Experiment: Filtered_supernatant_MT80_in_LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS01970 and QEN71_RS01975 are separated by 202 nucleotides QEN71_RS01975 and QEN71_RS01980 overlap by 4 nucleotides QEN71_RS01980 and QEN71_RS01985 are separated by 9 nucleotides
QEN71_RS01970: QEN71_RS01970 - IS481 family transposase, at 434,918 to 436,057
_RS01970
QEN71_RS01975: QEN71_RS01975 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 436,260 to 437,084
_RS01975
QEN71_RS01980: QEN71_RS01980 - 5'-nucleotidase, at 437,081 to 437,983
_RS01980
QEN71_RS01985: QEN71_RS01985 - threonine ammonia-lyase, biosynthetic, at 437,993 to 439,516
_RS01985
Position (kb)
436
437
438 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 436.182 kb on + strand at 436.261 kb on + strand at 436.261 kb on + strand at 436.261 kb on + strand at 436.284 kb on - strand at 436.301 kb on + strand at 436.325 kb on + strand at 436.325 kb on + strand at 436.348 kb on + strand, within QEN71_RS01975 at 436.357 kb on + strand, within QEN71_RS01975 at 436.357 kb on + strand, within QEN71_RS01975 at 436.357 kb on + strand, within QEN71_RS01975 at 436.358 kb on - strand, within QEN71_RS01975 at 436.371 kb on - strand, within QEN71_RS01975 at 436.454 kb on + strand, within QEN71_RS01975 at 436.454 kb on + strand, within QEN71_RS01975 at 436.454 kb on + strand, within QEN71_RS01975 at 436.455 kb on - strand, within QEN71_RS01975 at 436.455 kb on - strand, within QEN71_RS01975 at 436.459 kb on + strand, within QEN71_RS01975 at 436.459 kb on + strand, within QEN71_RS01975 at 436.459 kb on + strand, within QEN71_RS01975 at 436.459 kb on + strand, within QEN71_RS01975 at 436.459 kb on + strand, within QEN71_RS01975 at 436.460 kb on - strand, within QEN71_RS01975 at 436.460 kb on - strand, within QEN71_RS01975 at 436.460 kb on - strand, within QEN71_RS01975 at 436.460 kb on - strand, within QEN71_RS01975 at 436.498 kb on + strand, within QEN71_RS01975 at 436.498 kb on + strand, within QEN71_RS01975 at 436.498 kb on + strand, within QEN71_RS01975 at 436.498 kb on + strand, within QEN71_RS01975 at 436.498 kb on + strand, within QEN71_RS01975 at 436.498 kb on + strand, within QEN71_RS01975 at 436.499 kb on - strand, within QEN71_RS01975 at 436.499 kb on - strand, within QEN71_RS01975 at 436.499 kb on - strand, within QEN71_RS01975 at 436.553 kb on + strand, within QEN71_RS01975 at 436.553 kb on + strand, within QEN71_RS01975 at 436.553 kb on + strand, within QEN71_RS01975 at 436.554 kb on - strand, within QEN71_RS01975 at 436.554 kb on - strand, within QEN71_RS01975 at 436.630 kb on + strand, within QEN71_RS01975 at 436.630 kb on + strand, within QEN71_RS01975 at 436.630 kb on + strand, within QEN71_RS01975 at 436.630 kb on + strand, within QEN71_RS01975 at 436.630 kb on + strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.631 kb on - strand, within QEN71_RS01975 at 436.822 kb on + strand, within QEN71_RS01975 at 436.822 kb on + strand, within QEN71_RS01975 at 436.823 kb on - strand, within QEN71_RS01975 at 436.850 kb on + strand, within QEN71_RS01975 