Experiment: monoculture; Experiment C, time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_04222 and MIT1002_04223 are separated by 42 nucleotides MIT1002_04223 and MIT1002_04224 are separated by 26 nucleotides MIT1002_04224 and MIT1002_04225 overlap by 17 nucleotides
MIT1002_04222: MIT1002_04222 - Chemotaxis response regulator protein-glutamate methylesterase of group 3 operon, at 90,832 to 91,878
_04222
MIT1002_04223: MIT1002_04223 - Transcriptional regulatory protein YycF, at 91,921 to 92,292
_04223
MIT1002_04224: MIT1002_04224 - Hpt domain protein, at 92,319 to 92,687
_04224
MIT1002_04225: MIT1002_04225 - KDP operon transcriptional regulatory protein KdpE, at 92,671 to 93,651
_04225
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 90.934 kb on + strand at 90.952 kb on + strand, within MIT1002_04222 at 91.128 kb on - strand, within MIT1002_04222 at 91.135 kb on + strand, within MIT1002_04222 at 91.143 kb on - strand, within MIT1002_04222 at 91.143 kb on - strand, within MIT1002_04222 at 91.202 kb on + strand, within MIT1002_04222 at 91.209 kb on - strand, within MIT1002_04222 at 91.213 kb on + strand, within MIT1002_04222 at 91.220 kb on - strand, within MIT1002_04222 at 91.228 kb on + strand, within MIT1002_04222 at 91.228 kb on + strand, within MIT1002_04222 at 91.236 kb on - strand, within MIT1002_04222 at 91.236 kb on - strand, within MIT1002_04222 at 91.236 kb on - strand, within MIT1002_04222 at 91.236 kb on - strand, within MIT1002_04222 at 91.341 kb on + strand, within MIT1002_04222 at 91.342 kb on + strand, within MIT1002_04222 at 91.349 kb on - strand, within MIT1002_04222 at 91.349 kb on - strand, within MIT1002_04222 at 91.349 kb on - strand, within MIT1002_04222 at 91.349 kb on - strand, within MIT1002_04222 at 91.350 kb on - strand, within MIT1002_04222 at 91.413 kb on - strand, within MIT1002_04222 at 91.413 kb on - strand, within MIT1002_04222 at 91.413 kb on - strand, within MIT1002_04222 at 91.485 kb on + strand, within MIT1002_04222 at 91.500 kb on + strand, within MIT1002_04222 at 91.553 kb on + strand, within MIT1002_04222 at 91.623 kb on + strand, within MIT1002_04222 at 91.631 kb on - strand, within MIT1002_04222 at 91.631 kb on - strand, within MIT1002_04222 at 91.638 kb on - strand, within MIT1002_04222 at 91.642 kb on + strand, within MIT1002_04222 at 91.664 kb on + strand, within MIT1002_04222 at 91.671 kb on + strand, within MIT1002_04222 at 91.672 kb on - strand, within MIT1002_04222 at 91.694 kb on + strand, within MIT1002_04222 at 91.747 kb on + strand, within MIT1002_04222 at 91.776 kb on + strand at 91.776 kb on + strand at 91.776 kb on + strand at 91.776 kb on + strand at 91.784 kb on - strand at 91.817 kb on - strand at 91.922 kb on - strand at 92.057 kb on + strand, within MIT1002_04223 at 92.131 kb on + strand, within MIT1002_04223 at 92.193 kb on + strand, within MIT1002_04223 at 92.201 kb on - strand, within MIT1002_04223 at 92.309 kb on + strand at 92.311 kb on + strand at 92.319 kb on + strand at 92.321 kb on + strand at 92.377 kb on - strand, within MIT1002_04224 at 92.382 kb on + strand, within MIT1002_04224 at 92.402 kb on - strand, within MIT1002_04224 at 92.406 kb on + strand, within MIT1002_04224 at 92.416 kb on + strand, within MIT1002_04224 at 92.419 kb on + strand, within MIT1002_04224 at 92.424 kb on + strand, within MIT1002_04224 at 92.424 kb on - strand, within MIT1002_04224 at 92.435 kb on + strand, within MIT1002_04224 