Experiment: monoculture; Experiment B, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03330 and MIT1002_03331 are separated by 122 nucleotides MIT1002_03331 and MIT1002_03332 are separated by 274 nucleotides MIT1002_03332 and MIT1002_03333 are separated by 35 nucleotides
MIT1002_03330: MIT1002_03330 - L-lactate dehydrogenase [cytochrome], at 3,720,507 to 3,721,640
_03330
MIT1002_03331: MIT1002_03331 - D-lactate dehydrogenase, at 3,721,763 to 3,723,496
_03331
MIT1002_03332: MIT1002_03332 - CsgBAC operon transcriptional regulatory protein, at 3,723,771 to 3,724,448
_03332
MIT1002_03333: MIT1002_03333 - curli production assembly/transport protein CsgG, at 3,724,484 to 3,725,245
_03333
Position (kb)
3721
3722
3723
3724 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 3720.818 kb on - strand, within MIT1002_03330 at 3720.836 kb on - strand, within MIT1002_03330 at 3720.888 kb on - strand, within MIT1002_03330 at 3720.935 kb on + strand, within MIT1002_03330 at 3720.943 kb on - strand, within MIT1002_03330 at 3720.943 kb on - strand, within MIT1002_03330 at 3720.949 kb on + strand, within MIT1002_03330 at 3720.965 kb on - strand, within MIT1002_03330 at 3721.017 kb on + strand, within MIT1002_03330 at 3721.025 kb on - strand, within MIT1002_03330 at 3721.213 kb on + strand, within MIT1002_03330 at 3721.220 kb on - strand, within MIT1002_03330 at 3721.222 kb on + strand, within MIT1002_03330 at 3721.243 kb on + strand, within MIT1002_03330 at 3721.320 kb on - strand, within MIT1002_03330 at 3721.353 kb on + strand, within MIT1002_03330 at 3721.366 kb on - strand, within MIT1002_03330 at 3721.448 kb on - strand, within MIT1002_03330 at 3721.518 kb on + strand, within MIT1002_03330 at 3721.546 kb on + strand at 3721.549 kb on - strand at 3721.592 kb on - strand at 3721.592 kb on - strand at 3721.776 kb on + strand at 3721.785 kb on + strand at 3721.853 kb on - strand at 3721.856 kb on + strand at 3721.856 kb on + strand at 3721.864 kb on - strand at 3721.864 kb on - strand at 3721.898 kb on + strand at 3721.945 kb on + strand, within MIT1002_03331 at 3721.953 kb on - strand, within MIT1002_03331 at 3722.058 kb on - strand, within MIT1002_03331 at 3722.097 kb on - strand, within MIT1002_03331 at 3722.171 kb on + strand, within MIT1002_03331 at 3722.402 kb on - strand, within MIT1002_03331 at 3722.412 kb on - strand, within MIT1002_03331 at 3722.584 kb on - strand, within MIT1002_03331 at 3722.621 kb on + strand, within MIT1002_03331 at 3722.633 kb on + strand, within MIT1002_03331 at 3722.719 kb on + strand, within MIT1002_03331 at 3722.738 kb on + strand, within MIT1002_03331 at 3722.738 kb on + strand, within MIT1002_03331 at 3722.755 kb on + strand, within MIT1002_03331 at 3722.784 kb on + strand, within MIT1002_03331 at 3722.787 kb on - strand, within MIT1002_03331 at 3723.074 kb on + strand, within MIT1002_03331 at 3723.121 kb on - strand, within MIT1002_03331 at 3723.232 kb on - strand, within MIT1002_03331 at 3723.237 kb on - strand, within MIT1002_03331 at 3723.244 kb on + strand, within MIT1002_03331 at 3723.262 kb on + strand, within MIT1002_03331 at 3723.289 kb on - strand, within MIT1002_03331 at 3723.396 kb on + strand at 3723.396 kb on + strand at 3723.396 kb on + strand at 3723.396 kb on + strand at 3723.465 kb on + strand at 3723.485 kb on - strand at 3723.492 kb on - strand at 3723.537 kb on + strand at 3723.545 kb on - strand at 3723.545 kb on - strand at 3723.625 kb on + strand at 3723.700 kb on + strand at 3723.703 kb on - strand at 3723.703 kb on - strand at 3723.708 kb on - strand at 3723.708 kb on - strand at 3723.710 kb on + strand at 3723.817 kb on + strand at 3723.835 kb on - strand at 3723.904 kb on + strand, within