Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00143 and MIT1002_00144 are separated by 135 nucleotides MIT1002_00144 and MIT1002_00145 are separated by 132 nucleotides MIT1002_00145 and MIT1002_00146 are separated by 94 nucleotides MIT1002_00146 and MIT1002_00147 are separated by 105 nucleotides
MIT1002_00143: MIT1002_00143 - hypothetical protein, at 155,203 to 156,645
_00143
MIT1002_00144: MIT1002_00144 - GTP cyclohydrolase-2, at 156,781 to 157,395
_00144
MIT1002_00145: MIT1002_00145 - Cyclic di-GMP phosphodiesterase Gmr, at 157,528 to 160,368
_00145
MIT1002_00146: MIT1002_00146 - hypothetical protein, at 160,463 to 160,780
_00146
MIT1002_00147: MIT1002_00147 - Glutathione S-transferase GST-6.0, at 160,886 to 161,554
_00147
Position (kb) 157
158
159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 156.540 kb on + strand at 156.602 kb on + strand at 156.695 kb on + strand at 156.704 kb on + strand at 156.705 kb on + strand at 156.712 kb on - strand at 156.773 kb on + strand at 156.773 kb on + strand at 157.495 kb on - strand at 157.569 kb on + strand at 157.570 kb on + strand at 157.619 kb on - strand at 157.666 kb on - strand at 157.679 kb on - strand at 157.700 kb on - strand at 157.737 kb on - strand at 157.834 kb on - strand, within MIT1002_00145 at 157.844 kb on - strand, within MIT1002_00145 at 157.854 kb on + strand, within MIT1002_00145 at 157.904 kb on + strand, within MIT1002_00145 at 157.904 kb on + strand, within MIT1002_00145 at 157.990 kb on + strand, within MIT1002_00145 at 158.003 kb on - strand, within MIT1002_00145 at 158.019 kb on - strand, within MIT1002_00145 at 158.019 kb on - strand, within MIT1002_00145 at 158.019 kb on - strand, within MIT1002_00145 at 158.048 kb on + strand, within MIT1002_00145 at 158.172 kb on + strand, within MIT1002_00145 at 158.182 kb on - strand, within MIT1002_00145 at 158.229 kb on + strand, within MIT1002_00145 at 158.264 kb on + strand, within MIT1002_00145 at 158.349 kb on - strand, within MIT1002_00145 at 158.386 kb on + strand, within MIT1002_00145 at 158.399 kb on - strand, within MIT1002_00145 at 158.399 kb on - strand, within MIT1002_00145 at 158.403 kb on + strand, within MIT1002_00145 at 158.403 kb on + strand, within MIT1002_00145 at 158.479 kb on + strand, within MIT1002_00145 at 158.479 kb on + strand, within MIT1002_00145 at 158.479 kb on + strand, within MIT1002_00145 at 158.484 kb on + strand, within MIT1002_00145 at 158.484 kb on + strand, within MIT1002_00145 at 158.487 kb on - strand, within MIT1002_00145 at 158.487 kb on - strand, within MIT1002_00145 at 158.492 kb on - strand, within MIT1002_00145 at 158.512 kb on + strand, within MIT1002_00145 at 158.512 kb on + strand, within MIT1002_00145 at 158.515 kb on - strand, within MIT1002_00145 at 158.564 kb on + strand, within MIT1002_00145 at 158.564 kb on + strand, within MIT1002_00145 at 158.572 kb on - strand, within MIT1002_00145 at 158.659 kb on + strand, within MIT1002_00145 at 158.667 kb on - strand, within MIT1002_00145 at 158.673 kb on + strand, within