Experiment: monoculture; Experiment B, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00419 and MIT1002_00420 are separated by 183 nucleotides MIT1002_00420 and MIT1002_00421 are separated by 28 nucleotides
MIT1002_00419: MIT1002_00419 - Putative gamma-glutamyltransferase YwrD, at 452,378 to 454,069
_00419
MIT1002_00420: MIT1002_00420 - putative deferrochelatase/peroxidase YfeX, at 454,253 to 455,179
_00420
MIT1002_00421: MIT1002_00421 - Adenylate cyclase 1, at 455,208 to 456,281
_00421
Position (kb)
454
455
456 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 453.268 kb on + strand, within MIT1002_00419 at 453.287 kb on + strand, within MIT1002_00419 at 453.287 kb on + strand, within MIT1002_00419 at 453.292 kb on + strand, within MIT1002_00419 at 453.295 kb on - strand, within MIT1002_00419 at 453.316 kb on - strand, within MIT1002_00419 at 453.316 kb on - strand, within MIT1002_00419 at 453.358 kb on + strand, within MIT1002_00419 at 453.490 kb on + strand, within MIT1002_00419 at 453.525 kb on + strand, within MIT1002_00419 at 453.574 kb on - strand, within MIT1002_00419 at 453.580 kb on - strand, within MIT1002_00419 at 453.709 kb on + strand, within MIT1002_00419 at 453.719 kb on + strand, within MIT1002_00419 at 453.824 kb on + strand, within MIT1002_00419 at 453.832 kb on - strand, within MIT1002_00419 at 453.832 kb on - strand, within MIT1002_00419 at 453.845 kb on + strand, within MIT1002_00419 at 453.853 kb on - strand, within MIT1002_00419 at 453.858 kb on - strand, within MIT1002_00419 at 454.006 kb on + strand at 454.006 kb on + strand at 454.014 kb on - strand at 454.065 kb on + strand at 454.076 kb on - strand at 454.097 kb on + strand at 454.144 kb on + strand at 454.144 kb on - strand at 454.217 kb on + strand at 454.225 kb on - strand at 454.231 kb on + strand at 454.247 kb on - strand at 454.273 kb on + strand at 454.281 kb on - strand at 454.281 kb on - strand at 454.303 kb on + strand at 454.303 kb on + strand at 454.453 kb on + strand, within MIT1002_00420 at 454.459 kb on - strand, within MIT1002_00420 at 454.590 kb on + strand, within MIT1002_00420 at 454.647 kb on + strand, within MIT1002_00420 at 454.647 kb on + strand, within MIT1002_00420 at 454.665 kb on + strand, within MIT1002_00420 at 454.665 kb on + strand, within MIT1002_00420 at 454.673 kb on - strand, within MIT1002_00420 at 454.680 kb on - strand, within MIT1002_00420 at 454.778 kb on - strand, within MIT1002_00420 at 454.799 kb on + strand, within MIT1002_00420 at 454.807 kb on - strand, within MIT1002_00420 at 454.815 kb on - strand, within MIT1002_00420 at 454.905 kb on + strand, within MIT1002_00420 at 454.912 kb on + strand, within MIT1002_00420 at 454.920 kb on - strand, within MIT1002_00420 at 454.955 kb on + strand, within MIT1002_00420 at 454.982 kb on - strand, within MIT1002_00420 at 454.999 kb on - strand, within MIT1002_00420 at 455.028 kb on + strand, within MIT1002_00420 at 455.028 kb on + strand, within MIT1002_00420 at 455.057 kb on - strand, within MIT1002_00420 at 455.087 kb on + strand at 455.087 kb on + strand at 455.087 kb on + strand at 455.088 kb on + strand at 455.191 kb on + strand at 455.224 kb on - strand at 455.227 kb on + strand at 455.235 kb on - strand at 455.278 kb on - strand at 455.383 kb on + strand, within MIT1002_00421 at 455.388 kb on + strand, within MIT1002_00421 at 455.433 kb on + strand, within MIT1002_00421 at 455.485 kb on - strand, within MIT1002_00421 at 455.485 kb on - strand, within MIT1002_00421 at 455.487 kb on + strand, within