIT1002_00557 0.49 -0.3 605,296 - MIT1002_00557 0.50 -0.7 605,346 - MIT1002_00557 0.61 -0.3 605,364 + MIT1002_00557 0.64 -0.1 605,413 - MIT1002_00557 0.75 +0.1 605,428 - MIT1002_00557 0.78 -0.5 605,704 + MIT1002_00558 0.52 +0.8 605,718 - MIT1002_00558 0.56 +0.8 605,772 - MIT1002_00558 0.72 +0.2 605,794 - MIT1002_00558 0.79 -0.6 605,833 - +0.1 605,866 + +0.2 605,874 - -1.0 605,981 + MIT1002_00559 0.11 +0.7 606,028 - MIT1002_00559 0.22 +0.4 606,157 - MIT1002_00559 0.52 +0.1 606,276 - MIT1002_00559 0.79 -0.6 606,284 + MIT1002_00559 0.81 -1.8 606,292 - MIT1002_00559 0.83 +0.2 606,302 - MIT1002_00559 0.85 +0.1 606,439 + -0.1 606,439 + -0.2 606,451 + -0.3 606,592 + MIT1002_00560 0.25 -0.7 606,592 + MIT1002_00560 0.25 -0.6 606,702 - MIT1002_00560 0.68 -1.4 606,710 - MIT1002_00560 0.71 -1.7 606,781 + -0.8 606,907 + MIT1002_00561 0.15 -0.6 606,950 + MIT1002_00561 0.23 -0.3 607,066 - MIT1002_00561 0.45 +0.1 607,066 - MIT1002_00561 0.45 -1.0 607,122 - MIT1002_00561 0.55 +2.3 607,122 - MIT1002_00561 0.55 -0.8 607,198 - MIT1002_00561 0.69 -1.6 607,216 + MIT1002_00561 0.72 -1.8 607,314 - +1.1
Or see this region's nucleotide sequence