MIT1002_00145 at 158.717 kb on - strand, within MIT1002_00145 at 158.765 kb on + strand, within MIT1002_00145 at 158.767 kb on + strand, within MIT1002_00145 at 158.767 kb on + strand, within MIT1002_00145 at 158.767 kb on + strand, within MIT1002_00145 at 158.773 kb on - strand, within MIT1002_00145 at 158.773 kb on - strand, within MIT1002_00145 at 158.773 kb on - strand, within MIT1002_00145 at 158.796 kb on - strand, within MIT1002_00145 at 158.796 kb on - strand, within MIT1002_00145 at 158.796 kb on - strand, within MIT1002_00145 at 158.796 kb on - strand, within MIT1002_00145 at 158.798 kb on + strand, within MIT1002_00145 at 158.811 kb on + strand, within MIT1002_00145 at 158.865 kb on - strand, within MIT1002_00145 at 158.921 kb on - strand, within MIT1002_00145 at 158.951 kb on - strand, within MIT1002_00145 at 158.996 kb on - strand, within MIT1002_00145 at 159.006 kb on - strand, within MIT1002_00145 at 159.067 kb on + strand, within MIT1002_00145 at 159.122 kb on + strand, within MIT1002_00145 at 159.133 kb on - strand, within MIT1002_00145 at 159.135 kb on - strand, within MIT1002_00145 at 159.246 kb on + strand, within MIT1002_00145 at 159.262 kb on + strand, within MIT1002_00145 at 159.294 kb on + strand, within MIT1002_00145 at 159.327 kb on + strand, within MIT1002_00145 at 159.334 kb on + strand, within MIT1002_00145 at 159.336 kb on - strand, within MIT1002_00145 at 159.383 kb on - strand, within MIT1002_00145 at 159.398 kb on - strand, within MIT1002_00145 at 159.411 kb on - strand, within MIT1002_00145 at 159.439 kb on + strand, within MIT1002_00145 at 159.527 kb on - strand, within MIT1002_00145 at 159.546 kb on + strand, within MIT1002_00145 at 159.546 kb on + strand, within MIT1002_00145 at 159.626 kb on + strand, within MIT1002_00145 at 159.649 kb on + strand, within MIT1002_00145 at 159.654 kb on + strand, within MIT1002_00145 at 159.654 kb on + strand, within MIT1002_00145 at 159.657 kb on - strand, within MIT1002_00145 at 159.657 kb on - strand, within MIT1002_00145 at 159.657 kb on - strand, within MIT1002_00145 at 159.671 kb on + strand, within MIT1002_00145 at 159.679 kb on - strand, within MIT1002_00145 at 159.683 kb on + strand, within MIT1002_00145 at 159.683 kb on + strand, within MIT1002_00145 at 159.689 kb on - strand, within MIT1002_00145 at 159.801 kb on + strand, within MIT1002_00145 at 159.801 kb on + strand, within MIT1002_00145 at 159.854 kb on + strand, within MIT1002_00145 at 159.866 kb on - strand, within MIT1002_00145 at 159.885 kb on - strand, within MIT1002_00145 at 159.887 kb on - strand, within MIT1002_00145 at 159.930 kb on + strand, within MIT1002_00145 at 159.930 kb on + strand, within MIT1002_00145 at 159.930 kb on + strand, within MIT1002_00145 at 159.937 kb on - strand, within MIT1002_00145 at 159.938 kb on - strand, within MIT1002_00145 at 159.938 kb on - strand, within MIT1002_00145 at 159.993 kb on - strand, within MIT1002_00145 at 160.041 kb on - strand, within MIT1002_00145 at 160.084 kb on - strand, within MIT1002_00145 at 160.084 