at 436.850 kb on + strand, within QEN71_RS01975 at 436.851 kb on - strand, within QEN71_RS01975 at 436.873 kb on + strand, within QEN71_RS01975 at 436.873 kb on + strand, within QEN71_RS01975 at 436.873 kb on + strand, within QEN71_RS01975 at 436.873 kb on + strand, within QEN71_RS01975 at 436.873 kb on + strand, within QEN71_RS01975 at 436.874 kb on - strand, within QEN71_RS01975 at 436.874 kb on - strand, within QEN71_RS01975 at 436.874 kb on - strand, within QEN71_RS01975 at 436.874 kb on - strand, within QEN71_RS01975 at 436.930 kb on + strand, within QEN71_RS01975 at 436.930 kb on + strand, within QEN71_RS01975 at 436.930 kb on + strand, within QEN71_RS01975 at 436.930 kb on + strand, within QEN71_RS01975 at 437.032 kb on + strand at 437.032 kb on + strand at 437.032 kb on + strand at 437.033 kb on - strand at 437.033 kb on - strand at 437.044 kb on + strand at 437.044 kb on + strand at 437.045 kb on - strand at 437.045 kb on - strand at 437.444 kb on + strand, within QEN71_RS01980 at 437.445 kb on - strand, within QEN71_RS01980 at 437.486 kb on + strand, within QEN71_RS01980 at 437.486 kb on + strand, within QEN71_RS01980 at 437.487 kb on - strand, within QEN71_RS01980 at 437.487 kb on - strand, within QEN71_RS01980 at 437.529 kb on + strand, within QEN71_RS01980 at 437.530 kb on - strand, within QEN71_RS01980 at 437.533 kb on + strand, within QEN71_RS01980 at 437.533 kb on + strand, within QEN71_RS01980 at 437.533 kb on + strand, within QEN71_RS01980 at 437.533 kb on + strand, within QEN71_RS01980 at 437.652 kb on + strand, within QEN71_RS01980 at 437.652 kb on + strand, within QEN71_RS01980 at 437.652 kb on + strand, within QEN71_RS01980 at 437.653 kb on - strand, within QEN71_RS01980 at 437.653 kb on - strand, within QEN71_RS01980 at 437.791 kb on + strand, within QEN71_RS01980 at 437.792 kb on - strand, within QEN71_RS01980 at 437.877 kb on + strand, within QEN71_RS01980 at 437.878 kb on - strand, within QEN71_RS01980 at 437.901 kb on + strand at 437.901 kb on + strand at 437.901 kb on + strand at 437.902 kb on - strand at 437.902 kb on - strand at 437.902 kb on - strand at 437.991 kb on + strand at 437.991 kb on + strand at 437.991 kb on + strand at 437.991 kb on + strand at 437.991 kb on + strand at 437.991 kb on + strand at 437.992 kb on - strand at 437.992 kb on - strand at 437.992 kb on - strand at 437.995 kb on - strand at 438.012 kb on + strand at 438.013 kb on - strand at 438.039 kb on + strand at 438.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Filtered_supernatant_MT80_in_LB remove 436,182 + +0.2 436,261 + +1.2 436,261 + -0.7 436,261 + +0.7 436,284 - -1.7 436,301 + +0.5 436,325 + -0.0 436,325 + -0.8 436,348 + QEN71_RS01975 0.11 -2.5 436,357 + QEN71_RS01975 0.12 +1.9 436,357 + QEN71_RS01975 0.12 -1.5 436,357 + QEN71_RS01975 0.12 -0.7 436,358 - QEN71_RS01975 0.12 +1.9 436,371 - QEN71_RS01975 0.13 +0.8 436,454 + QEN71_RS01975 0.24 -0.3 436,454 + QEN71_RS01975 0.24 +0.6 436,454 + QEN71_RS01975 0.24 -0.5 436,455 - QEN71_RS01975 0.24 -1.3 436,455 - QEN71_RS01975 0.24 -0.2 436,459 + QEN71_RS01975 