at 92.496 kb on + strand, within MIT1002_04224 at 92.504 kb on - strand, within MIT1002_04224 at 92.506 kb on - strand, within MIT1002_04224 at 92.506 kb on - strand, within MIT1002_04224 at 92.508 kb on + strand, within MIT1002_04224 at 92.516 kb on - strand, within MIT1002_04224 at 92.516 kb on - strand, within MIT1002_04224 at 92.538 kb on + strand, within MIT1002_04224 at 92.546 kb on - strand, within MIT1002_04224 at 92.595 kb on + strand, within MIT1002_04224 at 92.596 kb on - strand, within MIT1002_04224 at 92.608 kb on - strand, within MIT1002_04224 at 92.608 kb on - strand, within MIT1002_04224 at 92.613 kb on - strand, within MIT1002_04224 at 92.617 kb on + strand, within MIT1002_04224 at 92.618 kb on + strand, within MIT1002_04224 at 92.619 kb on - strand, within MIT1002_04224 at 92.625 kb on - strand, within MIT1002_04224 at 92.625 kb on - strand, within MIT1002_04224 at 92.626 kb on - strand, within MIT1002_04224 at 92.654 kb on + strand at 92.662 kb on + strand at 92.663 kb on - strand at 92.672 kb on + strand at 92.680 kb on - strand at 92.680 kb on - strand at 92.680 kb on - strand at 92.713 kb on + strand at 92.716 kb on - strand at 92.730 kb on + strand at 92.771 kb on - strand, within MIT1002_04225 at 92.844 kb on + strand, within MIT1002_04225 at 92.844 kb on + strand, within MIT1002_04225 at 92.852 kb on - strand, within MIT1002_04225 at 92.852 kb on - strand, within MIT1002_04225 at 92.861 kb on + strand, within MIT1002_04225 at 92.871 kb on + strand, within MIT1002_04225 at 92.871 kb on + strand, within MIT1002_04225 at 92.879 kb on - strand, within MIT1002_04225 at 92.896 kb on - strand, within MIT1002_04225 at 92.896 kb on - strand, within MIT1002_04225 at 92.982 kb on - strand, within MIT1002_04225 at 93.081 kb on + strand, within MIT1002_04225 at 93.114 kb on - strand, within MIT1002_04225 at 93.114 kb on - strand, within MIT1002_04225 at 93.160 kb on + strand, within MIT1002_04225 at 93.160 kb on + strand, within MIT1002_04225 at 93.168 kb on - strand, within MIT1002_04225 at 93.168 kb on - strand, within MIT1002_04225 at 93.246 kb on + strand, within MIT1002_04225 at 93.246 kb on + strand, within MIT1002_04225 at 93.246 kb on + strand, within MIT1002_04225 at 93.246 kb on + strand, within MIT1002_04225 at 93.246 kb on + strand, within MIT1002_04225 at 93.250 kb on + strand, within MIT1002_04225 at 93.254 kb on - strand, within MIT1002_04225 at 93.254 kb on - strand, within MIT1002_04225 at 93.254 kb on - strand, within MIT1002_04225 at 93.254 kb on - strand, within MIT1002_04225 at 93.254 kb on - strand, within MIT1002_04225 at 93.254 kb on - strand, within MIT1002_04225 at 93.254 kb on - strand, within MIT1002_04225 at 93.258 kb on - strand, within MIT1002_04225 at 93.259 kb on - strand, within MIT1002_04225 at 93.266 kb on - strand, within MIT1002_04225 at 93.268 kb on - strand, within MIT1002_04225 at 93.275 kb on + strand, within MIT1002_04225
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 1 remove 90,934 + +0.2 90,952 + MIT1002_04222 0.11 +0.4 91,128 - MIT1002_04222 0.28 -0.2 91,135 + MIT1002_04222 0.29 -2.6 91,143 - MIT1002_04222 0.30 +0.6 91,143 - MIT1002_04222 0.30 +1.4 91,202 + MIT1002_04222 0.35 +0.3 91,209 - MIT1002_04222 0.36 +0.6 91,213 + MIT1002_04222 0.36 -0.1 91,220 - MIT1002_04222 0.37 +1.6 91,228 + MIT1002_04222 0.38 +0.5 91,228 + MIT1002_04222 0.38 -0.5 91,236 - MIT1002_04222 0.39 +0.4 91,236 - MIT1002_04222 0.39 -2.3 91,236 - MIT1002_04222 