kb on - strand, within MIT1002_00145 at 160.114 kb on + strand at 160.119 kb on + strand at 160.124 kb on + strand at 160.130 kb on - strand at 160.132 kb on - strand at 160.223 kb on + strand at 160.223 kb on + strand at 160.226 kb on - strand at 160.231 kb on - strand at 160.353 kb on + strand at 160.454 kb on + strand at 160.454 kb on + strand at 160.462 kb on - strand at 160.462 kb on - strand at 160.496 kb on - strand, within MIT1002_00146 at 160.637 kb on - strand, within MIT1002_00146 at 160.674 kb on - strand, within MIT1002_00146 at 160.674 kb on - strand, within MIT1002_00146 at 160.678 kb on + strand, within MIT1002_00146 at 160.700 kb on + strand, within MIT1002_00146 at 160.702 kb on - strand, within MIT1002_00146 at 160.725 kb on - strand, within MIT1002_00146 at 160.803 kb on + strand at 160.828 kb on + strand at 160.830 kb on + strand at 160.832 kb on + strand at 160.838 kb on - strand at 160.954 kb on - strand, within MIT1002_00147 at 161.093 kb on + strand, within MIT1002_00147 at 161.093 kb on + strand, within MIT1002_00147 at 161.101 kb on - strand, within MIT1002_00147 at 161.146 kb on - strand, within MIT1002_00147 at 161.154 kb on + strand, within MIT1002_00147 at 161.220 kb on + strand, within MIT1002_00147 at 161.220 kb on + strand, within MIT1002_00147 at 161.346 kb on + strand, within MIT1002_00147 at 161.354 kb on - strand, within MIT1002_00147
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 156,540 + +0.9 156,602 + -0.2 156,695 + -0.3 156,704 + +0.2 156,705 + +0.2 156,712 - -0.3 156,773 + -0.0 156,773 + -0.2 157,495 - -0.9 157,569 + +0.2 157,570 + -0.3 157,619 - +0.2 157,666 - +0.1 157,679 - -0.4 157,700 - -0.7 157,737 - +0.6 157,834 - MIT1002_00145 0.11 +1.6 157,844 - MIT1002_00145 0.11 -0.3 157,854 + MIT1002_00145 0.11 -0.4 157,904 + MIT1002_00145 0.13 -1.3 157,904 + MIT1002_00145 0.13 +1.2 157,990 + MIT1002_00145 0.16 +2.0 158,003 - MIT1002_00145 0.17 +0.7 158,019 - MIT1002_00145 0.17 -0.5 158,019 - MIT1002_00145 0.17 -0.6 158,019 - MIT1002_00145 0.17 -1.1 158,048 + MIT1002_00145 0.18 -0.6 158,172 + MIT1002_00145 0.23 -0.7 158,182 - MIT1002_00145 0.23 -0.5 158,229 + MIT1002_00145 0.25 +0.4 158,264 + MIT1002_00145 0.26 -0.4 158,349 - MIT1002_00145 0.29 -0.2 158,386 + MIT1002_00145 0.30 -0.0 158,399 - MIT1002_00145 0.31 +0.1 158,399 - MIT1002_00145 0.31 +0.4 158,403 + MIT1002_00145 0.31 -1.2 158,403 + MIT1002_00145 0.31 +0.7 158,479 + MIT1002_00145 0.33 -0.5 158,479 + MIT1002_00145 0.33 -0.8 158,479 + MIT1002_00145 0.33 -1.1 158,484 + MIT1002_00145 0.34 +1.0 158,484 + MIT1002_00145 0.34 -0.3 158,487 - MIT1002_00145 0.34 -1.0 158,487 - MIT1002_00145 0.34 +0.2 158,492 - MIT1002_00145 0.34 -0.7 158,512 + MIT1002_00145 0.35 +0.7 158,512 + MIT1002_00145 0.35 -1.7 158,515 - MIT1002_00145 0.35 -0.6 158,564 + MIT1002_00145 0.36 -1.8 158,564 + MIT1002_00145 0.36 -1.3 158,572 - MIT1002_00145 0.37 -0.5 158,659 + MIT1002_00145 0.40 -0.4 158,667 - MIT1002_00145 0.40 +0.2 158,673 + MIT1002_00145 0.40 +0.2 158,717 - MIT1002_00145 0.42 +0.0 158,765 + MIT1002_00145 0.44 -0.6 