MIT1002_00421 at 455.639 kb on + strand, within MIT1002_00421 at 455.639 kb on + strand, within MIT1002_00421 at 455.647 kb on - strand, within MIT1002_00421 at 455.669 kb on + strand, within MIT1002_00421 at 455.684 kb on - strand, within MIT1002_00421 at 455.684 kb on - strand, within MIT1002_00421 at 455.762 kb on + strand, within MIT1002_00421 at 455.775 kb on - strand, within MIT1002_00421 at 455.825 kb on + strand, within MIT1002_00421 at 455.839 kb on + strand, within MIT1002_00421 at 455.847 kb on - strand, within MIT1002_00421 at 456.055 kb on - strand, within MIT1002_00421 at 456.078 kb on + strand, within MIT1002_00421 at 456.098 kb on - strand, within MIT1002_00421 at 456.099 kb on - strand, within MIT1002_00421 at 456.148 kb on + strand, within MIT1002_00421 at 456.156 kb on - strand, within MIT1002_00421
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 6 remove 453,268 + MIT1002_00419 0.53 +0.3 453,287 + MIT1002_00419 0.54 -0.2 453,287 + MIT1002_00419 0.54 -0.2 453,292 + MIT1002_00419 0.54 -1.0 453,295 - MIT1002_00419 0.54 -1.2 453,316 - MIT1002_00419 0.55 +2.5 453,316 - MIT1002_00419 0.55 -1.4 453,358 + MIT1002_00419 0.58 -1.8 453,490 + MIT1002_00419 0.66 -0.5 453,525 + MIT1002_00419 0.68 -0.1 453,574 - MIT1002_00419 0.71 -0.2 453,580 - MIT1002_00419 0.71 -2.4 453,709 + MIT1002_00419 0.79 -0.3 453,719 + MIT1002_00419 0.79 +1.3 453,824 + MIT1002_00419 0.85 -1.3 453,832 - MIT1002_00419 0.86 +1.1 453,832 - MIT1002_00419 0.86 +2.0 453,845 + MIT1002_00419 0.87 -2.8 453,853 - MIT1002_00419 0.87 -0.0 453,858 - MIT1002_00419 0.87 +1.7 454,006 + +1.7 454,006 + -0.3 454,014 - +2.0 454,065 + +1.4 454,076 - +1.5 454,097 + +0.5 454,144 + +1.7 454,144 - -1.3 454,217 + -0.6 454,225 - +0.5 454,231 + -0.1 454,247 - -0.0 454,273 + -0.2 454,281 - -0.1 454,281 - -0.6 454,303 + -0.2 454,303 + -0.8 454,453 + MIT1002_00420 0.22 +0.2 454,459 - MIT1002_00420 0.22 -0.9 454,590 + MIT1002_00420 0.36 -2.2 454,647 + MIT1002_00420 0.43 -1.3 454,647 + MIT1002_00420 0.43 +0.2 454,665 + MIT1002_00420 0.44 -1.8 454,665 + MIT1002_00420 0.44 -0.2 454,673 - MIT1002_00420 0.45 -1.1 454,680 - MIT1002_00420 0.46 +0.7 454,778 - MIT1002_00420 0.57 -0.8 454,799 + MIT1002_00420 0.59 +0.0 454,807 - MIT1002_00420 0.60 -0.3 454,815 - MIT1002_00420 0.61 -1.9 454,905 + MIT1002_00420 0.70 +1.0 454,912 + MIT1002_00420 0.71 -0.1 454,920 - MIT1002_00420 0.72 +1.0 454,955 + MIT1002_00420 0.76 +2.8 454,982 - MIT1002_00420 0.79 +1.3 454,999 - MIT1002_00420 0.80 +0.8 455,028 + MIT1002_00420 0.84 -0.7 455,028 + MIT1002_00420 0.84 -1.3 455,057 - MIT1002_00420 0.87 +1.4 455,087 + -0.3 455,087 + -1.8 455,087 + -1.2 455,088 + +2.4 455,191 + -0.4 455,224 - +0.2 455,227 + -0.5 455,235 - -1.0 455,278 - +1.8 455,383 + MIT1002_00421 0.16 -1.0 455,388 + MIT1002_00421 0.17 -0.3 455,433 + MIT1002_00421 0.21 -2.1 455,485 - MIT1002_00421 0.26 +0.7 455,485 - MIT1002_00421 0.26 +1.1 455,487 + MIT1002_00421 0.26 -0.5 455,639 + MIT1002_00421 0.40 -0.2 455,639 + MIT1002_00421 0.40 +0.1 455,647 - MIT1002_00421 0.41 +1.0 455,669 + MIT1002_00421 0.43 -0.1 455,684 - MIT1002_00421 0.44 -0.2 455,684 - MIT1002_00421 0.44 +0.1 455,762 + MIT1002_00421 0.52 -0.8 455,775 - MIT1002_00421 0.53 +0.7 455,825 + MIT1002_00421 0.57 -0.5 455,839 + MIT1002_00421 0.59 +0.1 455,847 - MIT1002_00421 0.59 -1.0 456,055 - MIT1002_00421 0.79 -2.3 456,078 + MIT1002_00421 0.81 -1.0 456,098 - MIT1002_00421 0.83 -0.5 456,099 - MIT1002_00421 0.83 -0.8 456,148 + MIT1002_00421 0.88 +0.2 456,156 - MIT1002_00421 0.88 +0.7
Or see this region's nucleotide sequence