MIT1002_03332 at 3723.925 kb on + strand, within MIT1002_03332 at 3723.931 kb on - strand, within MIT1002_03332 at 3723.931 kb on - strand, within MIT1002_03332 at 3723.933 kb on - strand, within MIT1002_03332 at 3723.958 kb on - strand, within MIT1002_03332 at 3723.959 kb on - strand, within MIT1002_03332 at 3724.007 kb on + strand, within MIT1002_03332 at 3724.034 kb on - strand, within MIT1002_03332 at 3724.059 kb on + strand, within MIT1002_03332 at 3724.081 kb on + strand, within MIT1002_03332 at 3724.110 kb on + strand, within MIT1002_03332 at 3724.142 kb on - strand, within MIT1002_03332 at 3724.199 kb on + strand, within MIT1002_03332 at 3724.232 kb on + strand, within MIT1002_03332 at 3724.250 kb on - strand, within MIT1002_03332 at 3724.255 kb on - strand, within MIT1002_03332 at 3724.315 kb on + strand, within MIT1002_03332 at 3724.396 kb on - strand at 3724.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 6 remove 3,720,818 - MIT1002_03330 0.27 -2.4 3,720,836 - MIT1002_03330 0.29 -3.2 3,720,888 - MIT1002_03330 0.34 +0.3 3,720,935 + MIT1002_03330 0.38 -0.4 3,720,943 - MIT1002_03330 0.38 -1.3 3,720,943 - MIT1002_03330 0.38 -0.2 3,720,949 + MIT1002_03330 0.39 -0.8 3,720,965 - MIT1002_03330 0.40 -0.8 3,721,017 + MIT1002_03330 0.45 -2.8 3,721,025 - MIT1002_03330 0.46 -0.5 3,721,213 + MIT1002_03330 0.62 +0.5 3,721,220 - MIT1002_03330 0.63 -2.5 3,721,222 + MIT1002_03330 0.63 -1.1 3,721,243 + MIT1002_03330 0.65 -2.5 3,721,320 - MIT1002_03330 0.72 -0.4 3,721,353 + MIT1002_03330 0.75 -2.1 3,721,366 - MIT1002_03330 0.76 -1.5 3,721,448 - MIT1002_03330 0.83 -2.7 3,721,518 + MIT1002_03330 0.89 -2.6 3,721,546 + -1.6 3,721,549 - +0.3 3,721,592 - -0.6 3,721,592 - -0.4 3,721,776 + -1.5 3,721,785 + +0.5 3,721,853 - -1.3 3,721,856 + -3.6 3,721,856 + -2.3 3,721,864 - -1.3 3,721,864 - -1.3 3,721,898 + -0.7 3,721,945 + MIT1002_03331 0.10 +0.1 3,721,953 - MIT1002_03331 0.11 +0.8 3,722,058 - MIT1002_03331 0.17 -4.1 3,722,097 - MIT1002_03331 0.19 -3.0 3,722,171 + MIT1002_03331 0.24 +0.6 3,722,402 - MIT1002_03331 0.37 -2.6 3,722,412 - MIT1002_03331 0.37 -2.1 3,722,584 - MIT1002_03331 0.47 -1.1 3,722,621 + MIT1002_03331 0.49 -2.2 3,722,633 + MIT1002_03331 0.50 -0.7 3,722,719 + MIT1002_03331 0.55 -1.2 3,722,738 + MIT1002_03331 0.56 -1.3 3,722,738 + MIT1002_03331 0.56 +0.3 3,722,755 + MIT1002_03331 0.57 +0.1 3,722,784 + MIT1002_03331 0.59 -2.0 3,722,787 - MIT1002_03331 0.59 -1.8 3,723,074 + MIT1002_03331 0.76 -0.8 3,723,121 - MIT1002_03331 0.78 -1.2 3,723,232 - MIT1002_03331 0.85 -1.2 3,723,237 - MIT1002_03331 0.85 -0.9 3,723,244 + MIT1002_03331 0.85 -3.8 3,723,262 + MIT1002_03331 0.86 +0.8 3,723,289 - MIT1002_03331 0.88 -1.7 3,723,396 + -4.3 3,723,396 + -2.1 3,723,396 + -1.4 3,723,396 + -2.0 3,723,465 + -1.4 3,723,485 - -3.7 3,723,492 - +0.8 3,723,537 + -2.2 3,723,545 - -1.0 3,723,545 - -1.0 3,723,625 + -1.2 3,723,700 + +3.5 3,723,703 - +3.4 3,723,703 - +2.2 3,723,708 - +2.9 3,723,708 - +4.0 3,723,710 + +6.8 3,723,817 + +7.2 3,723,835 - +6.2 3,723,904 + MIT1002_03332 0.20 +7.2 3,723,925 + MIT1002_03332 0.23 +9.2 3,723,931 - MIT1002_03332 0.24 +7.6 3,723,931 - MIT1002_03332 0.24 +8.1 3,723,933 - MIT1002_03332 0.24 +7.6 3,723,958 - MIT1002_03332 0.28 +7.9 3,723,959 - MIT1002_03332 0.28 +6.9 3,724,007 + MIT1002_03332 0.35 +5.2 3,724,034 - MIT1002_03332 0.39 +7.6 3,724,059 + MIT1002_03332 0.42 +7.4 3,724,081 + MIT1002_03332 0.46 +7.6 3,724,110 + MIT1002_03332 0.50 +5.8 3,724,142 - MIT1002_03332 0.55 +8.4 3,724,199 + MIT1002_03332 0.63 +8.0 3,724,232 + MIT1002_03332 0.68 +4.8 3,724,250 - MIT1002_03332 0.71 +7.1 3,724,255 - MIT1002_03332 0.71 +7.0 3,724,315 + MIT1002_03332 0.80 +6.7 3,724,396 - +7.8 3,724,450 - +0.2
Or see this region's nucleotide sequence