0.39 -0.2 91,236 - MIT1002_04222 0.39 -1.2 91,341 + MIT1002_04222 0.49 +1.2 91,342 + MIT1002_04222 0.49 -0.5 91,349 - MIT1002_04222 0.49 -0.2 91,349 - MIT1002_04222 0.49 +1.1 91,349 - MIT1002_04222 0.49 +0.1 91,349 - MIT1002_04222 0.49 -0.5 91,350 - MIT1002_04222 0.49 +0.9 91,413 - MIT1002_04222 0.55 -0.0 91,413 - MIT1002_04222 0.55 +1.7 91,413 - MIT1002_04222 0.55 +0.5 91,485 + MIT1002_04222 0.62 +0.3 91,500 + MIT1002_04222 0.64 +0.1 91,553 + MIT1002_04222 0.69 +0.2 91,623 + MIT1002_04222 0.76 -0.6 91,631 - MIT1002_04222 0.76 -1.2 91,631 - MIT1002_04222 0.76 -0.6 91,638 - MIT1002_04222 0.77 +0.2 91,642 + MIT1002_04222 0.77 +0.9 91,664 + MIT1002_04222 0.79 -1.1 91,671 + MIT1002_04222 0.80 +0.7 91,672 - MIT1002_04222 0.80 +0.7 91,694 + MIT1002_04222 0.82 -0.4 91,747 + MIT1002_04222 0.87 -0.2 91,776 + +0.8 91,776 + -0.4 91,776 + +0.7 91,776 + +2.0 91,784 - -0.0 91,817 - +0.7 91,922 - -0.4 92,057 + MIT1002_04223 0.37 +1.1 92,131 + MIT1002_04223 0.56 +1.1 92,193 + MIT1002_04223 0.73 -0.3 92,201 - MIT1002_04223 0.75 +0.7 92,309 + -0.7 92,311 + -0.1 92,319 + -1.0 92,321 + -0.8 92,377 - MIT1002_04224 0.16 +0.1 92,382 + MIT1002_04224 0.17 -0.2 92,402 - MIT1002_04224 0.22 -0.6 92,406 + MIT1002_04224 0.24 -0.4 92,416 + MIT1002_04224 0.26 +0.2 92,419 + MIT1002_04224 0.27 -0.9 92,424 + MIT1002_04224 0.28 -0.1 92,424 - MIT1002_04224 0.28 +1.1 92,435 + MIT1002_04224 0.31 -0.9 92,496 + MIT1002_04224 0.48 -0.2 92,504 - MIT1002_04224 0.50 +1.5 92,506 - MIT1002_04224 0.51 +0.2 92,506 - MIT1002_04224 0.51 -1.7 92,508 + MIT1002_04224 0.51 +2.0 92,516 - MIT1002_04224 0.53 -1.3 92,516 - MIT1002_04224 0.53 -0.6 92,538 + MIT1002_04224 0.59 -1.2 92,546 - MIT1002_04224 0.62 +0.1 92,595 + MIT1002_04224 0.75 +0.3 92,596 - MIT1002_04224 0.75 -0.6 92,608 - MIT1002_04224 0.78 +1.1 92,608 - MIT1002_04224 0.78 +0.3 92,613 - MIT1002_04224 0.80 -0.4 92,617 + MIT1002_04224 0.81 -0.4 92,618 + MIT1002_04224 0.81 +0.7 92,619 - MIT1002_04224 0.81 -1.3 92,625 - MIT1002_04224 0.83 -0.7 92,625 - MIT1002_04224 0.83 -0.3 92,626 - MIT1002_04224 0.83 -1.0 92,654 + +0.2 92,662 + +0.0 92,663 - +3.5 92,672 + -0.3 92,680 - +1.3 92,680 - +0.8 92,680 - -0.2 92,713 + +0.2 92,716 - +0.0 92,730 + +0.5 92,771 - MIT1002_04225 0.10 +0.8 92,844 + MIT1002_04225 0.18 +0.2 92,844 + MIT1002_04225 0.18 -0.1 92,852 - MIT1002_04225 0.18 -0.0 92,852 - MIT1002_04225 0.18 +0.7 92,861 + MIT1002_04225 0.19 -2.9 92,871 + MIT1002_04225 0.20 -3.3 92,871 + MIT1002_04225 0.20 +0.1 92,879 - MIT1002_04225 0.21 +0.6 92,896 - MIT1002_04225 0.23 +0.1 92,896 - MIT1002_04225 0.23 -1.7 92,982 - MIT1002_04225 0.32 -0.2 93,081 + MIT1002_04225 0.42 -0.2 93,114 - MIT1002_04225 0.45 -1.1 93,114 - MIT1002_04225 0.45 +1.2 93,160 + MIT1002_04225 0.50 -0.3 93,160 + MIT1002_04225 0.50 -1.2 93,168 - MIT1002_04225 0.51 -0.2 93,168 - MIT1002_04225 0.51 +0.1 93,246 + MIT1002_04225 0.59 -0.0 93,246 + MIT1002_04225 0.59 -0.1 93,246 + MIT1002_04225 0.59 +2.2 93,246 + MIT1002_04225 0.59 +0.6 93,246 + MIT1002_04225 0.59 -0.9 93,250 + MIT1002_04225 0.59 -0.0 93,254 - MIT1002_04225 0.59 -0.2 93,254 - MIT1002_04225 0.59 -0.3 93,254 - MIT1002_04225 0.59 -0.6 93,254 - MIT1002_04225 0.59 -0.1 93,254 - MIT1002_04225 0.59 -0.5 93,254 - MIT1002_04225 0.59 -0.3 93,254 - MIT1002_04225 0.59 -0.5 93,258 - MIT1002_04225 0.60 +1.2 93,259 - MIT1002_04225 0.60 -0.0 93,266 - MIT1002_04225 0.61 -2.5 93,268 - MIT1002_04225 0.61 +6.6 93,275 + MIT1002_04225 0.62 -0.8
Or see this region's nucleotide sequence