Experiment: monoculture; Experiment B, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01029 and MIT1002_01030 are separated by 161 nucleotides MIT1002_01030 and MIT1002_01031 are separated by 132 nucleotides MIT1002_01031 and MIT1002_01032 are separated by 485 nucleotides MIT1002_01032 and MIT1002_01033 are separated by 300 nucleotides MIT1002_01033 and MIT1002_01034 are separated by 145 nucleotides
MIT1002_01029: MIT1002_01029 - putative DNA endonuclease SmrA, at 1,142,423 to 1,143,010
_01029
MIT1002_01030: MIT1002_01030 - hypothetical protein, at 1,143,172 to 1,143,297
_01030
MIT1002_01031: MIT1002_01031 - tRNA-Arg, at 1,143,430 to 1,143,504
_01031
MIT1002_01032: MIT1002_01032 - hypothetical protein, at 1,143,990 to 1,145,171
_01032
MIT1002_01033: MIT1002_01033 - hypothetical protein, at 1,145,472 to 1,145,957
_01033
MIT1002_01034: MIT1002_01034 - FlgN protein, at 1,146,103 to 1,146,534
_01034
Position (kb)
1143
1144
1145
1146 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1143.009 kb on + strand at 1143.046 kb on - strand at 1143.066 kb on + strand at 1143.074 kb on - strand at 1143.074 kb on - strand at 1143.074 kb on - strand at 1143.076 kb on + strand at 1143.082 kb on - strand at 1143.082 kb on - strand at 1143.097 kb on - strand at 1143.097 kb on - strand at 1143.201 kb on + strand, within MIT1002_01030 at 1143.207 kb on - strand, within MIT1002_01030 at 1143.209 kb on - strand, within MIT1002_01030 at 1143.327 kb on - strand at 1143.465 kb on - strand, within MIT1002_01031 at 1143.466 kb on - strand, within MIT1002_01031 at 1143.466 kb on - strand, within MIT1002_01031 at 1143.471 kb on - strand, within MIT1002_01031 at 1143.475 kb on + strand, within MIT1002_01031 at 1143.483 kb on - strand, within MIT1002_01031 at 1143.502 kb on + strand at 1143.575 kb on - strand at 1143.625 kb on - strand at 1143.735 kb on + strand at 1143.942 kb on - strand at 1144.062 kb on - strand at 1144.105 kb on + strand at 1144.282 kb on - strand, within MIT1002_01032 at 1144.407 kb on - strand, within MIT1002_01032 at 1144.430 kb on - strand, within MIT1002_01032 at 1144.507 kb on + strand, within MIT1002_01032 at 1144.540 kb on + strand, within MIT1002_01032 at 1144.559 kb on + strand, within MIT1002_01032 at 1144.569 kb on - strand, within MIT1002_01032 at 1144.669 kb on + strand, within MIT1002_01032 at 1144.683 kb on - strand, within MIT1002_01032 at 1144.717 kb on + strand, within MIT1002_01032 at 1144.717 kb on + strand, within MIT1002_01032 at 1144.717 kb on + strand, within MIT1002_01032 at 1144.717 kb on + strand, within MIT1002_01032 at 1144.725 kb on - strand, within MIT1002_01032 at 1144.725 kb on - strand, within MIT1002_01032 at 1144.725 kb on - strand, within MIT1002_01032 at 1144.725 kb on - strand, within MIT1002_01032 at 1144.727 kb on + strand, within MIT1002_01032 at 1144.734 kb on - strand, within MIT1002_01032 at 1144.741 kb on + strand, within MIT1002_01032 at 1144.749 kb on - strand, within MIT1002_01032 at 1144.749 kb on - strand, within MIT1002_01032 at 1144.758 kb on - strand, within MIT1002_01032 at 1144.758 kb on - strand, within MIT1002_01032 at 1144.881 kb on - strand, within MIT1002_01032 at 1144.998 kb on + strand, within MIT1002_01032 at 1145.057 kb on - strand at 1145.126 kb on - strand at 1145.128 kb on - strand at 1145.179 kb on + strand at 1145.201 kb on + strand at 1145.206 