0.24 -2.1 436,459 + QEN71_RS01975 0.24 +0.3 436,459 + QEN71_RS01975 0.24 +0.1 436,459 + QEN71_RS01975 0.24 -0.8 436,459 + QEN71_RS01975 0.24 +0.5 436,460 - QEN71_RS01975 0.24 -0.9 436,460 - QEN71_RS01975 0.24 -0.3 436,460 - QEN71_RS01975 0.24 +0.8 436,460 - QEN71_RS01975 0.24 +0.0 436,498 + QEN71_RS01975 0.29 +1.3 436,498 + QEN71_RS01975 0.29 -2.2 436,498 + QEN71_RS01975 0.29 -2.5 436,498 + QEN71_RS01975 0.29 -2.7 436,498 + QEN71_RS01975 0.29 -1.5 436,498 + QEN71_RS01975 0.29 -0.7 436,499 - QEN71_RS01975 0.29 +0.3 436,499 - QEN71_RS01975 0.29 -0.4 436,499 - QEN71_RS01975 0.29 -0.1 436,553 + QEN71_RS01975 0.36 +0.1 436,553 + QEN71_RS01975 0.36 -0.3 436,553 + QEN71_RS01975 0.36 -1.5 436,554 - QEN71_RS01975 0.36 -0.4 436,554 - QEN71_RS01975 0.36 -0.4 436,630 + QEN71_RS01975 0.45 -0.7 436,630 + QEN71_RS01975 0.45 -0.2 436,630 + QEN71_RS01975 0.45 -0.1 436,630 + QEN71_RS01975 0.45 -0.8 436,630 + QEN71_RS01975 0.45 -0.7 436,631 - QEN71_RS01975 0.45 -0.7 436,631 - QEN71_RS01975 0.45 +0.2 436,631 - QEN71_RS01975 0.45 -0.5 436,631 - QEN71_RS01975 0.45 -1.1 436,631 - QEN71_RS01975 0.45 -0.1 436,631 - QEN71_RS01975 0.45 -0.3 436,631 - QEN71_RS01975 0.45 +0.4 436,631 - QEN71_RS01975 0.45 -0.5 436,631 - QEN71_RS01975 0.45 +0.9 436,822 + QEN71_RS01975 0.68 -1.7 436,822 + QEN71_RS01975 0.68 +1.5 436,823 - QEN71_RS01975 0.68 -2.2 436,850 + QEN71_RS01975 0.72 -0.9 436,850 + QEN71_RS01975 0.72 -0.6 436,851 - QEN71_RS01975 0.72 -1.2 436,873 + QEN71_RS01975 0.74 -0.7 436,873 + QEN71_RS01975 0.74 -1.3 436,873 + QEN71_RS01975 0.74 -0.9 436,873 + QEN71_RS01975 0.74 -0.0 436,873 + QEN71_RS01975 0.74 -0.3 436,874 - QEN71_RS01975 0.74 +0.1 436,874 - QEN71_RS01975 0.74 -0.7 436,874 - QEN71_RS01975 0.74 +0.5 436,874 - QEN71_RS01975 0.74 -2.5 436,930 + QEN71_RS01975 0.81 -2.2 436,930 + QEN71_RS01975 0.81 -0.1 436,930 + QEN71_RS01975 0.81 -1.9 436,930 + QEN71_RS01975 0.81 -1.1 437,032 + -2.2 437,032 + -0.5 437,032 + +0.7 437,033 - -0.7 437,033 - +0.6 437,044 + +0.9 437,044 + -0.6 437,045 - +0.8 437,045 - -0.4 437,444 + QEN71_RS01980 0.40 +0.1 437,445 - QEN71_RS01980 0.40 -0.9 437,486 + QEN71_RS01980 0.45 -0.4 437,486 + QEN71_RS01980 0.45 -0.0 437,487 - QEN71_RS01980 0.45 +0.9 437,487 - QEN71_RS01980 0.45 -1.9 437,529 + QEN71_RS01980 0.50 -0.8 437,530 - QEN71_RS01980 0.50 -0.4 437,533 + QEN71_RS01980 0.50 +0.1 437,533 + QEN71_RS01980 0.50 +0.9 437,533 + QEN71_RS01980 0.50 +0.9 437,533 + QEN71_RS01980 0.50 +1.4 437,652 + QEN71_RS01980 0.63 +1.3 437,652 + QEN71_RS01980 0.63 -0.5 437,652 + QEN71_RS01980 0.63 +0.3 437,653 - QEN71_RS01980 0.63 +1.3 437,653 - QEN71_RS01980 0.63 +0.1 437,791 + QEN71_RS01980 0.79 +0.7 437,792 - QEN71_RS01980 0.79 -0.4 437,877 + QEN71_RS01980 0.88 +1.2 437,878 - QEN71_RS01980 0.88 -1.0 437,901 + -0.4 437,901 + -0.6 437,901 + -1.7 437,902 - -0.4 437,902 - +0.4 437,902 - -0.0 437,991 + +0.1 437,991 + -0.8 437,991 + -0.1 437,991 + -0.1 437,991 + +0.1 437,991 + +0.7 437,992 - -0.5 437,992 - -0.4 437,992 - -0.5 437,995 - -1.1 438,012 + -0.5 438,013 - -0.1 438,039 + -0.5 438,040 - +0.5
Or see this region's nucleotide sequence