158,767 + MIT1002_00145 0.44 +0.2 158,767 + MIT1002_00145 0.44 -1.8 158,767 + MIT1002_00145 0.44 +0.0 158,773 - MIT1002_00145 0.44 -1.1 158,773 - MIT1002_00145 0.44 -0.1 158,773 - MIT1002_00145 0.44 -0.1 158,796 - MIT1002_00145 0.45 +0.9 158,796 - MIT1002_00145 0.45 -0.8 158,796 - MIT1002_00145 0.45 -0.2 158,796 - MIT1002_00145 0.45 +0.2 158,798 + MIT1002_00145 0.45 -1.8 158,811 + MIT1002_00145 0.45 +0.6 158,865 - MIT1002_00145 0.47 -1.0 158,921 - MIT1002_00145 0.49 -0.6 158,951 - MIT1002_00145 0.50 +0.1 158,996 - MIT1002_00145 0.52 -0.3 159,006 - MIT1002_00145 0.52 -0.6 159,067 + MIT1002_00145 0.54 +1.5 159,122 + MIT1002_00145 0.56 +1.3 159,133 - MIT1002_00145 0.56 -1.7 159,135 - MIT1002_00145 0.57 +0.1 159,246 + MIT1002_00145 0.60 +0.0 159,262 + MIT1002_00145 0.61 -1.8 159,294 + MIT1002_00145 0.62 +0.1 159,327 + MIT1002_00145 0.63 +0.2 159,334 + MIT1002_00145 0.64 -0.3 159,336 - MIT1002_00145 0.64 +0.5 159,383 - MIT1002_00145 0.65 -0.7 159,398 - MIT1002_00145 0.66 -0.4 159,411 - MIT1002_00145 0.66 +0.2 159,439 + MIT1002_00145 0.67 +0.7 159,527 - MIT1002_00145 0.70 -0.4 159,546 + MIT1002_00145 0.71 -0.3 159,546 + MIT1002_00145 0.71 -0.8 159,626 + MIT1002_00145 0.74 -1.2 159,649 + MIT1002_00145 0.75 +0.2 159,654 + MIT1002_00145 0.75 -1.0 159,654 + MIT1002_00145 0.75 -0.1 159,657 - MIT1002_00145 0.75 -2.3 159,657 - MIT1002_00145 0.75 +0.0 159,657 - MIT1002_00145 0.75 -0.4 159,671 + MIT1002_00145 0.75 -1.4 159,679 - MIT1002_00145 0.76 -0.1 159,683 + MIT1002_00145 0.76 -1.4 159,683 + MIT1002_00145 0.76 -0.8 159,689 - MIT1002_00145 0.76 -2.6 159,801 + MIT1002_00145 0.80 -2.2 159,801 + MIT1002_00145 0.80 -0.8 159,854 + MIT1002_00145 0.82 -1.5 159,866 - MIT1002_00145 0.82 -0.8 159,885 - MIT1002_00145 0.83 -0.4 159,887 - MIT1002_00145 0.83 -0.6 159,930 + MIT1002_00145 0.85 -0.8 159,930 + MIT1002_00145 0.85 -1.6 159,930 + MIT1002_00145 0.85 -0.3 159,937 - MIT1002_00145 0.85 -0.3 159,938 - MIT1002_00145 0.85 +0.3 159,938 - MIT1002_00145 0.85 -0.1 159,993 - MIT1002_00145 0.87 -0.5 160,041 - MIT1002_00145 0.88 +0.6 160,084 - MIT1002_00145 0.90 -0.3 160,084 - MIT1002_00145 0.90 -1.3 160,114 + -0.3 160,119 + +0.1 160,124 + +0.2 160,130 - +2.3 160,132 - -0.6 160,223 + -0.8 160,223 + -0.3 160,226 - -0.6 160,231 - +1.6 160,353 + -1.2 160,454 + -0.3 160,454 + +0.6 160,462 - +0.3 160,462 - +0.6 160,496 - MIT1002_00146 0.10 +0.5 160,637 - MIT1002_00146 0.55 -0.6 160,674 - MIT1002_00146 0.66 +0.5 160,674 - MIT1002_00146 0.66 -0.5 160,678 + MIT1002_00146 0.68 +0.0 160,700 + MIT1002_00146 0.75 +1.0 160,702 - MIT1002_00146 0.75 +0.9 160,725 - MIT1002_00146 0.82 -0.6 160,803 + +8.3 160,828 + +1.6 160,830 + +0.2 160,832 + +0.1 160,838 - +1.0 160,954 - MIT1002_00147 0.10 +0.1 161,093 + MIT1002_00147 0.31 -0.4 161,093 + MIT1002_00147 0.31 -0.2 161,101 - MIT1002_00147 0.32 -0.8 161,146 - MIT1002_00147 0.39 +0.3 161,154 + MIT1002_00147 0.40 -0.1 161,220 + MIT1002_00147 0.50 +0.3 161,220 + MIT1002_00147 0.50 -0.1 161,346 + MIT1002_00147 0.69 +0.3 161,354 - MIT1002_00147 0.70 -0.7
Or see this region's nucleotide sequence