kb on + strand at 1145.322 kb on + strand at 1145.322 kb on + strand at 1145.354 kb on - strand at 1145.370 kb on + strand at 1145.395 kb on + strand at 1145.420 kb on - strand at 1145.616 kb on + strand, within MIT1002_01033 at 1145.624 kb on - strand, within MIT1002_01033 at 1145.707 kb on + strand, within MIT1002_01033 at 1145.764 kb on - strand, within MIT1002_01033 at 1145.772 kb on + strand, within MIT1002_01033 at 1145.780 kb on - strand, within MIT1002_01033 at 1145.780 kb on - strand, within MIT1002_01033 at 1145.780 kb on - strand, within MIT1002_01033 at 1145.797 kb on + strand, within MIT1002_01033 at 1145.800 kb on + strand, within MIT1002_01033 at 1145.808 kb on - strand, within MIT1002_01033 at 1145.810 kb on - strand, within MIT1002_01033 at 1145.910 kb on - strand at 1146.001 kb on + strand at 1146.052 kb on + strand at 1146.053 kb on + strand at 1146.062 kb on + strand at 1146.083 kb on - strand at 1146.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 6 remove 1,143,009 + +0.5 1,143,046 - +2.1 1,143,066 + +1.0 1,143,074 - -2.0 1,143,074 - -0.1 1,143,074 - +2.1 1,143,076 + -0.2 1,143,082 - -0.1 1,143,082 - +1.1 1,143,097 - +0.9 1,143,097 - -1.2 1,143,201 + MIT1002_01030 0.23 -1.2 1,143,207 - MIT1002_01030 0.28 +0.1 1,143,209 - MIT1002_01030 0.29 +0.8 1,143,327 - +0.9 1,143,465 - MIT1002_01031 0.47 +0.5 1,143,466 - MIT1002_01031 0.48 -0.3 1,143,466 - MIT1002_01031 0.48 +0.3 1,143,471 - MIT1002_01031 0.55 +1.9 1,143,475 + MIT1002_01031 0.60 +0.5 1,143,483 - MIT1002_01031 0.71 +1.7 1,143,502 + -0.3 1,143,575 - +1.3 1,143,625 - +0.3 1,143,735 + +1.0 1,143,942 - +1.9 1,144,062 - -2.3 1,144,105 + +0.8 1,144,282 - MIT1002_01032 0.25 +2.1 1,144,407 - MIT1002_01032 0.35 +0.6 1,144,430 - MIT1002_01032 0.37 +1.3 1,144,507 + MIT1002_01032 0.44 -0.2 1,144,540 + MIT1002_01032 0.47 +0.7 1,144,559 + MIT1002_01032 0.48 -0.6 1,144,569 - MIT1002_01032 0.49 -0.4 1,144,669 + MIT1002_01032 0.57 -0.1 1,144,683 - MIT1002_01032 0.59 -0.4 1,144,717 + MIT1002_01032 0.62 -0.4 1,144,717 + MIT1002_01032 0.62 +2.7 1,144,717 + MIT1002_01032 0.62 +3.6 1,144,717 + MIT1002_01032 0.62 +0.2 1,144,725 - MIT1002_01032 0.62 -0.3 1,144,725 - MIT1002_01032 0.62 -0.5 1,144,725 - MIT1002_01032 0.62 -1.0 1,144,725 - MIT1002_01032 0.62 -1.4 1,144,727 + MIT1002_01032 0.62 +0.8 1,144,734 - MIT1002_01032 0.63 +1.1 1,144,741 + MIT1002_01032 0.64 +1.2 1,144,749 - MIT1002_01032 0.64 -0.6 1,144,749 - MIT1002_01032 0.64 +0.8 1,144,758 - MIT1002_01032 0.65 +0.8 1,144,758 - MIT1002_01032 0.65 -0.5 1,144,881 - MIT1002_01032 0.75 +0.1 1,144,998 + MIT1002_01032 0.85 +0.6 1,145,057 - +2.9 1,145,126 - +0.8 1,145,128 - -0.1 1,145,179 + +0.3 1,145,201 + -1.0 1,145,206 + -0.4 1,145,322 + +0.3 1,145,322 + +1.8 1,145,354 - -1.7 1,145,370 + -0.4 1,145,395 + -1.7 1,145,420 - -1.4 1,145,616 + MIT1002_01033 0.30 +0.4 1,145,624 - MIT1002_01033 0.31 -0.1 1,145,707 + MIT1002_01033 0.48 -1.0 1,145,764 - MIT1002_01033 0.60 +1.9 1,145,772 + MIT1002_01033 0.62 -0.6 1,145,780 - MIT1002_01033 0.63 -1.0 1,145,780 - MIT1002_01033 0.63 +0.1 1,145,780 - MIT1002_01033 0.63 -1.4 1,145,797 + MIT1002_01033 0.67 +0.5 1,145,800 + MIT1002_01033 0.67 -0.8 1,145,808 - MIT1002_01033 0.69 +0.7 1,145,810 - MIT1002_01033 0.70 +2.8 1,145,910 - +0.2 1,146,001 + +0.8 1,146,052 + +1.0 1,146,053 + -0.9 1,146,062 + +0.1 1,146,083 - -1.8 1,146,083 - -0.4
Or see this region's nucleotide sequence