Experiment: Filtered_supernatant_MT71_in_LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS42175 and QEN71_RS42180 are separated by 181 nucleotides QEN71_RS42180 and QEN71_RS42185 overlap by 10 nucleotides
QEN71_RS42175: QEN71_RS42175 - nif-specific transcriptional activator NifA, at 517,576 to 519,219
_RS42175
QEN71_RS42180: QEN71_RS42180 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 519,401 to 520,933
_RS42180
QEN71_RS42185: QEN71_RS42185 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 520,924 to 522,273
_RS42185
Position (kb) 519
520
521 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 518.447 kb on + strand, within QEN71_RS42175 at 518.488 kb on - strand, within QEN71_RS42175 at 518.549 kb on + strand, within QEN71_RS42175 at 518.579 kb on + strand, within QEN71_RS42175 at 518.579 kb on + strand, within QEN71_RS42175 at 518.579 kb on + strand, within QEN71_RS42175 at 518.579 kb on + strand, within QEN71_RS42175 at 518.580 kb on - strand, within QEN71_RS42175 at 518.580 kb on - strand, within QEN71_RS42175 at 518.650 kb on + strand, within QEN71_RS42175 at 518.651 kb on - strand, within QEN71_RS42175 at 518.653 kb on + strand, within QEN71_RS42175 at 518.670 kb on - strand, within QEN71_RS42175 at 518.670 kb on - strand, within QEN71_RS42175 at 518.673 kb on + strand, within QEN71_RS42175 at 518.673 kb on + strand, within QEN71_RS42175 at 518.673 kb on + strand, within QEN71_RS42175 at 518.674 kb on - strand, within QEN71_RS42175 at 518.674 kb on - strand, within QEN71_RS42175 at 518.729 kb on - strand, within QEN71_RS42175 at 518.766 kb on - strand, within QEN71_RS42175 at 518.810 kb on - strand, within QEN71_RS42175 at 518.882 kb on + strand, within QEN71_RS42175 at 518.882 kb on + strand, within QEN71_RS42175 at 518.882 kb on + strand, within QEN71_RS42175 at 518.882 kb on + strand, within QEN71_RS42175 at 518.882 kb on + strand, within QEN71_RS42175 at 518.883 kb on - strand, within QEN71_RS42175 at 518.883 kb on - strand, within QEN71_RS42175 at 518.883 kb on - strand, within QEN71_RS42175 at 518.930 kb on + strand, within QEN71_RS42175 at 518.931 kb on - strand, within QEN71_RS42175 at 518.931 kb on - strand, within QEN71_RS42175 at 518.931 kb on - strand, within QEN71_RS42175 at 518.931 kb on - strand, within QEN71_RS42175 at 519.036 kb on + strand, within QEN71_RS42175 at 519.207 kb on - strand at 519.207 kb on - strand at 519.233 kb on - strand at 519.236 kb on + strand at 519.236 kb on + strand at 519.236 kb on + strand at 519.236 kb on + strand at 519.236 kb on + strand at 519.236 kb on + strand at 519.236 kb on + strand at 519.237 kb on - strand at 519.237 kb on - strand at 519.237 kb on - strand at 519.426 kb on + strand at 519.426 kb on + strand at 519.426 kb on + strand at 519.427 kb on - strand at 519.427 kb on - strand at 519.705 kb on - strand, within QEN71_RS42180 at 519.705 kb on - strand, within QEN71_RS42180 at 519.777 kb on + strand, within QEN71_RS42180 at 519.777 kb on + strand, within QEN71_RS42180 at 519.778 kb on - strand, within QEN71_RS42180 at 519.780 kb on - strand, within QEN71_RS42180 at 519.816 kb on + strand, within QEN71_RS42180 at 519.817 kb on - strand, within QEN71_RS42180 at 519.864 kb on + strand, within QEN71_RS42180 at 519.864 kb on + strand, within QEN71_RS42180 at 519.865 kb on - strand, within QEN71_RS42180 at 519.967 kb on + strand, within QEN71_RS42180 at 519.967 kb on + strand, within QEN71_RS42180 at 519.985 kb on + strand, within QEN71_RS42180 at 519.985 kb on + strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.988 kb on + strand, within QEN71_RS42180 at 519.988 kb on + strand, within QEN71_RS42180 at 519.988 kb on + strand, within QEN71_RS42180 at 520.002 kb on - strand, within QEN71_RS42180 at 520.003 kb on + strand, within QEN71_RS42180 at 520.003 kb on + strand, within QEN71_RS42180 at 520.004 kb on - strand, within QEN71_RS42180 at 520.060 kb on + strand, within QEN71_RS42180 at 520.060 kb on + strand, within QEN71_RS42180 at 520.061 kb on - strand, within QEN71_RS42180 at 520.061 kb on - strand, within QEN71_RS42180 at 520.061 kb on - strand, within QEN71_RS42180 at 520.062 kb on + strand, within QEN71_RS42180 at 520.062 kb on + strand, within QEN71_RS42180 at 520.062 kb on + strand, within QEN71_RS42180 at 520.063 kb on - strand, within QEN71_RS42180 at 520.063 kb on - strand, within QEN71_RS42180 at 520.063 kb on - strand, within QEN71_RS42180 at 520.092 kb on - strand, within QEN71_RS42180 at 520.130 kb on - strand, within QEN71_RS42180 at 520.130 kb on - strand, within QEN71_RS42180 at 520.130 kb on - strand, within QEN71_RS42180 at 520.197 kb on - strand, within QEN71_RS42180 at 520.197 kb on - strand, within QEN71_RS42180 at 520.469 kb on - strand, within QEN71_RS42180 at 520.540 kb on + strand, within QEN71_RS42180 at 520.541 kb on - strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.560 kb on - strand, within QEN71_RS42180 at 520.616 kb on + strand, within QEN71_RS42180 at 520.616 kb on + strand, within QEN71_RS42180 at 520.616 kb on + strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.756 kb on + strand, within QEN71_RS42180 at 520.756 kb on + strand, within QEN71_RS42180 at 520.757 kb on - strand, within QEN71_RS42180 at 520.931 kb on + strand at 520.999 kb on + strand at 520.999 kb on + strand at 521.133 kb on + strand, within QEN71_RS42185 at 521.133 kb on + strand, within QEN71_RS42185 at 521.133 kb on + strand, within QEN71_RS42185 at 521.134 kb on - strand, within QEN71_RS42185 at 521.134 kb on - strand, within QEN71_RS42185 at 521.139 kb on + strand, within QEN71_RS42185 at 521.178 kb on - strand, within QEN71_RS42185 at 521.178 kb on - strand, within QEN71_RS42185 at 521.188 kb on - strand, within QEN71_RS42185 at 521.318 kb on - strand, within QEN71_RS42185 at 521.336 kb on - strand, within QEN71_RS42185 at 521.336 kb on - strand, within QEN71_RS42185 at 521.336 kb on - strand, within QEN71_RS42185 at 521.410 kb on - strand, within QEN71_RS42185 at 521.410 kb on - strand, within QEN71_RS42185 at 521.414 kb on + strand, within QEN71_RS42185 at 521.415 kb on - strand, within QEN71_RS42185 at 521.450 kb on + strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.586 kb on + strand, within QEN71_RS42185 at 521.586 kb on + strand, within QEN71_RS42185 at 521.587 kb on - strand, within QEN71_RS42185 at 521.587 kb on - strand, within QEN71_RS42185 at 521.626 kb on - strand, within QEN71_RS42185 at 521.786 kb on - strand, within QEN71_RS42185 at 521.837 kb on - strand, within QEN71_RS42185
Per-strain Table
Position Strand Gene LocusTag Fraction Filtered_supernatant_MT71_in_LB remove 518,447 + QEN71_RS42175 0.53 +0.1 518,488 - QEN71_RS42175 0.55 -0.4 518,549 + QEN71_RS42175 0.59 -1.1 518,579 + QEN71_RS42175 0.61 -0.5 518,579 + QEN71_RS42175 0.61 -0.6 518,579 + QEN71_RS42175 0.61 +0.2 518,579 + QEN71_RS42175 0.61 -0.0 518,580 - QEN71_RS42175 0.61 +1.3 518,580 - QEN71_RS42175 0.61 +0.9 518,650 + QEN71_RS42175 0.65 -1.9 518,651 - QEN71_RS42175 0.65 -0.2 518,653 + QEN71_RS42175 0.66 -0.3 518,670 - QEN71_RS42175 0.67 -0.3 518,670 - QEN71_RS42175 0.67 -0.3 518,673 + QEN71_RS42175 0.67 -0.3 518,673 + QEN71_RS42175 0.67 -0.5 518,673 + QEN71_RS42175 0.67 -0.5 518,674 - QEN71_RS42175 0.67 -0.7 518,674 - QEN71_RS42175 0.67 -0.3 518,729 - QEN71_RS42175 0.70 +0.3 518,766 - QEN71_RS42175 0.72 -1.0 518,810 - QEN71_RS42175 0.75 -0.9 518,882 + QEN71_RS42175 0.79 -0.4 518,882 + QEN71_RS42175 0.79 -0.6 518,882 + QEN71_RS42175 0.79 -0.1 518,882 + QEN71_RS42175 0.79 -1.2 518,882 + QEN71_RS42175 0.79 +0.5 518,883 - QEN71_RS42175 0.80 -0.9 518,883 - QEN71_RS42175 0.80 -0.8 518,883 - QEN71_RS42175 0.80 -1.3 518,930 + QEN71_RS42175 0.82 -1.0 518,931 - QEN71_RS42175 0.82 +0.2 518,931 - QEN71_RS42175 0.82 -0.8 518,931 - QEN71_RS42175 0.82 -0.2 518,931 - QEN71_RS42175 0.82 -1.7 519,036 + QEN71_RS42175 0.89 -0.1 519,207 - -0.1 519,207 - -0.3 519,233 - -0.5 519,236 + +0.4 519,236 + -1.3 519,236 + -0.2 519,236 + +1.6 519,236 + -0.7 519,236 + -0.6 519,236 + +0.5 519,237 - +1.0 519,237 - +0.6 519,237 - +0.0 519,426 + -0.6 519,426 + +0.4 519,426 + -0.8 519,427 - -0.0 519,427 - +0.3 519,705 - QEN71_RS42180 0.20 +0.3 519,705 - QEN71_RS42180 0.20 -0.3 519,777 + QEN71_RS42180 0.25 +0.9 519,777 + QEN71_RS42180 0.25 +1.4 519,778 - QEN71_RS42180 0.25 -1.0 519,780 - QEN71_RS42180 0.25 -0.5 519,816 + QEN71_RS42180 0.27 -0.8 519,817 - QEN71_RS42180 0.27 -0.6 519,864 + QEN71_RS42180 0.30 -1.8 519,864 + QEN71_RS42180 0.30 +2.8 519,865 - QEN71_RS42180 0.30 -0.3 519,967 + QEN71_RS42180 0.37 -0.6 519,967 + QEN71_RS42180 0.37 +1.2 519,985 + QEN71_RS42180 0.38 +0.6 519,985 + QEN71_RS42180 0.38 -1.3 519,986 - QEN71_RS42180 0.38 +2.0 519,986 - QEN71_RS42180 0.38 -2.5 519,986 - QEN71_RS42180 0.38 -1.5 519,986 - QEN71_RS42180 0.38 -0.8 519,988 + QEN71_RS42180 0.38 +1.0 519,988 + QEN71_RS42180 0.38 -0.0 519,988 + QEN71_RS42180 0.38 -0.2 520,002 - QEN71_RS42180 0.39 -0.3 520,003 + QEN71_RS42180 0.39 -0.3 520,003 + QEN71_RS42180 0.39 -1.5 520,004 - QEN71_RS42180 0.39 -1.2 520,060 + QEN71_RS42180 0.43 -1.3 520,060 + QEN71_RS42180 0.43 -0.6 520,061 - QEN71_RS42180 0.43 -0.2 520,061 - QEN71_RS42180 0.43 +0.4 520,061 - QEN71_RS42180 0.43 -0.0 520,062 + QEN71_RS42180 0.43 -0.4 520,062 + QEN71_RS42180 0.43 +0.6 520,062 + QEN71_RS42180 0.43 +0.5 520,063 - QEN71_RS42180 0.43 -0.6 520,063 - QEN71_RS42180 0.43 -0.5 520,063 - QEN71_RS42180 0.43 -0.9 520,092 - QEN71_RS42180 0.45 +0.7 520,130 - QEN71_RS42180 0.48 +0.1 520,130 - QEN71_RS42180 0.48 +0.3 520,130 - QEN71_RS42180 0.48 +1.1 520,197 - QEN71_RS42180 0.52 +0.1 520,197 - QEN71_RS42180 0.52 -0.0 520,469 - QEN71_RS42180 0.70 -0.2 520,540 + QEN71_RS42180 0.74 -1.3 520,541 - QEN71_RS42180 0.74 +0.1 520,559 + QEN71_RS42180 0.76 +1.3 520,559 + QEN71_RS42180 0.76 +0.6 520,559 + QEN71_RS42180 0.76 -0.2 520,559 + QEN71_RS42180 0.76 -0.3 520,559 + QEN71_RS42180 0.76 -0.4 520,559 + QEN71_RS42180 0.76 +2.4 520,560 - QEN71_RS42180 0.76 -0.1 520,616 + QEN71_RS42180 0.79 +0.4 520,616 + QEN71_RS42180 0.79 +0.4 520,616 + QEN71_RS42180 0.79 +1.2 520,617 - QEN71_RS42180 0.79 -0.6 520,617 - QEN71_RS42180 0.79 +1.3 520,617 - QEN71_RS42180 0.79 +0.2 520,617 - QEN71_RS42180 0.79 +0.1 520,756 + QEN71_RS42180 0.88 -1.7 520,756 + QEN71_RS42180 0.88 +0.6 520,757 - QEN71_RS42180 0.88 +1.3 520,931 + -1.1 520,999 + -0.9 520,999 + +1.3 521,133 + QEN71_RS42185 0.15 -1.6 521,133 + QEN71_RS42185 0.15 +0.2 521,133 + QEN71_RS42185 0.15 +1.7 521,134 - QEN71_RS42185 0.16 -0.0 521,134 - QEN71_RS42185 0.16 -0.6 521,139 + QEN71_RS42185 0.16 -1.1 521,178 - QEN71_RS42185 0.19 -0.7 521,178 - QEN71_RS42185 0.19 -1.1 521,188 - QEN71_RS42185 0.20 -0.3 521,318 - QEN71_RS42185 0.29 -0.2 521,336 - QEN71_RS42185 0.31 -0.4 521,336 - QEN71_RS42185 0.31 -2.3 521,336 - QEN71_RS42185 0.31 +0.5 521,410 - QEN71_RS42185 0.36 -0.6 521,410 - QEN71_RS42185 0.36 -0.8 521,414 + QEN71_RS42185 0.36 -2.4 521,415 - QEN71_RS42185 0.36 +1.5 521,450 + QEN71_RS42185 0.39 -0.9 521,451 - QEN71_RS42185 0.39 -1.4 521,451 - QEN71_RS42185 0.39 -0.5 521,451 - QEN71_RS42185 0.39 -0.5 521,451 - QEN71_RS42185 0.39 -0.5 521,586 + QEN71_RS42185 0.49 +0.6 521,586 + QEN71_RS42185 0.49 -0.1 521,587 - QEN71_RS42185 0.49 -0.2 521,587 - QEN71_RS42185 0.49 -0.1 521,626 - QEN71_RS42185 0.52 -0.0 521,786 - QEN71_RS42185 0.64 +1.7 521,837 - QEN71_RS42185 0.68 -0.0
Or see this region's nucleotide sequence